{"title":"Detection of Foot and Mouth Disease Virus in Salted Raw Cowhide from Malaysia in Tanjung Priok Port, Indonesia","authors":"Disty Ayu Sekarsana, C. Basri, D. Lukman","doi":"10.20473/jmv.vol7.iss1.2024.48-56","DOIUrl":"https://doi.org/10.20473/jmv.vol7.iss1.2024.48-56","url":null,"abstract":"Animal products such as raw salted cowhide are thought to have the potential to transmit the foot and mouth disease (FMD) virus from the infected zone. Indonesia imports raw salted cowhide from Malaysia, so it has the potential to transmit FMD to Indonesia which enters through Tanjung Priok Port. This study aimed to investigate the presence of the FMD virus in raw salted cowhide from Malaysia. The number of samples was collected from each container of raw salted cowhide imported through Tanjung Priok Port during August–December 2022. A total of 21 samples were obtained from 21 bulk containers containing raw salted cowhide. Real time q Polymerase Chain Reaction (RT-qPCR) was used to investigate foot and mouth disease virus in samples. The RT-qPCR screening test on 21 samples reported that salted raw cowhide was free from the FMD virus. Continuous monitoring and surveillance protocols for salted rawhide imported from non-free countries need to be carried out at other points of entry.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"185 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140760226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yolla Rona Mustika, M. Effendi, Yulianna Puspitasari, H. Plumeriastuti, A. Khairullah, Kurnia Nisa Kinasih
{"title":"Identification of Escherichia coli Multidrug Resistance in Cattle in Abattoirs","authors":"Yolla Rona Mustika, M. Effendi, Yulianna Puspitasari, H. Plumeriastuti, A. Khairullah, Kurnia Nisa Kinasih","doi":"10.20473/jmv.vol7.iss1.2024.19-32","DOIUrl":"https://doi.org/10.20473/jmv.vol7.iss1.2024.19-32","url":null,"abstract":"Escherichia coli is a typical flora of an animals' digestive tract. Based on these details, this study was done on the detection and identification of multidrug-resistant E. coli in cattle in Surabaya abattoirs. Each cattle rectum swab sample was streaked into EMB agar media and followed by Gram staining. The IMViC test was used to confirm the presence of E. coli. Based on morphological culture features, Gram staining, and biochemical testing, the sample examination results revealed that 41 samples (41%) of the 100 cattle rectal swab samples that were isolated were confirmed to be positive for E. coli. The profile of antibiotic resistance from the results of the E. coli resistance test to antibiotics showed that out of a total of 41 isolates of E. coli, 12 isolates (29.27%) were detected as resistant to 1 class of antibiotics tested, while 2 isolates (4.88%) were resistant to 2 classes of antibiotics, and 3 isolates (7.32%) were confirmed to be multidrug resistance (MDR).","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"6 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140782456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection of Melanocortin Receptor Type 4 (MC4R) Gene in Semen of Etawah Crossbreed and Senduro Goats","authors":"Ristaqul Husna Belgania, Budi Utomo, Imam Mustofa, Yayuk Kholifah, Muhammad Fajar Amrullah","doi":"10.20473/jmv.vol6.iss2.2023.209-215","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss2.2023.209-215","url":null,"abstract":"Etawah crossbreed and Senduro goats as superior local goats and biological genetic resources that must be preserved in Indonesia. Melanocortin receptor type 4 (MC4R) is the primary gene that regulates food intake and energy balance which can be used as a candidate marker for livestock selection genes. This study aimed to analyze the comparison of cement quality and detect the profile of the MC4R gene in Etawah crossbreed and Senduro goat semen. The samples of this study were Etawah crossbreed and Senduro goat semen. Macroscopic examination of cement includes the evaluation of volume, consistency, odor, color, and pH. Microscopic examination of semen includes motility, abnormalities, and spermatozoa concentration. Examination of motility was performed in the condition of fresh semen, after the addition of diluents, and post-thawing of frozen straw. The polymerase chain reaction (PCR) method was performed to amplify specific fragments of the MC4R gene. There was a decrease in spermatozoa motility before freezing and post-thawing frozen semen compared to fresh semen. The quality of frozen straw post-thawing Etawah crossbreed and Senduro goat was still worth using for artificial insemination and in vitro embryo production. The MC4R gene can be detected in liquid and frozen semen from Etawah crossbreed and Senduro goats using a PCR test in the form of a single band along 642 bp.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135462870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kadek Leni Martha Diana, Putu Devi Jayanti, I Wayan Batan
