Journal of Stem Cell Research & Therapeutics最新文献

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Therapeutic role of mesenchymal stem cells seeded dermal matrix versus acellular dermal matrix in healing of skin defect 间充质干细胞种子真皮基质与脱细胞真皮基质在皮肤缺损愈合中的治疗作用

Journal of Stem Cell Research & Therapeutics Pub Date : 2019-02-25 DOI: 10.15406/jsrt.2019.05.00128

G. Mohamed, M. Moussa, Sahar, M. W. Omar, A. A. Zeid, W. Baher, A. Mohammed, M. Sobhy, Ahmed, Sabry, O. Adel, M. Salem, Omar

{"title":"Therapeutic role of mesenchymal stem cells seeded dermal matrix versus acellular dermal matrix in healing of skin defect","authors":"G. Mohamed, M. Moussa, Sahar, M. W. Omar, A. A. Zeid, W. Baher, A. Mohammed, M. Sobhy, Ahmed, Sabry, O. Adel, M. Salem, Omar","doi":"10.15406/jsrt.2019.05.00128","DOIUrl":"https://doi.org/10.15406/jsrt.2019.05.00128","url":null,"abstract":"Background & objectives: One of the major challenges facing the surgeons is replacing a full-thickness skin loss successfully. This study aimed at testing the efficacy of decellularized dermal matrix seeded with bone marrow-mesenchymal stem cells (BM-MSCs) as a scaffold for the repair of skin defects in rats comparison to using acellular dermal matrix (ADM) alone. Methods: A 2×2 cm2 size full thickness skin defect was created on the dorsum of thirty male Wister rats (200- 250g) under xylazine (5 mg/kg) and ketamine (50 mg/kg) anesthesia. The animals were then randomly divided into three equal groups: group I; The defect was left for spontaneous recovery, group II; The defect was repaired with ADM alone, and group III;Tthe defect was repaired with ADM seeded with labeled BM-MSCs. The healing rate of the defect in all groups was assessed by measuring wound area and healing percentage twice weekly. The specimens from the wound site were obtained from all groups on day 14 and day 28 post-operative for histological analysis. Results: Treatment of wound defect with BM-MSCs seeded dermal matrix resulted incomplete wound recovery on gross examination. Moreover, histological analysis showed proper reepithelization, proper collagen rearrangement together with minimal inflammatory cells. Well developed hair follicles and sebaceous glands were noted as well. Statistically, 28 days post-operatively, significant increase in healing rate, healing area percentage and collagen area percentage was detected together with significant decrease in vascular density compared to group I&II. Conclusion: Stem cells seeded ADM facilitated early and better healing of skin defect in rats than the non- seeded ADM and spontaneous healing.","PeriodicalId":172569,"journal":{"name":"Journal of Stem Cell Research & Therapeutics","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126345712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Behavior of adipocytes in the mammary niche during pregnancy and lactation 妊娠和哺乳期乳腺生态位脂肪细胞的行为

Journal of Stem Cell Research & Therapeutics Pub Date : 2019-02-02 DOI: 10.15406/jsrt.2019.05.00126

Érika C de Alvarenga, Rebecca Vasconcellos, Caroline C. Picoli, Raquel Alves Cost, Nathanael Vieira Medrado, Raisa Mileib, Rosiane Castro, Vicencia M Sales

{"title":"Behavior of adipocytes in the mammary niche during pregnancy and lactation","authors":"Érika C de Alvarenga, Rebecca Vasconcellos, Caroline C. Picoli, Raquel Alves Cost, Nathanael Vieira Medrado, Raisa Mileib, Rosiane Castro, Vicencia M Sales","doi":"10.15406/jsrt.2019.05.00126","DOIUrl":"https://doi.org/10.15406/jsrt.2019.05.00126","url":null,"abstract":"Primarily, the adipose tissue is known for its function storing excess energy as fat. This tissue is also an endocrine organ and communicates with several tissues, such as muscle, liver and brain. In addition, the adipose tissue is an accessible source of adult stem cells. Autologous adipose tissue transplant used for plastic surgery purposes take advantage of this feature. These pluripotent cells are also targets for gene therapy and autologous transplant in regenerative medicine. A recent study using state-of-art technology explores the plasticity within mature adipocyte into stroma of the mammary gland. Strikingly, adipocyte-derived preadipocytes de-differentiate and re-differentiate repeatedly, during pregnancy to accommodate the new imposed body metabolic demand. The emerging knowledge from this study is essential to understand the cellular processes occurring into the mammary gland during pregnancy, lactation, and involution. This idea opens new perspectives to study the involvement of the adipose tissue in systemic regulatory processes during these transitional states and has implication on conditions as cancer and inflammation-related diseases.","PeriodicalId":172569,"journal":{"name":"Journal of Stem Cell Research & Therapeutics","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126612551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

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Journal of Stem Cell Research &amp; Therapeutics最新文献

Journal of Stem Cell Research & Therapeutics最新文献