{"title":"A Prussian Blue Modified Electrode Based Amperometric Sensor for Lactate Determination","authors":"P. Kuntawong, B. Lertanantawong, C. Promptmas","doi":"10.1109/BMEiCON56653.2022.10012111","DOIUrl":"https://doi.org/10.1109/BMEiCON56653.2022.10012111","url":null,"abstract":"Abstract-Lactate is a significant indicator in sports and clinical medicine, which is useful to evaluate and diagnose the health status related to oxygen deficit situations and lactic acidosis. The development of lactate biosensors have become a major growth area for noninvasive and real-time monitoring towards maintaining performance and human health status. In the present study, a Prussian Blue modified electrode-based amperometric enzymatic biosensor is one of the promising devices for continuous lactate monitoring. The working electrode of the screen-printed carbon electrode was prepared under optimal conditions with gold nanoparticle (AuNP), Prussian Blue (PB), and lactate oxidase (LOx) enzyme followed by nafion. The determination of lactate was performed using chronoamperometry with the optimal operating potential for hydrogen peroxide $left(mathrm{H}_{2} mathrm{O}_{2}right)$ (0 V versus $mathrm{Ag} / mathrm{AgCl}$ reference electrode). The lactate calibration curve showed a linear response in the wide concentration range between $5 times 10^{-6}$ and $1 times 10^{-3} mathrm{M}$. The analytical accuracy and precision (CV%) of this lactate biosensor were 98.89 % and 1.68-3.96 %, respectively. Furthermore, irrelevant analytes and other substances which might appear in sweat were applied as a negative control to present the non-specific interaction between enzyme and substrate reaction. This study shows the success of the amperometric sensor system for lactate determination and can be used to develop for further study as a rapid response and portable point of care device.","PeriodicalId":177401,"journal":{"name":"2022 14th Biomedical Engineering International Conference (BMEiCON)","volume":"209 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114220423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wenze Wu, Xuanxuan Xie, Lin Gao, Jingxuan Liu, Bo Zhang
{"title":"Effect of Mechanical and Electrical Stimulation on the Differentiation and Orientation of C2C12 Cells","authors":"Wenze Wu, Xuanxuan Xie, Lin Gao, Jingxuan Liu, Bo Zhang","doi":"10.1109/BMEiCON56653.2022.10011584","DOIUrl":"https://doi.org/10.1109/BMEiCON56653.2022.10011584","url":null,"abstract":"The low differentiation and orientation rate of C2C12 cells is a key factor that restricts the performance improvement of the bio-hybrid robots driven by C2C12 cells. It has been demonstrated that the application of external stimuli during in the process of cell culture can contribute to C2C12 cells growth and differentiation. In this study, we applied different parameters of mechanical stimulation, electrical stimulation and coupled stimulation to C2C12 cells, and observed the differentiation and orientation of cells to investigate the optimal stimulation parameters and the effect of force-electric coupling on the differentiation and orientation of C2C12 cells. The results show that the optimal mechanical stimulation parameter is 10% static strain and electrical stimulation parameter is 1V/cm. The results of this study are a referable protocol for improving differentiation and orientation rate of C2C12 cells.","PeriodicalId":177401,"journal":{"name":"2022 14th Biomedical Engineering International Conference (BMEiCON)","volume":"404 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126679004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thanaporn Chuenpirom, Kasama Srirussamee, C. Pintavirooj
{"title":"Remote Temperature Monitoring for Infant Incubator using Thermal Camera","authors":"Thanaporn Chuenpirom, Kasama Srirussamee, C. Pintavirooj","doi":"10.1109/BMEiCON56653.2022.10012072","DOIUrl":"https://doi.org/10.1109/BMEiCON56653.2022.10012072","url":null,"abstract":"Health concerns over the premature babies are their immature organs and low birth weight. As a result, these babies may have difficulty in breathing, maintaining their healthy body temperature or being light sensitive. Infant incubator has been designed to overcome these issues. It is a medical device that could provide healthy environment, such as light, temperature and humidity. Moreover, it is also able to control and detect any changes which affect the baby’s health. Health monitoring systems are critical in modern incubators, which could involve a set of sensors to read and send physiological information, such as temperature. It has also been proposed that