{"title":"Rhipicephalus sanguineus Infestation with Ehrlichiosis in Shitzu-Pomeranian Crossbred Dogs Treated using Red Fermented Rice: A Case Report","authors":"Kadek Leni Martha Diana, Putu Devi Jayanti, I Wayan Batan","doi":"10.20473/jmv.vol6.iss2.2023.303-311","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss2.2023.303-311","url":null,"abstract":"This report aimed to provide information on the diagnosis, treatment, and therapeutic evaluation of Ehrlichiosis in crossbreed dogs treated using red fermented rice. A Shitzu-Pomeranian crossbreed was indicated with decreased appetite, weight loss, and the presence of high levels of pruritus due to suffering from blood parasites. Physical examination showed an infestation of Rhipicephalus sanguineus around the eyes and back area. A complete hematological examination and blood smear showed leukocytosis, lymphocytosis, thrombocytopenia, increased granulocytes, and the presence of intracytoplasmic bodies in monocytes. The results of the examination using the test kit showed that the sample was positive for Ehrlichiosis. Treatment using Doxycycline (5 mg/kg BW, for 28 days), Ivermectin 1% (0,2 ml SC), Diphenhydramine HCl 10 mg/kg (1,1 ml IM), Chlorpheniramine maleate (1 tablet/day for 14 days), Vitamin B12 (1 tablet/day for 28 days), and 5 ml of red fermented rice solution twice a day for 21 days. After 21 days, the whole treatment reported the appetite returned to normal, reduced infestation of R. sanguineus, and reduced frequency of pruritus.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"35 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135463043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Made Gede Adi Surya Saputra, I Wayan Sudira, Samsuri Samsuri, I Made Merdana, Anak Agung Gde Oka Dharmayudha, I Gusti Ngurah Sudisma
{"title":"Evaluation of Acute Dermal Toxicity of Hibiscus Leaves as Simplicial Ointment on Albino Rats","authors":"Made Gede Adi Surya Saputra, I Wayan Sudira, Samsuri Samsuri, I Made Merdana, Anak Agung Gde Oka Dharmayudha, I Gusti Ngurah Sudisma","doi":"10.20473/jmv.vol6.iss2.2023.216-229","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss2.2023.216-229","url":null,"abstract":"Hibiscus is a plant that has been shown to contain substances that may be used as sunscreen to protect the skin from UV radiation. In terms of their potential as sunscreens, flavonoids, tannins, and quinone chemicals are crucial because they are antioxidants that can reduce the negative effects of UV radiation and boost photoprotective activity. This study aimed to investigate the acute dermal toxicity potential of hibiscus leaves simplicial ointment 40% on female albino rats. The evaluation method refered to The OECD Guideline for Testing of Chemicals – No. Test: 402, Acute Dermal Toxicity-Fixed Doses Procedure. Based on the Globally Harmonized System compared to this study reported that acute dermal median lethal dose (LD50) was > 2000 mg/kg. It can be concluded that hibiscus leaves simplicial ointment 40% has safe to apply topically and doesn't produce acute skin toxicity.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"77 10","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135463046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Hematological Levels of Simmental-Ongole Crossbreed (SimPO) and Ongole Crossbreed (PO) Cattle Reared Semi-Intensively","authors":"Dwi Kristanto, Septiyani Septiyani","doi":"10.20473/jmv.vol6.iss2.2023.237-243","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss2.2023.237-243","url":null,"abstract":"Hematological analysis in cattle is an important basis for determining physiological conditions, biomedical study, and veterinary clinical diagnosis. This study aimed to determine the comparison of hematological values between Simmental-Ongole crossbreed (SimPO) and Ongole crossbreed (PO) cattle reared semi-intensively in oil palm plantations area. A total of 70 blood samples were collected from 35 female SimPO and 35 female PO cattle aged ± 3,5 years who reared from the same area. Blood samples were collected via the coccygeal vein as much as 3 ml, performed at 07.00 in the morning before being grazed on oil palm plantations. Blood samples were collected using a vacutainer tube containing Ethylene Diamine Tetra-Acetic Acid (EDTA) for further analysis using a hematology analyzer. Data from hematological examination results were then analyzed using an independent sample t-test with a significance level of p < 0,05. In results, reported that there were no significant differences in several hematological parameters that have been analyzed. We can conclude that there was no substantial hematological level in the SimPO and PO cattle reared semi-intensively on oil palm plantations.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"25 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135462869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Foot and Mouth Disease Impact on Milk Productivity and Quality in KUD Kertajaya, Kediri, Indonesia","authors":"Yudit Oktanella, Ayunda Ageng Cahyani, Viski Fitri Hendrawan, Widi Nugroho, Galuh Chandra Agustina","doi":"10.20473/jmv.vol6.iss2.2023.244-249","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss2.2023.244-249","url":null,"abstract":"One of the impacts of foot and mouth disease (FMD) on the dairy farming sector is decreasing the quantity and quality of milk production. This study aimed to determine the impact of FMD by observing the productivity and quality of fresh milk during FMD outbreaks. Sampling was performed to obtain productivity data before and during the FMD outbreak using the cluster sampling method in five areas in KUD Kertajaya, Kediri, Indonesia. The lactoscan test was used to analyze the quality of fresh milk from 50 positive samples. Based on the statistical analysis using the paired parametric T-test, it was reported that there was a significant difference in milk productivity before and during the FMD outbreak (p < 0,05). The results of the Lactoscan test on 50 positive samples showed an average fat content of 4,43%, solid nonfat (SNF) content of 8,54%, specific gravity of 1,028, lactose content of 4,67%, total solids content of 12,97%, added water content of 1,44 and freezing point of -0,54°C. In conclusion, the milk of dairy cows during the FMD outbreak often displayed a quality value of milk that met SNI requirements, despite the fact that there was a major decline in productivity.