Journal of receptor research最新文献_第3页

Arginine vasopressin receptor internalization and recycling in rat renal collecting tubules. 精氨酸加压素受体在大鼠肾收集小管中的内化和循环。

Journal of receptor research Pub Date : 1994-02-01 DOI: 10.3109/10799899409067001

J K Kim, S N Summer, R W Schrier

{"title":"Arginine vasopressin receptor internalization and recycling in rat renal collecting tubules.","authors":"J K Kim, S N Summer, R W Schrier","doi":"10.3109/10799899409067001","DOIUrl":"https://doi.org/10.3109/10799899409067001","url":null,"abstract":"Arginine vasopressin (AVP) binds to two distinct receptors to initiate vasopressor (V1 receptor) and hydroosmotic actions (V2 receptor). Internalization and recycling of the V1 receptor in cultured vascular smooth muscle cells and hepatocytes have recently been demonstrated. However, the receptor cycle of the AVP V2 receptor in the renal collecting tubules has not yet been well defined. Therefore, the present study was undertaken to investigate the AVP V2 receptor cycle, including AVP binding to the surface receptor, internalization and potential recycling in isolated outer medullary collecting tubules. The maximal AVP surface binding was reached in 10 min, and 25 micrograms/ml trypsin completely inhibited the surface binding. A Scatchard plot of 125I-AVP surface binding indicated a single population of V2 receptors with a Kd of 1.92 x 10(-9) M and a Bmax of 1.77 x 10(-11) M or 590 fmoles/mg protein. 81.7% (72-85%) of specific bound receptor was internalized (specific surface binding: 742.8 +/- 111.1 vs internalized binding: 607.3 +/- 27.8 fmoles bound/mg protein). More than 90% of surface bound receptor was recycled to the cell surface after internalization (control surface binding: 584.0 +/- 64.0 vs recycled surface binding: 546.6 +/- 32.0 fmoles bound/mg protein). Cycloheximide (40 micrograms/ml) did not inhibit the receptor recycling (control recycled surface binding: 546.6 +/- 32.0 vs cycloheximide recycled surface binding: 505.0 +/- 54.8 fmoles bound/mg protein), thus suggesting that the receptors were not resynthesized after dissociation from the receptor-ligand complex. These studies therefore demonstrate that the AVP V2 receptor is internalized and recycled in the rat renal collecting tubule.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"14 2","pages":"139-52"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899409067001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19172757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Identification of alpha 1-adrenoceptor subtypes in rat lung by binding of [3H]-prazosin and [3H]-WB4101. [3H]-prazosin和[3H]-WB4101结合鉴定大鼠肺α 1-肾上腺素受体亚型。

Journal of receptor research Pub Date : 1994-02-01 DOI: 10.3109/10799899409066998

Y Hiramatsu, R Muraoka, S Kigoshi, I Muramatsu

{"title":"Identification of alpha 1-adrenoceptor subtypes in rat lung by binding of [3H]-prazosin and [3H]-WB4101.","authors":"Y Hiramatsu, R Muraoka, S Kigoshi, I Muramatsu","doi":"10.3109/10799899409066998","DOIUrl":"https://doi.org/10.3109/10799899409066998","url":null,"abstract":"The alpha 1-adrenoceptor subtypes in rat lung were characterized according to their binding of [3H]-prazosin or [3H]-WB4101 and were compared with that in rat liver. [3H]-prazosin bound with high affinity to an apparently homogeneous population of sites in rat lung. The binding of [3H]-prazosin was inhibited by WB4101, benoxathian and 5-methylurapidil biphasically but the proportions differed between WB4101 or benoxathian and 5-methylurapidil. In the lung membranes pretreated with chloroethylclonidine a single population with high affinity for WB4101 and benoxathian was detected while 5-methylurapidil still discriminated two sites of distinctly different affinities. These results suggest that the WB4101-high affinity sites of rat lung were subdivided further into two subclasses according to 5-methylurapidil binding affinity. In fact, [3H]-WB4101 bound to lung membranes with two different affinities and the high affinity binding sites were subdivided by 5-methylurapidil into two classes. By contrast, [3H]-prazosin or [3H]-WB4101 binding sites of liver membranes were detected as a single population with high affinity for prazosin but with low affinity for WB4101, benoxathian and 5-methylurapidil. These results suggest that the alpha 1-adrenoceptors of rat lung are composed of three distinct subtypes (alpha 1A, alpha 1B and unknown subtypes) while that of liver is of alpha 1B subtype. Two radioligands with different affinities may be used as powerful probes to identify receptor subclasses.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"14 2","pages":"75-98"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899409066998","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18906675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Two alpha 1b-adrenergic receptor mRNAS expressed in Sprague-Dawley rat liver have distinct 5'-regions. 在Sprague-Dawley大鼠肝脏中表达的两个α - 1b肾上腺素能受体mrna具有不同的5′-区。

Journal of receptor research Pub Date : 1994-02-01 DOI: 10.3109/10799899409067000

C L Deng, L E Cornett

引用次数: 7

Characterization of the membranous antiestrogen binding protein: I. Partial purification of the protein in its active state. 膜性抗雌激素结合蛋白的表征:1 .活性蛋白的部分纯化。

Journal of receptor research Pub Date : 1994-01-01 DOI: 10.3109/10799899409066994

C Chailleux, M Poirot, F Mesange, F Bayard, J C Faye

引用次数: 7

Characterization of the membranous antiestrogen binding protein: II. Purification to homogeneity. 膜性抗雌激素结合蛋白的研究:2。净化到同质性。

Journal of receptor research Pub Date : 1994-01-01 DOI: 10.3109/10799899409066995

