Journal of the Preventive Veterinary Medicine最新文献_第10页

Comparison of novel recombinant nucleoprotein ELISA with commercial ELISA for Newcastle disease 新型重组核蛋白酶联免疫吸附试验与市售酶联免疫吸附试验检测新城疫的比较

Journal of the Preventive Veterinary Medicine Pub Date : 2021-03-31 DOI: 10.13041/JPVM.2021.45.1.53

Jihoon Ryu, Hyeongseok Yoon, H. Moon, Hyun-Jin Shin

引用次数: 0

Detection of Mycobacterium bovis in environmental samples using nested PCR 巢式PCR检测环境样品中的牛分枝杆菌

Journal of the Preventive Veterinary Medicine Pub Date : 2021-03-31 DOI: 10.13041/JPVM.2021.45.1.30

Tae-woon Kim, Yunho Jang, M. K. Jeong, Jae-Myung Kim, Y. J. Lee, Seung-Bum Kim, Soon-Seek Yoon, Jeong-Soo Choi

{"title":"Detection of Mycobacterium bovis in environmental samples using nested PCR","authors":"Tae-woon Kim, Yunho Jang, M. K. Jeong, Jae-Myung Kim, Y. J. Lee, Seung-Bum Kim, Soon-Seek Yoon, Jeong-Soo Choi","doi":"10.13041/JPVM.2021.45.1.30","DOIUrl":"https://doi.org/10.13041/JPVM.2021.45.1.30","url":null,"abstract":"The detection of Mycobacterium bovis (M. bovis) in environmental samples with precision is imperative to control bovine tuberculosis (bTB) infections at the herd level, as residual M. bovis remains one of the major causes of recurring infections. In this study, a nested PCR method for the detection of M. bovis in environmental samples was applied to identify potential environmental reservoirs of the bacterium. A set of 200 environmental samples (167 fecal samples and 33 water samples) from 39 herds with a history of bTB outbreak was analyzed using a nested PCR method to detect residual M. bovis. Amplicon libraries of the IS6110 target gene fragment were amplified from M. bovis DNA using two established primer sets. A positive nested PCR result was observed in 69.5% of fecal samples and 66.7% of water samples, thus showing that residual M. bovis was present in the environmental samples of bTB-positive herds in a high proportion. This study is the first to demonstrate high levels of M. bovis DNA in environmental samples and to show that environmental reservoirs of this pathogen contribute to recurring outbreaks of bTB. Environmental monitoring of herds in which bTB outbreaks have occurred with high sensitivity and specificity is expected to help prevent the recurrence of potential bTB disease and improve the herd environment.","PeriodicalId":17310,"journal":{"name":"Journal of the Preventive Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82943786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigational study on the population of feral cats in Pyeongtaek city, 2019 2019年平泽市野猫种群调查研究

Journal of the Preventive Veterinary Medicine Pub Date : 2021-03-31 DOI: 10.13041/JPVM.2021.45.1.23

Ji-yeon Kim, Young Ah Lee, Chaeheon Chun, H. Lee, E. Jo, B. Hwang, Moon-Seok Yoon, E. Bae, Jeong-Hwan Yoon, Y. Jho, Soo Jung Lee, Jin Soo Han

{"title":"Investigational study on the population of feral cats in Pyeongtaek city, 2019","authors":"Ji-yeon Kim, Young Ah Lee, Chaeheon Chun, H. Lee, E. Jo, B. Hwang, Moon-Seok Yoon, E. Bae, Jeong-Hwan Yoon, Y. Jho, Soo Jung Lee, Jin Soo Han","doi":"10.13041/JPVM.2021.45.1.23","DOIUrl":"https://doi.org/10.13041/JPVM.2021.45.1.23","url":null,"abstract":"Feral cats are widely considered to be leading the potential impacts on public health. This study aimed to provide estimates of vital data for feral cats relating Trap-Neuter-Return (TNR) to establish strategies effectively to manage feral cats in Pyeongtaek. Thus, this study focused on estimating feral cat population in Pyeongtaek and conducted a comparative analysis of the data for feral cats in Seoul (2013). The number of feral cats was estimated from 23,069 to 26,655 in Pyeongtaek, 2019. In relation to human population, when comparing the number of feral cats of Pyeongtaek and Seoul, it ranged from 4.57% to 5.28%, and from 1.97% to 2.55% respectively. This showed that Pyeongtaek was higher than Seoul. Fewer kittens were found in high-density areas, which the TNR project is believed to be generally effective in controlling the number of feral cats. In conclusion, in urban and rural complexes such as Pyeongtaek City, the number of feral cats compared to the population was higher than that of Seoul City, and the TNR program is believed to be somewhat effective in controlling the number of feral cats. When implementing TNR, it is necessary periodically to investigate the population and reflect them in policymaking.","PeriodicalId":17310,"journal":{"name":"Journal of the Preventive Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74353468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in intramuscular hemoglobin concentration in a rabbit model of muscle atrophy using near-infrared diffuse optical spectroscopy 用近红外漫射光谱学研究兔肌肉萎缩模型肌内血红蛋白浓度的变化