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"55 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135462879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Palagan Senopati Sewoyo, Anak Agung Ayu Mirah Adi, Ida Bagus Oka Winaya, I Wayan Wirata
{"title":"Mammary Tumors in Dogs, Recent Perspectives and Antiangiogenesis as a Therapeutic Strategy: Literature Study","authors":"Palagan Senopati Sewoyo, Anak Agung Ayu Mirah Adi, Ida Bagus Oka Winaya, I Wayan Wirata","doi":"10.20473/jmv.vol6.iss2.2023.271-287","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss2.2023.271-287","url":null,"abstract":"A particular type of tumor that is frequently detected in female dogs who are sexually active is a mammary tumor. Neoplasia results from DNA-based alterations in cell cycle regulating genes. The mammary gland is prone to the formation of tumors due to its dynamic structure. The development of this tumor is supported by numerous variables. It has been recently discovered that there is substantial evidence linking the BRCA2 gene to the process of cancer. Standard examination techniques, such as fine needle aspiration, histopathology, and immunohistochemistry, are used along with ancillary tests to determine the tumor type and degree of malignancy. The primary treatment option for malignant tumors is surgical resection followed by adjuvant chemotherapy; benign tumors necessitate surgical resection as well. Adjuvant therapy options include hormone therapy and non-steroidal anti-inflammatory medications. Tumor tissue undergoes angiogenesis as it grows and develops to accommodate the abundant supply of nutrients. Therefore, angiogenesis-inhibiting therapies can be utilized to halt the growth of tumor cells. A number of antiangiogenic medications are now being studied in clinical settings on humans, and several more are undergoing trials on animals. In addition to pharmaceuticals, viruses may be used as a therapeutic to block tumor angiogenesis.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"6 2-3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135462881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection of Foot and Mouth Disease Virus in Cattle in Lamongan and Surabaya, Indonesia Using RT-PCR Method","authors":"Zayyin Dinana, Fedik Abdul Rantam, Suwarno Suwarno, Imam Mustofa, Jola Rahmahani, Kusnoto Kusnoto","doi":"10.20473/jmv.vol6.iss2.2023.191-196","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss2.2023.191-196","url":null,"abstract":"Foot and mouth disease (FMD) virus was reported as an outbreak in Indonesia in April 2022 and belonged to serotype O/ME-SA/Ind-2001e is spread in the country. This study aimed to detect the causative agent based on clinical symptoms in cattle that have the vesicle in the mouth and hooves. A total of 25 samples were collected during August 2022 from Lamongan and Surabaya, Indonesia. FMD was identified in 58% (7/12) using reverse transcription polymerase chain reaction (RT-PCR), respectively. The samples were performed using universal primers with 328 bp length for primary diagnosis of FMD. These findings indicate that the spread of FMD viruses is highly contagious, so rapid and accurate diagnosis is needed as an effort to control and monitor FMD viruses.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135463047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Putu Devi Jayanti, I Wayan Nico Fajar Gunawan, Soedarmanto Indarjulianto
{"title":"Haemoplasmosis in A Hypoalbuminemic Cat: A Case Report","authors":"Putu Devi Jayanti, I Wayan Nico Fajar Gunawan, Soedarmanto Indarjulianto","doi":"10.20473/jmv.vol6.iss2.2023.288-296","DOIUrl":"https://doi.org/10.20473/jmv.vol6.iss2.2023.288-296","url":null,"abstract":"Haemoplasmosis is a bacterial infection in erythrocytes caused by Mycoplasma sp. This study aimed to report methods of diagnosis, therapy, and evaluation of therapy in cats with haemoplasmosis. The cat showed decreased appetite, conjunctivitis, presence of mucoid mucus on the eyes and nose, gum anemia with a capillary refill time of more than 2 seconds, high temperature, and weakness. Skin and hair examination reported infestations of Ctenocephalides felis and Lynxacarus sp. on the hair. Routine hematologic examination showed normochromic normocytic anemia, leukocytosis, neutrophilia, eosinophilia, lymphocytosis, monocytosis, and hypoalbuminemia. A blood test examination observed Mycoplasma sp. in erythrocyte cells so the cat was diagnosed with haemoplasmosis. Treatment was performed using Oxytetracycline long-acting at 20 mg/kg BW intramuscularly for 2 weeks and revealed support to recovery during the treatment period.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"35 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135462880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}