{"title":"Activity of Lycii fructus against Colorectal Cancer Based on Network Pharmacology and in Vitro Experiments","authors":"娇秀 樊","doi":"10.12677/md.2023.133047","DOIUrl":"https://doi.org/10.12677/md.2023.133047","url":null,"abstract":"Objective: To study the activity and the molecular mechanism of the active ingredients from Lycii fructus against colorectal cancer based on network pharmacology and in vitro experiments. Me-thods: The active components and corresponding targets of Lycii fructus were predicted by TCMSP; screening colorectal cancer related targets by TTD database, Gene Card database, and DisGeNET database; the common targets of Lycii fructus and colorectal cancer were obtained using Venny2.1 online tool, and PPI (Protein-Protein Interaction) networks analysis was conducted using STRING database; Gene Ontology (GO) enrichment and KEGG pathway enrichment were analyzed by Me-tascape website; MTT assay was used to examine the anticancer activity of the extract from Lycii fructus against colorectal cancer. Results: 35 active ingredients of Lycii fructus were predicted, with 121 potential anti-cancer targets identified. The 5 most promising targets based on PPI analysis were TP53, HSP90AA1, AKT1, MAPK1, and RELA. KEGG enrichment showed that the active ingredients from Lycii fructus mainly functioned via PI3K-Akt signaling pathway, HIF-1 signaling pathway, and relaxin signaling pathway. MTT assay was used to examine the effect of the extract from Lycii fructus on the colorectal cancer cell viability and its antioxidant activity. Conclusion: Based on the network pharmacology and in vitro experiments, the possible antitumor targets of the active ingredients from Lycii fructus were screened and their antitumor activity was verified in vitro , providing a reference for the further study of the anticancer mechanism of Lycii fructus .","PeriodicalId":17544,"journal":{"name":"Journal of Veterinary Science & Medical Diagnosis","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86472729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study on Expression of sST2 and IL-33 in Vascular Calcification of Maintenance Hemodialysis Patients","authors":"佳武 李","doi":"10.12677/md.2023.131003","DOIUrl":"https://doi.org/10.12677/md.2023.131003","url":null,"abstract":"","PeriodicalId":17544,"journal":{"name":"Journal of Veterinary Science & Medical Diagnosis","volume":"371 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77485328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Progress in Diagnosis and Treatment of Ankylosing Spondylitis","authors":"春丽 卢","doi":"10.12677/md.2023.132019","DOIUrl":"https://doi.org/10.12677/md.2023.132019","url":null,"abstract":"","PeriodicalId":17544,"journal":{"name":"Journal of Veterinary Science & Medical Diagnosis","volume":" 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91549430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Progress in the Study of Episiotomy and Its Complications","authors":"李 高","doi":"10.12677/md.2023.133048","DOIUrl":"https://doi.org/10.12677/md.2023.133048","url":null,"abstract":"Childbirth always puts traumatic stress on a woman’s body. To reduce perineal trauma during childbirth, obstetricians use an episiotomy. Despite controversy over its use, episiotomy remains a common practice. Weighing the pros and cons, the scientific literature supports the selective use of episiotomy. With the worldwide trend of decreasing use of episiotomy, several techniques have","PeriodicalId":17544,"journal":{"name":"Journal of Veterinary Science & Medical Diagnosis","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76162633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diagnostic Features of Pituitary Stalk Block-ade Syndrome and Review of the Literature","authors":"荣荣 王","doi":"10.12677/md.2023.132029","DOIUrl":"https://doi.org/10.12677/md.2023.132029","url":null,"abstract":"","PeriodicalId":17544,"journal":{"name":"Journal of Veterinary Science & Medical Diagnosis","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74669042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research Progress of PCSK9 Function in Tu-mor Microenvironment of Breast Cancer","authors":"玉东 慕","doi":"10.12677/md.2023.131008","DOIUrl":"https://doi.org/10.12677/md.2023.131008","url":null,"abstract":"","PeriodicalId":17544,"journal":{"name":"Journal of Veterinary Science & Medical Diagnosis","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91191074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Segmentation Scheme for Ultrasound Images of Thyroid Nodules","authors":"乘麟 赵","doi":"10.12677/md.2023.132025","DOIUrl":"https://doi.org/10.12677/md.2023.132025","url":null,"abstract":"","PeriodicalId":17544,"journal":{"name":"Journal of Veterinary Science & Medical Diagnosis","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84689221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study on the Relationship between Hypothyroidism during Pregnancy and Gestational Diabetes Mellitus","authors":"可婧 李","doi":"10.12677/md.2023.133042","DOIUrl":"https://doi.org/10.12677/md.2023.133042","url":null,"abstract":"In recent years, the relationship between gestational hypothyroidism and gestational diabetes has attracted widespread attention. Studies have found that the former can cause insulin","PeriodicalId":17544,"journal":{"name":"Journal of Veterinary Science & Medical Diagnosis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84749554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Clinical Observation on the Treatment of Silicosis Remission Stage (Spleen and Kidney Qi Deficiency Syndrome) with Self Drawn Experience Formula for Strengthening the Spleen, Tonifying the Kidney, and Clearing the Lung","authors":"丽娜 陈","doi":"10.12677/md.2023.132037","DOIUrl":"https://doi.org/10.12677/md.2023.132037","url":null,"abstract":"","PeriodicalId":17544,"journal":{"name":"Journal of Veterinary Science & Medical Diagnosis","volume":"280 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86738091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}