Journal of Tissue Science and Engineering最新文献_第6页

Yielding and Growing of Adipose Stem Cell Harvested from the Superficial and Deep Abdominal Subcutaneous Fat: A Case 从腹部浅层和深层皮下脂肪中获取的脂肪干细胞的生成和生长:一个案例

Journal of Tissue Science and Engineering Pub Date : 2016-11-26 DOI: 10.4172/2157-7552.1000185

Daegu Son

引用次数: 1

Immunopathology of Apical Periodontitis and Refractory Cases 根尖牙周炎及难治性病例的免疫病理分析

Journal of Tissue Science and Engineering Pub Date : 2016-11-17 DOI: 10.4172/2157-7552.1000184

E. Matsuzaki, H. Anan, Noriyoshi Matsumoto, J. Hatakeyama, M. Minakami, T. Izumi

{"title":"Immunopathology of Apical Periodontitis and Refractory Cases","authors":"E. Matsuzaki, H. Anan, Noriyoshi Matsumoto, J. Hatakeyama, M. Minakami, T. Izumi","doi":"10.4172/2157-7552.1000184","DOIUrl":"https://doi.org/10.4172/2157-7552.1000184","url":null,"abstract":"Apical periodontitis is a relatively frequently encountered disease in clinical dentistry; however, its pathogenesis and etiology are not easily elucidated. Therefore, it is not always cured, even when carefully following the highest standards of treatment and intractable apical periodontitis may occur. In addition, in long-term root canal treatment of difficult cases with intractable pain, there may be misunderstandings between the dentist and patient. While acute pain is an indispensable symptom in detecting lesions and disease, sustained chronic pain can decrease an individual’s quality of life with various negative outcomes, including decreased motivation to work. Therefore, endodontic treatments and pain control measures for a diseased tooth in intractable apical periodontitis must be developed. This review outlines the progression from the onset of the lesion and examines the immunology of apical periodontitis based on studies of model animals, indicating that interleukin-1β is a key factor in elucidating the disease state and is expected to lead to the development of an effective treatment for refractory cases.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82686803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Improvement of Gene Expression Studies in the DimethylnitrosamineInduced Liver Fibrosis Model in the Rat Using Selected Reference Genesfor Quantitative Real Time-PCR Analysis 二甲基亚硝胺诱导大鼠肝纤维化模型中基因表达的改进，选择内参基因进行实时定量pcr分析

Journal of Tissue Science and Engineering Pub Date : 2016-11-10 DOI: 10.4172/2157-7552.1000183

D. Rajendran, Gary Phang Siew Siang, Alden Toh Han Hui, K. Chooi

{"title":"Improvement of Gene Expression Studies in the DimethylnitrosamineInduced Liver Fibrosis Model in the Rat Using Selected Reference Genesfor Quantitative Real Time-PCR Analysis","authors":"D. Rajendran, Gary Phang Siew Siang, Alden Toh Han Hui, K. Chooi","doi":"10.4172/2157-7552.1000183","DOIUrl":"https://doi.org/10.4172/2157-7552.1000183","url":null,"abstract":"Background: Liver fibrosis is a reaction to chronic liver injury characterized by excessive accumulation of collagen. Due to their importance as biomarkers, the changes in gene expression in the liver during the development of fibrosis and its subsequent outcomes of cirrhosis, neoplasia or resolution are intensely studied. Quantitative realtime PCR (qPCR) with its ability to detect and measure minute amounts of nucleic acids have been increasingly used in these studies. In qPCR, the quantitation of mRNA is relative and the accuracy of results dependent on the reference genes used for standardization. However, many genes studied are normalized against single reference genes, usually housekeeping genes, without adequate justification. \u0000Methods: For the dimethylnitrosamine (DMN) induced liver fibrosis rat model, we tested 8 commonly used candidate genes (Actb, Alb, Sdha, B2m, Rn18s, Hprt1, Ppia and Gapdh) to determine their suitability as reference genes. qPCR results were analysed using four commonly used programs; NormFinder, GeNorm, Comparative ΔCt methods and BestKeeper. \u0000Result: It was determined that Gapdh and B2m were the most stable genes in normal liver. However, in DMN treated livers, Gapdh and Ppia were the most stably expressed reference genes. We validated these reference genes by using them to normalize the expression of four genes; Tgfb 1, Col1a1, Col3a1 and Tnf known to be highly expressed in liver fibrosis. \u0000Conclusion: Gapdh and Ppia are the most suitable reference genes for the normalization of qPCR data in gene expression studies of the liver in the DMN induced liver fibrosis model in the rat. We advise against the use of Actb in this experimental setting because of its low expression stability.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"173 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77495305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Oriented Microstructure in Neural Tissue Engineering: A Review 神经组织工程中的定向微结构研究进展

Journal of Tissue Science and Engineering Pub Date : 2016-11-10 DOI: 10.4172/2157-7552.1000182

F. Ghorbani, A. Zamanian

引用次数: 8

Human Tissue Engineering: A Relevant Model for Identifying microRNAs Activated by Glioblastoma/Neural Tissue Interaction 人体组织工程:鉴定胶质母细胞瘤/神经组织相互作用激活的microrna的相关模型

Journal of Tissue Science and Engineering Pub Date : 2016-10-29 DOI: 10.4172/2157-7552.1000180