establishing remote monitoring system via global system for mobile communications (GSM) would be beneficial for the incubators as it could send an alarm to the doctors and parents in the event that unhealthy vital parameters have been detected. Although a variety of vital parameters are included in the monitoring features of some modern incubators, the capability to detect body temperature of the babies are limited. Hence, this project proposes the development of an effective low-cost smart infant incubator that will benefit the body temperature monitoring of newborn babies using remote IoT-based monitoring system. The monitoring system is controlled through the use of a Raspberry Pi attached to a visual camera and a thermal IR camera with temperature sensor MLX90640, which was able to measure the body temperature remotely. The software of this smart infant incubator was used to improve monitoring system, which involves image processing techniques. The results of measured ambient temperature were found to be accurate and reliable. The prototypical incubator has been designed and manufactured with affordable cost, and the control system of the incubator was able to work comparably to other incubators with potential capability to measure body temperature of the infant.","PeriodicalId":177401,"journal":{"name":"2022 14th Biomedical Engineering International Conference (BMEiCON)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131926398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xuanxuan Xie, Wenze Wu, Lin Gao, Tian Chen, Bo Zhang
{"title":"C2C12 Differentiation and Orientation Approach Towards Actuation Improvement for Biohybrid Robots","authors":"Xuanxuan Xie, Wenze Wu, Lin Gao, Tian Chen, Bo Zhang","doi":"10.1109/BMEiCON56653.2022.10012089","DOIUrl":"https://doi.org/10.1109/BMEiCON56653.2022.10012089","url":null,"abstract":"The weak maturity and poor alignment of myotube significantly limit the contractility of C2C12 mouse cell line, which is an important source of engineered skeletal muscle tissue powering biohybrid robots. Currently, substrate material, biochemical factor, topological clue and so on are proved to improve the maturing status and oriented growth of myoblasts, and co-stimulation methods stand a good chance of synergetic enhancement. Therefore, this paper invests C2C12 myogenic differentiation under triple-stimulation: soft substrate, coating and micro-groove structure. The impacts of these three factors are evaluated by the viability, orientation rate and fusion rate of C2C12 myotube, while the viability, obtained by CCK-8 test, is the basic assessment of cell proliferation; the orientation rate and the fusion rate indicates the potential for controllable contractility and the maturity of myotube, and both are analyzed and calculated from fluorescence image processing. After 7 days differentiational culture of C2C12 cell, with a GelMA substrate coated with 10 μg/cm2 fibronectin, the highest viability reaches 78.1%, and the wider (150 μm) and the shallower (25 μm) micro-groove scaffold provide more advantageous support for C2C12 differentiation and orientation. The results of this study are a referable protocol for unidirectional differentiation of C2C12 cell line based biohybrid actuator.","PeriodicalId":177401,"journal":{"name":"2022 14th Biomedical Engineering International Conference (BMEiCON)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129921597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chalinee Phiphattanaphiphop, R. Phatthanakun, K. Leksakul
{"title":"A Microfluidic Sensor for Single Cell Detection and Counting Bull Sperms Cell","authors":"Chalinee Phiphattanaphiphop, R. Phatthanakun, K. Leksakul","doi":"10.1109/BMEiCON56653.2022.10012071","DOIUrl":"https://doi.org/10.1109/BMEiCON56653.2022.10012071","url":null,"abstract":"A microfluidic sensor chip for bull sperm detection with ability of detection and counting is presented. The chip was fabricated by UV and soft lithography process in order to create electrodes and microchannel for cell alignment and delivery. Moreover, the chip was integrated with a pair of gold microelectrodes acted as a cell counter based on LCR-impedance circuit. Main objective of this specially designed circuit is reducing the large interface impedance of the microelectrodes, make them easy to use, for higher accuracy and efficiency in counting process. However other cells than those spermatozoa in semen also cause change of impedance when passing the pair of electrodes and interfere with the spermatozoa count. This work demonstrated that the change in electrical impedance is related to the size of cells passing