Microbial Genomics最新文献_第2页

Evaluation of nationwide analysis surveillance for methicillin-resistant Staphylococcus aureus within Genomic Medicine Sweden.

IF 4 2区生物学

Microbial Genomics Pub Date : 2025-01-01 DOI: 10.1099/mgen.0.001331

Erika Tång Hallbäck, Jonas T Björkman, Fredrik Dyrkell, Jenny Welander, Hong Fang, Isak Sylvin, Ren Kaden, Hinnerk Eilers, Anna Söderlund Strand, Sara Mernelius, Linda Berglind, Amaya Campillay Lagos, Lars Engstrand, Per Sikora, Paula Mölling

{"title":"Evaluation of nationwide analysis surveillance for methicillin-resistant Staphylococcus aureus within Genomic Medicine Sweden.","authors":"Erika Tång Hallbäck, Jonas T Björkman, Fredrik Dyrkell, Jenny Welander, Hong Fang, Isak Sylvin, Ren Kaden, Hinnerk Eilers, Anna Söderlund Strand, Sara Mernelius, Linda Berglind, Amaya Campillay Lagos, Lars Engstrand, Per Sikora, Paula Mölling","doi":"10.1099/mgen.0.001331","DOIUrl":"10.1099/mgen.0.001331","url":null,"abstract":"Background. National epidemiological investigations of microbial infections greatly benefit from the increased information gained by whole-genome sequencing (WGS) in combination with standardized approaches for data sharing and analysis.Aim. To evaluate the quality and accuracy of WGS data generated by different laboratories but analysed by joint pipelines to reach a national surveillance approach.Methods. A national methicillin-resistant Staphylococcus aureus (MRSA) collection of 20 strains was distributed to nine participating laboratories that performed in-house procedures for WGS. Raw data were shared and analysed by three pipelines: 1928 Diagnostics, JASEN (GMS pipeline) and CLC-Genomics Workbench. The outcomes were compared according to quality, correct strain identification and genetic distances.Results. One isolate contained intraspecies contamination and was excluded from further analysis. The mean sequencing depth varied between sites and technologies. However, all analysis methods identified 12 strains that belonged to one of five outbreak clusters. The cut-off definition was set to <10 allele differences for core genome multilocus sequence typing (cgMLST) and <20 genetic differences for SNP analysis in a pairwise comparison.Conclusions. MRSA isolates, which are whole genome sequenced by different laboratories and analysed using the same bioinformatic pipelines, yielded comparable results for outbreak clustering for both cgMLST and SNP, using the 1928 analysis pipeline. In this study, JASEN was best suited to analyse Illumina data and CLC to analyse within respective technology. In the future, real-time sharing of data and harmonized analysis within the Genomic Medicine Sweden consortium will further facilitate investigations of outbreaks and transmission routes.","PeriodicalId":18487,"journal":{"name":"Microbial Genomics","volume":"11 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution and spread of Xanthomonas citri subsp. citri in the São Paulo, Brazil, citrus belt inferred from 758 novel genomes. 柑橘黄单胞菌亚种的进化与传播。从758个新的基因组中推断出巴西圣保罗的柑橘带。

IF 4 2区生物学

Microbial Genomics Pub Date : 2025-01-01 DOI: 10.1099/mgen.0.001338

Caio Felipe Cavicchia Zamunér, Dennis Carhuaricra-Huaman, Roobinidevi Ragupathy, James Redfern, Carmen L Rodriguez-Cueva, Franklin Behlau, Mark C Enright, Henrique Ferreira, João C Setubal

