Journal of ultrastructure research最新文献_第2页

Quantitative analysis of development of mitochondrial ultrastructure in differentiating mouse hepatocytes during postnatal period 产后分化小鼠肝细胞线粒体超微结构发育的定量分析

Journal of ultrastructure research Pub Date : 1985-12-01 Epub Date: 2004-12-06 DOI: 10.1016/0889-1605(85)90099-0

Shinsuke Kanamura, Kazuo Kanai, Motoko Oka, Yoshihiko Shugyo, Jun Watanabe

{"title":"Quantitative analysis of development of mitochondrial ultrastructure in differentiating mouse hepatocytes during postnatal period","authors":"Shinsuke Kanamura, Kazuo Kanai, Motoko Oka, Yoshihiko Shugyo, Jun Watanabe","doi":"10.1016/0889-1605(85)90099-0","DOIUrl":"10.1016/0889-1605(85)90099-0","url":null,"abstract":"<div><p>Between birth and 10 days of age, the volume density (volume/unit cytoplasmic volume) of the matrix, and the surface density (area/unit cytoplasmic volume) of the inner membrane and cristae increased in both periportal and perihepatic hepatocytes, and did not differ significantly between the cells of the two zones. After 10 days of age, however, the volume density of the matrix decreased in perihepatic cells and remained unchanged in periportal cells, and, therefore, it became greater in periportal cells than in perihepatic cells in 20-day-old and adult animals. The surface density of the inner membrane and cristae decreased in the cells of both zones. Further, the hepatocyte volume increased markedly, especially in perihepatic zones between 20 days of age and the adult. The results show that, in postnatally differentiating hepatocytes, mitochondria are likely to develop during early postnatal period, then the structural heterogeneity of mitochondria arises, and hepatocyte volume increases markedly during late postnatal period after weaning. Thus, the process of postnatal hepatocyte differentiation includes such several phases of development.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"93 3","pages":"Pages 195-204"},"PeriodicalIF":0.0,"publicationDate":"1985-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90099-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14952897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Author index for volume 93 第93卷的作者索引

Journal of ultrastructure research Pub Date : 1985-12-01 Epub Date: 2004-12-06 DOI: 10.1016/0889-1605(85)90100-4

引用次数: 0

Cumulative subject index for volumes 90–93 第90卷至第93卷的累积主题索引

Journal of ultrastructure research Pub Date : 1985-12-01 Epub Date: 2004-12-06 DOI: 10.1016/0889-1605(85)90101-6

引用次数: 0

Ultrastructure of reconstituted rat liver microsomal membranes and cytochromeb5- orP-450-containing proteoliposomes 重组大鼠肝微粒体膜和含细胞色素5- orp -450蛋白脂质体的超微结构

Journal of ultrastructure research Pub Date : 1985-10-01 Epub Date: 2004-12-01 DOI: 10.1016/0889-1605(85)90085-0

V.L. Borovyagin , Yu.S. Tarachovsky , I.P. Kanaeva , A.V. Karyakin , G.I. Bachmanova , A.I. Archakov

{"title":"Ultrastructure of reconstituted rat liver microsomal membranes and cytochromeb5- orP-450-containing proteoliposomes","authors":"V.L. Borovyagin , Yu.S. Tarachovsky , I.P. Kanaeva , A.V. Karyakin , G.I. Bachmanova , A.I. Archakov","doi":"10.1016/0889-1605(85)90085-0","DOIUrl":"10.1016/0889-1605(85)90085-0","url":null,"abstract":"<div><p>Thin sectioning and freeze-fracture electron microscopy have been used to show that it is possible to obtain topologically closed vesicles by means of reconstitution of rat liver microsomal membrane “ghosts.” The reconstitution by 15 hr dialysis resulted in the formation of vesicles with intra-membrane particles (IMP) while after 40 hr dialysis no IMP were observed in the membranes. The protein/lipid ratio and functional activity of NADPH- and NADH-linked enzyme systems were similar in both cases. Cytochrome<em>P</em>-450 (LM<sub>2</sub>) was incorporated into liposomes of different composition (protein : lipid ratio — 1:200). IMP were observed only when the incorporation of cytochrome P-450 was performed in the presence of detergent Emulgen 913 as specific additive to the initial protein-lipid-sodium cholate mixture or in the course of incubation of proteoliposomal suspensions at 37°C. After the incorporation of cytochrome<em>b<sub>5</sub></em> into azolectin liposomes vesicular membranes contain IMP if the incorporated membrane protein: lipid ratio is at least 1:50. Pronase-induced splitting off of a 11 kDa heme-containing fragment of cytochrome<em>b<sub>5</sub></em> did not affect IMP content. The conditions of IMP formation in reconstituted membranes and in microsomal ghosts are discussed.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"93 1","pages":"Pages 50-60"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90085-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14950733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Localization of potential Ca2+ binding sites in lily pollen tubes and maize calyptra cells: Transmission electron microscopy, proton microprobe analysis and electron spectroscopic imaging 百合花粉管和玉米杯状体细胞中潜在Ca2+结合位点的定位：透射电子显微镜、质子探针分析和电子光谱成像