through the electrodes, allowing distinguishable between spermatozoa and other cells, i.e. beads suspended in washing media. Work has been designed to measure average impedance of three samples; media, beads and spermatozoa investigated at signal frequency of 2 MHz, 2.5V. The averaged output impedances were 143.14 kΩ, 151.26 k$Omega$, and 152.67 k$Omega$, respectively. Then, these data were normalized as aspect to compare the differences between signal of beads and bull sperms. All analyzed values show consistencies of data with p-value lower than 0.05. This work provided important information as the device can be developed utilizing the impedance as a distinguishable cell-counter when spermatozoa and beads flow pass the area of detecting electrodes.","PeriodicalId":177401,"journal":{"name":"2022 14th Biomedical Engineering International Conference (BMEiCON)","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130281234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Narkbuakaew, Kongyot Wangkaoom, Duangkamol Banarsarn, Chalinee Thanasupsombat, S. Iamsiri, S. Thongvigitmanee
{"title":"A Combination of a 3D Surface Model and CBCT Images for Dental Applications Using VTK Library","authors":"W. Narkbuakaew, Kongyot Wangkaoom, Duangkamol Banarsarn, Chalinee Thanasupsombat, S. Iamsiri, S. Thongvigitmanee","doi":"10.1109/BMEiCON56653.2022.10012067","DOIUrl":"https://doi.org/10.1109/BMEiCON56653.2022.10012067","url":null,"abstract":"In dental applications, information of the soft-and-hard tissues’ surfaces from an intraoral scan or a dental cast helps a user to enhance visualization of anatomical structures in cone-beam computed tomography (CBCT) images. This paper presents a combination process based on the Visualization Toolkit (VTK) library. The proposed process consisted of three main steps: landmark identification, 3D surface registration, and visualization of alignment’s quality. In experiments, the tissue’s surfaces were acquired from intraoral scans and dental casts, and these surfaces were combined with several CBCT data. The results showed that all three parts of the proposed process performed smoothly. A combination of CBCT data and tissue’s surfaces obviously presented good quality of alignment and improved visualization of anatomical structures although qualities of CBCT data were dropped by image noises and some artifacts.","PeriodicalId":177401,"journal":{"name":"2022 14th Biomedical Engineering International Conference (BMEiCON)","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125074916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. V. Guglielmi, Giulia Cisotto, T. Erseghe, L. Badia
{"title":"Frequency-Dependent Functional Connectivity of Brain Networks at Resting-State","authors":"A. V. Guglielmi, Giulia Cisotto, T. Erseghe, L. Badia","doi":"10.1109/BMEiCON56653.2022.10012100","DOIUrl":"https://doi.org/10.1109/BMEiCON56653.2022.10012100","url":null,"abstract":"Functional imaging methods such as resting-state fMRI allow to describe interactions among different areas of the brain, thus deriving a functional connectivity matrix of the entire brain network. Tracking functional relationships among different regions of interest can be applied, besides a pure modelling perspective, also to discovering procedures to detect brain diseases and anomalies, or pursuing rehabilitation of subjects with structural damages. However, network characterization is often regarded as frequency-independent, so that the frequency at which interactions take place among different regions is ignored. In this paper, we show how simple filtering procedures over different bands, applied to the resting-state fMRI signals, result in highly different connectivity matrices. Thus, it is highlighted that the functional network can be significantly dependent on the considered frequency range for the fMRI signal. This both justifies the need for a careful filtering of the signals, that avoids filtering out relevant frequencies, and also hints the possibility of classifying functional interactions according to the frequency where the connectivity among two areas is the strongest.","PeriodicalId":177401,"journal":{"name":"2022 14th Biomedical Engineering International Conference (BMEiCON)","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125182619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Automatic Cardiopulmonary Resuscitation System","authors":"Rawin Keakultanes, M. P. Paing, C. Pintavirooj","doi":"10.1109/BMEiCON56653.2022.10012076","DOIUrl":"https://doi.org/10.1109/BMEiCON56653.2022.10012076","url":null,"abstract":"Cardiac arrest occurs when the heart unexpectedly malfunctions