Kisaengch'unghak chapchi. The Korean journal of parasitology最新文献

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Sarcocystis morae (Apicomplexa) in Fallow Deer (Dama dama) from Spain: Ultrastructure and New Host Record. 西班牙小鹿(Dama Dama)的摩氏肉囊菌(顶复合体):超微结构和新宿主记录。
Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2019-10-01 DOI: 10.1645/19-56
Guillermo E Delgado de Las Cuevas, P. Prakas, Ž. Strazdaitė-Žielienė, M. Martínez-González, E. Rudaitytė-Lukošienė, D. Butkauskas, E. Servienė, M. Habela, R. Calero-Bernal
{"title":"Sarcocystis morae (Apicomplexa) in Fallow Deer (Dama dama) from Spain: Ultrastructure and New Host Record.","authors":"Guillermo E Delgado de Las Cuevas, P. Prakas, Ž. Strazdaitė-Žielienė, M. Martínez-González, E. Rudaitytė-Lukošienė, D. Butkauskas, E. Servienė, M. Habela, R. Calero-Bernal","doi":"10.1645/19-56","DOIUrl":"https://doi.org/10.1645/19-56","url":null,"abstract":"Members of the genus Sarcocystis are frequently found infecting members of the family Cervidae. Although Sarcocystis species are generally host specific for their intermediate hosts, species in cervids appear to be less host specific. Here, we report fallow deer (Dama dama) as a new host for Sarcocystis morae, originally described from the red deer (Cervus elaphus). Tongues of 69 legally hunted animals in Spain were tested for sarcocysts, and the species were characterized by light microscopy, ultrastructurally and molecularly. Sarcocysts were identified in 66.7% of D. dama. Sarcocysts had thin (<2 μm thick) cyst wall with hair-like villar protrusions bifurcated at their tips resembling type 8a. Genetic sequences obtained for 18S rRNA and COI reached 99.6-100% and 97.9-98.7% similarity, respectively, to those of S. morae from the red deer. The present study provides new data concerning lower level of host specificity within Sarcocystis genus for cervids.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"477 1","pages":"813-815"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79943227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Subcutaneous Sparganosis on Abdomen Mimicking Multiple Lipomas 腹部皮下spargosis模拟多发性脂肪瘤
Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2019-10-01 DOI: 10.3347/kjp.2019.57.5.513
Seung Ki Ahn, Hwanjun Choi, Jun Hyuk Kim
{"title":"Subcutaneous Sparganosis on Abdomen Mimicking Multiple Lipomas","authors":"Seung Ki Ahn, Hwanjun Choi, Jun Hyuk Kim","doi":"10.3347/kjp.2019.57.5.513","DOIUrl":"https://doi.org/10.3347/kjp.2019.57.5.513","url":null,"abstract":"Human sparganosis is a food-borne zoonosis mainly caused by the plerocercoid belonging to the genus Spirometra. The most common clinical sign of sparganosis is a subcutaneous mass in the trunk including abdominal or chest wall. The mass may be mistaken for a malignant tumor, thereby causing difficulty in terms of diagnosis and treatment. A 66-year-old woman visited our clinic for the removal of a lipoma-like mass. It was movable, hard, and painless. We identified 2 white mass, measuring 0.2×4 cm and 0.2×1 cm. Pathologic findings indicated the white mass was a sparganum. She recalled having eaten a raw frog approximately 60 years before. A 35-year-old who lived North Korea was also presented to our clinic with an asymptomatic nodule on her abdomen. Intraoperatively, we found sparganum approximately 24 cm size. Subcutaneous masses are associated with clinical signs of inflammation or they may mimic a soft tissue neoplasm. While the incidence rate of sparganosis has decreased with economic development and advancements in sanitation, surgeons still encounter patients with sparganosis in the clinical setting. Therefore, a careful history is required in order to diagnose sparganosis.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"85 1","pages":"513 - 516"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90927118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Mitochondrial DNA Sequence Variability of Spirometra Species in Asian Countries 亚洲国家螺蝇种线粒体DNA序列变异
Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2019-10-01 DOI: 10.3347/kjp.2019.57.5.481
H. Jeon, K. Eom