Journal of ultrastructure research Pub Date : 1985-10-01 Epub Date: 2004-12-01 DOI: 10.1016/0889-1605(85)90087-4

Werner Herth , Hans-Dieter Reiss , Bruno Hertler , Richard Bauer , Kurt Traxel , Christoph Ender

引用次数: 12

Ein beitrag zur frage welche kra¨fte und faktoren du¨nnstschneiden bewirken 对哪些力和因素导致切割问题的贡献

Journal of ultrastructure research Pub Date : 1985-10-01 Epub Date: 2004-12-01 DOI: 10.1016/0889-1605(85)90090-4

Karl G. Lickfeld

{"title":"Ein beitrag zur frage welche kra¨fte und faktoren du¨nnstschneiden bewirken","authors":"Karl G. Lickfeld","doi":"10.1016/0889-1605(85)90090-4","DOIUrl":"https://doi.org/10.1016/0889-1605(85)90090-4","url":null,"abstract":"<div><p>The mechanical processes occurring around a cutting edge when a thin slice is formed are related to those effective when metal or plastic is lathed. Critically assigning to thin slicing of resins the force vectors proved or assumed to be true for shapping by chipping, the attempt is made to clarify qualitatively and quantitatively the complex interplay after having measured the effective weight force and the order of magnitude of the radius of cutting edges of glass and diamond knives. Practically, only five force vectors in a polygon determine shearing and shortening of chips or thin slices, shortening obeying a broken rational function. By computing the relative values of shortening, all other force vectors can be computed. The results obtained for the normal force on the edge surface and the tangential force in this surface shed light on the effect of systematical variation of setting angle, bevel angle, and chip angle for thin slicing; thus possibilities of improvement in conventional “ultra” microtomy and in unadulterated cryotomy are shown in concluding recommendations.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"93 1","pages":"Pages 101-115"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90090-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72263502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Plasma membrane “Rosettes” in carrot and sycamore suspension culture cells 胡萝卜和梧桐悬浮培养细胞的质膜“莲座”

Journal of ultrastructure research Pub Date : 1985-10-01 Epub Date: 2004-12-01 DOI: 10.1016/0889-1605(85)90088-6

Russell L. Chapman , L. Andrew Staehelin

引用次数: 12

Novel crystalline sheets of Na,K-ATPase induced by phospholipase A2 磷脂酶A2诱导的新型Na, k - atp酶晶体片

Journal of ultrastructure research Pub Date : 1985-10-01 Epub Date: 2004-12-01 DOI: 10.1016/0889-1605(85)90081-3

Manijeh Mohraz, Movien Yee, P.R. Smith

{"title":"Novel crystalline sheets of Na,K-ATPase induced by phospholipase A2","authors":"Manijeh Mohraz, Movien Yee, P.R. Smith","doi":"10.1016/0889-1605(85)90081-3","DOIUrl":"10.1016/0889-1605(85)90081-3","url":null,"abstract":"<div><p>Treatment of purified preparations of Na,K-ATPase by phospholipase A<sub>2</sub> has led to the formation of two-dimensional crystals of the protein. Control tests with another phospholipase and two detergents have shown that crystallization occurs as the result of hydrolysis and/or solubilization of the phospholipids in the enzyme vesicles. Experimentation with various buffer systems has indicated that reduction in the amount of phospholipids alone is sufficient for inducing the formation of crystalline sheets. Inclusion of crystal inducing ions in the buffer facilitates the crystallization process, resulting in more extensive arrays. The new crystalline sheets are exclusively dimeric with average unit cell dimensions:<em>a</em> = 15.8 ± 0.4nm, <em>b</em> = 4.9 ± 0.2nm, and <em>γ</em> = 64 ± 3°. Examination of the micrographs shows that the initial intermolecular interaction leading to the formation of sheets is between the α subunits. Results from this study suggest that removal and/or modification of phospholipids by phospholipases could prove successful in crystallizing those membrane proteins in which excess lipid is the main barrier to the formation of two-dimensional arrays.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"93 1","pages":"Pages 17-26"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90081-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14145563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Promotion of MAP/MAP interaction by taxol 紫杉醇促进MAP/MAP相互作用