and stops breathing and pumping blood. Cardiopulmonary resuscitation, an emergency resuscitation procedure, must be performed as soon as possible when the patients suffer cardiac arrest to prevent brain damage or stop breathing. If not, it may lead a person dead even in a short time (from 8-10 minutes) of cardiac arrest. According to the American Heart Association, proper CPR must be performed at a depth of 5 cm in the sternum and a rate of chest compressions of 100 to 120 breaths per minute. Therefore, medical personnel or the person who performs CPR must be well-trained to conduct proper CPR. For this reason, CPR machines are demanded to assist and support medical personnel in saving human lives. This paper will focus on developing an automated CPR machine and automatic resuscitation ventilator to ensure that patients receive proper CPR. The device will be wirelessly controlled by a mobile phone via Bluetooth and was able to press at a depth of 4-5 cm, which is a correct depth, and at a rate of 70 beats per minute. An Arduino Uno microcontroller and a linear motor are used to control the push of the device. The proposed automated CPR machine will be a cost-effective, fully functional CPR device that is easy to use and can help people or healthcare professionals perform proper CPR.","PeriodicalId":177401,"journal":{"name":"2022 14th Biomedical Engineering International Conference (BMEiCON)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130447208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Chiangnoon, P. Karawak, J. Eamsiri, N. Thamrongsiripak, P. Uttayarat, W. Angkhasirisap, S. Ampawong
{"title":"Preparation of biocompatible PVA hydrogels as wound dressings by gamma irradiation","authors":"R. Chiangnoon, P. Karawak, J. Eamsiri, N. Thamrongsiripak, P. Uttayarat, W. Angkhasirisap, S. Ampawong","doi":"10.1109/BMEiCON56653.2022.10012079","DOIUrl":"https://doi.org/10.1109/BMEiCON56653.2022.10012079","url":null,"abstract":"Hydrogels have emerged as a new class of materials that are capable of providing appropriate moist environment for wound healing. In this report, gamma irradiation was utilized to prepare flexible, self-adhesive, and biocompatible hydrogel sheets from polyvinyl alcohol (PVA) as wound dressings. The PVA concentrations ranged from 6-10 % (w/v) and gamma doses from 10-80 kGy were first varied to optimize the formation of crosslinked, three-dimensional structure of hydrogels. Based on swelling behavior, the 10% (w/v) PVA solution crosslinked at the dose of 40 kGy was selected to fabricate prototypic hydrogel sheets that could conform over skin contour. The extraction-based MTT assay showed that the hydrogel sheets were cytocompatible with L929 cells. When applied on full-thickness defects in rat model, the hydrogel sheets could facilitate re-epithelization to complete wound closure. These results demonstrate that our PVA hydrogel sheets developed by gamma irradiation can serve as a suitable candidate for wound dressings.","PeriodicalId":177401,"journal":{"name":"2022 14th Biomedical Engineering International Conference (BMEiCON)","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117025196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kohtaro Hongo, Zugui Peng, K. Shimba, Y. Miyamoto, T. Yagi
{"title":"Development of a Battery Using a Hydrogel Network","authors":"Kohtaro Hongo, Zugui Peng, K. Shimba, Y. Miyamoto, T. Yagi","doi":"10.1109/BMEiCON56653.2022.10012086","DOIUrl":"https://doi.org/10.1109/BMEiCON56653.2022.10012086","url":null,"abstract":"Biocompatible power sources are essential for implanting electronic devices in the body. One promising technology is batteries based on droplet networks. In this method, two droplets of different solute concentrations coated with lipid monolayers are combined in oil. An anion-selective membrane protein is then inserted into the lipid bilayer formed between the two droplets to generate an electric current. However, the droplet interface bilayer is vulnerable to external stimuli and the droplets tend to fuse. To solve this problem, we herein use a hydrogel network composed of gels separated by a lipid bilayer. Gels are more rigid than droplets, thus enabling the construction of robust networks. We first evaluate the robustness of the hydrogel network. Next, we verify the occurrence of ion transport in the network. Finally, we create a battery using the hydrogel network.","PeriodicalId":177401,"journal":{"name":"2022 14th Biomedical Engineering International Conference (BMEiCON)","volume":"200 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116155438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}