{"title":"Mitochondrial DNA Sequence Variability of Spirometra Species in Asian Countries","authors":"H. Jeon, K. Eom","doi":"10.3347/kjp.2019.57.5.481","DOIUrl":"https://doi.org/10.3347/kjp.2019.57.5.481","url":null,"abstract":"Mitochondrial DNA sequence variability of Spirometra erinaceieuropaei in GenBank was observed by reinvestigation of mitochondrial cox1 and cytb sequences. The DNA sequences were analyzed in this study, comprising complete DNA sequences of cox1 (n=239) and cytb (n=213) genes. The 10 complete mitochondrial DNA sequences of Spirometra species were compared with those of Korea, China and Japan. The sequences were analyzed for nucleotide composition, conserved sites, variable sites, singleton sites and parsimony-informative sites. Phylogenetic analyses was done using neighbor joining, maximum parsimony, Bayesian inference and maximum-likelihood on cox1 and cytb sequences of Spirometra species. These polymorphic sites identified 148 (cox1) and 83 (cytb) haplotypes within 239 and 213 isolates from 3 Asian countries. Phylogenetic tree topologies were presented high-level confidence values for the 2 major branches of 2 Spirometra species containing S. erinaceieuropaei and S. decipiens, and S. decipiens sub-clades including all sequences registered as S. erinaceieuropaei in cox1 and cytb genes. These results indicated that mitochondrial haplotypes of S. erinaceieuropaei and S. decipiens were found in the 3 Asian countries.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"26 1","pages":"481 - 487"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85621162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
The Proteocephalus Species-Aggregate in Freshwater Centrarchid and Percid Fishes of the Nearctic Region (North America). 北美新北极地区淡水中央科和Percid鱼类的变形头目种集。
Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2019-10-01 DOI: 10.1645/19-89
T. Scholz, A. Choudhury, L. Uhrová, J. Brabec
{"title":"The Proteocephalus Species-Aggregate in Freshwater Centrarchid and Percid Fishes of the Nearctic Region (North America).","authors":"T. Scholz, A. Choudhury, L. Uhrová, J. Brabec","doi":"10.1645/19-89","DOIUrl":"https://doi.org/10.1645/19-89","url":null,"abstract":"In the present paper, species of the Proteocephalus-aggregate de Chambrier, Zehnder, Vaucher, and Mariaux, 2004 (Cestoda: Proteocephalidae) reported from centrarchid and percid fishes in North America are reviewed, and their taxonomic status is critically assessed based on a study of type specimens and new material from Canada and the United States. The following 3 species, supposedly strictly specific to their fish definitive hosts, are recognized as valid: (1) Proteocephalus fluviatilisBangham, 1925 (new synonyms Proteocephalus osburniBangham, 1925 and Proteocephalus microcephalusHaderlie, 1953; Proteocephalus 'robustus' nomen nudum) from the smallmouth and largemouth bass, Micropterus dolomieu (Lacépède) (type host) and Micropterus salmoides (Lacépède) (both Centrarchidae); (2) Proteocephalus luciopercaeWardle, 1932 (new synonym Proteocephalus stizostethiHunter and Bangham, 1933) from the walleye, Sander vitreus (Mitchill) (type host), and sauger, Sander canadensis (Griffith et Smith) (Percidae); and (3) Proteocephalus pearseiLa Rue, 1919, a parasite of the yellow perch, Perca flavescens Mitchill (Percidae). All species are illustrated based on new, properly heat-fixed material. Scanning electron micrographs of the scoleces of percid tapeworms P. luciopercae and P. pearsei, as well as the bass tapeworms P. fluviatilis and Proteocephalus ambloplitis (Leidy, 1887), the latter of which does not belong to this Proteocephalus-aggregate, are provided for the first time together with a simple key to species identification of proteocephalids from centrarchiform and perciform teleost fishes.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"1 1","pages":"798-812"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82180485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013–2016 2013-2016年韩国急性腹泻患者小隐孢子虫和十二指肠贾第虫的分子流行及基因型分析
Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2019-10-01 DOI: 10.3347/kjp.2019.57.5.531