Journal of the Preventive Veterinary Medicine Pub Date : 2021-03-31 DOI: 10.13041/JPVM.2021.45.1.1

Yikeun Kim, Chang Su Kim, Hu-Jang Lee, Yeh-Chan Ahn, Eun‐Kee Park

{"title":"Changes in intramuscular hemoglobin concentration in a rabbit model of muscle atrophy using near-infrared diffuse optical spectroscopy","authors":"Yikeun Kim, Chang Su Kim, Hu-Jang Lee, Yeh-Chan Ahn, Eun‐Kee Park","doi":"10.13041/JPVM.2021.45.1.1","DOIUrl":"https://doi.org/10.13041/JPVM.2021.45.1.1","url":null,"abstract":"A progressive muscle atrophy is strongly associated with aging, resulting in lower quality of life in elderly individuals. This study was conducted to determine relative hemoglobin (Hb) concentrations in the rabbit model of the sciatic nerve transection injury using non-invasive diffuse optical spectroscopy (DOS). From the 2nd week to the end of the experiment after sciatic nerve injury, a total muscle mass in nerve injured-group (NI group) significantly reduced compared with that in the normal group (p<0.001). During the capillary occlusion after nerve injury, the deoxy-hemoglobin (Hb-R) concentration in NI group significantly increased compared to that in the normal group at the 2nd and 3rd week after sciatic nerve injury (p<0.05). During the capillary release after nerve injury, the oxy-hemoglobin (Hb-O2) concentration in NI group significantly decreased at the 1st and 3rd week, and Hb-R significantly increased at 2nd week, compared to those in the normal group (p<0.05). Histological changes in the gastrocnemius muscle of NI group observed that clear fat filled spaces at the periphery of muscle fibers and angular fibers. From the results of this study, non-invasive DOS could be used to measure changes of Hb concentrations in muscles.","PeriodicalId":17310,"journal":{"name":"Journal of the Preventive Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89066192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-cancer effects of Fenbendazole and Paclitaxel combination on HL-60 cells 芬苯达唑联合紫杉醇对HL-60细胞的抗癌作用

Journal of the Preventive Veterinary Medicine Pub Date : 2021-03-31 DOI: 10.13041/JPVM.2021.45.1.13

Jihye Sung, H. Joo

{"title":"Anti-cancer effects of Fenbendazole and Paclitaxel combination on HL-60 cells","authors":"Jihye Sung, H. Joo","doi":"10.13041/JPVM.2021.45.1.13","DOIUrl":"https://doi.org/10.13041/JPVM.2021.45.1.13","url":null,"abstract":"Fenbendazole (FBZ) is one of the safest anthelmintic drugs. FBZ has been found to have anti-cancer effects by destabilizing microtubules. In this study, a synergistic effect of paclitaxel (PA), a microtubule-stabilizing anti-cancer agent, and FBZ was investigated on HL-60 cells, a human leukemia cell line. The metabolic activity of cells significantly decreased and the nucleus morphology upon the treatment of FBZ and PA based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay and Hoechst 33342 staining, respectively. To investigate the involvement of reactive oxygen species (ROS), the metabolic activity of the cells after treatment of N-acetyl-L-cysteine (NAC) was measured. Indeed, NAC significantly increased the metabolic activity of the cells treated with FBZ and PA, suggesting that both drugs affect at least in part via ROS. Furthermore, FBZ and PA increased cell death in an annexin V- fluorescein isothiocyanate/propidium iodide staining assay. Taken together, FBZ and PA have a synergistic anti-cancer activity on HL-60 cells at a certain concentration. These results may provide researchers and clinicians in cancer-related fields with some valuable information to broaden the use of FBZ.","PeriodicalId":17310,"journal":{"name":"Journal of the Preventive Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84666864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparative assessment of the biological toxicity of scyphozoan jellyfish species (Nemopilema nomurai, Cyanea nozakii, Aurellia aurita and Rhopilema esculentum) venoms 热带水母(Nemopilema nomurai, Cyanea nozakii, Aurellia aurita和Rhopilema esculentum)毒液的生物毒性比较评价

Journal of the Preventive Veterinary Medicine Pub Date : 2020-12-31 DOI: 10.13041/JPVM.2020.44.4.179

Hyeryeon Yang, Y. Lee, Jongwoon Seong, S. Choi, D. Hwang, Euikyung Kim, Changkeun Kang

引用次数: 0

Development of an analytical method for the determination of tulathromycin in porcine lung tissue using liquid chromatography-tandem mass spectrometry 建立液相色谱-串联质谱法测定猪肺组织中土拉霉素的分析方法

