Dabao Yin, Yingtong Mu, Xue Li, Mei Hua1, Xiaojie Li, Peiqing Zhang, Xiaoming Zhang, Junjie Wang
{"title":"Comparison of the Complete Chloroplast Genomes in the Astragalus","authors":"Dabao Yin, Yingtong Mu, Xue Li, Mei Hua1, Xiaojie Li, Peiqing Zhang, Xiaoming Zhang, Junjie Wang","doi":"10.18805/lrf-763","DOIUrl":"https://doi.org/10.18805/lrf-763","url":null,"abstract":"Background: Astragalus is one of the largest angiosperms and has important economic value. The chloroplast genomes (Cp) of most plants in its family have been sequenced and annotated, but there are fewer studies on the characteristics of the chloroplast genomes and codon usage bias of Astragalus. In this study, we sequenced and annotated the complete chloroplast genomes of three Astragalus species, that systematically compared the chloroplast genome and codon usage characteristics of three Astragalus. Methods: In this study, we used three Astragalus species as materials. Firstly, we sequenced and assembled their chloroplast genomes. Subsequently, we analyzed the codon usage bias of the chloroplast genomes of the three medicinal Astragalus species using software such as CodonW, CUSP and SPSS. Result: The results illustrated that the length of Astragalus chloroplast genome ranges from 122,815 bp (A. dahuricus) to 123,729 bp (A. melilotoides Pall.). There were 107-110genes, including 75-76 protein-coding genes (PCGs), four ribosomal RNA genes (rRNAs) and 28-30 transfer RNA genes (tRNAs). In addition, the results illustrated that codons in three Cp genomes from Astragalus had the same 11 Optimal codons and ending with A/U. The codon usage frequency of five model creatures were compared and the results revealed that the codon preferences of Astragalus and five model creatures were pretty significant, the natural selection was the main factor in codon preference. Our research provides some value for the researches of chloroplast genetic engineering and molecular breeding in Astragalus.\u0000","PeriodicalId":17998,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"55 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139441823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Arya, S.B. Mishra, Kanshouwa Modunshim Maring, Ravi Kant
{"title":"Stability Analysis for Biological Nitrogen Fixation and Seed Yield in Mungbean [Vigna radiata (L.) Wilczek] Genotypes","authors":"M. Arya, S.B. Mishra, Kanshouwa Modunshim Maring, Ravi Kant","doi":"10.18805/lr-5256","DOIUrl":"https://doi.org/10.18805/lr-5256","url":null,"abstract":"Background: Mungbean is a versatile legume crop grown in various cropping systems. Despite its adaptability, the yield is hindered by the low yielding capacity of undifferentiated varieties and their limited ability to fix nitrogen. The current study aims to identify stable genotypes through continuous cultivation in eight different environments over two years. Methods: To assess the stability for BNF and seed yield, twenty-five mungbean genotypes were measured at eight diverse agro-ecological conditions, represented as two different seasons during spring and summer in two consecutive years (2016 and 2017) at two locations - TCA, Dholi, Muzaffarpur and Pusa Farm, RPCAU, Pusa, Samastipur, Bihar. To evaluate the most stable and superior genotypes, three parameters, viz., , bi and S2di were calculated. Result: There is a substantial amount of variability across genotypes and environments, as shown by the pooled analysis of variance. The genotype, Pusa 11-31 had shown regression near to unity, comprised with non-significant standard deviation along with higher mean seed yield and biological nitrogen fixation indicates that this is the most stable genotypes for the above traits.\u0000","PeriodicalId":17998,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"46 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139447406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An Efficient Hairy Root Transformation Method for Common Bean based on Petiole Explants","authors":"Y.H. Liu, X.Q. Cai, K. Ning, P. Xu","doi":"10.18805/lrf-778","DOIUrl":"https://doi.org/10.18805/lrf-778","url":null,"abstract":"Background: Common bean (Phaseolus vulgaris L.) is a major food legume with high nutritional value and economic importance globally. A remarkable attribute of this plant is its propensity to generate adventitious roots from petioles of detached leaves when moisture is appropriate. This distinctive feature presents promising prospects for harnessing petioles in the facilitation of transgenic hairy root production. Methods: We achieved the successful induction of transgenic hairy roots from common bean petioles through the utilization of Agrobacterium rhizogenes strain K599. Our experimentation encompassed a diverse array of common bean varieties and leaves of varying ages. Subsequently, we refined and optimized the procedure for hairy root induction. Result: Our investigations have revealed that the most conducive conditions for hairy root transformation with K599 are attained using 5-day-old leaves from the cultivar ‘Honghuaqingjia’, exhibiting a remarkable induction efficiency of 59%. The usefulness of this system was demonstrated through a subcellular localization analysis of the transcription factor PvTCP2 protein in combination with GFP (Green Fluorescent Protein).