Kisaengch'unghak chapchi. The Korean journal of parasitology最新文献_第2页

Echinococcus granulosus Protoscolex DM9 Protein Shows High Potential for Serodiagnosis of Alveolar Echinococcosis 细粒棘球蚴原头节DM9蛋白在肺泡棘球蚴病的血清诊断中具有很高的潜力

Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2022-02-01 DOI: 10.3347/kjp.2022.60.1.25

Jeong-Geun Kim, Xiumin Han, Y. Kong

{"title":"Echinococcus granulosus Protoscolex DM9 Protein Shows High Potential for Serodiagnosis of Alveolar Echinococcosis","authors":"Jeong-Geun Kim, Xiumin Han, Y. Kong","doi":"10.3347/kjp.2022.60.1.25","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.1.25","url":null,"abstract":"Alveolar echinococcosis (AE) caused by infection with E. multilocularis metacestode, represents one of the most fatal helminthic diseases. AE is principally manifested with infiltrative, proliferating hepatic mass, resembling primary hepatocellular carcinoma. Sometimes metastatic lesions are found in nearby or remote tissue. AE diagnosis largely depends on imaging studies, but atypical findings of imaging features frequently require differential diagnosis from other hepatic lesions. Serological tests may provide further evidence, while obtaining reliable AE materials is not easy. In this study, alternative antigens, specific to AE were identified by analyzing E. granulosus protoscolex proteins. An immunoblot analysis of E. granulosus protoscolex showed that a group of low-molecular-weight proteins in the range from 14 kDa to 16 kDa exhibited a sensitive and specific immune response to AE patient sera. Partial purification and proteomic analysis indicated that this protein group contained myosin, tubulin polymerization promoting protein, fatty-acid binding protein, uncharacterized DM9, heat shock protein 90 cochaperone tebp P-23, and antigen S. When the serological applicability of recombinant forms of these proteins was assessed using enzyme-linked immunosorbent assay, DM9 protein (rEgDM9) showed 90.1% sensitivity (73/81 sera tested) and 94.5% specificity (172/181 sera tested), respectively. rEgDM9 showed weak cross-reactions with patient sera from the transitional and chronic stages of cystic echinococcosis (3 to 5 stages). rEgDM9 would serve as a useful alternative antigen for serodiagnosis of both early- and advanced-stage AE cases.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"29 1","pages":"25 - 34"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85237047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introduction of Non-Native Ticks Collected from Fresh Migratory Bird Carcasses on a Stopover Island in the Republic of Korea 在大韩民国的一个中途停留岛上从新鲜候鸟尸体上收集的外来蜱虫的引入

Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2022-02-01 DOI: 10.3347/kjp.2022.60.1.57

Chang-Yong Choi, Heung-Chul Kim, T. Klein, H. Nam, Gi-Chang Bing

{"title":"Introduction of Non-Native Ticks Collected from Fresh Migratory Bird Carcasses on a Stopover Island in the Republic of Korea","authors":"Chang-Yong Choi, Heung-Chul Kim, T. Klein, H. Nam, Gi-Chang Bing","doi":"10.3347/kjp.2022.60.1.57","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.1.57","url":null,"abstract":"When free-ranging birds are accidentally killed or die, there may be greater potential for their associated ticks to detach, seek alternate hosts, and become established. We examined 711 carcasses of 95 avian species for ticks at a stopover island of migratory birds in the Republic of Korea where only Ixodes nipponensis and I. persulcatus were previously reported from local mammals and vegetation. A total of 16 ticks, I. turdus and Haemaphysalis flava, were collected from 8 fresh carcasses belonging to 5 avian species. Despite their known abundance on migratory birds and mainland Korea, these species had not colonized the isolated insular ecosystem possibly due to the low abundance and diversity of local hosts. The results imply that increasing human impact, such as the anthropogenic mortality of migratory birds and the introduction of non-native mammalian hosts, will increase the potential invasion and colonization risk of ticks. This finding also suggests that tick surveillance consisting of fresh carcasses of dead migratory birds may provide additional information, often ignored in surveillance of ticks on live birds, for the potential introduction of non-native ticks and associated pathogens affecting animal and human health.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"2 1","pages":"57 - 63"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78968280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterization of a Peptide Antibody Specific to the Adenylyl Cyclase-Associated Protein of Acanthamoeba castellanii 棘阿米巴腺苷酸环化酶相关蛋白特异性肽抗体的鉴定

Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2022-02-01 DOI: 10.3347/kjp.2022.60.1.7

