Korean Journal of Laboratory Medicine最新文献_第3页

Detection of α-thalassemia-1 Southeast Asian and Thai type deletions and β-thalassemia 3.5-kb deletion by single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting analysis. SYBR Green1单管多重实时PCR检测α-地中海贫血-1东南亚型和泰国型缺失和β-地中海贫血3.5 kb缺失

Korean Journal of Laboratory Medicine Pub Date : 2011-07-01 Epub Date: 2011-06-28 DOI: 10.3343/kjlm.2011.31.3.138

Sakorn Pornprasert, Thanatcha Wiengkum, Sarinee Srithep, Isarapong Chainoi, Panthong Singboottra, Sanchai Wongwiwatthananukit

{"title":"Detection of α-thalassemia-1 Southeast Asian and Thai type deletions and β-thalassemia 3.5-kb deletion by single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting analysis.","authors":"Sakorn Pornprasert, Thanatcha Wiengkum, Sarinee Srithep, Isarapong Chainoi, Panthong Singboottra, Sanchai Wongwiwatthananukit","doi":"10.3343/kjlm.2011.31.3.138","DOIUrl":"https://doi.org/10.3343/kjlm.2011.31.3.138","url":null,"abstract":"Background: Prevention and control of thalassemia requires simple, rapid, and accurate screening tests for carrier couples who are at risk of conceiving fetuses with severe thalassemia.Methods: Single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting (HRM) analysis were used for the identification of α-thalassemia-1 Southeast Asian (SEA) and Thai type deletions and β-thalassemia 3.5-kb gene deletion. The results were compared with those obtained using conventional gap-PCR. DNA samples were derived from 28 normal individuals, 11 individuals with α-thalassemia-1 SEA type deletion, 2 with α-thalassemia-1 Thai type deletion, and 2 with heterozygous β-thalassemia 3.5-kb gene deletion.Results: HRM analysis indicated that the amplified fragments from α-thalassemia-1 SEA type deletion, α-thalassemia-1 Thai type deletion, β-thalassemia 3.5-kb gene deletion, and the wild-type β-globin gene had specific peak heights at mean melting temperature (T(m)) values of 86.89℃, 85.66℃, 77.24℃, and 74.92℃, respectively. The results obtained using single-tube multiplex real-time PCR with SYBR Green1 and HRM analysis showed 100% consistency with those obtained using conventional gap-PCR.Conclusions: Single-tube multiplex real-time PCR with SYBR Green1 and HRM analysis is a potential alternative for routine clinical screening of the common types of α- and β-thalassemia large gene deletions, since it is simple, cost-effective, and highly accurate.","PeriodicalId":17890,"journal":{"name":"Korean Journal of Laboratory Medicine","volume":"31 3","pages":"138-42"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3343/kjlm.2011.31.3.138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30024248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Subcutaneous phaeohyphomycosis caused by Phaeoacremonium species in a kidney transplant patient: the first case in Korea. 肾移植患者皮下嗜绿丝酵素引起的嗜绿丝酵素病:韩国首例。

Korean Journal of Laboratory Medicine Pub Date : 2011-07-01 Epub Date: 2011-06-28 DOI: 10.3343/kjlm.2011.31.3.201

Jonghyeon Choi, Yangsoon Lee, Hae-Sun Chung, Ja-Seung Koo, Dongeun Yong, Yu Sun Kim, Kyungwon Lee, Yunsop Chong