\u0000","PeriodicalId":17998,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"78 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139452245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of Utilizing Different Types of Organic and Bio-Fertilizers on Growth, Elements Concentration, Seed Yield and Essential Oil of Coriandrum sativum L.","authors":"H. G. Tabasi, M. Darzi, M. S. Hadi","doi":"10.18805/lrf-770","DOIUrl":"https://doi.org/10.18805/lrf-770","url":null,"abstract":"Background: Bio-fertilizers may be used to maintain soil fertility and soil improvement. Methods: In this study involving 12 treatments and 3 replications was conducted in Tehran, Iran. The experiment was conducted in 2018–2019 as a randomized complete block design. The treatments were 1- vermicompost (10 t/ha), 2- nitroxin, 3- bio-superphosphate, 4- biosulfur, 5- vermicompost + nitroxin, 6- vermicompost + bio-superphosphate, 7- vermicompost + biosulfur, 8- nitroxin + bio-superphosphate, 9- nitroxin + biosulfur, 10- bio-superphosphate + biosulfur, 11- chemical fertilizer and 12- control (without fertilizer). Result: The results showed that the treatments had a significant impact on the characteristics examined such as the highest plant height (75.3 cm) in the treatment combining vermicompost and biological superphosphate and the highest number of shoots/plant. The highest concentration of N in seeds (2.92%) was obtained when plants were treated with nitroxin and biosulfur and the highest concentration of P in seeds (0.83%) when treated with biological superphosphate. In addition, the highest K content in seeds (4.06%) and grain yield (1297.5 kg.ha-1) were found in the treatment of nitroxin and bio-superphosphate, as well as the content of essential oil (0.32%). and the yield of essential oils (2.89 kg.ha-1) was obtained when treated with nitroxin. Consequently, Bio-organic fertilizers can be considered as an alternative to chemical fertilizers in organic coriander cultivation.","PeriodicalId":17998,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"29 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139161633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hong-Tao Xiang, Li Wan, Liu Jia, He Ning, Xue-Yang Wang, Li Bo, Hongchang Xie, Deming Wang
{"title":"Effect of Exogenous Abscisic Acid (ABA), a Potential Growth Regulator on Physiological Response to Chilling Stress of Adzuki Bean (Vigna angularis) at Flowering Stage","authors":"Hong-Tao Xiang, Li Wan, Liu Jia, He Ning, Xue-Yang Wang, Li Bo, Hongchang Xie, Deming Wang","doi":"10.18805/lrf-748","DOIUrl":"https://doi.org/10.18805/lrf-748","url":null,"abstract":"Background: Low temperatures during flowering severely affect crop growth and yield. Methods: This experiment was conducted under potted conditions with LXD 4 and TJH as test materials and pre-sprayed with exogenous ABA at a concentration of 20 mg·L-1 at flowering stage, respectively and then with an average of 15°C condition for 5 days. Result: The results showed that foliar spraying exogenous ABA effectively inhibited the increase of MDA content and relatively significantly improved the photosynthetic parameters such as Gs and Tr and alleviated the loss of yield factors caused by chilling stress. Compared with spraying water, spraying exogenous ABA can significantly increase the yield per pot of LXD 4 by 6.72~17.77% and TJH by 6.41~37.04% under low temperature conditions. In summary, foliar spraying exogenous ABA ameliorated the effects of chilling stress on the physiological characteristics of adzuki bean leaves and improved yield as a result of improved antioxidant defense mechanisms which impeded lipid peroxidation. Thus, we conclude that foliar spraying exogenous ABA could decelerate the damages caused by chilling stress to some extent.\u0000","PeriodicalId":17998,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"4 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138945106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Subbiah, M. Prabhu, A. R. Kumar, K. Indhumathi, R. Jagadeesan, A. Nithyadevi, A. Vijayasamundeeswari
{"title":"Studies on the Influence Age of Rootstocks and Season on Grafting Success in Manila Tamarind (Pithecellobium dulce Roxb.)","authors":"A. Subbiah, M. Prabhu, A. R. Kumar, K. Indhumathi, R. Jagadeesan, A. Nithyadevi, A. Vijayasamundeeswari","doi":"10.18805/lr-5244","DOIUrl":"https://doi.org/10.18805/lr-5244","url":null,"abstract":"Background: Manila tamarind (Pithecellobium dulce (Roxb.) Benth) belongs to the family Fabaceae and sub-family Mimosoideae. This tree have multiple uses; fruit, firewood, honey, fodder, soap oil, tannin, hedges and shade. Naturally this plant species can be multiplied by seeds. Apart from genetic variability, the seedling progenies may take long time to first bearing and sometimes irregular bearing also noticed due to its genetic makeup. In order