Korean Journal of Applied Entomology最新文献_第9页

Cellular Immune Response of Protaetia brevitarsis seulensis Larvae to Metabolites Produced by Pathogenic and Symbiotic Bacteria 短视原蜂幼虫对病原菌和共生菌代谢物的细胞免疫反应

Korean Journal of Applied Entomology Pub Date : 2018-01-01 DOI: 10.5656/KSAE.2017.12.0.036

Dooseon Hwang, Saeyoull Cho

{"title":"Cellular Immune Response of Protaetia brevitarsis seulensis Larvae to Metabolites Produced by Pathogenic and Symbiotic Bacteria","authors":"Dooseon Hwang, Saeyoull Cho","doi":"10.5656/KSAE.2017.12.0.036","DOIUrl":"https://doi.org/10.5656/KSAE.2017.12.0.036","url":null,"abstract":"Studies of immune responses in insects have focused on mechanisms that interact directly with invading microorganisms. However, few studies have examined the immune response to various metabolites produced by microorganisms after they enter the host. Here, we examined immune responses in Protaetia brevitarsis seulensis larvae induced by metabolites produced by symbiotic and pathogenic bacteria. The two types of bacteria were cultured under the same conditions. The bacteria were then removed and the remaining culture supernatant was injected into the larvae. The larvae injected with culture medium (Ch-medium) from symbiotic bacteria remained relatively healthy and did not develop an immune response, whereas more than 60% of the larvae injected with pathogen culture medium (Ec-medium) died after 150 hours and dark brown patches of melanin were observed at the injection site. This immune response was confirmed by the finding of activated lysosomes in insect granulocytes. More than 50% of lysosomes in larvae injected with pathogen culture medium were strongly stained after 12 h, but less than 5% of those injected with symbiotic culture media were stained. Therefore, it is assumed that symbiotic bacteria produce few (if any) substances that induce host immune responses.","PeriodicalId":17836,"journal":{"name":"Korean Journal of Applied Entomology","volume":"72 1","pages":"25-32"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86133206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

살구나무 해충 Pristiphora apricoti Zinovjev (벌목: 잎벌아목: 잎벌과: 수염잎벌아과)에 대한 보고 关于杏树害虫Pristiphora apricoti Zinovjev的报告

Korean Journal of Applied Entomology Pub Date : 2018-01-01 DOI: 10.5656/KSAE.2018.07.0.022

Choi Jin-Kyung, Lee Jong Wook

引用次数: 0

A Loop-mediated Isothermal Amplification Method for White-backed Planthopper-specific Detection 环介导等温扩增法检测白背飞虱特异性

Korean Journal of Applied Entomology Pub Date : 2018-01-01 DOI: 10.5656/KSAE.2018.11.0.054

서보윤, Park chang gu, J. Jung, J. Cho, Gwan‐Seok Lee, 김광호

引用次数: 1

Ecological Replacement of Native Rapeseed Weevil (Ceutorhynchus albosuturalis ) by Invasive Alien Species, Cabbage Seedpod Weevil (C. obstrictus ) on Rapeseed Flowers in Korea 外来入侵种白菜籽象甲(C. obstrictus)在韩国油菜花上的生态替代

Korean Journal of Applied Entomology Pub Date : 2018-01-01 DOI: 10.5656/KSAE.2018.10.0.045

김경용, Lee, Won-hoon, 홍기정

引用次数: 2

First Report of Genus Hylis Gozis, 1886 (Coleoptera: Eucnemidae) with a Species, Hylis harmandi (Fleutiaux, 1923) from Korea 韩国金蝇属首次报告，1886(鞘翅目:金蝇科)及一种，金蝇属(Fleutiaux, 1923)

Korean Journal of Applied Entomology Pub Date : 2018-01-01 DOI: 10.5656/KSAE.2018.08.0.033

Jinbae Seung, S. H. Lee

引用次数: 0

Development of Monoclonal Antibodies Specific to Galectin of Pine Wood Nematode, Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle and Their Utilization for Detection of Pine Wood Nematodes 松材线虫(Bursaphelenchus xylophilus (Steiner and Buhrer) nickel)凝集素单克隆抗体的制备及其在松材线虫检测中的应用

