Membrane biochemistry最新文献_第5页

Enzymatic lysis of sulfated glycosaminoglycans reduces the electrophoretic mobility of vascular endothelial cells. 酶解磺化糖胺聚糖降低血管内皮细胞的电泳迁移率。

Membrane biochemistry Pub Date : 1990-04-01 DOI: 10.3109/09687689009025831

F F Vargas, H M Osorio, C Basilio, M De Jesus, U S Ryan

{"title":"Enzymatic lysis of sulfated glycosaminoglycans reduces the electrophoretic mobility of vascular endothelial cells.","authors":"F F Vargas, H M Osorio, C Basilio, M De Jesus, U S Ryan","doi":"10.3109/09687689009025831","DOIUrl":"https://doi.org/10.3109/09687689009025831","url":null,"abstract":"The main purpose of this work was to identify the macromolecules carrying the surface charge of endothelial cells. This was done by measuring changes in cell electrophoretic mobility caused by enzymatic removal of glycocalyx components. Endothelial cells were removed from the bovine pulmonary artery using nonenzymatic procedures, plated, and identified by immunocytochemical methods and electron microscopy. Cultured cells were suspended in saline and placed in the lumen of a capillary in a Rank Brothers electrophoresis instrument. Voltage was applied between the ends of the capillary, and the velocity acquired by the cells was measured with a microscope. Preincubating the cells in protein-free saline for 1 h reduced the mobility by 25%. This reflects the loss of proteoheparan sulfate from the cell surface. Cell mobility was totally suppressed by exposing the entire cell surface to chondroitin sulfate lyase, but it was only slightly diminished when the enzyme was applied only to the cell side facing the culture medium. A partial decrease in mobility was obtained after enzymatic removal of either heparin, heparan sulfate, or collagen. The results indicate that sulfated glycosaminoglycans are the main carriers of the surface change in vascular endothelial cells. The asymmetrical effect of chondroitinase on the two sides of the cell indicates a distribution polarization for glycosaminoglycans in endothelial cells.","PeriodicalId":18448,"journal":{"name":"Membrane biochemistry","volume":"9 2","pages":"83-9"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687689009025831","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13259119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Identification of a spectroscopic marker for the Ca2(+)-binding site of (Ca2+ + Mg2+)-ATPase of sarcoplasmic reticulum in the occluded state. 肌浆网(Ca2+ + Mg2+)- atp酶在闭塞状态下Ca2(+)结合位点的光谱标记物鉴定

Membrane biochemistry Pub Date : 1990-01-01 DOI: 10.3109/09687689009026823

T P Lockwich, A E Shamoo

引用次数: 0

Molecular mechanisms of regulation of neuronal muscarinic receptor sensitivity. 神经元毒蕈碱受体敏感性调控的分子机制。

Membrane biochemistry Pub Date : 1990-01-01 DOI: 10.3109/09687689009026820

E E el-Fakahany, C L Cioffi

引用次数: 30

Direct hyposmotic stimulation of gastric acid secretion. 直接低渗刺激胃酸分泌。

Membrane biochemistry Pub Date : 1990-01-01 DOI: 10.3109/09687689009026819

T J Sernka

引用次数: 9

An Escherichia coli mutant conditionally altered in respiratory chain components. 一种有条件改变呼吸链成分的大肠杆菌突变体。

Membrane biochemistry Pub Date : 1990-01-01 DOI: 10.3109/09687689009026822

J C Cox, P Jurtshuk

{"title":"An Escherichia coli mutant conditionally altered in respiratory chain components.","authors":"J C Cox, P Jurtshuk","doi":"10.3109/09687689009026822","DOIUrl":"https://doi.org/10.3109/09687689009026822","url":null,"abstract":"Nitrosoguanidine mutagenesis was employed to isolate an Escherichia coli mutant conditionally altered in respiratory chain components. Mutant R25 was able to grow on glucose, fructose, and glycerol but failed to grow on succinate and acetate (suc-). Also, R25 exhibited leaky growth on DL-lactate, fumarate, and malate (lct*). The lct* mutation pleiotropically affected a number of respiratory chain components and its expression was conditional with the growth substrate. Glucose-grown R25 resting cell suspensions oxidized DL-lactate and formate; however, these two substrates were not oxidized by fructose- or glycerol-grown cell suspensions. The same conditional pattern was observed for the concentration of cytochrome components, the membrane-associated oxidation of NADH and formate, and formate phenazine methosulfate (PMS) reductase activity; succinate oxidase and PMS reductase activities were not exhibited by membranes under any growth condition due to the suc- mutation. R25 membrane-associated H(+)-translocating ATPase activity was not conditional with the growth substrate. R25PC, a spontaneous lct+ suc- partial revertant of R25, did not exhibit the conditional pattern of R25. The lct* mutation was found to map in the 27-30-min region and the suc- mutation in the 15-17-min region of the E. coli genome. Two distinct classes of R25 P1kc transductants were isolated that differed in both their growth response on succinate and DL-lactate and their oxidase activities.","PeriodicalId":18448,"journal":{"name":"Membrane biochemistry","volume":"9 1","pages":"47-60"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687689009026822","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13304769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison of the biosynthesis and composition of polyglycerophosphatides and phosphatidylinositols in mitochondria and microsomes isolated from neonatal and adult rat heart and liver. 新生儿和成年大鼠心脏和肝脏线粒体和微粒体中聚甘油磷脂和磷脂酰肌醇的生物合成和组成的比较。