É. Cosset

引用次数: 1

Actovegin equals performance enhancing drug doping: fact or fiction activegin等同于兴奋剂:事实还是虚构

Journal of Tissue Science and Engineering Pub Date : 2016-10-23 DOI: 10.4172/2157-7552.1000179

P. Lee, A. Kwan, Paul M. Smith, James P. Brock, L. Nokes

引用次数: 3

Cell culture techniques essential for toxicity testing of inhaled materials and nanomaterials in vitro 细胞培养技术对吸入材料和纳米材料的体外毒性测试至关重要

Journal of Tissue Science and Engineering Pub Date : 2016-10-05 DOI: 10.4172/2157-7552.1000181

S. Bakand

引用次数: 10

Current Status of Adipose-Derived Mesenchymal Stem Cells in Cartilage Repair 脂肪来源间充质干细胞在软骨修复中的现状

Journal of Tissue Science and Engineering Pub Date : 2016-09-30 DOI: 10.4172/2157-7552.1000177

Arianna L. Gianakos, J. Kennedy

引用次数: 1

Cell Sheet Technology using Human Umbilical Cord Mesenchymal Stem Cells for Myocardial Tissue Engineering 利用人脐带间充质干细胞进行心肌组织工程的细胞片技术

Journal of Tissue Science and Engineering Pub Date : 2016-09-11 DOI: 10.4172/2157-7552.1000178

Lakshmi R Nair, T. Kumary

{"title":"Cell Sheet Technology using Human Umbilical Cord Mesenchymal Stem Cells for Myocardial Tissue Engineering","authors":"Lakshmi R Nair, T. Kumary","doi":"10.4172/2157-7552.1000178","DOIUrl":"https://doi.org/10.4172/2157-7552.1000178","url":null,"abstract":"With limited regenerative capacity and most complex structural and electrophysiological properties; recapitulating the cardiac tissue is a challenging task for the researchers. The cell injection was found unreliable due to the cell loss and low retention of the transplanted cells. This could be overcome by the technique of cell sheet engineering. Scaffold free, thick, cell dense, three dimensional constructs could be generated for suture free transplantation. For the generation of the cardiac constructs the neonatal cardiomyocytes and myoblasts were mostly used. They were tedious to isolate and culture and the risk of arrhythmogenic foci prevailed. Hence the concept of differentiating a suitable allogeneic cell source to myocardial lineage seemed relevant. Human umbilical cord mesenchymal stem cells (hUCMSCs) are emerging with the assistance of differentiating agents and cell sheet engineering for addressing the cardiac regeneration. The preliminary report on this regard has been published. The cells attained cardiomyocyte-like morphology with the expression of alpha-actinin and myosin heavy chain on culturing with cardiac conditioned medium and the inducer sphingosine-1-phosphate. It presented cardiomyocyte-like action potential and voltage gated currents. Hence the cell sheet engineering approach with cells differentiated to cardiac lineage using specific agents is a recent area to be explored.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"65 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2016-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75251303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Efficient Plant Regeneration of Selected Kenyan Sweetpotato (Ipomoea batatas (L.) Lam.) Cultivars through Somatic Embryogenesis 肯尼亚甘薯的高效植株再生研究Lam)。通过体细胞胚胎发生的品种

Journal of Tissue Science and Engineering Pub Date : 2016-08-25 DOI: 10.4172/2157-7552.1000176

W. Mbinda, S. Anami, O. Ombori, C. Dixelius, Richard O. Oduor

{"title":"Efficient Plant Regeneration of Selected Kenyan Sweetpotato (Ipomoea batatas (L.) Lam.) Cultivars through Somatic Embryogenesis","authors":"W. Mbinda, S. Anami, O. Ombori, C. Dixelius, Richard O. Oduor","doi":"10.4172/2157-7552.1000176","DOIUrl":"https://doi.org/10.4172/2157-7552.1000176","url":null,"abstract":"Sweetpotato is an important food crop in the world as well as in Kenya. Various fungal, and viral diseases are major constraints in its production and are currently threatening the sweetpotato production in sub-Saharan Africa. Genetic engineering offers significant potential for the crop’s genetic improvement. However, this is limited by the low efi¬Âciency and strong genotype dependency in tissue culture. This study aimed to establish an efficient somatic embryogenesis and plant regeneration system using shoot apical meristem explants of sweetpotato. Three sweetpotato cultivars that are widely grown in Kenya; KSP36, Kemb36 and Mweu mutheke along with an exotic model cultivar Jewel were evaluated. The maximum somatic embryogenic induction, at 96.72%, was obtained from explants cultured on Linsmaier and Skoog salts and vitamins medium supplemented with 0.5 mg/l dichlorophenoxyacetic acid and 0.2 mg/l zeatin riboside. The highest number of shoot induction (33) was observed after transfer of embryonic callus to embryo maturation medium supplemented with 2 mg/l abscisic acid. Significant differences were observed between cultivars for somatic embryogenesis and plant regeneration. Jewel showed the best response, while Mweu mutheke was the least responsive under the culture conditions tested in this study. Regenerated plants were successfully rooted and grown to maturity after hardening in soil in the greenhouse. Such a robust, successful and efi¬Âcient system possesses the potential to become an important tool for crop improvement and functional studies of genes in sweetpotato.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":"214 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2016-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72655795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8