to overcome these drawbacks, vegetative propagation is the only alternate way to get genetically uniform, early bearing and high yielding characters. The age of rootstocks and season of grafting influences the success of the grafts and performance of the grafted plants. With this background, studies on age of rootstocks and season on grafting success in Manila Tamarind was carried out. Methods: An experiment was conducted to assess the effect of season and age of the rootstocks in Manila Tamarind (Pithecellobium dulce). The experiment was laid out in factorial randomized block design with 15 treatment combinations and four replications. In this study, three rootstocks of different age viz., 6, 9 and 12 months old were grafted during June, July, August, September and October. Result: The main and interaction effects on age of rootstocks and season of grafting had a significant influence on survival percentage, number of days taken to first sprouting, number of leaves per plant and chlorophyll content of manila tamarind under controlled conditions. The softwood grafting performed on nine month old rootstocks during September recorded the highest survival percentage and it was significantly different from all other treatments. The same treatment i.e., nine months old rootstock grafted in September was also recorded the less number of days taken to first sprouting with highest number of leaves per plant and chlorophyll content.","PeriodicalId":17998,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"36 34","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138994245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shushuan Wang, Fengling Shi, Ruru Shi, Yutong Zhang
{"title":"Seed Dormancy and Germination in Alfalfa (Medicago falcata L.)","authors":"Shushuan Wang, Fengling Shi, Ruru Shi, Yutong Zhang","doi":"10.18805/lrf-758","DOIUrl":"https://doi.org/10.18805/lrf-758","url":null,"abstract":"Background: Medicago falcata L. (M. falcata L.) is widely distributed in Xinjiang and Inner Mongolia, which is a significant source of resistance genes. Its seeds have a high level of physical dormancy (PD). The current study aimed to study the dormancy characteristics, water absorption characteristics and methods for releasing hard seeds of M. falcata L., to reveal the the relationship between seed coat structure and water absorption and to understand the dormancy characteristics, which can provide theoretical basis for seed dormancy and seed biology research. Methods: Seed germination rate (GR), absorption, germination inhibitor activity and initial site of water entry into seeds were measured to investigate the causes of seed dormancy. Treatments to break dormancy included: (1) sandpaper sanding, (2) ultrasonic treatment for 30 min after sandpaper sanding, (3) 98% sulfuric acid immersion (5,10, 20, 40, 60 and 120 min) and (4) hot water immersion at different temperatures (50, 60, 70, 80 and 90oC) for 10 min, 20 min and 30 min. Result: The results showed that the hard rate of untreated seeds was 99.33% and the hard rate after the seed coat was scratched was 1.33%. Based on the inhibitory activity test of M. falcata L. seeds, high concentration seed extract has an inhibitory effect on the growth of other seeds. The toludine staining test showed that the hilum and microscope are the initial and main parts for seed water absorption. The germination rate of seeds after soaking in 98% sulfuric acid for 40 minutes and ultrasonic treatment with sandpaper for 30 minutes is higher than 94%, which is an effective method to relieve the PY of M. falcata L. seeds.","PeriodicalId":17998,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"31 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138955667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B.R. Sharanya, A.P. Mallikarjuna Gowda, R. Muthuraju, K.N. Srinivasappa, B.G. Vasanthi, K. Pushpa
{"title":"Identification of Native Root Nodulating Bacteria from Mucuna (Mucuna pruriens L.)","authors":"B.R. Sharanya, A.P. Mallikarjuna Gowda, R. Muthuraju, K.N. Srinivasappa, B.G. Vasanthi, K. Pushpa","doi":"10.18805/lr-5210","DOIUrl":"https://doi.org/10.18805/lr-5210","url":null,"abstract":"Background: Mucuna (Mucuna pruriens L.) is an annual herbaceous climber, grown as a medicinal, green manure, cover and smothering crop. The mucuna seeds contains L-DOPA (L-3, 4 dihydroxy phenylalanine), a non-protein amino acid, extensively used for Parkinson and hypertensive drug. Injudicious application of nitrogenous fertilizers leads to deterioration of soil quality which results into loss of crop yield and quality. The application of microbial inoculant containing efficient native rhizobia enhances the nodular properties, N2-fixation and soil quality. Therefore, Rhizobium strain associated with mucuna was isolated, biochemically characterized and identified. The 16SrRNA sequencing revealed that Sinnorhizobium mililoti, a gram negative symbiotic nitrogen fixing bacteria is present in root nodules of mucuna. Methods: Root nodules were extracted from mucuna grown at ICAR- Krishi Vigyan Kendra, Doddaballapura Taluk, Bengaluru Rural District, Karnataka, then cultured, screened and characterized in the laboratory. The 16SrRNA sequencing and phylogenetic analysis was done to identify the native rhizobial strain. Result: Identification of native root nodulating bacteria through 16SrRNA sequencing concluded that Sinnorhizobium mililoti strain associated with the root nodules of Mucuna (Mucuna pruriens L.).\u0000","PeriodicalId":17998,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138962792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Roy, A. Sarkar, S. Pavithra, P. Bhaumik, P.M. Bhattachraya, N. Sahana