Membrane biochemistry Pub Date : 1990-01-01 DOI: 10.3109/09687689009026821

L Stuhne-Sekalec, M Wassenaar, G Jackowski, N Z Stanacev

{"title":"Comparison of the biosynthesis and composition of polyglycerophosphatides and phosphatidylinositols in mitochondria and microsomes isolated from neonatal and adult rat heart and liver.","authors":"L Stuhne-Sekalec, M Wassenaar, G Jackowski, N Z Stanacev","doi":"10.3109/09687689009026821","DOIUrl":"https://doi.org/10.3109/09687689009026821","url":null,"abstract":"The level of biosynthesis and the composition of polyglycerophosphatides (phosphatidylglycerol, phosphatidyglycerolphosphate, and diphosphatidylglycerol or cardiolipin) and phosphatidylinositols were examined in mitochondria and microsomes, respectively, isolated from neonatal and adult rat heart and liver. Biosynthesis of [3H]polyglycerophosphatides [( 3H]phosphatidylglycerol and [3H]phosphatidylglycerolphosphate) was 4.5 times higher in neonatal than in adult heart mitochondria, whereas in the respective liver mitochondria this synthesis was only 15% higher in neonatal mitochondria. The biosynthesis of [3H]phosphatidylinositol was twice as high in neonatal as in adult heart microsomes, but very similar in the respective liver microsomes. The major biosynthesized polyglycerophosphatide was [3H]phosphatidylglycerol. The accumulation of [3H]phosphatidylglycerolphosphate depended on the origin of the mitochondria. Under our experimental conditions [3H]phosphatidylinositol was the only synthesized phosphoinositide in all microsomes. The biosynthesis of cardiolipin depended on the origin of the mitochondria and was highest in adult rat liver mitochondria and lowest in adult heart mitochondria. In all cases the biosynthesized [14C,3H] cardiolipin from [14C]phosphatidylglycerol and [3H]CDP-diglycerides had a ratio of 14C/3H around unity. The biosynthesis of [3H]CDP-diglycerides, the key precursor for the biosynthesis of phosphatidylglycerol, phosphatidylinositol, and cardiolipin, was 30% higher in neonatal than in adult heart microsomes and very similar in the respective liver microsomes. The subcellular localization of the enzymes required for the biosynthesis of the lipids and liponucleotides examined was found to be the same in membranes isolated from neonatal and adult rat heart and liver.","PeriodicalId":18448,"journal":{"name":"Membrane biochemistry","volume":"9 1","pages":"29-45"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687689009026821","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13233647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Stimulation of canine kidney BBMV ATPase activity by acidic pH in the presence of Zn2+: an ATPase activity distinct from transport ATPases and alkaline phosphatase that may be an ecto-ATPase. Zn2+存在下的酸性pH对犬肾BBMV atp酶活性的刺激:一种不同于转运atp酶和碱性磷酸酶的atp酶活性，可能是一种外源性atp酶。

Membrane biochemistry Pub Date : 1990-01-01 DOI: 10.3109/09687689009026824

S A Hilden, N E Madias

{"title":"Stimulation of canine kidney BBMV ATPase activity by acidic pH in the presence of Zn2+: an ATPase activity distinct from transport ATPases and alkaline phosphatase that may be an ecto-ATPase.","authors":"S A Hilden, N E Madias","doi":"10.3109/09687689009026824","DOIUrl":"https://doi.org/10.3109/09687689009026824","url":null,"abstract":"Renal brush border membrane vesicles (BBMV) of the dog possess at least two ATPase activities. In the present study, we have examined the effect of pH, ions, and inhibitors on the activity of ATPase in BBMV. Two different sets of conditions were identified that produced stimulation of ATPase activity. A unique stimulation of BBMV ATPase activity occurred at acidic pH in the presence of 1 mM ZnCl2. In the absence of Zn2+, a second ATPase activity was stimulated by alkaline pH values with peak stimulation occurring between pH 8.5 and 9.0. The results suggest that the alkaline pH-stimulated hydrolysis of ATP probably represents the activity of BBMV alkaline phosphatase. The unique acidic pH + Zn2(+)-stimulated ATPase activity must represent the activity of a second protein other than the alkaline phosphatase, since purified alkaline phosphatase did not show this activity. The biochemical identity and physiological function of this renal BBMV ATPase activity remain to be determined, but it may be an ecto-ATPase.","PeriodicalId":18448,"journal":{"name":"Membrane biochemistry","volume":"9 1","pages":"69-81"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687689009026824","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13305298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Histamine-mediated hyposmotic stimulation of gastric acid secretion. 组胺介导的胃酸分泌低渗刺激。

Membrane biochemistry Pub Date : 1989-01-01 DOI: 10.3109/09687688909082264

T J Sernka

引用次数: 4

A procedure for purification of the ryanodine receptor from skeletal muscle. 从骨骼肌中提纯良诺定受体的方法。

Membrane biochemistry Pub Date : 1989-01-01 DOI: 10.3109/09687688909025827

M J Hawkes, M Díaz-Muñoz, S L Hamilton

引用次数: 32

Cooperative binding of primycin and gramicidin on erythrocyte membranes. A cation transport study. 普里霉素和革兰霉素在红细胞膜上的协同结合。阳离子输运研究。

Membrane biochemistry Pub Date : 1989-01-01 DOI: 10.3109/09687688909025821

I P Suga'r, K Blaskó, S Györgyi, L V Shcagina, V V Malev, A A Lev

引用次数: 1