Journal of Tropical Life Science最新文献_第4页

The selection and the Growth Condition Optimization of Ethanol‐Producing Microbes Isolated from Ragi Tapai 龙葵产乙醇菌的筛选及生长条件优化

Journal of Tropical Life Science Pub Date : 2023-05-25 DOI: 10.11594/jtls.13.02.12

Ida Bagus, Wayan Gunam, Gede Arya Sujana, I. Made, Mahaputra Wijaya, Y. Setiyo, Wayan Arnata, Wayan Wisma, Pradnyana Putra

{"title":"The selection and the Growth Condition Optimization of Ethanol‐Producing Microbes Isolated from Ragi Tapai","authors":"Ida Bagus, Wayan Gunam, Gede Arya Sujana, I. Made, Mahaputra Wijaya, Y. Setiyo, Wayan Arnata, Wayan Wisma, Pradnyana Putra","doi":"10.11594/jtls.13.02.12","DOIUrl":"https://doi.org/10.11594/jtls.13.02.12","url":null,"abstract":"Ethanol is a biofuel produced from renewable resources, which potentially plays an important role in solving future fuel problems. This study aimed to select the highest ethanol-producing isolate from candidates obtained from previously isolated candidates from ragi and cassava tapai. The selection process was conducted in 2 stages, namely: 1) Selection of the highest ethanol-producing isolate from seven isolate candidates using PYG media containing peptone, yeast extract, and glucose at 0.75%, 0.75%, and 15%, respectively and was followed by: 2) Optimization of the growth conditions of the highest ethanol-producing isolate, which was conducted at various temperatures of 27, 30, 33, 35, 37, and 40°C with the combination of various pH of 3.5, 4.5, 5.5, and 6.5. The experimental results showed that the R5I3 isolate was the highest ethanol-producing performance isolate, which yielded approximately 4.69±0.25% (v/v). Following the temperature and pH optimization of the fermentation processes, the optimum growth conditions were at 35°C and pH 5.5, where the ethanol produced was increased to 8.63 ± 0.04% (v/v). With these results, this new strain has the potential to be used in bioethanol production processes and other industrial applications.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42438300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Ultrasonic Amplitude on the Yield and Properties of Barramundi (Lates calcarifer) Skin Collagen 超声振幅对梭鲈皮肤胶原蛋白产量和性质的影响

Journal of Tropical Life Science Pub Date : 2023-05-25 DOI: 10.11594/jtls.13.02.03

U. Razali, Ainaa Juraimy, Y. Jusoh, D. Dailin, H. Ya’akob, Noorazwani Zainool, D. Zaidel

{"title":"Effect of Ultrasonic Amplitude on the Yield and Properties of Barramundi (Lates calcarifer) Skin Collagen","authors":"U. Razali, Ainaa Juraimy, Y. Jusoh, D. Dailin, H. Ya’akob, Noorazwani Zainool, D. Zaidel","doi":"10.11594/jtls.13.02.03","DOIUrl":"https://doi.org/10.11594/jtls.13.02.03","url":null,"abstract":"Barramundi skin, a by-product of the fish processing industry, has shown potential as an alternative collagen source. However, the commonly used acid extraction method to produce collagen rendered a low yield requires a lengthy time and is not environmentally friendly. As a result, the adoption of greener technology, such as ultrasound, to improve the conventional extraction process is emerging. This study aimed to investigate the effect of different ultrasonication amplitudes on collagen recovery from barramundi skin. The resulting collagens were evaluated for their protein, hydroxyproline and moisture content, colour, molecular weight distribution, and FTIR spectra. Ultrasound-assisted extraction (UAE) was performed at 40 (UAE40), 60 (UAE60) and 80 (UAE80) % amplitude for 20 min. For comparison, acetic acid extraction was also carried out to produce acid-soluble collagen (ASC). UAE increased the yield (p<0.05) of collagen from barramundi skin, with UAE80 exhibiting a 7-fold increment compared to ASC. Increasing the ultrasonic amplitude increased the yield considerably but decreased the hydroxyproline content, indicating a reduction in collagen quality. Furthermore, the protein content and SDS-PAGE profile of the extracted collagens revealed that UAE promoted protein degradation. FTIR spectra indicated that despite slightly varying wavenumbers, no detrimental effect on the triple helical structure was seen following UAE with the presence of amides A, B, I, II, and III. Also, the α1, α2 and ß-chains were found in all samples, although the band intensity reduced as the amplitude increased. In conclusion, given the right conditions, UAE could improve the extraction yield without influencing the collagen structure.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42838667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Genetic Analysis of Momordica cochinchinensis (Lour.) Spreng. (Gac) from Different Accessions in Malaysia 番荔枝(Momordica cochinchinensis, Lour.)的形态与遗传分析Spreng。(Gac)从不同的加入在马来西亚