D. Ma, Myoung-Ro Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang-Eun Lee
{"title":"Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013–2016","authors":"D. Ma, Myoung-Ro Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang-Eun Lee","doi":"10.3347/kjp.2019.57.5.531","DOIUrl":"https://doi.org/10.3347/kjp.2019.57.5.531","url":null,"abstract":"Cryptosporidium parvum and Giardia duodenalis are the main diarrhea-causing parasitic pathogens; however, their prevalence in Korea is unknown. Here, we conducted a survey to determine the prevalence and genotype distribution of these 2 pathogens causing acute diarrhea in 8,571 patients hospitalized in 17 Regional Institute of Health Environment sites in Korea, during 2013–2016. C. parvum and G. duodenalis were detected and genotyped by nested PCR, and the isolate were molecularly characterized by sequencing the glycoprotein 60 (Gp60) and β-giardin genes, respectively. The overall prevalence of C. parvum and G. duodenalis was 0.37% (n=32) and 0.55% (n=47), respectively, and both pathogens were more prevalent in children under 9 years old. Molecular epidemiological analysis showed that the C. parvum isolates belonged to the IIa family and were subtyped as IIaA13G2R1, IIaA14G2R1, IIaA15G2R1, and IIaA18G3R1. Analysis of the β-giardin gene fragment from G. duodenalis showed that all positive strains belong to assemblage A. This is the first report on the molecular epidemiology and subtyping of C. parvum and G. duodenalis in such a large number of diarrheal patients in Korea. These results highlight the need for continuous monitoring of these zoonotic pathogens and provide a basis for implementing control and prevention strategies. Further, the results might be useful for epidemiological investigation of the source of outbreak.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"23 1","pages":"531 - 536"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79450037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
A New Eimerian (Apicomplexa: Eimeriidae) from the Barn Swallow, Hirundo rustica (Aves: Passeriformes: Hirundinidae), in Southeastern Oklahoma: The Fourth Eimerian Species from New World Passeriformes.
Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2019-10-01 DOI: 10.1645/19-105
C. T. McAllister, J. Hnida
{"title":"A New Eimerian (Apicomplexa: Eimeriidae) from the Barn Swallow, Hirundo rustica (Aves: Passeriformes: Hirundinidae), in Southeastern Oklahoma: The Fourth Eimerian Species from New World Passeriformes.","authors":"C. T. McAllister, J. Hnida","doi":"10.1645/19-105","DOIUrl":"https://doi.org/10.1645/19-105","url":null,"abstract":"Barn swallows (Hirundo rustica) are the most widespread swallow species in the world. However, little is known about the coccidian parasites of H. rustica. Feces from a single H. rustica nesting in McCurtain County, Oklahoma, were collected in May 2018 and examined for coccidia; the swallow was found to be passing a new species of Eimeria. Oocysts of Eimeria hochatownensis n. sp. are ellipsoidal with a smooth bi-layered wall, measure (L × W) 25.5 × 15.2 μm, and have a length/width (L/W) ratio of 1.7; a micropyle and oocyst residuum are absent, but polar granule(s) are present. Sporocysts are ellipsoidal and measure 12.8 × 6.5 μm, L/W 2.0; a nipple-like Stieda body is present, but sub-Stieda bodies and para-Stieda bodies are absent. The sporocyst residuum is composed of a compact spheroid with a dense, irregular mass of finer granules lying between and dispersed among the sporozoites. This is the first eimerian reported from H. rustica and the family Hirundinidae, and only the fourth Eimeria spp. known from passerine birds of the New World.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"178 1","pages":"693-696"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73204373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Effects of River Hydrology and Physicochemistry on Anchovy Abundance and Cymothoid Isopod Parasitism. 河流水文和物理化学对凤尾鱼丰度和鲤形等足类寄生的影响。
Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2019-10-01 DOI: 10.1645/19-63
