Journal of Tropical Life Science最新文献_第9页

Vitamin D Profile and Disease Activity of JIA During Pandemic Covid-19 Covid-19大流行期间JIA的维生素D谱与疾病活性

Journal of Tropical Life Science Pub Date : 2022-05-17 DOI: 10.11594/jtls.12.02.05

D. Wulandari, W. Barlianto, T. Sari

{"title":"Vitamin D Profile and Disease Activity of JIA During Pandemic Covid-19","authors":"D. Wulandari, W. Barlianto, T. Sari","doi":"10.11594/jtls.12.02.05","DOIUrl":"https://doi.org/10.11594/jtls.12.02.05","url":null,"abstract":"Vitamin D (25-Hydroxyvitamin D3 [25(OH)D3]) has an important role in the immune system. This study aimed to assess the relationship between 25-Hydroxyvitamin D3 and disease activity in Indonesian children with Juvenile Idiopathic Arthritis (JIA) during pandemic Covid-19. A 12-week randomized control trial was undertaken at Saiful Anwar Hospital between June and September 2021. JIA patients are divided into two groups. The control group (CG), without supplementation, and the treatment group (TG) got a high dose of oral cholecalciferol 2000 IU/day. Serum levels of 25(OH)D3 were measured using an enzyme-linked immunosorbent assay (ELISA). Comparison between serum 25(OH)D3 levels and JIA subtypes, peripheral blood Creactive protein (CRP), erythrocyte sedimentation rate (ESR), and Juvenile Arthritis Disease Activity Score (JADAS-27 Score) were analyzed using SPSS. There was a significant increase in serum 25(OH)D3 levels in treatment group (mean: 27.38 ± 6.39 ng/ml vs 42.26 ± 10.95 ng/ml; P=0.000). The JADAS-27 score significantly decrease in treatment group (14.60 ± 4.04 vs 6.24 ± 1.96; p= 0.000). Serum 25(OH)D3 showed a significant correlation with the JADAS-27 score. The levels of 25(OH)D3 are significantly decreased in children with JIA. Decreased 25(OH)D3 levels may be associated with the etiopathogenesis of JIA. Vitamin D levels have correlations with disease activity. Adjunctive treatment of cholecalciferol improves the disease activity in JIA patients. Keywords: Disease activity, JIA, Pandemic Covid-19, Vitamin D","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48117913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect Combination of Catharanthus roseus and Phyllanthus urinaria Extract on Mice BALB/c Breast Cancer Models Based on T-regulator Cells Expression 基于T调节细胞表达的长春花和叶下珠提取物联合对小鼠BALB/c乳腺癌症模型的影响

Journal of Tropical Life Science Pub Date : 2022-05-17 DOI: 10.11594/jtls.12.02.11