Journal of Tropical Life Science Pub Date : 2023-05-25 DOI: 10.11594/jtls.13.02.10

N. Khairi, H. Othman

{"title":"Morphological and Genetic Analysis of Momordica cochinchinensis (Lour.) Spreng. (Gac) from Different Accessions in Malaysia","authors":"N. Khairi, H. Othman","doi":"10.11594/jtls.13.02.10","DOIUrl":"https://doi.org/10.11594/jtls.13.02.10","url":null,"abstract":"Momordica cochinchinensis or gac fruit is a 'superfruit' that is well-known in Vietnam. Gac is an orange fruit that is ovoid in shape and has a soft spiny texture. In Malaysia, gac fruit is a new and less known plant. This study aimed to characterize gac fruit using morphological analysis involving both vegetative and reproductive parts and to characterize the genetic diversity in gac fruit by using Inter-simple sequence repeat (ISSR) analysis. Four different gac accessions were collected from different areas (Kota Damansara (Selangor), Melaka Tengah (Melaka), Hulu Langat (Selangor) and Kuantan (Pahang)) were cultivated under tropical conditions in Kuantan, Pahang. The gac accessions showed differences in morphological characters. Generally, the gac fruits were reddish-orange in colour, the leaf was dark green on the adaxial part and light green on the abaxial part, and the female and male flower was light yellow and white in color. The fruit weight ranged from 193.72 g (GD) to 334.70 g (GH) with varied shapes and spike density. DNA extraction was following the CTAB method. All 30 primers showed high levels of polymorphism (83%) and the polymorphism information content (PIC) with the mean of 0.48. Nei's genetic distance coefficient ranged between 0.27 and 0.6 with the mean value of 0.41. Dendrogram based on UPGMA analysis grouped the four gac accessions into two main groups. Cluster I consisted of accession GD, GM and GH while cluster II consisted of only GX. Results from both morphological and molecular analysis showed genetic diversities in all four gac fruits studied.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45424949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proximate Composition and Antimicrobial Activity of Kefir Produced from Cow’s and Almond Milk 由牛奶和杏仁奶制成的开菲尔的近似组成和抗菌活性

Journal of Tropical Life Science Pub Date : 2023-05-25 DOI: 10.11594/jtls.13.02.06

Jing Hew, S. Shafie, N. Sulaiman

{"title":"Proximate Composition and Antimicrobial Activity of Kefir Produced from Cow’s and Almond Milk","authors":"Jing Hew, S. Shafie, N. Sulaiman","doi":"10.11594/jtls.13.02.06","DOIUrl":"https://doi.org/10.11594/jtls.13.02.06","url":null,"abstract":"Fermented foods are an important diet component of people around the world. Kefir, or fermented milk, is popular worldwide due to its high nutritional value, with cow's milk being the common substrate for traditional kefir fermentation. However, the scarcity of animal-based milk in some countries, plus cultural, religious, and health reasons, have seen non-dairy milk kefir from almond milk gaining popularity among consumers globally. This study aimed to evaluate and compare the proximate composition and anti-microbial activity of kefir produced from 100% cow or 100% almond or an equal (1:1) mixture of both kinds of milk. This study used the AOAC 2000 method for the proximate analysis, while the agar well diffusion method examined the anti-microbial activity of the milk samples against Escherichia coli, Staphylococcus aureus, and Salmonella typhi. Results revealed that the three kefir samples showed significantly different (p<0.05) moisture, total dietary fiber, and fat contents and were within the CODEX acceptable range for kefir. All samples exhibited varying degrees of inhibition between the different pathogens. The diameters of the inhibition zone of the tested kefir samples were significantly different toward Salmonella typhi (p<0.05), with the mixture of almond and cow milk notably producing better inhibition towards all tested bacteria. The above-said milk mixture also gave a better overall nutrient profile (lower fat and higher fibre). While almond milk might be a suitable substrate for kefir, it was not effectively inhibitory for all bacteria. The overall results thus conveyed the promising use of almond and cow milk mixture as an alternative substrate for kefir fermentation, further supporting its potential use as a probiotics source.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45854866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid and Blood Pressure Lowering Effects of Mikania micrantha Through En-zymatic Inhibition 薇甘菊通过酶抑制降脂降血压的作用

