Methods in molecular medicine最新文献_第5页

Computer-assisted reading of DNA sequences. 计算机辅助读取DNA序列。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-60327-148-6_10

Huong Le, Marcus Hinchcliffe, Bing Yu, Ronald J A Trent

引用次数: 5

Three methods to assay inhibitors of ribosomal subunit assembly. 测定核糖体亚基组装抑制剂的三种方法。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_6

W Scott Champney

引用次数: 5

Assays for the identification of inhibitors targeting specific translational steps. 鉴定针对特定翻译步骤的抑制剂的试验。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_8

Letizia Brandi, John Dresios, Claudio O Gualerzi

{"title":"Assays for the identification of inhibitors targeting specific translational steps.","authors":"Letizia Brandi, John Dresios, Claudio O Gualerzi","doi":"10.1007/978-1-59745-246-5_8","DOIUrl":"https://doi.org/10.1007/978-1-59745-246-5_8","url":null,"abstract":"While bacterial protein synthesis is the target of about half of the known antibiotics, the great structural-functional complexity of the translational machinery still offers remarkable opportunities for identifying novel and specific inhibitors of unexploited targets. We designed a knowledge-based in vitro translation assay to identify inhibitors selectively targeting the bacterial or the yeast translational apparatus, preferentially blocking the early steps of protein synthesis. Using a natural-like, \"universal\" model mRNA and cell-free extracts prepared from Eschericha coli, Saccharomyces cerevisiae, and HeLa cells, we were able to translate, with comparable yields in the three systems, the immunogenic peptide encoded by this \"universal\" mRNA. The immuno-enzymatic quantification of the translated peptide in the presence of a potential inhibitor can identify a selective bacterial or fungal inhibitor inactive in the human system. When applied to the high-throughput screening (HTS) of a library of approximately 25,000 natural products, this assay led to the identification of two novel and specific inhibitors of bacterial translation.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":"142 ","pages":"87-105"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-59745-246-5_8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27404317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

In situ Hybridization. 原位杂交。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_27

Kayhan T Nouri-Aria

{"title":"In situ Hybridization.","authors":"Kayhan T Nouri-Aria","doi":"10.1007/978-1-59745-366-0_27","DOIUrl":"https://doi.org/10.1007/978-1-59745-366-0_27","url":null,"abstract":"Hybridization is the formation of hybrid nucleic acid molecules with complementary nucleotide sequences in DNA:DNA, DNA:RNA, or RNA:RNA forms. In situ hybridization is a highly sensitive technique that allows detection and localization of specific DNA or RNA molecules in morphologically preserved isolated cells, histological tissue sections, or chromosome preparations. In situ hybridization has broad range of applications and has been used to (a) localize viral infection, (b) identify sites of gene expression, (c) analyze mRNA transcription and tissue distribution, and (d) map gene sequences in chromosomes. There are several advantages of the use of in situ hybridization including the fact that it can be applied to archival materials and frozen tissues and can be combined with immunohistochemistry to detect protein as well as mRNA of interest or phenotype of cells expressing the target genome, detecting more than one nucleic acid sequences using different labeling methods.The major steps involved in in situ hybridization are as follows: probe preparation and labeling, tissue fixation, permeabilization, hybridization, and signal detection and these are described in detail in this chapter.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":"138 ","pages":"331-47"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-59745-366-0_27","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27522175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Ultrasensitive ELISA for measurement of human cytokine responses in primary culture. 测定人原代培养细胞因子反应的超灵敏ELISA。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_10

William P Stefura, J Darren Campbell, Renée Douville, Monique J Stinson, F Estelle Simons, Allan B Becker, Kent T HayGlass

引用次数: 25

Quantification of human chemokine production in TLR-stimulated and antigen-specific recall responses. tlr刺激和抗原特异性召回反应中人类趋化因子产生的定量。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_11

Monique Stinson, Renee Douville, Yuriy Lissitsyn, Melanie Blanchard, William Stefura, Estelle Simons, Allan Becker, Peter Nickerson, Kevin Coombs, Kent HayGlass

{"title":"Quantification of human chemokine production in TLR-stimulated and antigen-specific recall responses.","authors":"Monique Stinson, Renee Douville, Yuriy Lissitsyn, Melanie Blanchard, William Stefura, Estelle Simons, Allan Becker, Peter Nickerson, Kevin Coombs, Kent HayGlass","doi":"10.1007/978-1-59745-366-0_11","DOIUrl":"https://doi.org/10.1007/978-1-59745-366-0_11","url":null,"abstract":"Chemokines are primarily low molecular mass proteins that are produced and usually released by a wide variety of cell types. Differential chemokine responses can be excellent early markers of immune dysfunction, allowing clinical intervention prior to expression of full blown undesirable effector responses. Thus, assessment of the nature and intensity of Ag-dependent chemokine production provides a valuable tool for probing human immune regulation.Here, we provide detailed instructions on approaches we have developed to assess the nature and intensity of recall responses to a wide variety of exogenous and endogenous antigens capable of consistently stimulating chemokine responses by PBMC from adult and pediatric populations. This chapter is divided into two sections. The first is focused on culture techniques for eliciting antigen-driven chemokine responses for a panel of chemokines that are relevant to immune function. The second section details assay systems for their quantitative analysis.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":"138 ","pages":"121-31"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27522382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Isolation, flow cytometric analysis, and suppression assay of CD4+ CD25+ T-regulatory cells. CD4+ CD25+ t调节细胞的分离、流式细胞术分析和抑制实验。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_8

Hayley Jeal

引用次数: 2

Methods to identify and characterize inhibitors of bacterial RNA polymerase. 方法鉴定和表征细菌RNA聚合酶抑制剂。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_4

A Simon Lynch, Qun Du

引用次数: 3

Statistical analysis of image data provided by two-dimensional gel electrophoresis for discovery proteomics. 对二维凝胶电泳提供的图像数据进行统计分析，发现蛋白质组学。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-60327-148-6_15

Ben Crossett, Alistair V G Edwards, Melanie Y White, Stuart J Cordwell