Aya Shofiyah, M. Djati, Muhaimin Rifa’i

{"title":"Effect Combination of Catharanthus roseus and Phyllanthus urinaria Extract on Mice BALB/c Breast Cancer Models Based on T-regulator Cells Expression","authors":"Aya Shofiyah, M. Djati, Muhaimin Rifa’i","doi":"10.11594/jtls.12.02.11","DOIUrl":"https://doi.org/10.11594/jtls.12.02.11","url":null,"abstract":"CD4+CD25+FOXP3+ is a regulatory T cell that plays a role in the body's tolerant system to control activated effector cells. T regulatory plays an important role as a trigger for tumor progressiveness, where an increase in the amount of T regulatory FOXP3 in the tumor state correlates with a poor prognosis. Breast cancer is a type of cancer that tends to increase every year. The purpose of this study was to study the influence of a combination of Phyllanthus urinaria and Catharanthus roseus plant extracts on CD4+CD25+FOXP3+ modulation. Mice was injected subcutaneously with the carcinogen compound DMBA for six weeks at 0.015 mg/kg weight. The study design was conducted using a complete random design (RAL) which was divided into six groups namely healthy (normal) control, cancer control (DMBA injection), Cisplatin and a combination of extracts with three different doses (dose 1 (Phyllanthus urinaria 500 weight and Catharanthus roseus 75mg/kg weight) and dose 3 (Phyllanthus urinary 2000mg/kg weight and Catharanthus roseus 375mg/kg weight). The relative number of T regulatory cells is measured using flow cytometry analysis. The measurements were made using the Cell Quest ProTM program. Statistics analyze using one way ANOVA (p<0.05). Tukey HSD analysis with SPSS 26 software used to analyze real differences between treatments. A relative increase in CD4+CD25+FOXP3+ occurs in cancer control. While the decrease occurred in every treatment except in the dose group 1. The optimal dose to reduce CD4+CD25+FOXP3+ amounts significantly occurred in the dose group 3. This indicates the influence of a combination of Phyllanthus urinaria and Catharanthus roseus on CD4+CD25+FOXP3+ differentiation. Keywords: breast cancer, Catharanthus roseus, CD4+CD25+FOXP3+, Phyllanthus urinaria,","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46451370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of a Bacterium from Selat Tebrau and Screening of Microbial Genomes for Dehalogenases Selat Tebrau一株细菌的鉴定及脱卤酶微生物基因组的筛选

Journal of Tropical Life Science Pub Date : 2022-05-17 DOI: 10.11594/jtls.12.02.06

H. Oyewusi, Raja Mohamed, F. Huyop

{"title":"Characterisation of a Bacterium from Selat Tebrau and Screening of Microbial Genomes for Dehalogenases","authors":"H. Oyewusi, Raja Mohamed, F. Huyop","doi":"10.11594/jtls.12.02.06","DOIUrl":"https://doi.org/10.11594/jtls.12.02.06","url":null,"abstract":"Current study was to investigate the presence of dehalogenase in the isolated bacterium from Selat Tebrau that can grow on 2,2-dichloropropionic acid (2,2-DCP is an active compound in herbicide Dalapon®). Strain RN1, a Gram-negative and rod in shape was tentatively identified as Enterobacter cancerogenus based on basic biochemical and the 16S rRNA gene analyses. The calculated cells doubling time were 5.29 hours based on growth of the bacterium in liquid minimal media with 10, 20 and 30 mM of 2,2-DCP, respectively. However, no growth was observed at 40 mM 2,2-DCP liquid minimal medium due to increase in 2,2-DCP toxicity. It was hypothesized that, strain RN1 produced dehalogenase(s) which merits a further study of the genomic data of the same genus and species available in the database. A putative dehalogenase, designated as DehRN was located in the published data of Enterobacter cancerogenus. Pairwise of DehRN amino acids with known dehalogenase resulted in sequence identity of <20%, suggesting a new class of dehalogenase enzyme in the Enterobacter cancerogenus.Keywords: 2,2-DCP, dehalogenase, dichloropropionate, Enterobacter, haloalkanoic acid","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43613940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemicals, Antioxidant and Anticancer Properties of Camellia japonica L. Mistletoe Extracts 槲寄生提取物的植物化学成分、抗氧化和抗癌特性

Journal of Tropical Life Science Pub Date : 2022-05-17 DOI: 10.11594/jtls.12.02.13