Korean Journal of Applied Entomology Pub Date : 2018-01-01 DOI: 10.5656/KSAE.2017.11.0.044

A. Kim, Young Ha Kim, Bo-Hey Choi, T. Nguyen, K. A. Yoon, S. Lee, H. Han, Y. Koh

{"title":"Development of Monoclonal Antibodies Specific to Galectin of Pine Wood Nematode, Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle and Their Utilization for Detection of Pine Wood Nematodes","authors":"A. Kim, Young Ha Kim, Bo-Hey Choi, T. Nguyen, K. A. Yoon, S. Lee, H. Han, Y. Koh","doi":"10.5656/KSAE.2017.11.0.044","DOIUrl":"https://doi.org/10.5656/KSAE.2017.11.0.044","url":null,"abstract":"Currently, there is no available tool that rapidly diagnoses pine wood nematode (PWN)-infected pine trees in the field. In this study, we synthesized and purified PWN Galectin, which might be an antigen specific to PWN, using the Baculovirus expression system. We used PWN Galectin as an antigen for generating 1,464 fusion hybridoma cell lines secreting monoclonal antibodies (Mabs). Among them, we selected 62 fusion hybridoma cell lines showing high reactivity to PWN Galectin. We further selected 12 fusion hybridoma cell lines showing high reactivity to the standard PWN-infected pine tree phosphate buffered saline (PBS) extract. Additionally, two fusion hybridoma cell lines showing no or extremely low reactivity were used as controls. The selected fusion hybridoma cell lines were subjected to limiting dilutions for selecting and establishing Mab-secreting cell lines showing higher reactivity to the standard PWN-infected pine tree extract than to the standard normal pine tree PBS extract. Moreover, the selected fusion hybridoma cell lines were further selected based on their higher reactivity to PWN protein extracts than to three non-pathogenic nematode protein extracts. The Mab-secreting cell lines established in this study could be used to develop rapid diagnostic tools that can be used in the field or in laboratories for detecting PWN-infected pine trees or PWN.","PeriodicalId":17836,"journal":{"name":"Korean Journal of Applied Entomology","volume":"13 1","pages":"7-13"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75606844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genus Conwentzia (Neuroptera: Coniopterygidae: Coniopteryginae) New to Korea 刺蕨属(神经翅目:刺蕨科:刺蕨科)韩国新发现

Korean Journal of Applied Entomology Pub Date : 2018-01-01 DOI: 10.5656/KSAE.2018.01.1.027

Seulki Kim, J. Koo, Soowon Cho

引用次数: 0

Spread of Cyst Nematodes in Highland Chinese Cabbage Field in Gangwon-do 江原道高原白菜田包囊线虫的传播

Korean Journal of Applied Entomology Pub Date : 2018-01-01 DOI: 10.5656/KSAE.2018.11.0.037

Soon-Bae Kwon, 박동권, 원헌섭, 문윤기, Jae-Hong Lee, 김용복, 최병곤, 서현택, 고형래, 이재국, Dong Woon Lee

引用次数: 4

Monitoring Methods for Metcalfa pruinosa (Say) (Hemiptera: Flatidae) Eggs on Acacia Branches 金合欢枝上金翅金合欢卵监测方法(半翅目:黄蝶科)

Korean Journal of Applied Entomology Pub Date : 2018-01-01 DOI: 10.5656/KSAE.2018.10.0.038

최용석, 황인수, 이경주, 나미숙, 박덕기, Hwa-Young Seo

引用次数: 2

Biological Control of the Sciarid Fly, Lycoriella mali (Diptera: Sciaridae) Using Steinernema carpocapsae in a Button Mushroom Cultivation House 在棉菇栽培室内利用棉菇芽孢杆菌生物防治棉菇Lycoriella mali(双翅目:棉菇科)

Korean Journal of Applied Entomology Pub Date : 2018-01-01 DOI: 10.5656/KSAE.2018.11.0.046

최용석, Hwa-Young Seo, In-Su Whang, 이대홍

引用次数: 0