Min-Jeong Kim, Hae-Ahm Lee, F. Quan, H. Kong, Eun-Kyung Moon

{"title":"Characterization of a Peptide Antibody Specific to the Adenylyl Cyclase-Associated Protein of Acanthamoeba castellanii","authors":"Min-Jeong Kim, Hae-Ahm Lee, F. Quan, H. Kong, Eun-Kyung Moon","doi":"10.3347/kjp.2022.60.1.7","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.1.7","url":null,"abstract":"Acanthamoeba keratitis (AK) is a rare infectious disease and accurate diagnosis has remained arduous as clinical manifestations of AK were similar to keratitis of viral, bacterial, or fungal origins. In this study, we described the production of a polyclonal peptide antibody against the adenylyl cyclase-associated protein (ACAP) of A. castellanii, and evaluated its differential diagnostic potential. Enzyme-linked immunosorbent assay revealed high titers of A. castellanii-specific IgG and IgA antibodies being present in low dilutions of immunized rabbit serum. Western blot analysis revealed that the ACAP antibody specifically interacted with A. castellanii, while not interacting with human corneal epithelial (HCE) cells and other causes of keratitis such as Fusarium solani, Pseudomonas aeruginosa, and Staphylococcus aureus. Immunocytochemistry (ICC) results confirmed the specific detection of trophozoites and cysts of A. castellanii co-cultured with HCE cells. The ACAP antibody also specifically interacted with the trophozoites and cysts of 5 other Acanthamoeba species. These results indicate that the ACAP antibody of A. castellanii can specifically detect multiple AK-causing members belonging to the genus Acanthamoeba and may be useful for differentially diagnosing Acanthamoeba infections.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"91 1","pages":"7 - 14"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85842027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Severe Fever with Thrombocytopenia Syndrome Virus in Ticks in the Republic of Korea 大韩民国蜱中的发热伴血小板减少综合征病毒

Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2022-02-01 DOI: 10.3347/kjp.2022.60.1.65

Jun-Gu Kang, Yoon-Kyoung Cho, Young-Sun Jo, Sun-Woo Han, Jeong-Byoung Chae, Jung-Eun Park, Hyesung Jeong, W. Jheong, J. Chae

{"title":"Severe Fever with Thrombocytopenia Syndrome Virus in Ticks in the Republic of Korea","authors":"Jun-Gu Kang, Yoon-Kyoung Cho, Young-Sun Jo, Sun-Woo Han, Jeong-Byoung Chae, Jung-Eun Park, Hyesung Jeong, W. Jheong, J. Chae","doi":"10.3347/kjp.2022.60.1.65","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.1.65","url":null,"abstract":"Severe fever with thrombocytopenia syndrome virus (SFTSV) is a zoonotic, tick-borne RNA virus of the genus Bandavirus (Family Phenuiviridae), mainly reported in China, Japan, and the Republic of Korea (Korea). For the purpose of this study, a total of 3,898 adult and nymphal ticks of species Haemaphysalis longicornis (94.2%), Haemaphysalis flava (5.0%), Ixodes nipponensis (0.8%), and 1 specimen of Ixodes ovatus, were collected from the Deogyusan National Park, Korea, between April 2016 and June 2018. A single-step reverse transcriptase–nested PCR was performed, targeting the S segment of the SFTSV RNA. Total infection rate (IR) of SFTSV in individual ticks was found to be 6.0%. Based on developmental stages, IR was 5.3% in adults and 6.0% in nymphs. The S segment sequences obtained from PCR were divided into 17 haplotypes. All haplotypes were phylogenetically clustered into clades B-2 and B-3, with 92.7% sequences in B-2 and 7.3% in B-3. These observations indicate that the Korean SFTSV strains were closer to the Japanese than the Chinese strains. Further epidemiological studies are necessary to better understand the characteristics of the Korean SFTSV and its transmission cycle in the ecosystem.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"60 1","pages":"65 - 71"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74383375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Association Between the Prevalence of Schistosomiasis in Elementary School Students and Their Parental Occupation in Sudan 苏丹小学生血吸虫病流行与父母职业的关系

Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2022-02-01 DOI: 10.3347/kjp.2022.60.1.51

Yan Jin, Seungman Cha, Youngjin Kim, H. Hamdan, M. Elhag, H. Ismail, K. Lee, Sung-Tae Hong