{"title":"Evolution and spread of Xanthomonas citri subsp. citri in the São Paulo, Brazil, citrus belt inferred from 758 novel genomes.","authors":"Caio Felipe Cavicchia Zamunér, Dennis Carhuaricra-Huaman, Roobinidevi Ragupathy, James Redfern, Carmen L Rodriguez-Cueva, Franklin Behlau, Mark C Enright, Henrique Ferreira, João C Setubal","doi":"10.1099/mgen.0.001338","DOIUrl":"10.1099/mgen.0.001338","url":null,"abstract":"The São Paulo state citrus belt in Brazil is a major citrus production region. Since at least 1957, citrus plantations in this region have been affected by citrus canker, an economically damaging disease caused by Xanthomonas citri subsp. citri (Xcc). For about 50 years, until 2017, a citrus canker eradication programme was carried out in this region. In this work, our aim was to investigate the effects of the eradication programme on genetic variability and evolution of Xcc. To this end, we sequenced and analysed 758 Xcc genomes sampled in the São Paulo citrus belt, together with 730 publicly available Xcc genomes from around the world. Our phylogenomic analyses show that these genomes can be grouped into seven major lineages and that in São Paulo, lineage L7 is dominant. Our time estimate for its appearance closely matches the date when citrus production expanded. L7 can be subdivided into lineages L7.1 and L7.2. In our samples, L7.2, which we estimate to have emerged around 1964, is by far the most abundant, showing that the eradication programme had little impact on strain diversification. On the other hand, oscillations in the estimated effective population size of L7.2 strains over time closely match the shifts in the eradication programme. In sum, we present a detailed view of the genomic diversity of Xcc in the world and in São Paulo, the largest such effort in terms of a number of genomes for a crop pathogen undertaken so far. The methods employed here can form the basis for active genomic surveillance of Xcc in major citrus production areas.","PeriodicalId":18487,"journal":{"name":"Microbial Genomics","volume":"11 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecological insights into the microbiology of food using metagenomics and its potential surveillance applications. 利用宏基因组学对食品微生物学的生态学见解及其潜在的监测应用。

IF 4 2区生物学

Microbial Genomics Pub Date : 2025-01-01 DOI: 10.1099/mgen.0.001337

Samuel J Bloomfield, Falk Hildebrand, Aldert L Zomer, Raphaëlle Palau, Alison E Mather

{"title":"Ecological insights into the microbiology of food using metagenomics and its potential surveillance applications.","authors":"Samuel J Bloomfield, Falk Hildebrand, Aldert L Zomer, Raphaëlle Palau, Alison E Mather","doi":"10.1099/mgen.0.001337","DOIUrl":"10.1099/mgen.0.001337","url":null,"abstract":"A diverse array of micro-organisms can be found on food, including those that are pathogenic or resistant to antimicrobial drugs. Metagenomics involves extracting and sequencing the DNA of all micro-organisms on a sample, and here, we used a combination of culture and culture-independent approaches to investigate the microbial ecology of food to assess the potential application of metagenomics for the microbial surveillance of food. We cultured common foodborne pathogens and other organisms including Escherichia coli, Klebsiella/Raoultella spp., Salmonella spp. and Vibrio spp. from five different food commodities and compared their genomes to the microbial communities obtained by metagenomic sequencing following host (food) DNA depletion. The microbial populations of retail food were found to be predominated by psychrotrophic bacteria, driven by the cool temperatures in which the food products are stored. Pathogens accounted for a small percentage of the food metagenome compared to the psychrotrophic bacteria, and cultured pathogens were inconsistently identified in the metagenome data. The microbial composition of food varied amongst different commodities, and metagenomics was able to classify the taxonomic origin of 59% of antimicrobial resistance genes (ARGs) found on food to the genus level, but it was unclear what percentage of ARGs were associated with mobile genetic elements and thus transferable to other bacteria. Metagenomics may be used to survey the ARG burden, composition and carriage on foods to which consumers are exposed. However, food metagenomics, even after depleting host DNA, inconsistently identifies pathogens without enrichment or further bait capture.","PeriodicalId":18487,"journal":{"name":"Microbial Genomics","volume":"11 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142922048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli from humans and a river in Aotearoa New Zealand. 新西兰奥特罗阿地区人类和一条河流中产生β -内酰胺酶的大肠杆菌的基因组流行病学研究。

IF 4 2区生物学

Microbial Genomics Pub Date : 2025-01-01 DOI: 10.1099/mgen.0.001341

Holly A Gray, Patrick J Biggs, Anne C Midwinter, Lynn E Rogers, Ahmed Fayaz, Rukhshana N Akhter, Sara A Burgess

