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Journal of Visualized Experiments最新文献

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Stromal Cell Isolation from Hematopoietic Organs 从造血器官中分离基质细胞
Journal of Visualized Experiments Pub Date : 2024-01-26 DOI: 10.3791/66231
Trine Kristiansen, Christina Mayerhofer, K. Gustafsson, David T. Scadden
{"title":"Stromal Cell Isolation from Hematopoietic Organs","authors":"Trine Kristiansen, Christina Mayerhofer, K. Gustafsson, David T. Scadden","doi":"10.3791/66231","DOIUrl":"https://doi.org/10.3791/66231","url":null,"abstract":"","PeriodicalId":17613,"journal":{"name":"Journal of Visualized Experiments","volume":"30 40","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139595364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optical Clearing and Labeling for Light-sheet Fluorescence Microscopy in Large-scale Human Brain Imaging 大规模人类大脑成像中光片荧光显微镜的光学清除和标记
Journal of Visualized Experiments Pub Date : 2024-01-26 DOI: 10.3791/65960
Danila Di Meo, Josephine Ramazzotti, M. Scardigli, Franco Cheli, Luca Pesce, N. Brady, G. Mazzamuto, I. Costantini, Francesco S. Pavone
{"title":"Optical Clearing and Labeling for Light-sheet Fluorescence Microscopy in Large-scale Human Brain Imaging","authors":"Danila Di Meo, Josephine Ramazzotti, M. Scardigli, Franco Cheli, Luca Pesce, N. Brady, G. Mazzamuto, I. Costantini, Francesco S. Pavone","doi":"10.3791/65960","DOIUrl":"https://doi.org/10.3791/65960","url":null,"abstract":"","PeriodicalId":17613,"journal":{"name":"Journal of Visualized Experiments","volume":"71 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139593552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+ T Cells During Tumorigenesis 用于评估肿瘤发生过程中抗原特异性 CD8+ T 细胞动态的引流淋巴结转移模型
Journal of Visualized Experiments Pub Date : 2024-01-26 DOI: 10.3791/65646
Yan Zhang, Xingxing Su, Lisha Wang, Zhengliang Yue, Qiao Liu, Ling Ran, Shun Lei, Jianjun Hu, Lifan Xu, Lilin Ye, Ping Ji, Guimei Li, Qizhao Huang, Shuqiong Wen
{"title":"Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+ T Cells During Tumorigenesis","authors":"Yan Zhang, Xingxing Su, Lisha Wang, Zhengliang Yue, Qiao Liu, Ling Ran, Shun Lei, Jianjun Hu, Lifan Xu, Lilin Ye, Ping Ji, Guimei Li, Qizhao Huang, Shuqiong Wen","doi":"10.3791/65646","DOIUrl":"https://doi.org/10.3791/65646","url":null,"abstract":"","PeriodicalId":17613,"journal":{"name":"Journal of Visualized Experiments","volume":"64 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139593788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model 视网膜变性小鼠模型中经瞳孔引导的巩膜下移植物移植手术
Journal of Visualized Experiments Pub Date : 2024-01-26 DOI: 10.3791/65448
Ying V. Liu, Kang V. Li, Zhuolin Li, Yuchen Lu, Minda M. McNally, Edward P. Esposito, Kanza Aziz, Mandeep S. Singh
{"title":"Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model","authors":"Ying V. Liu, Kang V. Li, Zhuolin Li, Yuchen Lu, Minda M. McNally, Edward P. Esposito, Kanza Aziz, Mandeep S. Singh","doi":"10.3791/65448","DOIUrl":"https://doi.org/10.3791/65448","url":null,"abstract":"","PeriodicalId":17613,"journal":{"name":"Journal of Visualized Experiments","volume":"1 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139593365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions 参与蛋白质-蛋白质相互作用的潜在多肽结合域氨基酸偏好的计算预测
Journal of Visualized Experiments Pub Date : 2024-01-26 DOI: 10.3791/66314
Héctor Cruz, Alejandro Llanes, Patricia L. Fernández
