Journal of Zhejiang University. Science. B最新文献

Input-output specific orchestration of aversive valence in lateral habenula during stress dynamics. 在应激动态过程中，外侧哈文脑中厌恶情绪的输入输出特异性协调。

Journal of Zhejiang University. Science. B Pub Date : 2024-04-03 DOI: 10.1631/jzus.b2300933

Taida Huang, Xiaonan Guo, Xiaomin Huang, Chenju Yi, Yihui Cui, Yiyan Dong

{"title":"Input-output specific orchestration of aversive valence in lateral habenula during stress dynamics.","authors":"Taida Huang, Xiaonan Guo, Xiaomin Huang, Chenju Yi, Yihui Cui, Yiyan Dong","doi":"10.1631/jzus.b2300933","DOIUrl":"https://doi.org/10.1631/jzus.b2300933","url":null,"abstract":"Stress has been considered as a major risk factor for depressive disorders, triggering depression onset via inducing persistent dysfunctions in specialized brain regions and neural circuits. Among various regions across the brain, the lateral habenula (LHb) serves as a critical hub for processing aversive information during the dynamic process of stress accumulation, thus having been implicated in the pathogenesis of depression. LHb neurons integrate aversive valence conveyed by distinct upstream inputs, many of which selectively innervate the medial part (LHbM) or lateral part (LHbL) of LHb. LHb subregions also separately assign aversive valence via dissociable projections to the downstream targets in the midbrain which provides feedback loops. Despite these strides, the spatiotemporal dynamics of LHb-centric neural circuits remain elusive during the progression of depression-like state under stress. In this review, we attempt to describe a framework in which LHb orchestrates aversive valence via the input-output specific neuronal architecture. Notably, a physiological form of Hebbian plasticity in LHb under multiple stressors has been unveiled to incubate neuronal hyperactivity in an input-specific manner, which causally encodes chronic stress experience and drives depression onset. Collectively, the recent progress and future efforts in elucidating LHb circuits shed light on early interventions and circuit-specific antidepressant therapies.","PeriodicalId":17601,"journal":{"name":"Journal of Zhejiang University. Science. B","volume":"90 ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140748149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Energy deprivation-induced autophagy and aggrephagy: insights from yeast and mammals. 能量匮乏诱导的自噬和聚噬：来自酵母和哺乳动物的启示。

Journal of Zhejiang University. Science. B Pub Date : 2024-04-03 DOI: 10.1631/jzus.b2300884

Siyu Fan, Ying-Hua Chen, Weijing Yao, Cong Yi

引用次数: 0

Large-scale genome-wide SNP analysis reveals the rugged (and ragged) landscape of global ancestry, phylogeny, and demographic history in chicken breeds. 大规模全基因组 SNP 分析揭示了鸡种全球祖先、系统发育和人口历史的崎岖（和粗糙）景观。

Journal of Zhejiang University. Science. B Pub Date : 2024-04-01 DOI: 10.1631/jzus.B2300443

N. Dementieva, Yuriy S. Shcherbakov, O. Stanishevskaya, A. Vakhrameev, Tatiana A Larkina, Artem P. Dysin, Olga A Nikolaeva, Anna E. Ryabova, Anastasiia I Azovtseva, O. V. Mitrofanova, G. Peglivanyan, Natalia R Reinbach, Darren K. Griffin, Michael N. Romanov

{"title":"Large-scale genome-wide SNP analysis reveals the rugged (and ragged) landscape of global ancestry, phylogeny, and demographic history in chicken breeds.","authors":"N. Dementieva, Yuriy S. Shcherbakov, O. Stanishevskaya, A. Vakhrameev, Tatiana A Larkina, Artem P. Dysin, Olga A Nikolaeva, Anna E. Ryabova, Anastasiia I Azovtseva, O. V. Mitrofanova, G. Peglivanyan, Natalia R Reinbach, Darren K. Griffin, Michael N. Romanov","doi":"10.1631/jzus.B2300443","DOIUrl":"https://doi.org/10.1631/jzus.B2300443","url":null,"abstract":"The worldwide chicken gene pool encompasses a remarkable, but shrinking, number of divergently selected breeds of diverse origin. This study was a large-scale genome-wide analysis of the landscape of the complex molecular architecture, genetic variability, and detailed structure among 49 populations. These populations represent a significant sample of the world's chicken breeds from Europe (Russia, Czech Republic, France, Spain, UK, etc.), Asia (China), North America (USA), and Oceania (Australia). Based on the results of breed genotyping using the Illumina 60K single nucleotide polymorphism (SNP) chip, a bioinformatic analysis was carried out. This included the calculation of heterozygosity/homozygosity statistics, inbreeding coefficients, and effective population size. It also included assessment of linkage disequilibrium and construction of phylogenetic trees. Using multidimensional scaling, principal component analysis, and ADMIXTURE-assisted global ancestry analysis, we explored the genetic structure of populations and subpopulations in each breed. An overall 49-population phylogeny analysis was also performed, and a refined evolutionary model of chicken breed formation was proposed, which included egg, meat, dual-purpose types, and ambiguous breeds. Such a large-scale survey of genetic resources in poultry farming using modern genomic methods is of great interest both from the viewpoint of a general understanding of the genetics of the domestic chicken and for the further development of genomic technologies and approaches in poultry breeding. In general, whole genome SNP genotyping of promising chicken breeds from the worldwide gene pool will promote the further development of modern genomic science as applied to poultry.","PeriodicalId":17601,"journal":{"name":"Journal of Zhejiang University. Science. B","volume":"28 8","pages":"324-340"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140796889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of pancreatic exocrine insufficiency on gut microbiota. 胰腺外分泌功能不全对肠道微生物群的影响