{"title":"Genomic epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli from humans and a river in Aotearoa New Zealand.","authors":"Holly A Gray, Patrick J Biggs, Anne C Midwinter, Lynn E Rogers, Ahmed Fayaz, Rukhshana N Akhter, Sara A Burgess","doi":"10.1099/mgen.0.001341","DOIUrl":"10.1099/mgen.0.001341","url":null,"abstract":"In Aotearoa New Zealand, urinary tract infections in humans are commonly caused by extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli. This group of antimicrobial-resistant bacteria are often multidrug resistant. However, there is limited information on ESBL-producing E. coli found in the environment and their link with human clinical isolates. In this study, we examined the genetic relationship between environmental and human clinical ESBL-producing E. coli and isolates collected in parallel within the same area over 14 months. Environmental samples were collected from treated effluent, stormwater and multiple locations along an Aotearoa New Zealand river. Treated effluent, stormwater and river water sourced downstream of the treated effluent outlet were the main samples that were positive for ESBL-producing E. coli (7/14 samples, 50.0%; 3/6 samples, 50%; and 15/28 samples, 54%, respectively). Whole-genome sequence comparison was carried out on 307 human clinical and 45 environmental ESBL-producing E. coli isolates. Sequence type 131 was dominant for both clinical (147/307, 47.9%) and environmental isolates (11/45, 24.4%). Only one ESBL gene was detected in each isolate. Among the clinical isolates, the most prevalent ESBL genes were bla CTX-M-27 (134/307, 43.6%) and bla CTX-M-15 (134/307, 43.6%). Among the environmental isolates, bla CTX-M-15 (28/45, 62.2%) was the most prevalent gene. A core SNP analysis of these isolates suggested that some strains were shared between humans and the local river. These results highlight the importance of understanding different transmission pathways for the spread of ESBL-producing E. coli.","PeriodicalId":18487,"journal":{"name":"Microbial Genomics","volume":"11 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11718517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Towards quantifying plasmid similarity. 勘误：对定量质粒相似性。

IF 4 2区生物学

Microbial Genomics Pub Date : 2024-12-01 DOI: 10.1099/mgen.0.001329

William Matlock, Liam P Shaw, Samuel K Sheppard, Edward Feil

引用次数: 0

Non-toxigenic cases of Vibrio cholerae in Spain from 2012 to 2022. 2012 - 2022年西班牙霍乱弧菌非产毒性病例。

IF 4 2区生物学

Microbial Genomics Pub Date : 2024-12-01 DOI: 10.1099/mgen.0.001315

Camille Jacqueline, Sergio Román Soto, Silvia Herrera-Leon

{"title":"Non-toxigenic cases of Vibrio cholerae in Spain from 2012 to 2022.","authors":"Camille Jacqueline, Sergio Román Soto, Silvia Herrera-Leon","doi":"10.1099/mgen.0.001315","DOIUrl":"10.1099/mgen.0.001315","url":null,"abstract":"Non-toxigenic non-O1/non-O139 Vibrio cholerae (NVC) isolates are associated with diarrhoeal disease globally. NVC-related infections are on the rise, representing one of the most striking examples of emerging human diseases linked to climate change. This study aims to give a better picture of the evolution of NCV incidence in Spain from 2012 to 2022. In this context, we realized a descriptive analysis and a logistic regression using the isolates submitted to the National Center of Microbiology (NCM) during this period. To elucidate the heterogeneity of sporadic clinical strains of NVC among patients residing in Spain, we conducted whole-genome sequencing (WGS) of a selection of isolates. First, we observed an increase in the number of isolates sent to the NCM after 2019, which was not concomitant to a change in the national surveillance protocol. Furthermore, the number of cases and hospitalizations increased with age. Second, we found a high diversity of NVC strains, which suggested that the usefulness of WGS studies might be limited in waterborne outbreak situations to find the infectious source. Finally, we characterized the genetic determinants responsible for antimicrobial resistance and virulence and found that 21% of the isolates were resistant to β-lactamases. To the best of our knowledge, the present study is the first in Spain to report genomic data on non-toxigenic cases at the national level. Because of the high percentage of hospitalization observed for NVC cases (40%), it might be beneficial to test for V. cholerae in all the suspected cases.","PeriodicalId":18487,"journal":{"name":"Microbial Genomics","volume":"10 12","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptococcus suis infection on European farms is associated with an altered tonsil microbiome and resistome. 欧洲农场的猪链球菌感染与扁桃体微生物组和抵抗组的改变有关。

IF 4 2区生物学

Microbial Genomics Pub Date : 2024-12-01 DOI: 10.1099/mgen.0.001334

Simen Fredriksen, Carlos Neila-Ibáñez, Isabel Hennig-Pauka, Xiaonan Guan, Jenelle Dunkelberger, Isabela Fernandes de Oliveira, Maria Laura Ferrando, Florencia Correa-Fiz, Virginia Aragon, Jos Boekhorst, Peter van Baarlen, Jerry M Wells