M. Kin, J. Kim

{"title":"Phytochemicals, Antioxidant and Anticancer Properties of Camellia japonica L. Mistletoe Extracts","authors":"M. Kin, J. Kim","doi":"10.11594/jtls.12.02.13","DOIUrl":"https://doi.org/10.11594/jtls.12.02.13","url":null,"abstract":"The present study aims to investigate the phytochemical profiles, in vitro antioxidant and antiproliferative properties of methanol and 70% ethanol extracts of Camellia mistletoe. Both extracts were analyzed for contents of total flavonoid, total carotenoid and L-ascorbic acid, and antioxidant properties such as scavenging capacities (1,1-diphenyl-2-picrylhydrazyl, O2- and NO), ferrous ion chelating and reducing power. MTT assay was used to assess the antiproliferative properties against human cancer cell lines. The results showed that the methanol extracts of Camellia mistletoe contained higher total flavonoids (16237 mg rutin equivalents/100 g) and carotenoids (49175 mg/100g) with higher DPPH (IC50 = 0.6 mg/mL), superoxide (IC50 = 0.6 mg/mL), nitric oxide (IC50 = 0.5 mg/mL) radical scavenging and reducing power (IC50 = 1.1 mg/mL) activities than those of ethanol extracts (p < 0.05). In addition, methanol extracts showed much higher antiproliferative activity against A375 (IC50 = 118.1 μg/mL) and HCT116 (IC50 = 148.4 μg/mL) cells than ethanol extracts whereas higher inhibitory effects of MCF7 (IC50 = 139.9 μg/mL), Hela (IC50 = 127.1 μg/mL) and HepG2 (IC50 = 84.2 μg/mL) cell proliferation in the ethanol extracts of Camellia mistletoe. The data from this study suggest that Camellia mistletoe is a potential source of phytochemical compounds with antioxidant and antiproliferative properties.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49125034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular Docking and Interaction Analysis of Propolis Compounds Against SARS-CoV-2 Receptor 蜂胶化合物对抗严重急性呼吸系统综合征冠状病毒2型受体的分子对接和相互作用分析

Journal of Tropical Life Science Pub Date : 2022-05-17 DOI: 10.11594/jtls.12.02.08

M. Syaban, I. Faratisha, K. Yunita, N. Erwan, Dedy Kurniawan, G. Putra

{"title":"Molecular Docking and Interaction Analysis of Propolis Compounds Against SARS-CoV-2 Receptor","authors":"M. Syaban, I. Faratisha, K. Yunita, N. Erwan, Dedy Kurniawan, G. Putra","doi":"10.11594/jtls.12.02.08","DOIUrl":"https://doi.org/10.11594/jtls.12.02.08","url":null,"abstract":"Background: For many people, especially in developing countries, herbal medicine is the most traditional drug choice to treat all diseases including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 infection). Propolis is one of the popular herbal medicine which has various health benefits, particularly antiviral activity. In this molecular docking study, this investigation examined twenty-five kinds of propolis to bind SARS-CoV-2 protein with the main targets of ACE-2 and M-Pro receptors. Method: Propolis ligands were downloaded from PubChem, meanwhile ACE-2 and M-Pro receptors were downloaded from Protein Data Bank. Both ligands and targets were optimized by Pymol. The pharmacokinetic analysis was conducted using SwissADME. Molecular docking was done using PyRx 0.9 and its binding interaction was visualized by Discovery Studio. To predict the potential inhibition, this study compared the ligand-protein complex of propolis to ligands from the previous study. Result: Through the Lipinski rule, only five of twenty-five types of propolis were not qualified for the criterion. The ability to bind protein targets were various between ligands, the highest affinity to ACE-2 receptors were abietic acid, galangin, chrysin, kaempferol and acacetin, respectively. The binding affinity between ligand and M-Pro were seen weaker than ACE-2 receptor, while the strongest were kaempferol, abietic acid, acacetin, galangin and chrysin, respectively. Conclusion: Â Kaempferol is the most potent form of propolis to bind to ACE-2 and M-Pro receptors by assessing the binding affinity and the amount of amino acid residue formation when compared to control ligands. Keywords: ACE-2 receptor, COVID-19, Main protease, Molecular docking, Propolis, SARS-CoV-2","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41824474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of Acute Toxicity of Commercial Organophosphate Insecticide Based on Chlorpyrifos on Fejervarya limnocharis Tadpoles (Anura: Dicroglossidae) 以毒死蜱为基础的市售有机磷杀虫剂对林夜蛾蝌蚪急性毒性的影响