{"title":"Association Between the Prevalence of Schistosomiasis in Elementary School Students and Their Parental Occupation in Sudan","authors":"Yan Jin, Seungman Cha, Youngjin Kim, H. Hamdan, M. Elhag, H. Ismail, K. Lee, Sung-Tae Hong","doi":"10.3347/kjp.2022.60.1.51","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.1.51","url":null,"abstract":"Global efforts to identify groups at high risk for schistosomiasis have mainly concentrated on identifying their geographical distribution. Investigations on the socioeconomic characteristics of high-risk groups are relatively scarce. This study aimed to explore the associations between schistosomiasis among students and their parents’ occupations. A nationwide cross-sectional survey was conducted targeting 105,167 students in 1,772 primary schools across Sudan in 2017. From these students, 100,726 urine and 96,634 stool samples were collected to test for Schistosoma haematobium and S. mansoni infection. A multi-level mixed effect analysis was used with age and sex as fixed factors, and school as a random factor. The odd ratios (ORs) of practicing open defecation among farmers’ children were almost 5 times higher than their counterparts whose parents were government officials (OR=4.97, 95% confidence intervals (CIs): 4.57–5.42, P<0.001). The ORs of contacting water bodies for watering livestock among farmers’ children were more than 4 times higher than those of children whose parents were government officials (OR=4.59, 95% CIs: 4.02–5.24, P<0.001). This study shows that schistosomiasis represents a disease of poverty and that farmers’ children constituted a high-risk group.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"16 1","pages":"51 - 56"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87158306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein Sirtinol通过抑制Sirtuin家族蛋白抑制棘阿米巴滋养体增殖和成环

Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2022-02-01 DOI: 10.3347/kjp.2022.60.1.1

So-Young Joo, Ja Moon Aung, M. Shin, Eun-Kyung Moon, H. Kong, Y. Goo, D. Chung, Yeonchul Hong

{"title":"Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein","authors":"So-Young Joo, Ja Moon Aung, M. Shin, Eun-Kyung Moon, H. Kong, Y. Goo, D. Chung, Yeonchul Hong","doi":"10.3347/kjp.2022.60.1.1","DOIUrl":"https://doi.org/10.3347/kjp.2022.60.1.1","url":null,"abstract":"The encystation of Acanthamoeba leads to the development of metabolically inactive and dormant cysts from vegetative trophozoites under unfavorable conditions. These cysts are highly resistant to anti-Acanthamoeba drugs and biocides. Therefore, the inhibition of encystation would be more effective in treating Acanthamoeba infection. In our previous study, a sirtuin family protein—Acanthamoeba silent-information regulator 2-like protein (AcSir2)—was identified, and its expression was discovered to be critical for Acanthamoeba castellanii proliferation and encystation. In this study, to develop Acanthamoeba sirtuin inhibitors, we examine the effects of sirtinol, a sirtuin inhibitor, on trophozoite growth and encystation. Sirtinol inhibited A. castellanii trophozoites proliferation (IC50=61.24 μM). The encystation rate of cells treated with sirtinol significantly decreased to 39.8% (200 μM sirtinol) after 24 hr of incubation compared to controls. In AcSir2-overexpressing cells, the transcriptional level of cyst-specific cysteine protease (CSCP), an Acanthamoeba cysteine protease involved in the encysting process, was 11.6- and 88.6-fold higher at 48 and 72 hr after induction of encystation compared to control. However, sirtinol suppresses CSCP transcription, resulting that the undegraded organelles and large molecules remained in sirtinol-treated cells during encystation. These results indicated that sirtinol sufficiently inhibited trophozoite proliferation and encystation, and can be used to treat Acanthamoeba infections.","PeriodicalId":17889,"journal":{"name":"Kisaengch'unghak chapchi. The Korean journal of parasitology","volume":"36 1","pages":"1 - 6"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80864381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Editorial Acknowledgments (Volume 106, 2020). 编辑致谢(卷106,2020)。

Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2020-11-12 DOI: 10.1645/20-159

R. E. Clopton

引用次数: 0

Cestode Parasites of Armadillos (Mammalia: Xenarthra) from Argentina: Two New Species of Mathevotaenia (Anoplocephalidae) in Tolypeutes matacus. 阿根廷犰狳寄生虫(哺乳纲:Xenarthra):犰狳科Mathevotaenia (anoplocphalidae)二新种。

Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2020-02-01 DOI: 10.1645/19-103

Tatiana A Rios, F. Drago, M. Ezquiaga, A. Abba, G. Navone

引用次数: 0

Atrophic Proventriculitis Associated with Gravid Females of Tetrameres sp. (Nematoda: Tetrameridae) in the Magellanic Penguin (Spheniscus magellanicus) on the Coast of Brazil. 巴西海岸麦哲伦企鹅(Spheniscus magellanicus)四足虫属(线虫纲:四足虫科)妊娠雌性的萎缩性前脑室炎。

Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2020-02-01 DOI: 10.1645/18-105

M. Werneck, H. Jerdy, R. B. Ribeiro, M. Bianchi, L. L. F. Maciel, João Carlos de Aquino Almeida, P. Baldassin, H. Gallo, E. Carvalho

引用次数: 1

Is Allopreening a Stimulus-Driven Defense Against Ectoparasites? 异丙烯化是刺激驱动的对抗体外寄生虫的防御吗?

Kisaengch'unghak chapchi. The Korean journal of parasitology Pub Date : 2020-02-01 DOI: 10.1645/19-104

Graham B. Goodman, Sarah A Conner, Sarah E. Bush, D. Clayton

引用次数: 0