Nanotechnology Development最新文献_第2页

Nano/microwires of coronene for sensing electron-deficient aromatics 传感缺电子芳烃的冠烯纳米/微线

Nanotechnology Development Pub Date : 2011-12-09 DOI: 10.4081/ND.2012.E1

Hong Wang, Xiaohe Xu, H. Ji

引用次数: 1

Taking a precautionary approach to nanotechnology 对纳米技术采取预防措施

Nanotechnology Development Pub Date : 2011-10-27 DOI: 10.4081/ND.2011.E6

D. O’Mathúna

引用次数: 3

Quantum-well thickness dependence of spin polarization of excitons 激子自旋极化的量子阱厚度依赖性

Nanotechnology Development Pub Date : 2011-10-20 DOI: 10.4081/ND.2011.E5

M. I. Miah

引用次数: 0

Nevirapine nanosuspension: comparative investigation of production methods 奈韦拉平纳米混悬液:生产方法的比较研究

Nanotechnology Development Pub Date : 2011-10-12 DOI: 10.4081/ND.2011.E4

R. Shegokar, K. Singh, R. Mueller

{"title":"Nevirapine nanosuspension: comparative investigation of production methods","authors":"R. Shegokar, K. Singh, R. Mueller","doi":"10.4081/ND.2011.E4","DOIUrl":"https://doi.org/10.4081/ND.2011.E4","url":null,"abstract":"Increasing number of antiretroviral drugs coming from high throughput screening besides their high dose has poor solubility profile. Formulation development of these drugs is a major obstacle to their clinical application. To overcome extremely low water solubility and associated poor bioavailability they can be formulated as nanosuspensions. This paper is not only focuses on production of parenteral nevirapine nanosuspensions but also on scaling up of formulations for clinical use. Lab scale (APV LAB 40, 40 mL) and medium scale (Avestin C50, 2 kg) production was performed using piston gap high pressure homogenization (HPH), while the feasibility for pilot scale up was checked using a bead milling technique in continuous mode (PM, Buhler PML-2). Nanosuspension was characterized for particle sizes, zeta potential, crystallanity and stability. The mean particle sizes for lab scale, medium scale and pilot scale production obtained were 481 nm, 429 nm and 211 nm, respectively. Independent of the production method (lab and pilot scale) all processed formulations showed more or less similar zeta potential (~15 mV) in conductivity adjusted water. Long term stability over 1 year showed significant increase in particle size at all storage conditions for lab scale and medium scale production (high energy size reduction) whereas they remained physically stable (with negligible increase) for the milled product (low energy size reduction). As the technology has been scaled up successfully for nevirapine nanosuspension, the product can be considered for commercial exploitation. The prepared nevirapine nanosuspensions can be administered for parenteral or oral use.","PeriodicalId":184845,"journal":{"name":"Nanotechnology Development","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127797078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Recombinant human arginase I immobilized on gold and silver nanoparticles: preparation and properties 金、银纳米颗粒固定化重组人精氨酸酶I:制备及性能研究

Nanotechnology Development Pub Date : 2011-09-08 DOI: 10.4081/ND.2011.E3

N. Stasyuk, R. Serkiz, S. Mudry, G. Gayda, Andriy Zakalskiy, Yevgen Koval’chuk, M. Gonchar, M. Nisnevitch

{"title":"Recombinant human arginase I immobilized on gold and silver nanoparticles: preparation and properties","authors":"N. Stasyuk, R. Serkiz, S. Mudry, G. Gayda, Andriy Zakalskiy, Yevgen Koval’chuk, M. Gonchar, M. Nisnevitch","doi":"10.4081/ND.2011.E3","DOIUrl":"https://doi.org/10.4081/ND.2011.E3","url":null,"abstract":"Metal nanoparticles (NPs), such as gold (Au) and silver (Ag), are important for chemistry, physics, and biology due to their unique optical, electrical, and photothermal properties. Such NPs are widely used for immobilization of various bioactive substances, including peptides, enzymes, antibodies and DNA. The synthesis of silver and gold nanoparticles was carried out by reduction of silver nitrate by glucose and reduction of tetrachloroauric acid by sodium citrate, respectively. The size and structure of the AgNPs and AuNPs were characterized using TEM, AFM and XRD methods. The average size of the AgNPs and AuNPs was between 8 and 15 nm. Recombinant arginase I was immobilized using the carbodiimidepentafluorophenol method on the surface of NPs functionalized with ω-mercaptohexadecanoic acid. It was shown that recombinant human liver arginase I isolated from the yeast Hansenula polymorpha maintains satisfactory stability after immobilization on both NPs. The immobilized arginase retained 40% of its activity on the surface of AuNPs and 25% on AgNPs compared to the free arginase after storage at +4 oC during 25 days. The immobilized enzyme can be used for assay of arginine in pharmaceuticals, in food products and in blood.","PeriodicalId":184845,"journal":{"name":"Nanotechnology Development","volume":"2015 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128051380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Health technology assessment processes for nanotechnologies: the ethical domain 纳米技术的卫生技术评估过程:伦理领域

Nanotechnology Development Pub Date : 2011-08-12 DOI: 10.4081/ND.2011.E1

A. Spagnolo, P. Refolo, D. Sacchini, V. Daloiso

引用次数: 2

Albumin coated liposomes: a novel platform for macrophage specific drug delivery 白蛋白包被脂质体:巨噬细胞特异性药物递送的新平台

Nanotechnology Development Pub Date : 2011-07-19 DOI: 10.4081/ND.2011.E2

Clément Vuarchey, Sushil Kumar, R. Schwendener

{"title":"Albumin coated liposomes: a novel platform for macrophage specific drug delivery","authors":"Clément Vuarchey, Sushil Kumar, R. Schwendener","doi":"10.4081/ND.2011.E2","DOIUrl":"https://doi.org/10.4081/ND.2011.E2","url":null,"abstract":"Here we report a new and efficient approach of macrophage specific drug delivery by coating liposomes with albumin. Activated albumin was reacted with liposomes containing polyethylene glycol (PEG) as hydrophilic spacers to create a flexible layer of covalently bound albumin molecules on the liposome surface. Albumin coated liposomes were taken up faster and more efficiently than uncoated liposomes by murine macrophages. Liposome uptake was significantly higher in macropha ges as compared to other cell types tested (endothelial cells, fibroblasts, tumor cells), suggesting specificity for macrophages. In vivo, splenic macrophages phagocytosed BSA coated liposomes (BSA-L) at faster rates compared to conventional liposomes (L) and PEG liposomes (PEG-L). To prove the effectiveness of this new macrophage specific drug carrier, the bisphosphonates clodronate and zoledronate were encapsulated in BSA-L and compared with conventional liposomes. In vitro, treatment of macrophages with clodronate or zoledronate in BSA-L led to cytotoxic activity within a very short time and to up to 50-fold reduced IC50 concentrations. In vivo, clodronate encapsulated in BSA-L depleted splenic macrophages at a 5-fold lower concentration as conventional clodronate-liposomes. Our results highlight the pharmaceutical benefits of albumin-coated liposomes for macrophage specific drug delivery.","PeriodicalId":184845,"journal":{"name":"Nanotechnology Development","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132624088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14