{"title":"Subcutaneous phaeohyphomycosis caused by Phaeoacremonium species in a kidney transplant patient: the first case in Korea.","authors":"Jonghyeon Choi, Yangsoon Lee, Hae-Sun Chung, Ja-Seung Koo, Dongeun Yong, Yu Sun Kim, Kyungwon Lee, Yunsop Chong","doi":"10.3343/kjlm.2011.31.3.201","DOIUrl":"https://doi.org/10.3343/kjlm.2011.31.3.201","url":null,"abstract":"Phaeohyphomycosis is a subcutaneous infection caused by dark pigmented fungi, including fungi of the species Phaeoacremonium, Alternaria, Exophiala, and Pyrenochaeta. In August 2005, a 54-yr-old man who had received a renal transplant 5 yr ago was admitted to our hospital with a subcutaneous mass on the third finger of the right hand; the mass had been present for several months. He had been receiving immunosuppressive agents for several years. He underwent excision of the mass, which was followed by aspiration of the wound for bacterial and fungal cultures. Many fungal hyphae were observed on the histology slide treated with periodic acid-Schiff stain. A few white waxy colonies with a woolly texture grew on the Sabouraud dextrose agar at 30°C and changed to dark brown in color. Nucleotide sequencing of internal transcribed spacer regions revealed 100% homology to the Phaeoacremonium aleophilum anamorph and Togninia minima teleomorph (514 bp/514 bp). The patient completely recovered after wide surgical excision. Here, we report the first case of phaeohyphomycosis caused by Phaeoacremonium species in a kidney transplant patient in Korea.","PeriodicalId":17890,"journal":{"name":"Korean Journal of Laboratory Medicine","volume":"31 3","pages":"201-4"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3343/kjlm.2011.31.3.201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30022971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Heterogeneous spectrum of CFTR gene mutations in Korean patients with cystic fibrosis. 韩国囊性纤维化患者CFTR基因突变的异质性谱

Korean Journal of Laboratory Medicine Pub Date : 2011-07-01 Epub Date: 2011-06-28 DOI: 10.3343/kjlm.2011.31.3.219

Haiyoung Jung, Chang-Seok Ki, Won-Jung Koh, Kang-Mo Ahn, Sang-Il Lee, Jeong-Ho Kim, Jae Sung Ko, Jeong Kee Seo, Seung-Ick Cha, Eun-Sil Lee, Jong-Won Kim

{"title":"Heterogeneous spectrum of CFTR gene mutations in Korean patients with cystic fibrosis.","authors":"Haiyoung Jung, Chang-Seok Ki, Won-Jung Koh, Kang-Mo Ahn, Sang-Il Lee, Jeong-Ho Kim, Jae Sung Ko, Jeong Kee Seo, Seung-Ick Cha, Eun-Sil Lee, Jong-Won Kim","doi":"10.3343/kjlm.2011.31.3.219","DOIUrl":"https://doi.org/10.3343/kjlm.2011.31.3.219","url":null,"abstract":"Background: Cystic fibrosis (CF) is one of the most common hereditary disorders among Caucasians. The most common mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene have been well established among Caucasian populations. In Koreans, however, there are very few cases of genetically confirmed CF thus far, and the spectrum of mutations seems quite different from that observed in Caucasians.Methods: In the present study, we describe the cases of 2 Korean CF patients, present sequencing results identifying mutations in their CFTR gene, and summarize the results of CFTR mutational spectrum from previously reported Korean CF patients. The mutations described were identified by performing direct sequencing analysis of the complete coding regions and flanking intronic sequences of the CFTR gene, followed by multiplex ligation-dependent probe amplification (MLPA) analysis in order to detect gene deletions or duplications that could not be identified by a direct sequencing method.Results: Three CFTR mutations were identified in the 2 patients, including p.Q98R, c.2052delA, and c.579+5G>A. In an analysis of 9 Korean CF patients that included the 2 patients presented in this study, p.Q98R mutation was the only recurrently observed mutation with a frequency of 18.8% (3/16 alleles). Furthermore, only one of the mutations (c.3272-26A>G) was found among the 32 common mutations in the screening panel for Caucasians from the Cystic Fibrosis Mutation Database.Conclusions: Sequencing of the entire CFTR gene followed by MLPA analysis, rather than using the targeted sequencing-based screening panel for mutations commonly found in Caucasian populations, is recommended for genetic analysis of Korean CF patients.","PeriodicalId":17890,"journal":{"name":"Korean Journal of Laboratory Medicine","volume":"31 3","pages":"219-24"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3343/kjlm.2011.31.3.219","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30022974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Leptin:adiponectin ratio and metabolic syndrome in the general Japanese population. 日本普通人群瘦素:脂联素比率与代谢综合征。

Korean Journal of Laboratory Medicine Pub Date : 2011-07-01 Epub Date: 2011-06-28 DOI: 10.3343/kjlm.2011.31.3.162