{"title":"Streptococcus suis infection on European farms is associated with an altered tonsil microbiome and resistome.","authors":"Simen Fredriksen, Carlos Neila-Ibáñez, Isabel Hennig-Pauka, Xiaonan Guan, Jenelle Dunkelberger, Isabela Fernandes de Oliveira, Maria Laura Ferrando, Florencia Correa-Fiz, Virginia Aragon, Jos Boekhorst, Peter van Baarlen, Jerry M Wells","doi":"10.1099/mgen.0.001334","DOIUrl":"10.1099/mgen.0.001334","url":null,"abstract":"Streptococcus suis is a Gram-positive opportunistic pathogen causing systemic disease in piglets around weaning age. The factors predisposing to disease are not known. We hypothesized that the tonsillar microbiota might influence disease risk via colonization resistance and/or co-infections. We conducted a cross-sectional case-control study within outbreak farms complemented by selective longitudinal sampling and comparison with control farms without disease occurrence. We found a small but significant difference in tonsil microbiota composition between case and control piglets (n=45+45). Variants of putative commensal taxa, including Rothia nasimurium, were reduced in abundance in case piglets compared to asymptomatic controls. Case piglets had higher relative abundances of Fusobacterium gastrosuis, Bacteroides heparinolyticus and uncultured Prevotella and Alloprevotella species. Piglets developing disease post-weaning had reduced alpha diversity pre-weaning. Despite case-control pairs receiving equal antimicrobial treatment, case piglets had a higher abundance of antimicrobial resistance genes conferring resistance to antimicrobial classes used to treat S. suis. This might be an adaption of disease-associated strains to frequent antimicrobial treatment.","PeriodicalId":18487,"journal":{"name":"Microbial Genomics","volume":"10 12","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The primate gut microbiota contributes to interspecific differences in host metabolism. 灵长类动物肠道微生物群有助于宿主代谢的种间差异。

IF 4 2区生物学

Microbial Genomics Pub Date : 2024-12-01 DOI: 10.1099/mgen.0.001322

Elizabeth K Mallott, Sahana Kuthyar, Won Lee, Derek Reiman, Hongmei Jiang, Sriram Chitta, E Alexandria Waters, Brian T Layden, Ronen Sumagin, Laura D Manzanares, Guan-Yu Yang, Maria Luisa Savo Sardaro, Stanton Gray, Lawrence E Williams, Yang Dai, James P Curley, Chad R Haney, Emma R Liechty, Christopher W Kuzawa, Katherine R Amato

{"title":"The primate gut microbiota contributes to interspecific differences in host metabolism.","authors":"Elizabeth K Mallott, Sahana Kuthyar, Won Lee, Derek Reiman, Hongmei Jiang, Sriram Chitta, E Alexandria Waters, Brian T Layden, Ronen Sumagin, Laura D Manzanares, Guan-Yu Yang, Maria Luisa Savo Sardaro, Stanton Gray, Lawrence E Williams, Yang Dai, James P Curley, Chad R Haney, Emma R Liechty, Christopher W Kuzawa, Katherine R Amato","doi":"10.1099/mgen.0.001322","DOIUrl":"https://doi.org/10.1099/mgen.0.001322","url":null,"abstract":"Because large brains are energetically expensive, they are associated with metabolic traits that facilitate energy availability across vertebrates. However, the biological underpinnings driving these traits are not known. Given its role in regulating host metabolism in disease studies, we hypothesized that the gut microbiome contributes to variation in normal cross-vertebrate species differences in metabolism, including those associated with the brain's energetic requirements. By inoculating germ-free mice with the gut microbiota (GM) of three primate species - two with relatively larger brains and one with a smaller brain - we demonstrated that the GM of larger-brained primates shifts host metabolism towards energy use and production, while that of smaller-brained primates stimulates energy storage in adipose tissues. Our findings establish a causal role of the GM in normal cross-host species differences in metabolism associated with relative brain size and suggest that the GM may have been an important facilitator of metabolic changes during human evolution that supported encephalization.","PeriodicalId":18487,"journal":{"name":"Microbial Genomics","volume":"10 12","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global genomic epidemiology of bla _GES-5 carbapenemase-associated integrons. bla GES-5碳青霉烯酶相关整合子的全球基因组流行病学研究