Journal of ultrastructure research Pub Date : 1985-10-01 Epub Date: 2004-12-01 DOI: 10.1016/0889-1605(85)90083-7

Roland Foisner, Gerhard Wiche

{"title":"Promotion of MAP/MAP interaction by taxol","authors":"Roland Foisner, Gerhard Wiche","doi":"10.1016/0889-1605(85)90083-7","DOIUrl":"10.1016/0889-1605(85)90083-7","url":null,"abstract":"<div><p>The effects of taxol on microtubule-associated proteins of high molecular weight (MAPs) were studied<em>in vitro</em>. After negative staining, microtubules reconstituted in the presence of taxol from preparations of partially purified tubulin and MAPs, besides bundled, displayed prominent elongated or globular extensions without apparent regularity. These extensions, but not the tubulin polymer, were heavily decorated after immuno-gold-labeling using antibodies to MAP-1 and MAP-2. Microtubules reconstituted in the absence of taxol showed a much more regular, and apparently helical, arrangement of MAPs along their surfaces. The formation of polymeric structures was also observed when preparation of MAPs free of tubulin were incubated with taxol. In this case in addition to large network-type aggregates with little apparent substructure, more regular structures seemingly consisting of approximately 5-nm-thick filaments arrayed in parallel were observed. Taxol-induced MAP aggregation occurred rapidly and was directly proportional to the concentration of protein, as revealed by optical density measurements. It is concluded that taxol, aside from promoting the assembly of tubulin and stabilizing microtubules, promotes MAP/MAP interaction.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"93 1","pages":"Pages 33-41"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90083-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14009848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Concentrically arranged endoplasmic reticulum containing some lamellae (bar-like structure) in alveolar type II cells of rat lung 大鼠肺ⅱ型肺泡细胞内集中排列的内质网，内质网中含有一些小片(棒状结构)

Journal of ultrastructure research Pub Date : 1985-10-01 Epub Date: 2004-12-01 DOI: 10.1016/0889-1605(85)90091-6

Sanae Shimura, Tohru Aoki, Motoaki Tomioka, Yuriko Shindoh, Tamotsu Takishima

{"title":"Concentrically arranged endoplasmic reticulum containing some lamellae (bar-like structure) in alveolar type II cells of rat lung","authors":"Sanae Shimura, Tohru Aoki, Motoaki Tomioka, Yuriko Shindoh, Tamotsu Takishima","doi":"10.1016/0889-1605(85)90091-6","DOIUrl":"10.1016/0889-1605(85)90091-6","url":null,"abstract":"<div><p>We examined<em>in vivo</em> the effect of pilocarpine (a cholinergic agent) and cycloheximide (an inhibitor of protein synthesis) on the “bar-like structures” in alveolar type II cells of rat lung to clarify their origin and significance in pulmonary surfactant production and secretion. Lungs were examined with an electron microscope using ultrathin sectioning, freeze-fracture technique, and morphometry. The bar-like structures in type II cells consisted of a concentrically arrangeed endoplasmic reticulum containing some amount of osmiophilic periodic material similar to the lamellae of lamellar bodies. Pilocarpine induced the accumulation of lamellar bodies of normal size which paralleled the increase in the number of bar-like structures in the cytoplasm of the type II cells. Cycloheximide induced a decrease in size of the lamellar bodies and an enlargement of the bar-like structures. Our morphological findings suggest that: (1) The phospholipid that would normally be incorporated into the lamellar bodies might be sequested instead in the concentrically arranged endoplasmic reticulum, forming the bar-like structures, and (2) The enlargement and the increased number of bar-like structures may be responsible in part for the changed metabolic process of surfactant production by alveolar type II cells.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"93 1","pages":"Pages 116-128"},"PeriodicalIF":0.0,"publicationDate":"1985-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90091-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14959156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8