Journal of Tropical Life Science Pub Date : 2023-05-25 DOI: 10.11594/jtls.13.02.05

A. Ishak, Nurul Sjafie, Norhaizan Esa, H. Bahari

{"title":"Lipid and Blood Pressure Lowering Effects of Mikania micrantha Through En-zymatic Inhibition","authors":"A. Ishak, Nurul Sjafie, Norhaizan Esa, H. Bahari","doi":"10.11594/jtls.13.02.05","DOIUrl":"https://doi.org/10.11594/jtls.13.02.05","url":null,"abstract":"Mikania micrantha Kunth (Asteraceae) is a plant traditionally used to reduce the risk of hyperlipidemia and hypertension. There is limited information on the anti-hyperlipidemic and anti-hypertensive effects of the various M. micrantha leaves and stem extracts. This study aimed to examine the in vitro potential of different parts of M. micrantha (leaves and stem) extracts in inhibiting hyperlipidemia-related enzymes, i.e., pancreatic lipase (PL), lipoprotein lipase (LPL) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR), in addition to the hypertension-related, i.e., angiotensin-I converting enzyme (ACE). This study spectrophotometrically determined the inhibitory activities of hot water, cold water, 70% ethanol, and ethyl acetate M. micrantha leaves and stem extracts against the above-said enzymes using PL, LPL, HMGR, and ACE inhibition assays. The study found that the ethanol stem (ETS) extract exhibited the highest PL inhibitory activity (IC50=4.49±2.50 μg/mL), albeit the difference was insignificant (p > 0.05) compared to orlistat (IC50=0.31±0.01 μg/mL). Meanwhile, the ethanol leaves (ETL) extract yielded the highest LPL (IC50=1.42±0.48 μg/mL) and HMGR inhibitory activity (50.12±3.44%), although the greatest ACE inhibition was observed for the hot water stem (HWS) extract (97.47±1.19%). However, the result was insignificant (p > 0.05) compared to other extracts and captopril (98.42±0.93%). In brief, the extracts generally exhibited remarkable inhibitory activity against PL, LPL, HMGR, and ACE, thus conveying the M. micrantha extracts' anti-hyperlipidemic and anti-hypertensive potentials.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45439022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxic and Osteoblast Differentiation Induction Properties of Crude Polar Extract of Piper sarmentosum leaves 胡椒叶粗极性提取物的细胞毒及诱导成骨细胞分化特性

Journal of Tropical Life Science Pub Date : 2023-05-25 DOI: 10.11594/jtls.13.02.01

I. Abidin, A. Johari, Z. Ariffin, M. Yazid, H. Dyari, S. Ariffin

{"title":"Cytotoxic and Osteoblast Differentiation Induction Properties of Crude Polar Extract of Piper sarmentosum leaves","authors":"I. Abidin, A. Johari, Z. Ariffin, M. Yazid, H. Dyari, S. Ariffin","doi":"10.11594/jtls.13.02.01","DOIUrl":"https://doi.org/10.11594/jtls.13.02.01","url":null,"abstract":"Piper sarmentosum or ‘kaduk’ is a well-known herb plant in Malaysia. Its extracts were found to exhibit a bone-protective effect against osteoporotic rats. Our study aim is to morphologically observe the effect of P. sarmentosum ethyl acetate extract on the differentiation of human peripheral blood stem cells (PBSCs) into osteoblasts. P. sarmentosum extracts (1-900 µg/mL) prepared using 1% dimethyl sulfoxide (DMSO) were used in the cytotoxicity assay. Then, the differentiation assay was performed using concentrations of 1-50 µg/mL. The untreated cells acted as the negative control, while the cells cultured in 50 µg/mL ascorbic acid and 10 mM β-glycerophosphate was a positive control. The cytotoxicity effect and proliferation capacity of the cells were analyzed using Trypan Blue exclusion method, while the differentiation of PBSCs was observed using von Kossa staining and ALP gene expression analysis. The result showed a decrease in cells’ viability in a dose-dependent manner during cytotoxicity assay. After 14 days of the differentiation, a constant rate of proliferation could be observed in the treated cells and positive control, while the untreated cells showed an increase in proliferation. The mineralization of extract-treated cells showed significant differences (p<0.05) as compared to the negative control. The expression of ALP was also upregulated on day 14 of differentiation compared to day 0. In conclusion, the extract was capable to induce osteoblast differentiation of human peripheral blood stem cell and potential to be used in tissue regeneration","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48705591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modifying Effect of Anthocyanin from Purple Sweet Potatoes on Visceral Fat Tissue Inflammation and Liver Oxidative Stress in Psychological Stress-Induced Mice 紫甘薯花青素对心理应激小鼠内脏脂肪组织炎症和肝脏氧化应激的影响