Journal of Tropical Life Science Pub Date : 2022-05-17 DOI: 10.11594/jtls.12.02.09

Shima Ramadani, A. Marhendra, D. Wiadnya, N. Kurniawan

{"title":"Effect of Acute Toxicity of Commercial Organophosphate Insecticide Based on Chlorpyrifos on Fejervarya limnocharis Tadpoles (Anura: Dicroglossidae)","authors":"Shima Ramadani, A. Marhendra, D. Wiadnya, N. Kurniawan","doi":"10.11594/jtls.12.02.09","DOIUrl":"https://doi.org/10.11594/jtls.12.02.09","url":null,"abstract":"Organophosphate pesticides, especially chlorpyrifos, are one of the most widely used insecticides in agriculture, but their toxicity and potential sensitivity effects on Anura, especially Fejervarya limnocharis are still unknown. The purpose of this investigation is to study F. limnocharis (Anura: Dicroglossidae) tadpole sensitivity to lethal (survivability) and sublethal effects (morphological alterations and swimming activity) of chlorpyrifos in Dursban 200EC commercial formula under acute exposure. An acute toxicity test was carried out on ten tadpoles (Gosner 25) in each repetition. The sample was obtained from artificial reproduction by injecting the Trial Batch 2000 IU hCG by Kings Lab. The acute toxicity testing consisted of three replicates with a chlorpyrifos concentration of 0; 0.4; 0.8; 1; 2; 4; and 8 µg/L. Physico-chemical parameters, mortality, morphological, and swimming alterations of each concentration were observed at 24th, 48th, 72nd, and 96th hours. The LC50 of chlorpyrifos for F. limnocharis tadpoles was 2.86 µg/L. The percentage of survivability F. limnocharis tadpoles decreased after exposure to chlorpyrifos above 1µg/L, while morphological alterations were observed in 2 µg/L and 4 µg/L after 48th hours exposure, and the swimming alterations have occurred at 24th hours in 1; 2; 4 and 8 µg/L. Morphological alterations were observed including asymmetrical body shape, edema, and abnormal tail shape. Based on the LC50 value, commercial chlorpyrifos has high-level toxicity on F. limnocharis tadpoles.Keywords: Acute, Chlorpyrifos, Dursban 200 EC, Fejervarya limnocharis, Tadpoles","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48892251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Vitamin D3 Supplementation on the Level of 25(OH)D3, Osteocalcin, and HbA1c in Children with Type 1 Diabetes Mellitus 补充维生素D3对1型糖尿病患儿25（OH）D3、骨钙素和HbA1c水平的影响

Journal of Tropical Life Science Pub Date : 2022-05-17 DOI: 10.11594/jtls.12.02.14