Journal of Zhejiang University. Science. B Pub Date : 2024-04-01 DOI: 10.1631/jzus.B2300070

Yulin Guo, F. Cao, Fei Li

引用次数: 0

Nrf2-mediated ferroptosis of spermatogenic cells involved in male reproductive toxicity induced by polystyrene nanoplastics in mice. Nrf2介导的生精细胞铁凋亡参与了聚苯乙烯纳米塑料对小鼠雄性生殖毒性的诱导。

Journal of Zhejiang University. Science. B Pub Date : 2024-04-01 DOI: 10.1631/jzus.B2300138

Xufeng Fu, Hang Han, Hong Yang, Bo Xu, Wenjie Dai, Ling Liu, Tiantian He, Xing Du, Xiuying Pei

{"title":"Nrf2-mediated ferroptosis of spermatogenic cells involved in male reproductive toxicity induced by polystyrene nanoplastics in mice.","authors":"Xufeng Fu, Hang Han, Hong Yang, Bo Xu, Wenjie Dai, Ling Liu, Tiantian He, Xing Du, Xiuying Pei","doi":"10.1631/jzus.B2300138","DOIUrl":"https://doi.org/10.1631/jzus.B2300138","url":null,"abstract":"Microplastics (MPs) and nanoplastics (NPs) have become hazardous materials due to the massive amount of plastic waste and disposable masks, but their specific health effects remain uncertain. In this study, fluorescence-labeled polystyrene NPs (PS-NPs) were injected into the circulatory systems of mice to determine the distribution and potential toxic effects of NPs in vivo. Interestingly, whole-body imaging found that PS-NPs accumulated in the testes of mice. Therefore, the toxic effects of PS-NPs on the reproduction systems and the spermatocytes cell line of male mice, and their mechanisms, were investigated. After oral exposure to PS-NPs, their spermatogenesis was affected and the spermatogenic cells were damaged. The spermatocyte cell line GC-2 was exposed to PS-NPs and analyzed using RNA sequencing (RNA-seq) to determine the toxic mechanisms; a ferroptosis pathway was found after PS-NP exposure. The phenomena and indicators of ferroptosis were then determined and verified by ferroptosis inhibitor ferrostatin-1 (Fer-1), and it was also found that nuclear factor erythroid 2-related factor 2 (Nrf2) played an important role in spermatogenic cell ferroptosis induced by PS-NPs. Finally, it was confirmed in vivo that this mechanism of Nrf2 played a protective role in PS-NPs-induced male reproductive toxicity. This study demonstrated that PS-NPs induce male reproductive dysfunction in mice by causing spermatogenic cell ferroptosis dependent on Nrf2.","PeriodicalId":17601,"journal":{"name":"Journal of Zhejiang University. Science. B","volume":"41 2","pages":"307-323"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140767377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging role of lncRNAs as mechanical signaling molecules in mechanotransduction and their association with Hippo-YAP signaling: a review. lncRNA作为机械信号分子在机械传导中的新作用及其与Hippo-YAP信号传导的关联：综述。

Journal of Zhejiang University. Science. B Pub Date : 2024-04-01 DOI: 10.1631/jzus.B2300497