{"title":"Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions","authors":"Héctor Cruz, Alejandro Llanes, Patricia L. Fernández","doi":"10.3791/66314","DOIUrl":"https://doi.org/10.3791/66314","url":null,"abstract":"","PeriodicalId":17613,"journal":{"name":"Journal of Visualized Experiments","volume":"3 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139593156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hyperglycemic Clamp and Hypoglycemic Clamp in Conscious Mice 意识清醒小鼠的高血糖钳夹和低血糖钳夹
Journal of Visualized Experiments Pub Date : 2024-01-26 DOI: 10.3791/65581
Takashi Abe, Chitoku Toda
{"title":"Hyperglycemic Clamp and Hypoglycemic Clamp in Conscious Mice","authors":"Takashi Abe, Chitoku Toda","doi":"10.3791/65581","DOIUrl":"https://doi.org/10.3791/65581","url":null,"abstract":"","PeriodicalId":17613,"journal":{"name":"Journal of Visualized Experiments","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139593965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mouse Round Spermatid Injection 小鼠圆形精子注射液
Journal of Visualized Experiments Pub Date : 2024-01-26 DOI: 10.3791/65898
Haibo Zhu, Yuting Jiang, Haiwei Quan, Leyi Li, Jiarui Wei, Ruizhi Liu, Ruixue Wang
{"title":"Mouse Round Spermatid Injection","authors":"Haibo Zhu, Yuting Jiang, Haiwei Quan, Leyi Li, Jiarui Wei, Ruizhi Liu, Ruixue Wang","doi":"10.3791/65898","DOIUrl":"https://doi.org/10.3791/65898","url":null,"abstract":"","PeriodicalId":17613,"journal":{"name":"Journal of Visualized Experiments","volume":"61 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139593944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Ex Vivo Model of Ovarian Cancer Peritoneal Metastasis Using Human Omentum 利用人体网膜建立卵巢癌腹膜转移的体内外模型
Journal of Visualized Experiments Pub Date : 2024-01-26 DOI: 10.3791/66031
Terrence Wong, Roslyn Tedja, Hussein Chehade, Robert Morris, A. Alvero, Gil Mor
{"title":"An Ex Vivo Model of Ovarian Cancer Peritoneal Metastasis Using Human Omentum","authors":"Terrence Wong, Roslyn Tedja, Hussein Chehade, Robert Morris, A. Alvero, Gil Mor","doi":"10.3791/66031","DOIUrl":"https://doi.org/10.3791/66031","url":null,"abstract":"","PeriodicalId":17613,"journal":{"name":"Journal of Visualized Experiments","volume":"38 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139594244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-Throughput Behavioral Aging and Lifespan Assays Using the Lifespan Machine 使用寿命测定仪进行高通量行为老化和寿命测定
Journal of Visualized Experiments Pub Date : 2024-01-26 DOI: 10.3791/65462
Andrea Del Carmen-Fabregat, Lucia Sedlackova, Natasha Oswal, Nicholas Stroustrup
{"title":"High-Throughput Behavioral Aging and Lifespan Assays Using the Lifespan Machine","authors":"Andrea Del Carmen-Fabregat, Lucia Sedlackova, Natasha Oswal, Nicholas Stroustrup","doi":"10.3791/65462","DOIUrl":"https://doi.org/10.3791/65462","url":null,"abstract":"","PeriodicalId":17613,"journal":{"name":"Journal of Visualized Experiments","volume":"42 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139595086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiplex Cyclic Fluorescent Immunohistochemistry 多重循环荧光免疫组化技术
Journal of Visualized Experiments Pub Date : 2024-01-26 DOI: 10.3791/66136
Yun Chen, Hui Zhang, Yaodong Fan, Liu Yang, Yan Dong
{"title":"Multiplex Cyclic Fluorescent Immunohistochemistry","authors":"Yun Chen, Hui Zhang, Yaodong Fan, Liu Yang, Yan Dong","doi":"10.3791/66136","DOIUrl":"https://doi.org/10.3791/66136","url":null,"abstract":"","PeriodicalId":17613,"journal":{"name":"Journal of Visualized Experiments","volume":"74 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139593727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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