Journal of Tropical Life Science Pub Date : 2023-05-25 DOI: 10.11594/jtls.13.02.18

Nia Kurnianingsih, Dian Artamevia, Antania Winarta, Andini Wulandari, Syifa Siregar, D. Hasanah, E. Widodo, Retty Ratmawati

{"title":"Modifying Effect of Anthocyanin from Purple Sweet Potatoes on Visceral Fat Tissue Inflammation and Liver Oxidative Stress in Psychological Stress-Induced Mice","authors":"Nia Kurnianingsih, Dian Artamevia, Antania Winarta, Andini Wulandari, Syifa Siregar, D. Hasanah, E. Widodo, Retty Ratmawati","doi":"10.11594/jtls.13.02.18","DOIUrl":"https://doi.org/10.11594/jtls.13.02.18","url":null,"abstract":"Psychological stress generates inflammation and oxidative stress in various tissues. Visceral fat as the target site of inflammation is then correlated with stress-induced obesity. Redox imbalance following inflammation also has prominent impacts on hepatic tissue. Therefore, the development of anti-inflammatory and antioxidant properties from plant bioactive compounds is necessary to be investigated. Anthocyanin (ANC) from various plants is previously described as a powerful anti-inflammatory and antioxidant even though its effect in psychological stress remains underexplored. Purple sweet potatoes (PSP) has ANC as its natural pigment. Previous studies revealed the potential effect of ANC from PSP on behavior and an antidepressant candidate. Thus, this study was purposed to determine the effect of ANC from PSP on inflammation and oxidative stress in visceral fat and hepatic tissue respectively. A total of 25 adult male BALB/c mice were assigned into groups of control, stress, stress+ANC 10 mg/kgBW, stress+ANC 20 mg/kgBW and stress+ANC 40 mg/kgBW. Restraint stress was applied 2 hours/day for 14 days. Enzyme-linked immunoassay (ELISA) was conducted to measure level of IL-6 and IL-10 in visceral fat as well as SOD and MDA from hepatic tissue. The results demonstrated that the supplementation of ANC reduces the level of IL-6 cytokine (p=0.005), tends to increase IL-10 (p=0.612), reduces hepatic SOD (p=0.03), and does not significantly affect the level of hepatic MDA (p=0.432). Both ratios of IL-6/IL-10 and SOD/MDA were reduced following ANC administration. Total ANC extracts are suggested to have a potential role of resisting inflammation and oxidative stress in the psychologically stressed model. Further studies are necessary to evaluate the benefits of ANC from PSP in other peripheral organs under psychological stress exposure.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47520398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of CRISPR/Cas9 Construct in Rice (Oryza sativa subsp. indica) Using Golden Gate Cloning Method Towards Drought Tolerance 应用金门克隆法构建水稻CRISPR/Cas9结构

Journal of Tropical Life Science Pub Date : 2023-05-25 DOI: 10.11594/jtls.13.02.04