Siyi Lin, Xin He, Ying Wang, Yu Chen, Aifu Lin

{"title":"Emerging role of lncRNAs as mechanical signaling molecules in mechanotransduction and their association with Hippo-YAP signaling: a review.","authors":"Siyi Lin, Xin He, Ying Wang, Yu Chen, Aifu Lin","doi":"10.1631/jzus.B2300497","DOIUrl":"https://doi.org/10.1631/jzus.B2300497","url":null,"abstract":"Cells within tissues are subject to various mechanical forces, including hydrostatic pressure, shear stress, compression, and tension. These mechanical stimuli can be converted into biochemical signals through mechanoreceptors or cytoskeleton-dependent response processes, shaping the microenvironment and maintaining cellular physiological balance. Several studies have demonstrated the roles of Yes-associated protein (YAP) and its homolog transcriptional coactivator with PDZ-binding motif (TAZ) as mechanotransducers, exerting dynamic influence on cellular phenotypes including differentiation and disease pathogenesis. This regulatory function entails the involvement of the cytoskeleton, nucleoskeleton, integrin, focal adhesions (FAs), and the integration of multiple signaling pathways, including extracellular signal-regulated kinase (ERK), wingless/integrated (WNT), and Hippo signaling. Furthermore, emerging evidence substantiates the implication of long non-coding RNAs (lncRNAs) as mechanosensitive molecules in cellular mechanotransduction. In this review, we discuss the mechanisms through which YAP/TAZ and lncRNAs serve as effectors in responding to mechanical stimuli. Additionally, we summarize and elaborate on the crucial signal molecules involved in mechanotransduction.","PeriodicalId":17601,"journal":{"name":"Journal of Zhejiang University. Science. B","volume":"331 ","pages":"280-292"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140763695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the growth potential: harnessing the power of synbiotics to enhance cultivation of Pleurotus spp. 释放生长潜能：利用合成生物素的力量来提高刺五加属植物的栽培。

Journal of Zhejiang University. Science. B Pub Date : 2024-04-01 DOI: 10.1631/jzus.B2300383

R. Patil, A. N. M. Ramli, Ang Shu Xuan, Ng Zhi Xin, N. I. W. Azelee, Prakash Bhuyar

{"title":"Unlocking the growth potential: harnessing the power of synbiotics to enhance cultivation of Pleurotus spp.","authors":"R. Patil, A. N. M. Ramli, Ang Shu Xuan, Ng Zhi Xin, N. I. W. Azelee, Prakash Bhuyar","doi":"10.1631/jzus.B2300383","DOIUrl":"https://doi.org/10.1631/jzus.B2300383","url":null,"abstract":"The oyster mushroom (Pleurotus spp.) is one of the most widely cultivated mushroom species globally. The present study investigated the effect of synbiotics on the growth and quality of Pleurotus ostreatus and Pleurotus pulmonarius. Different synbiotics formulations were applied by spraying mushroom samples daily and measuring their growth parameters, yield, biological efficiency, proximate composition, mineral content, total phenolic content (TPC), and diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity. Results demonstrated that the most significant yield of oyster mushrooms was harvested from synbiotics sprayed with inulin and Lactobacillus casei (56.92 g). Likewise, the highest biological efficiency obtained with a similar synbiotic was 12.65%. Combining inulin and L. casei was the most effective method of improving the mushrooms' growth performance and nutrient content in both samples. Furthermore, synbiotics that combined inulin and L. casei resulted in the highest TPC (20.550 mg gallic acid equivalent (GAE)/g dry extract (DE)) in white oyster mushrooms (P. ostreatus). In comparison, in grey mushroom (P. pulmonarius) the highest TPC was yielded by L. casei (1.098 mg GAE/g DE) followed by inulin and L. casei (1.079 mg GAE/g DE). The DPPH results indicated that the oyster mushroom could be an efficient antioxidant. The results revealed that applying synbiotics improved the mushrooms' quality by increasing their antioxidant capacity with higher amounts of phenolic compounds and offering better health benefits with the increased levels of mineral elements. Together, these studies demonstrated the potential of using synbiotics as a biofertilizer, which is helpful for mushroom cultivation; therefore, it might solve the challenge of inconsistent quality mushroom growers face.","PeriodicalId":17601,"journal":{"name":"Journal of Zhejiang University. Science. B","volume":"336 2","pages":"293-306"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140781951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination. LAMP-CRISPR/Cas12b与热不稳定的尿嘧啶- dna糖基化酶联合快速检测副溶血性弧菌，消除携带性污染。

Journal of Zhejiang University. Science. B Pub Date : 2023-08-15 DOI: 10.1631/jzus.B2200705

Fang Wu, Chen Lu, Wenhao Hu, Xin Guo, Jiayue Chen, Zhidan Luo

引用次数: 0

MRI-derived radiomics models for diagnosis, aggressiveness, and prognosis evaluation in prostate cancer. mri衍生放射组学模型用于前列腺癌的诊断、侵袭性和预后评估。

Journal of Zhejiang University. Science. B Pub Date : 2023-08-15 DOI: 10.1631/jzus.B2200619

Xuehua Zhu, Lizhi Shao, Zhenyu Liu, Zenan Liu, Jide He, Jiangang Liu, Hao Ping, Jian Lu