Hajeng Wulandari, H. Tjahjono, E. Widjajanto

{"title":"Effect of Vitamin D3 Supplementation on the Level of 25(OH)D3, Osteocalcin, and HbA1c in Children with Type 1 Diabetes Mellitus","authors":"Hajeng Wulandari, H. Tjahjono, E. Widjajanto","doi":"10.11594/jtls.12.02.14","DOIUrl":"https://doi.org/10.11594/jtls.12.02.14","url":null,"abstract":"Type 1 diabetes mellitus (T1DM) is a chronic disease caused by insulin deficiency resulting from autoimmune destruction of pancreatic beta cells. Increased risk of fractures and osteoporosis in T1DM is associated with reducing osteoblast proliferation, survival, and activity. Osteocalcin (OC) is a marker of late-stage osteoblast differentiation. It might be positively modulated by vitamin D. This study was aimed to investigate the effect of vitamin D3 supplementation on the level of 25(OH)D3, HbA1c, and OC in children with T1DM. A randomized clinical trial was conducted to measures the levels of 25(OH)D3, HbA1c, and OC in children with T1DM supplemented with vitamin D3. Many 26 children with T1DM were equally assigned into two groups: control (standardized insulin treatment) and treatment group (standardized insulin treatment + vitamin D3 2000 IU/day). Subject characteristics showed that the subjects were predominantly female (15/26), well-nourished (19/26), the average age is 12,31 ± 3,069 year, and the dose of insulin average is 1,17 ± 0,233 IU/kg BW/day. Our data demonstrated that vitamin D3 treatment significantly increased the level of 25(OH)D3 and improved glycemic control, represented by a low HbA1c level. However, there was no significant difference in OC level in both groups (independent t-test, p > 0.05). Further analysis showed no significant relationship between the level of 25(OH)D3, HbA1c, and OC. We concluded that vitamin D3 supplementation improves children's glycemic control with T1DM, but no significant alteration on OC levels.Keywords: HbA1c, Osteocalcin, T1DM, Vitamin D","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44226772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Ethnobotanical Study and Conservation Status of Plants Used by the Tigwahanon-Manobo in Mt. Malimumu, San Fernando, Bukidnon, Philippines 菲律宾圣费尔南多Malimumu山Tigwahanon-Manobo利用植物的民族植物学研究与保护现状

Journal of Tropical Life Science Pub Date : 2022-05-17 DOI: 10.11594/jtls.12.02.02

M. Dapar, Amoroso Victor

{"title":"Ethnobotanical Study and Conservation Status of Plants Used by the Tigwahanon-Manobo in Mt. Malimumu, San Fernando, Bukidnon, Philippines","authors":"M. Dapar, Amoroso Victor","doi":"10.11594/jtls.12.02.02","DOIUrl":"https://doi.org/10.11594/jtls.12.02.02","url":null,"abstract":"Central Mindanao, Philippines, is a center of the cultural wealth of knowledge and botanical resources in the southern archipelago. However, traditional plant resources remain unexplored, and most of the tribal communities remain undocumented. Manobo tribe is one of the most populated and diverse tribal communities in the country, including the secluded Tigwahanon-Manobo community in Mt. Malimumu, San Fernando, Bukidnon, Philippines. Hence, this study provides ethnobotanical information and conservation status of plants along a 1-km transect walk in Mt. Malimumu used by the Tigwahanon-Manobo. A guided field walk with the tribal chieftains and tribal healer documented a total of 23 species in 22 genera belonging to 20 families, of which four species, namely Dillenia philippinensis Rolfe, Nepenthes cornuta Marwinski, Coritico, Wistuba, Micheler, Gronem., Gieray & V.B.Amoroso, Nepenthes truncata Macfarl., and Pandanus mindanaensis Martelli, are Philippine endemics. Three families, namely Nepenthaceae, Melastomataceae, and Rubiaceae, are the most represented with two species each. Based on the combined international (IUCN 2021) and national (DENR DAO 2017-11) listing of conservation status, one species was assessed as Critically Endangered (Aquilaria malaccensis Lam.) and another one as Endangered (N. truncata). Two species were assessed as Vulnerable (Agathis philippinensis Warb. and N. cornuta) and one species as Near Threatened (D. philippinensis). Six species were identified as Least Concern while the rest of the species were not yet evaluated. This participatory research provides the first ethnobotanical study and conservation status of traditional plants used by Tigwahanon-Manobo for food and medicine, as well as economic and ecological uses needing conservation and protection of their natural resources. Keywords: Conservation, Ethnobotany, Food plants, Indigenous, Medicinal plants, Mindanao","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44649292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fish Biodiversity and Water Quality of Tropical Forest Streams Adjacent to the Western Boundary of Kinabalu Park, Sabah 沙巴州基纳巴卢公园西部边界附近热带森林溪流的鱼类生物多样性和水质

Journal of Tropical Life Science Pub Date : 2022-05-17 DOI: 10.11594/jtls.12.02.12