IF 4 2区生物学

Microbial Genomics Pub Date : 2024-12-01 DOI: 10.1099/mgen.0.001312

William Matlock, Liam P Shaw, Nicole Stoesser

{"title":"Global genomic epidemiology of bla GES-5 carbapenemase-associated integrons.","authors":"William Matlock, Liam P Shaw, Nicole Stoesser","doi":"10.1099/mgen.0.001312","DOIUrl":"10.1099/mgen.0.001312","url":null,"abstract":"Antimicrobial resistance (AMR) gene cassettes comprise an AMR gene flanked by short recombination sites (attI and attC or attC and attC). Integrons are genetic elements able to capture, excise and shuffle these cassettes, providing 'adaptation on demand', and can be found on both chromosomes and plasmids. Understanding the patterns of integron diversity may help to understand the epidemiology of AMR genes. As a case study, we examined the clinical resistance gene bla GES-5, an integron-associated class A carbapenemase first reported in Greece in 2004 and since observed worldwide, which to our knowledge has not been the subject of a previous global analysis. Using a dataset comprising all de-duplicated NCBI contigs containing bla GES-5 (n=104), we developed a pangenome graph-based workflow to characterize and cluster the diversity of bla GES-5-associated integrons. We demonstrate that bla GES-5-associated integrons on plasmids are different to those on chromosomes. Chromosomal integrons were almost all identified in Pseudomonas aeruginosa ST235, with a consistent gene cassette content and order. We observed instances where insertion sequence IS110 disrupted attC sites, which might immobilize the gene cassettes and explain the conserved integron structure despite the presence of intI1 integrase promoters, which would typically facilitate capture or excision and rearrangement. The plasmid-associated integrons were more diverse in their gene cassette content and order, which could be an indication of greater integrase activity and 'shuffling' of integrons on plasmids.","PeriodicalId":18487,"journal":{"name":"Microbial Genomics","volume":"10 12","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11616780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secretor status is a modifier of vaginal microbiota-associated preterm birth risk. 分泌物状态是阴道微生物群相关早产风险的修饰因子。

IF 4 2区生物学

Microbial Genomics Pub Date : 2024-12-01 DOI: 10.1099/mgen.0.001323

Samit Kundu, Gonçalo Dos Santos Correia, Yun S Lee, Sherrianne Ng, Lynne Sykes, Denise Chan, Holly Lewis, Richard G Brown, Lindsay Kindinger, Anne Dell, Ten Feizi, Stuart M Haslam, Yan Liu, Julian R Marchesi, David A MacIntyre, Phillip R Bennett

{"title":"Secretor status is a modifier of vaginal microbiota-associated preterm birth risk.","authors":"Samit Kundu, Gonçalo Dos Santos Correia, Yun S Lee, Sherrianne Ng, Lynne Sykes, Denise Chan, Holly Lewis, Richard G Brown, Lindsay Kindinger, Anne Dell, Ten Feizi, Stuart M Haslam, Yan Liu, Julian R Marchesi, David A MacIntyre, Phillip R Bennett","doi":"10.1099/mgen.0.001323","DOIUrl":"10.1099/mgen.0.001323","url":null,"abstract":"Mutations in the FUT2 gene that result in a lack of expression of histo-blood group antigens on secreted glycoproteins may shape the vaginal microbiota with consequences for birth outcome. To test this, we analysed the relationship between secretor status, vaginal microbiota and gestational length in an ethnically diverse cohort of 302 pregnant women, including 82 who delivered preterm. Lactobacillus gasseri and L. jensenii were found to have distinct co-occurrence patterns with other microbial taxa in non-secretors. Moreover, non-secretors with Lactobacillus spp. depleted high diversity vaginal microbiota in early pregnancy had significantly shorter gestational length than Lactobacillus spp. dominated non-secretors (mean of 241.54 days (sd=47.14) versus 266.21 (23.61); P-value=0.0251). Similar gestational length differences were observed between non-secretors with high vaginal diversity and secretors with Lactobacillus spp. dominance (mean of 262.52 days (SD=27.73); p-value=0.0439) or depletion (mean of 266.05 days (SD=20.81); p-value=0.0312). Our data highlight secretor status and blood-group antigen expression as being important mediators of vaginal microbiota-host interactions in the context of preterm birth risk.","PeriodicalId":18487,"journal":{"name":"Microbial Genomics","volume":"10 12","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11616779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0