{"title":"MRI-derived radiomics models for diagnosis, aggressiveness, and prognosis evaluation in prostate cancer.","authors":"Xuehua Zhu, Lizhi Shao, Zhenyu Liu, Zenan Liu, Jide He, Jiangang Liu, Hao Ping, Jian Lu","doi":"10.1631/jzus.B2200619","DOIUrl":"https://doi.org/10.1631/jzus.B2200619","url":null,"abstract":"Prostate cancer (PCa) is a pernicious tumor with high heterogeneity, which creates a conundrum for making a precise diagnosis and choosing an optimal treatment approach. Multiparametric magnetic resonance imaging (mp-MRI) with anatomical and functional sequences has evolved as a routine and significant paradigm for the detection and characterization of PCa. Moreover, using radiomics to extract quantitative data has emerged as a promising field due to the rapid growth of artificial intelligence (AI) and image data processing. Radiomics acquires novel imaging biomarkers by extracting imaging signatures and establishes models for precise evaluation. Radiomics models provide a reliable and noninvasive alternative to aid in precision medicine, demonstrating advantages over traditional models based on clinicopathological parameters. The purpose of this review is to provide an overview of related studies of radiomics in PCa, specifically around the development and validation of radiomics models using MRI-derived image features. The current landscape of the literature, focusing mainly on PCa detection, aggressiveness, and prognosis evaluation, is reviewed and summarized. Rather than studies that exclusively focus on image biomarker identification and method optimization, models with high potential for universal clinical implementation are identified. Furthermore, we delve deeper into the critical concerns that can be addressed by different models and the obstacles that may arise in a clinical scenario. This review will encourage researchers to design models based on actual clinical needs, as well as assist urologists in gaining a better understanding of the promising results yielded by radiomics.","PeriodicalId":17601,"journal":{"name":"Journal of Zhejiang University. Science. B","volume":"24 8","pages":"663-681"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423970/pdf/JZhejiangUnivSciB-24-8-663.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10059936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactobacillus gasseri LA39 promotes hepatic primary bile acid biosynthesis and intestinal secondary bile acid biotransformation. 乳酸杆菌LA39促进肝脏初级胆汁酸的生物合成和肠道次级胆汁酸的生物转化。

Journal of Zhejiang University. Science. B Pub Date : 2023-08-15 DOI: 10.1631/jzus.B2200439

Jun Hu, Qiliang Hou, Wenyong Zheng, Tao Yang, Xianghua Yan

{"title":"Lactobacillus gasseri LA39 promotes hepatic primary bile acid biosynthesis and intestinal secondary bile acid biotransformation.","authors":"Jun Hu, Qiliang Hou, Wenyong Zheng, Tao Yang, Xianghua Yan","doi":"10.1631/jzus.B2200439","DOIUrl":"https://doi.org/10.1631/jzus.B2200439","url":null,"abstract":"A growing body of evidence has linked the gut microbiota to liver metabolism. The manipulation of intestinal microflora has been considered as a promising avenue to promote liver health. However, the effects of Lactobacillus gasseri LA39, a potential probiotic, on liver metabolism remain unclear. Accumulating studies have investigated the proteomic profile for mining the host biological events affected by microbes, and used the germ-free (GF) mouse model to evaluate host-microbe interaction. Here, we explored the effects of L. gasseri LA39 gavage on the protein expression profiles of the liver of GF mice. Our results showed that a total of 128 proteins were upregulated, whereas a total of 123 proteins were downregulated by treatment with L. gasseri LA39. Further bioinformatics analyses suggested that the primary bile acid (BA) biosynthesis pathway in the liver was activated by L. gasseri LA39. Three differentially expressed proteins (cytochrome P450 family 27 subfamily A member 1 (CYP27A1), cytochrome P450 family 7 subfamily B member 1 (CYP7B1), and cytochrome P450 family 8 subfamily B member 1 (CYP8B1)) involved in the primary BA biosynthesis pathway were further validated by western blot assay. In addition, targeted metabolomic analyses demonstrated that serum and fecal β‍-muricholic acid (a primary BA), dehydrolithocholic acid (a secondary BA), and glycolithocholic acid-3-sulfate (a secondary BA) were significantly increased by L. gasseri LA39. Thus, our data revealed that L. gasseri LA39 activates the hepatic primary BA biosynthesis and promotes the intestinal secondary BA biotransformation. Based on these findings, we suggest that L. gasseri LA39 confers an important function in the gut‒liver axis through regulating BA metabolism.","PeriodicalId":17601,"journal":{"name":"Journal of Zhejiang University. Science. B","volume":"24 8","pages":"734-748"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423968/pdf/JZhejiangUnivSciB-24-8-734.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10005730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0