Kazuhiko Kotani, Naoki Sakane

{"title":"Leptin:adiponectin ratio and metabolic syndrome in the general Japanese population.","authors":"Kazuhiko Kotani, Naoki Sakane","doi":"10.3343/kjlm.2011.31.3.162","DOIUrl":"https://doi.org/10.3343/kjlm.2011.31.3.162","url":null,"abstract":"Background: Circulating leptin:adiponectin ratio (L:A) is a potential surrogate marker for cardiometabolic diseases; however, the relationship of the L:A with the occurrence of metabolic syndrome (MetS) has not yet been fully explored in the general Japanese population.Methods: We enrolled 678 Japanese subjects (208 men and 470 women, mean age: 58.8±14.4 [SD] yr; mean body mass index: 23.6±3.3 kg/m(2)) in this study, and determined their MetS status by using the National Cholesterol Education Program-Adult Treatment Panel (NCEP-ATP) recommendations with minor modifications for the Japanese population. Biochemical markers such as leptin and adiponectin present in blood were measured. The statistical analyses performed were gender-based.Results: A in subjects with MetS was significantly higher than that in subjects without MetS, regardless of gender. The L:A also showed a significant and gradual increase corresponding to the increase in the number of components of MetS present in both the genders (trend P<0.01). The cut-off level of the L:A to detect MetS was 0.59 (sensitivity: 0.72, specificity: 0.70) in men and 1.04 (sensitivity: 0.72, specificity: 0.69) in women.Conclusions: These results suggest that the L:A can serve as a clinically useful marker for detecting MetS characteristics in the general Japanese population. The clinical application of this laboratory index for detecting MetS should be assessed in future studies.","PeriodicalId":17890,"journal":{"name":"Korean Journal of Laboratory Medicine","volume":"31 3","pages":"162-6"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3343/kjlm.2011.31.3.162","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30023065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Two evaluation budgets for the measurement uncertainty of glucose in clinical chemistry. 临床化学中葡萄糖测量不确定度的两种评定预算。

Korean Journal of Laboratory Medicine Pub Date : 2011-07-01 Epub Date: 2011-06-28 DOI: 10.3343/kjlm.2011.31.3.167

Hui Chen, Ling Zhang, Xiaoyun Bi, Xiaoling Deng

{"title":"Two evaluation budgets for the measurement uncertainty of glucose in clinical chemistry.","authors":"Hui Chen, Ling Zhang, Xiaoyun Bi, Xiaoling Deng","doi":"10.3343/kjlm.2011.31.3.167","DOIUrl":"https://doi.org/10.3343/kjlm.2011.31.3.167","url":null,"abstract":"Background: Measurement uncertainty characterizes the dispersion of the quantity values attributed to a measurand. Although this concept was introduced to medical laboratories some years ago, not all medical researchers are familiar with it. Therefore, the evaluation and expression of measurement uncertainty must be highlighted using a practical example.Methods: In accordance with the procedure for evaluating and expressing uncertainty, provided by the Joint Committee for Guides in Metrology (JCGM), we used plasma glucose (Glu) as an example and defined it as the measurand. We then analyzed the main sources of uncertainty, evaluated each component of uncertainty, and calculated the combined uncertainty and expanded uncertainty with 2 budgets for single measurements and continuous monitoring, respectively.Results: During the measurement of Glu, the main sources of uncertainty included imprecision, within-subject biological variance (BV(w)), calibrator uncertainty, and systematic bias. We evaluated the uncertainty of each component to be 1.26%, 1.91%, 5.70%, 0.42%, and -2.87% for within-run imprecision, between-day imprecision, BV(w), calibrator uncertainty, and systematic bias, respectively. For a single specimen, the expanded uncertainty was 7.38% or 6.1±0.45 mmol/L (κ=2); in continuous monitoring of Glu, the expanded uncertainty was 13.58% or 6.1±0.83 mmol/L (κ=2).Conclusions: We have demonstrated the overall procedure for evaluating and reporting uncertainty with 2 different budgets. The uncertainty is not only related to the medical laboratory in which the measurement is undertaken, but is also associated with the calibrator uncertainty and the biological variation of the subject. Therefore, it is helpful in explaining the accuracy of test results.","PeriodicalId":17890,"journal":{"name":"Korean Journal of Laboratory Medicine","volume":"31 3","pages":"167-71"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3343/kjlm.2011.31.3.167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30023066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Emergence of Clostridium difficile ribotype 027 in Korea. 艰难梭菌027型核型在韩国的出现。