A. Yusof, A. Tamizi, Nurul Zim, Siti Sattar, M. Salleh, N. Azmi, Z. Zainal, Z. Zainuddin, N. Samsulrizal

{"title":"Development of CRISPR/Cas9 Construct in Rice (Oryza sativa subsp. indica) Using Golden Gate Cloning Method Towards Drought Tolerance","authors":"A. Yusof, A. Tamizi, Nurul Zim, Siti Sattar, M. Salleh, N. Azmi, Z. Zainal, Z. Zainuddin, N. Samsulrizal","doi":"10.11594/jtls.13.02.04","DOIUrl":"https://doi.org/10.11594/jtls.13.02.04","url":null,"abstract":"Rice (Oryza sativa) is a staple food consumed by the majority of the world’s population. Climate change, however, has created a significant threat to our food security as it posed severe effects on rice production. The emergence of genome editing technology has opened a new era in crop improvement. Hence, this study aims to develop the CRISPR/Cas9 construct of drought tolerance for O. sativa subsp. indica cv. IR64 using Golden Gate cloning method. For this purpose, the generation of CRISPR/Cas9 constructs involved several stages, i.e., characterization of SUMO E2-Conjugating Enzyme (OsSCE1) gene, single-guide RNA (sgRNA) design and vector construction. FGENESH, GeneMarkS, InterProScan, and Blast2GO programmes – were used for the OsSCE1 gene characterisation. The putative OsSCE1 gene isolated from IR64 was then verified by sequencing, and the gene was 585 bp long and showed 99% identity with O. sativa on chromosome 10. In silico analysis concluded the gene is involved in abiotic stress regulation. The 20 bp sgRNA was designed manually with the aid of gRNA prediction programmes including CCTop, and Benchling. The virtual vector was validated using the Golden Gate Cloning approach and later confirmed through sequencing. The assembly involved separate vectors containing the OsSCE1 sgRNA sequence, plant selectable marker, and Cas9 cassette to construct standardised elements for hierarchical modular cloning (MoClo). This study demonstrated that our format, as the gene insertion are achievable, resulting in a speedier and more efficient assembly process which may contribute to improve drought tolerance in indica rice. Further study on the Agrobacterium-mediated transformation using the developed construct may be conducted to determine the efficacy of knocking out candidate genes in improving drought tolerance ability O. sativa","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45555586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Green Synthesis of Silver Nanoparticles Using Leaves of Chromolaena odorata and its Antioxidant Activity 用桔梗叶绿色合成纳米银及其抗氧化活性

Journal of Tropical Life Science Pub Date : 2023-05-25 DOI: 10.11594/jtls.13.02.08

Siti Hasyim, Angelle John

{"title":"Green Synthesis of Silver Nanoparticles Using Leaves of Chromolaena odorata and its Antioxidant Activity","authors":"Siti Hasyim, Angelle John","doi":"10.11594/jtls.13.02.08","DOIUrl":"https://doi.org/10.11594/jtls.13.02.08","url":null,"abstract":"Harmful chemical waste is a serious problem being faced during the synthesis of nanoparticles due to the usage of hazardous chemicals. Synthesis of silver nanoparticles using aqueous extract of the leaves of C. odorata is cheap and environmentally friendly. This study reports the synthesis of silver nanoparticles (AgNPs) using C. odorata aqueous extract as reducing agent. The leaves of C. odorata was extracted by using cold maceration technique. The phytochemicals screening of leaves was done and positive results was showed for the presence of flavonoids, tannins, saponins, and phenolics in the leaves extract of C. odorata. The synthesized C. odorata extract mediated AgNPs was characterized using several techniques including UV-Visible spectroscopy and Field Emission Scanning Electron Microscopy (FESEM). The reduction of pure Ag (I) ions to Ag (0) was monitored using UV-Vis every one hour after 24 hours up to 28 hours and it showed an absorption band at 430-450 nm. Field emission scanning electron microscopy (FESEM) was utilized to determine its particle size and the average particle size obtained was 27.3 nm. The silver nanoparticles (AgNPs) produced by using C. odorata leaves aqueous extract was determined for its antioxidant activity by using DPPH free radical scavenging assay. The IC50 value obtained was 277.29 mg/ml. Based on the results obtained, it indicates that the silver nanoparticles (AgNPs) produced using C. odorata leaves aqueous extract possessed antioxidant activity that can scavenge free radicals.","PeriodicalId":17638,"journal":{"name":"Journal of Tropical Life Science","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136345381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review on the Cytotoxic and Antimicrobial Properties of Xanthones from Cratoxylum cochinchinense 胭脂虫黄原胶的细胞毒性和抗菌性能研究进展

Journal of Tropical Life Science Pub Date : 2023-01-31 DOI: 10.11594/jtls.13.01.20

Su Lee, M. Mojulat, Grace Thangaperagasam, N. Surugau, Sheri-Ann Tan, Oliver Jhon

引用次数: 1