Journal of the Preventive Veterinary Medicine Pub Date : 2020-12-31 DOI: 10.13041/JPVM.2020.44.4.174

Jun-Ho Song, Kim Suk, Lee Hujang

引用次数: 0

Effects of canine mesenchymal stem cells-derived exosomes in a mouse model of canine mammary tumor 犬间充质干细胞来源的外泌体在犬乳腺肿瘤小鼠模型中的作用

Journal of the Preventive Veterinary Medicine Pub Date : 2020-12-31 DOI: 10.13041/JPVM.2020.44.4.147

So Yeon Jeong, Se-A Lee, N. Gu, Jienny Lee, Yoon-Hee Lee, B. Hyun

引用次数: 0

Spatial risk factors of the 2016 to 2018 highly pathogenic avian influenza epidemics in the Republic of Korea 2016 - 2018年韩国高致病性禽流感流行的空间风险因素分析

Journal of the Preventive Veterinary Medicine Pub Date : 2020-12-31 DOI: 10.13041/JPVM.2020.44.4.186

Eu-Tteum Kim, S. Pak

{"title":"Spatial risk factors of the 2016 to 2018 highly pathogenic avian influenza epidemics in the Republic of Korea","authors":"Eu-Tteum Kim, S. Pak","doi":"10.13041/JPVM.2020.44.4.186","DOIUrl":"https://doi.org/10.13041/JPVM.2020.44.4.186","url":null,"abstract":"The current study explored the epidemiological associations between the 2016/18 highly pathogenic avian influenza (HPAI) epidemics and spatial factors, including the distance from a poultry farm to the closest groundwater source, migratory bird habitat, eco-natural area, and poultry farm altitude. We included 14 spatial factors as independent variables. The variables were used in the original continuous measurement format. In total, 288 poultry farms (144 HPAI-confirmed and 144 non-confirmed) were used as the dependent variable. In addition, the variables’ continuous measurement was converted to a categorical measurement format by using a general additive model. For risk factor analysis based on the continuous measurements of spatial factors, the non-graded eco-natural area distance (odds ratio [OR]: 1.00) and the grade one eco-natural area distance (OR: 0.99) were statistically significant independent variables. However, in the risk factor analysis based on the categorical measurement format of the spatial factors, the non-graded eco-natural area distance (OR: 0.08) and poultry farm altitude (OR: 0.44) were statistically significant independent variables in both a univariate and multiple logistic regression model. In other words, when a poultry farm was located far from the non-graded eco-natural area or in a highland area, the likelihood of an HPAI epidemic would decrease. From an HPAI control perspective, it is recommended that the government apply increased levels of biosecurity measures, such as bird-nets, fences, intensive disinfection of equipment, and regular bird health monitoring, for poultry farms located near non-graded eco-natural areas or in a lowland area.","PeriodicalId":17310,"journal":{"name":"Journal of the Preventive Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82859686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin sensitization test of potassium hydroxide in guinea pigs 氢氧化钾豚鼠皮肤致敏试验

Journal of the Preventive Veterinary Medicine Pub Date : 2020-12-31 DOI: 10.13041/JPVM.2020.44.4.169

D. Hwang, Suk Kim, Euikyung Kim, Hu-Jang Lee

{"title":"Skin sensitization test of potassium hydroxide in guinea pigs","authors":"D. Hwang, Suk Kim, Euikyung Kim, Hu-Jang Lee","doi":"10.13041/JPVM.2020.44.4.169","DOIUrl":"https://doi.org/10.13041/JPVM.2020.44.4.169","url":null,"abstract":"This study was to evaluate the skin sensitization of potassium hydroxide (KOH) by the guinea pig maximization test. In the preliminary test, the induction and challenge dose of KOH were determined as 1.0 and 0.2%, respectively. Guinea pigs were sensitized with intradermal injection of 0.1ml KOH for 24 h. After 1 week, 1.0% of KOH was treated on the site of injection and 0.2% of KOH was challenged 2 weeks later. The average skin reaction evaluated by erythema and edema on the challenge sites and sensitization rate in the KOH-treatment group at 24 h were 0.0 and 0%, respectively, which are substantially low compared with in the 2,4-dinitrochlorobenzene-treatment group (positive control group) (average skin reaction: 3.0, sensitization rate: 100%) and identical with in the distilled water-treatment group (negative control group), representing a weak sensitizing potential. The average skin reaction and sensitization rate observed at 48 h were 0.0 and 0% in the KOH-treatment group, respectively, and 2.0 and 100% in the positive control group, respectively. It was concluded that 1.0% of KOH classified to Grade I, induced no sensitization when tested in guinea pigs.","PeriodicalId":17310,"journal":{"name":"Journal of the Preventive Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85911410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0