Bryan N MacNeill, M. Lajeunesse
{"title":"Effects of River Hydrology and Physicochemistry on Anchovy Abundance and Cymothoid Isopod Parasitism.","authors":"Bryan N MacNeill, M. Lajeunesse","doi":"10.1645/19-63","DOIUrl":"https://doi.org/10.1645/19-63","url":null,"abstract":"The flow regime of a river is an important driver of many ecosystem components. However, few studies explore how differences in flow rates and water chemistry can influence communities of parasites and their hosts. Here, we investigate the impact of dissolved oxygen, pH, salinity, water temperature, and river flow on the abundance and prevalence of cymothoid isopod parasitism (Lironeca ovalis) of the Bay Anchovy (Anchoa mitchilli) in the Alafia and Hillsborough rivers of Tampa Bay (Florida). We also explore seasonality by comparing monthly samples preserved throughout 2005-2007. Although both the Alafia and Hillsborough rivers had similar average water temperatures and salinity, and similar wet and dry season cycles, the upstream damming of the Hillsborough River had numerous negative effects on water flow rate, dissolved oxygen content, and acidity. This disruption in water quality corresponded with a lower abundance of anchovy hosts, fewer free-swimming cymothoids, and low prevalence of anchovy parasitism. Anchovies were much more abundant in the Alafia River, but flow negatively affected abundance-a negative effect that could be mitigated by positive changes in water temperature, salinity, and pH. Flow rates also negatively affected free-swimming cymothoid abundance; however, water flow was less important in predicting their parasitism of anchovies. In Alafia, fewer anchovies were parasitized when dissolved oxygen was high and water acidity was low, but more were parasitized during the wet season. These findings corroborate predictions that flow can moderate habitat stability and complexity which, in turn, can impact opportunities for parasitism of host communities.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"38 1","pages":"760-768"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74005158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Prevalence of Intestinal Helminth Infections in Dogs and Two Species of Wild Animals from Samarkand Region of Uzbekistan 乌兹别克斯坦撒马尔罕地区犬类和两种野生动物肠道蠕虫感染流行病学研究
Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2019-10-01 DOI: 10.3347/kjp.2019.57.5.549
T. Yong, Kyu-Jae Lee, M. Shin, H. Yu, U. Suvonkulov, Turycin Bladimir Sergeevich, A. Shamsiev, G. Park
{"title":"Prevalence of Intestinal Helminth Infections in Dogs and Two Species of Wild Animals from Samarkand Region of Uzbekistan","authors":"T. Yong, Kyu-Jae Lee, M. Shin, H. Yu, U. Suvonkulov, Turycin Bladimir Sergeevich, A. Shamsiev, G. Park","doi":"10.3347/kjp.2019.57.5.549","DOIUrl":"https://doi.org/10.3347/kjp.2019.57.5.549","url":null,"abstract":"This study aimed to determine the prevalence of intestinal helminth parasitic infections and associated risk factors for the human infection among the people of Samarkand, Uzbekistan. Infection status of helminths including Echinococcus granulosus was surveyed in domestic and wild animals from 4 sites in the Samarkand region, Uzbekistan during 2015–2018. Fecal samples of each animal were examined with the formalin-ether sedimentation technique and the recovery of intestinal helminths was performed with naked eyes and a stereomicroscope in total 1,761 animals (1,755 dogs, 1 golden jackal, and 5 Corsac foxes). Total 658 adult worms of E. granulosus were detected in 28 (1.6%) dogs and 1 (100%) golden jackal. More than 6 species of helminths, i.e., Taenia hydatigena, Dipylidium caninum, Diplopylidium nolleri, Mesocestoides lineatus, Toxocara canis, and Trichuris vulpis, were found from 18 (1.0%) dogs. Six (T. hydatigena, Toxascaris leonina, Alaria alata, Uncinaria stenocephala, D. caninum, and M. lineatus) and 2 (D. nolleri and M. lineatus) species of helminths were also detected from 5 Corsac foxes and 1 golden jackal, respectively. Taeniid eggs were found in 2 (20%) out of 10 soil samples. In the present study, it was confirmed that the prevalences of helminths including E. granulosus are not so high in domestic and wild animals. Nevertheless, the awareness on the zoonotic helminth infections should be continuously maintained in Uzbekistan for the prevention of human infection.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"15 9-10","pages":"549 - 552"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91497309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Two New Species of Haematoloechus (Digenea: Plagiorchidae) Parasitizing Rana brownorum (Amphibia: Ranidae) from Southeast Mexico. 墨西哥东南部寄生褐蛙(两栖目:蛙科)的血蝗二新种。
Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2019-10-01 DOI: 10.1645/18-169
Y. Velázquez-Urrieta, A. Oceguera-Figueroa, V. León-Règagnon
{"title":"Two New Species of Haematoloechus (Digenea: Plagiorchidae) Parasitizing Rana brownorum (Amphibia: Ranidae) from Southeast Mexico.","authors":"Y. Velázquez-Urrieta, A. Oceguera-Figueroa, V. León-Règagnon","doi":"10.1645/18-169","DOIUrl":"https://doi.org/10.1645/18-169","url":null,"abstract":"In an ongoing investigation on the helminths of amphibians in southeastern Mexico, specimens of 2 undescribed species of Haematoloechus were collected from Rana brownorum. Haematoloechus ceciliae n. sp. is morphologically most similar to Haematoloechus meridionalis, but differs in the shape of the oral sucker, in the nature of the acetabulum, and in the distribution of the glandular cells in the pharyngeal region; Haematoloechus celestunensis n. sp. closely resembles Haematoloechus floedae, but differs in the form and size of the testes and measurements of acetabulum. COI and 28S DNA sequences of both new species show high divergence compared to other species of the genus. In the phylogenetic trees, H. ceciliae appears most closely related to Haematoloechus danbrooksi and H. celestunensis to Haematoloechus veracruzanus.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"1 1","pages":"724-732"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77974914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Identification of a Short DNA Bar Code in the 18S rDNA for Improved Differentiation of Common Eimeria Species Infecting Chickens. 鸡常见艾美耳球虫的18S rDNA短条形码鉴定。
Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2019-10-01 DOI: 10.1645/19-34
G. Albanese, Dong‐Hun Lee, Adrea E Mueller, B. Jordan
{"title":"Identification of a Short DNA Bar Code in the 18S rDNA for Improved Differentiation of Common Eimeria Species Infecting Chickens.","authors":"G. Albanese, Dong‐Hun Lee, Adrea E Mueller, B. Jordan","doi":"10.1645/19-34","DOIUrl":"https://doi.org/10.1645/19-34","url":null,"abstract":"The coccidian species Eimeria is a parasitic protozoan that causes the gastrointestinal disease coccidiosis in numerous vertebrate species. Incidence of the disease in commercial chickens produces drastic economic losses. Traditionally, detection of Eimeria has been performed using classical methods such as observation of oocyst morphology. However, molecular methods to detect and speciate Eimeria are becoming more prevalent. The 18S ribosomal gene, in particular, has been a widely used DNA amplification target for detection of Eimeria. Although the full-length gene is typically used for this purpose, newer research targeting shorter regions of the gene is being performed. This study investigated the suitability of a 120-base pair (bp) DNA bar code within the 18S gene for species differentiation. When comparing sequence variation from the Eimeria species infecting chickens, shortening the 18S gene to the 120-bp highly variable region provided increased species differentiation, while also reducing intraspecies variation. This DNA bar code is useful for distinction of the Eimeria species infecting chickens and should be considered for future molecular detection assays and metagenomic sequencing.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"77 1","pages":"816-820"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72687444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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