M Poirot, C Chailleux, F Mesange, F Bayard, J C Faye

引用次数: 8

Labelling of D2-dopaminergic and 5-HT2-serotonergic binding sites in human trophoblastic cells using [3H]-spiperone. 用[3H]-spiperone标记人滋养细胞中d2 -多巴胺能和5- ht2 - 5-羟色胺能结合位点。

Journal of receptor research Pub Date : 1994-01-01 DOI: 10.3109/10799899409066993

C Vaillancourt, A Petit, N Gallo-Payet, D Bellabarba, J G Lehoux, S Bélisle

{"title":"Labelling of D2-dopaminergic and 5-HT2-serotonergic binding sites in human trophoblastic cells using [3H]-spiperone.","authors":"C Vaillancourt, A Petit, N Gallo-Payet, D Bellabarba, J G Lehoux, S Bélisle","doi":"10.3109/10799899409066993","DOIUrl":"https://doi.org/10.3109/10799899409066993","url":null,"abstract":"We previously reported that dopamine (DA) inhibited the release of human placental lactogen (hPL) from human placental cells. We also demonstrated the presence of D2-dopamine receptors in membrane preparations of human term placenta. The aim of the present study was to characterize these D2 receptors on freshly isolated human trophoblastic cells. The binding of [3H]-spiperone to these cells showed a curvilinear Scatchard plot suggesting the presence of two classes of binding sites (Kd1 = 1.26nM; Kd2 = 44.3nM). Competition experiments showed the following inhibitory binding potencies: serotonin-2 (5-HT2) > or = D2 >>> alpha-adrenergic, beta-adrenergic, D1-dopamine, thus suggesting the presence of 5-HT2 binding sites. We have examined this possibility by blocking [3H]-spiperone binding to 5-HT2 receptors in the presence of 50nM ketanserin, a selective antagonist of 5-HT2 sites. Under this condition, the linear Scatchard plot obtained suggested a single population of homogeneous binding sites for [3H]-spiperone with a Kd of 0.55nM. To further characterize placental D2 receptors we conducted binding experiments with [3H]-raclopride, an more selective D2 antagonist. The linear Scatchard plot obtained with this ligand suggested one class of binding sites for [3H]-raclopride (Kd = 6nM) with the following inhibitory potencies: D2 >>> beta-adrenergic >> 5-HT2, D1, alpha-adrenergic. These results suggest an important paracrine function for DA in human placenta and show for the first time that [3H]-spiperone binds putative 5-HT2 receptors in human placenta.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"14 1","pages":"11-22"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899409066993","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19149426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Induction of beta 2-adrenergic receptor mRNA and ligand binding in HeLa cells. β 2-肾上腺素能受体mRNA与配体结合在HeLa细胞中的诱导作用。

Journal of receptor research Pub Date : 1994-01-01 DOI: 10.3109/10799899409066992

R S Duman, P H Fishman, J F Tallman

引用次数: 5

Internalization of N-formyl peptide chemotactic receptor-ligand complex by human neutrophils. The role of the receptor's 2-kDa external domain and carbohydrates. 中性粒细胞内化n -甲酰基肽趋化受体-配体复合物。受体的2-kDa外部结构域和碳水化合物的作用。

Journal of receptor research Pub Date : 1994-01-01 DOI: 10.3109/10799899409066996

J Remes, U Petäjä-Repo, H Rajaniemi

{"title":"Internalization of N-formyl peptide chemotactic receptor-ligand complex by human neutrophils. The role of the receptor's 2-kDa external domain and carbohydrates.","authors":"J Remes, U Petäjä-Repo, H Rajaniemi","doi":"10.3109/10799899409066996","DOIUrl":"https://doi.org/10.3109/10799899409066996","url":null,"abstract":"We treated human neutrophils with papain to remove the external 2-kDa domain and along with it the two oligosaccharide side chains of the N-formyl peptide chemotactic receptor and investigated what effect their absence has on the ligand-receptor complex internalization. After prelabeling of the cells with 125I-hexapeptide for 5 min at 22 degrees C, about 95% of the bound radioactivity was located on the cell surface. During the first 5-min incubation at 37 degrees C both the control and papain-treated cells internalized 73% of the receptor-ligand complexes suggesting that internalization is very rapid in human neutrophils and that removal of the external domain and the carbohydrates of the receptor does not affect the rate. However, the truncated receptor-ligand complexes were degraded at a faster rate because the radioactivity released into the medium was significantly higher and correspondingly the acid-resistant radioactivity significantly lower in the papain-treated neutrophils than in control cells already at 5 min and all subsequent time points. The radioactivity accumulated in the medium of the control and papain-treated neutrophils represented inactivated 125I-hexapeptide as less than 5% of it at 5, 30 and 120 min were capable of rebinding. No receptor recycling was detected in either cells. These results indicate that removal of the 2-kDa external domain and the carbohydrates of the N-formyl chemotactic receptors has little effect on the internalization rate of the receptor-ligand complexes but accelerates markedly their intracellular degradation.","PeriodicalId":16948,"journal":{"name":"Journal of receptor research","volume":"14 1","pages":"47-62"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10799899409066996","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19149429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and solubilization of a recombinant human neurokinin-1 receptor in insect cells. 重组人神经激肽-1受体在昆虫细胞中的表达与溶解。

Journal of receptor research Pub Date : 1994-01-01 DOI: 10.3109/10799899409066997

K E Mazina, C D Strader, T M Fong

引用次数: 18

Heterotrimeric G proteins. Molecular diversity and functional correlates. 异三聚体G蛋白。分子多样性和功能相关性。

Journal of receptor research Pub Date : 1993-01-01 DOI: 10.3109/10799899309073642

L Birnbaumer

引用次数: 8