Korean Journal of Laboratory Medicine Pub Date : 2011-07-01 Epub Date: 2011-06-28 DOI: 10.3343/kjlm.2011.31.3.191

Heejung Kim, Yangsoon Lee, Hee-Won Moon, Chae Seung Lim, Kyungwon Lee, Yunsop Chong

{"title":"Emergence of Clostridium difficile ribotype 027 in Korea.","authors":"Heejung Kim, Yangsoon Lee, Hee-Won Moon, Chae Seung Lim, Kyungwon Lee, Yunsop Chong","doi":"10.3343/kjlm.2011.31.3.191","DOIUrl":"https://doi.org/10.3343/kjlm.2011.31.3.191","url":null,"abstract":"Background: Clostridium difficile infection (CDI) has markedly risen and is associated with hypervirulent ribotype 027 outbreaks in North America and Europe since 2003. The aims of this study were to determine the prevalence of ribotype 027 among C. difficile isolates in Korea, to characterize the ribotype 027 isolates, and to determine the clinical severity of CDI in patients infected with these isolates.Methods: A total of 1,251 isolates of C. difficile recovered from stool specimens of suspected CDI patients at two tertiary-care hospitals and one commercial laboratory between 2002 and 2009. Genes for toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA and cdtB) were detected by PCR. Mutation in the tcdC gene was detected by sequencing after PCR amplification. For molecular genotyping, we performed PCR-ribotyping, pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat analysis (MLVA). Minimum inhibitory concentrations of moxifloxacin were determined using Etest strips (AB bioMérieux, Sweden).Results: We identified 7 isolates as ribotype 027. These isolates had the same tcdC mutation as the epidemic strain, and 6 of them were resistant to moxifloxacin. The isolates were categorized into 3 different PFGE types and 7 different MLVA types. All the 7 cases had occurred sporadically.Conclusions: C. difficile ribotype 027 is uncommon, but it has emerged in Korea. The spread of this ribotype should be closely monitored in order to avoid an outbreak of CDI in Korea.","PeriodicalId":17890,"journal":{"name":"Korean Journal of Laboratory Medicine","volume":"31 3","pages":"191-6"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3343/kjlm.2011.31.3.191","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30023070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Mycobacterial infection after intravesical bacillus Calmette-Guërin treatment for bladder cancer: a case report. 膀胱内芽孢杆菌Calmette-Guërin治疗膀胱癌后分枝杆菌感染1例。

Korean Journal of Laboratory Medicine Pub Date : 2011-07-01 Epub Date: 2011-06-28 DOI: 10.3343/kjlm.2011.31.3.197

Chang-Hun Park, Mi Ae Jang, Yoon Hee Ahn, Yu-Yean Hwang, Chang-Seok Ki, Nam Yong Lee