{"title":"Screening of Lentil Germplasm against Stemphylium Blight and Studies on Association between Disease and Biochemical Parameters","authors":"A. Roy, A. Sarkar, S. Pavithra, P. Bhaumik, P.M. Bhattachraya, N. Sahana","doi":"10.18805/lr-5185","DOIUrl":"https://doi.org/10.18805/lr-5185","url":null,"abstract":"Background: Stemphylium blight, an important fungal disease of lentil caused by Stemphylium botryosum Wallr, has been reported to cause an estimated yield loss of 62% in India and Bangladesh. Considering the importance of the crop vis-à-vis the severity of the disease under terai agro-climatic region of West Bengal, the present study was undertaken to evaluate the germplasm of lentil for Stemphylium blight resistance for their utilization in future resistance breeding programme. Methods: The present experiment was carried out during the rabi season of 2019-20 and 2020-21 with forty lentil germplasm including two checks viz; WBL77 and IPL220 in an alpha lattice design under field condition and in completely randomized design under artificial inoculation respectively. Statistical analysis was carried out using R v. 4.1.1 software. Result: ANOVA revealed significant variation among the genotypes for the studied characters. PCV was found to be higher than GCV for all the studied characters. High heritability (broad sense) accompanied with high genetic advance as percent of mean indicated the presence of additive type of gene action. Based on per cent disease index germplasm were categorized as resistant or moderately resistant types for both under field condition and artificial inoculation. AUDPC was significantly but negatively associated with change in phenol and change in OD phenol under field condition same as that was observed between AUDPC and PPO content under artificial inoculation. Above study state that selection based on disease reaction and their associated parameters gives fruitful result to provide disease resistance parent against Stemphylium blight.\u0000","PeriodicalId":17998,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"2 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138586252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of Cowpea-maize Intercropping and Bio-rational Product on Fall Armyworm, Spodoptera frugiperda (JE Smith) in Fodder Maize","authors":"Subash Singh, Sohan Singh Walia, Kuldeep Singh Bhullar","doi":"10.18805/lr-5199","DOIUrl":"https://doi.org/10.18805/lr-5199","url":null,"abstract":"Background: A recent occurrence of fall armyworm (FAW), Spodoptera frugiperda (JE Smith) a new invasive pest in Africa attained epidemic form in 2016. Consequently, researchers probed various ecofriendly methods to manage the pest. The effect of intercropping cowpea [Vigna unguiculata (L.) Walp.] with fodder maize (Zea mays L.) on the incidence of FAW was investigated. Methods: The maize fodder was protected against FAW by intercropping with cowpea along with a biorational product, Brahamastra and compared with the recommended insecticide used to manage the insect by conducting field experiments during kharif 2020, 2021 and 2022 comprising the eight treatments viz. T1: Maize + Cowpea 1:1 ratio (unsprayed); T2: Maize + Cowpea 1:1 ratio (coragen @ 100 ml ha-1); T3: Maize + Cowpea 1:1 ratio (brahamastra @ 12.5 litre ha-1 sprayed); T4: Maize sole (brahamastra @ 7.5 litre ha-1 sprayed); T5: Maize sole (brahamastra @ 10.0 litre ha-1 sprayed); T6: Maize sole (Brahamastra @ 12.5 litre ha-1 sprayed); T7: Maize sole (coragen 18.5 SC @ 100 ml ha-1 sprayed as standard check) and T8: Maize sole (Unsprayed). The plot size for each treatment was 500 m2, demarcating 5 ´ 4 m2 for sampling with three replications in a completely randomized design (CRBD). Result: The treatments, Maize + Cowpea (1:1 row) (Coragen @ 100 ml ha-1 sprayed) and Maize sole (Coragen @ 100 ml ha-1 sprayed) though had significantly the lowest pooled pest incidence and the highest per cent reduction over control (PROC), the bio-rational applied on maize + Cowpea (1:1 row) (brahamastra @ 12.5 litre ha-1 sprayed) encounted the lowest pest incidence, the highest PROC combined with the highest fodder yield and economic returns without any statistical singnificant difference with Maize + Cowpea (1:1 row) (unsprayed).\u0000","PeriodicalId":17998,"journal":{"name":"LEGUME RESEARCH - AN INTERNATIONAL JOURNAL","volume":"35 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138588975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}