C. Soo, H. Mahsol, Leonardo Jainih, Arman Hadi Fikri, Cheng Chen, Nashid Kamal

{"title":"Fish Biodiversity and Water Quality of Tropical Forest Streams Adjacent to the Western Boundary of Kinabalu Park, Sabah","authors":"C. Soo, H. Mahsol, Leonardo Jainih, Arman Hadi Fikri, Cheng Chen, Nashid Kamal","doi":"10.11594/jtls.12.02.12","DOIUrl":"https://doi.org/10.11594/jtls.12.02.12","url":null,"abstract":"Modification and degradation of habitats outside the boundaries of a national parkmay result in the park's integrity being compromised. Thus, the present study aims todocument variation in freshwater fish and water quality of tropical forest streams adjacent to the western boundary of Kinabalu Park, Sabah. Concurrently, fish samplesand environmental parameters were collected at six stations covering a total streamline length of 10 km. The three most abundant species were Tor tambra (34.4%), Barbodes sealei (19.5%), and Lobocheilos ovalis (12.4%). Tor tambra exhibited a negative allometric growth pattern (coefficient < 3) and was in poor to fair condition (0.8< Fulton’s condition factor < 1.2). The highest fish abundance was found in downstream stations with species addition. Metrics of diversity, such as Shannon's diversityindex and the Margalef index of species richness, decreased as elevation increasedfrom 100 to 600 metres above sea level. The water quality of the forest streams isgood, classified as Class I and/or Class II by Malaysia's National Water Quality Standards, except for ammoniacal nitrogen at station 6, which was classified as Class III.Higher turbidity, orthophosphate, and ammoniacal-nitrogen values were found nearthe Podos village. In conclusion, the altitudinal gradient of fish biodiversity was observed in the forest streams near Kinabalu Park. Regular water quality monitoring ofthe downstream river is necessary, as indicated by the potential domestic impacts onthe river.Keywords: Biodiversity, Elevation, Freshwater fish, Forest stream, Kinabalu Park","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41977297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Analysis for Haloacid Dehalogenase in Bacillus megaterium WSH-002 巨型芽孢杆菌WSH-002中卤酸脱卤酶的基因组分析

Journal of Tropical Life Science Pub Date : 2022-01-31 DOI: 10.11594/jtls.12.01.07

L. Zulkarnain, F. Huyop

{"title":"Genomic Analysis for Haloacid Dehalogenase in Bacillus megaterium WSH-002","authors":"L. Zulkarnain, F. Huyop","doi":"10.11594/jtls.12.01.07","DOIUrl":"https://doi.org/10.11594/jtls.12.01.07","url":null,"abstract":"Bacterial dehalogenation is one of the processes that can reduce environmental pollutions. The attributes of B. megaterium that can grow in a polluted environment suggested that its genome contains pollutant degrading genes. To date, there were no reports related to dehalogenase in B. megaterium WSH-002 and how it was regulated. Therefore, the presence of environmentally important genes that can detoxify organohalogens in many microbial genomes, including B. megaterium WSH-002 will be investigated. The genome sequence of B. megaterium WSH-002 was retrieved from NCBI databases. It was then annotated through the RAST server to identify all the putative dehalogenase gene sequences. The selected gene sequence was converted into amino and went through BLASTp via UniProt database. The highest percentage identity of the amino acid sequence to any dehalogenases was subjected to further identification of specific dehalogenase domain using InterPro Scan server. The results from genome annotations have shown its potential for bioremediation due to the presence of putative dehalogenase protein. Only one type of haloacid dehalogenase was identified. It was classified as haloacid dehalogenase type II because its amino acid sequence is highly identical with HAD_type_II and HAD_L2-DEX. The study concluded that the genome of B. megaterium WSH-002 contains a haloacid dehalogenase gene that is useful for the biodegradation of halogenated compounds. In the future, further investigation on the expression of the dehalogenase gene as recombinant protein and to study its protein structure and functions will be considered.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48245737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1