{"title":"Mycobacterial infection after intravesical bacillus Calmette-Guërin treatment for bladder cancer: a case report.","authors":"Chang-Hun Park, Mi Ae Jang, Yoon Hee Ahn, Yu-Yean Hwang, Chang-Seok Ki, Nam Yong Lee","doi":"10.3343/kjlm.2011.31.3.197","DOIUrl":"https://doi.org/10.3343/kjlm.2011.31.3.197","url":null,"abstract":"Bacillus Calmette-Guërin (BCG) has been traditionally used as a vaccine against tuberculosis. Further, intravesical administration of BCG has been shown to be effective in treating bladder cancer. Although BCG contains a live attenuated strain of Mycobacterium bovis, complications such as M. bovis BCG infection caused by BCG administration are extremely rare. Here, we report a case of BCG infection occurring after intravesical BCG therapy. A 67-yr-old man presented with azotemia and weight loss. He had been diagnosed with bladder cancer 4 yr back, and had undergone transurethral resection of the bladder tumor and intravesical BCG (Tice strain) therapy at that time. An acid-fast bacterial strain was isolated from his urine sample. We did not detect Mycobacterium tuberculosis protein 64 (MPT-64) antigen in the isolates obtained from his sample, and multiplex PCR and PCR-reverse blot hybridization assay indicated that the isolate was a member of the M. tuberculosis complex, but was not M. tuberculosis. Finally, sequence analysis of 16S ribosomal RNA and DNA gyrase, subunit B (gyrB) suggested that the organism was M. bovis or M. bovis BCG. Although we could not confirm that M. bovis BCG was the causative agent, the results of the 3 molecular methods and the MPT-64 antigen assay suggest this finding. This is an important finding, especially because M. bovis BCG cannot be identified using common commercial molecular genetics tools.","PeriodicalId":17890,"journal":{"name":"Korean Journal of Laboratory Medicine","volume":"31 3","pages":"197-200"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3343/kjlm.2011.31.3.197","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30022970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells. 富血小板血浆中生长因子浓度的个体差异及其对人间充质干细胞的影响

Korean Journal of Laboratory Medicine Pub Date : 2011-07-01 Epub Date: 2011-06-28 DOI: 10.3343/kjlm.2011.31.3.212

Hee Soon Cho, In Hwan Song, So-Young Park, Min Cheol Sung, Myun-Whan Ahn, Kyung Eun Song

{"title":"Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells.","authors":"Hee Soon Cho, In Hwan Song, So-Young Park, Min Cheol Sung, Myun-Whan Ahn, Kyung Eun Song","doi":"10.3343/kjlm.2011.31.3.212","DOIUrl":"https://doi.org/10.3343/kjlm.2011.31.3.212","url":null,"abstract":"Background: The objective of this study was to explore whether individual variations in the concentration of growth factors (GFs) influence the biologic effects of platelet-rich plasma (PRP) on human mesenchymal stem cells (HMSCs).Methods: The concentrations of 7 representative GFs in activated PRP (aPRP) were measured using ELISA. The effects of PRP on the proliferation and alkaline phosphatase (ALP) activity of HMSCs were examined using several concentrations of aPRP from 3 donors; the relationships between the GF levels and these biologic effects were then evaluated using 10% aPRP from 5 subgroups derived from 39 total donors. HMSCs were cultured in DMEM with the addition of aPRP for 4 or 12 days; then, DNA content and ALP activity were measured.Results: The quantity of DNA increased significantly at a 10% concentration of aPRP, but the ALP activity was suppressed at this concentration of aPRP. The GF concentrations varied among donors, and 5 subgroups of characteristic GF release patterns were identified via cluster analysis. DNA levels differed significantly between groups and tended to be higher in groups with higher concentrations of transforming growth factor-beta1 (TGF-β1) and platelet-derived growth factors (PDGFs). DNA quantity was positively correlated with TGF-β1 concentration, and was negatively correlated with donor age. ALP activity was negatively correlated with PDGF-BB concentration.Conclusions: The varying GF concentrations may result in different biologic effects; thus, individual differences in GF levels should be considered for reliable interpretation of the biologic functions and standardized application of PRP.","PeriodicalId":17890,"journal":{"name":"Korean Journal of Laboratory Medicine","volume":"31 3","pages":"212-8"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3343/kjlm.2011.31.3.212","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30022973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 118

Reliable, accurate determination of the leukocyte differential of leukopenic samples by using Hematoflow method. 用血流法可靠、准确地测定白细胞减少样品的白细胞差异。

Korean Journal of Laboratory Medicine Pub Date : 2011-07-01 Epub Date: 2011-06-28 DOI: 10.3343/kjlm.2011.31.3.131

Yongjun Jo, Soo Hwa Kim, Kwangsang Koh, Jongmoon Park, Yang Bo Shim, Jihyang Lim, Yonggoo Kim, Yeon-Joon Park, Kyungja Han

{"title":"Reliable, accurate determination of the leukocyte differential of leukopenic samples by using Hematoflow method.","authors":"Yongjun Jo, Soo Hwa Kim, Kwangsang Koh, Jongmoon Park, Yang Bo Shim, Jihyang Lim, Yonggoo Kim, Yeon-Joon Park, Kyungja Han","doi":"10.3343/kjlm.2011.31.3.131","DOIUrl":"https://doi.org/10.3343/kjlm.2011.31.3.131","url":null,"abstract":"Background: Hematology analyzers may ineffectively recognize abnormal cells, and manual differential counts may be imprecise for leukopenic samples. We evaluated the efficacy of the Hematoflow method for determining the leukocyte differential in leukopenic samples and compared this method with the manual differential method.Methods: We selected 249 blood samples from 167 patients with leukopenia (WBC counts, 500-2,000/µL) for analysis in this study. The EDTA-anticoagulated blood samples were analyzed using an automatic blood cell counter (DxH800; Beckman Coulter, USA) and flow cytometry (FC 500; Beckman Coulter) by using Cytodiff reagent and analysis software (Beckman Coulter). Hematoflow results were selected or calculated from DxH800 and Cytodiff results. Two trained pathologists performed a manual differential count by counting 50-100 cells.Results: The precision of the Hematoflow method was superior to that of the manual method in counting 5 leukocyte subpopulations, immature granulocytes (IGs), and blasts. Blasts were detected in all 45 cases (100%) by Hematoflow. The correlation of the Cytodiff blast count to the reference count was high (r = 0.8325). For all other cell populations, the correlation of the Hematoflow results with the reference count was stronger than that of the other manual counts with the reference count.Conclusions: The Hematoflow differential counting method is more reproducible and sensitive than manual counting, and is relatively easy to perform. In particular, this method detected leukemic blasts more sensitively than manual differential counts. The Hematoflow method is a very useful supplement to automated cell counting.","PeriodicalId":17890,"journal":{"name":"Korean Journal of Laboratory Medicine","volume":"31 3","pages":"131-7"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3343/kjlm.2011.31.3.131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30024247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Microangiopathic hemolytic anemia as the first manifestation of metastatic signet ring cell carcinoma of unknown origin: a case report and review of literature. 微血管病溶血性贫血为转移性不明来源印戒细胞癌的第一表现:1例报告及文献复习。

Korean Journal of Laboratory Medicine Pub Date : 2011-07-01 Epub Date: 2011-06-28 DOI: 10.3343/kjlm.2011.31.3.157

Sang-Yong Shin, Hyosoon Park, Seoung Wan Chae, Hee-Yeon Woo

{"title":"Microangiopathic hemolytic anemia as the first manifestation of metastatic signet ring cell carcinoma of unknown origin: a case report and review of literature.","authors":"Sang-Yong Shin, Hyosoon Park, Seoung Wan Chae, Hee-Yeon Woo","doi":"10.3343/kjlm.2011.31.3.157","DOIUrl":"https://doi.org/10.3343/kjlm.2011.31.3.157","url":null,"abstract":"Microangiopathic hemolytic anemia (MAHA) occurs occasionally as a paraneoplastic syndrome in some solid tumors, but MAHA accompanied by signet ring cell carcinoma of an unknown origin is very rare. In this study, we present the case of an 80-yr-old man who was admitted to the hospital because of a 1-month history of lower back pain and dyspnea. He was diagnosed with MAHA on the basis of the laboratory findings that revealed anemia with schistocytes, decreased haptoglobin levels, and a negative direct Coombs' test. Bone marrow examination, which was performed because of the progression of anemia, revealed bone marrow metastases of signet ring cell carcinoma with extensive bone marrow necrosis. However, the primary origin of this signet ring cell carcinoma was not found. When the cause of progressive MAHA is unknown, the possibility of cancer-associated MAHA must be excluded by performing additional tumor workup, including the detection of tumor markers, gastric and colorectal endoscopic examinations, bone marrow examinations, and positron emission tomography-computed tomography or bone scans.","PeriodicalId":17890,"journal":{"name":"Korean Journal of Laboratory Medicine","volume":"31 3","pages":"157-61"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3343/kjlm.2011.31.3.157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30023064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19