{"title":"Antibiotic Resistance in Lactic Acid Bacteria","authors":"Deyan Tong","doi":"10.35248/2329-8901.21.9.234","DOIUrl":"https://doi.org/10.35248/2329-8901.21.9.234","url":null,"abstract":"Most starter cultures belong to the lactic acid bacteria recognized as safe by the US Food and Drug Administration (FDA) and the European Food safety Authority. However, LAB may act as intrinsic reservoirs for antibiotic resistance (AR) genes. This fact may not constitute a safety concern itself, as the resistance gene transfer is vertical. Nevertheless, external genetic elements may induce changes that favour the horizontal transfer transmission of resistance from pathogens as well as from the human intestinal microbiota, which represents a severe safety issue. Some genus of AR LAB includes Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, and Streptococcus isolated from fermented meat and milk products. Currently, the WHO recommends that LAB used in the food industry should be free of resistance.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"194 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79799068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunologically Tolerated Despite of Human Pancreas","authors":"S. Bouzari","doi":"10.35248/2329-8901.21.9.E128","DOIUrl":"https://doi.org/10.35248/2329-8901.21.9.E128","url":null,"abstract":"","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"23 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81437650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Probiotics and Health in the year 2021","authors":"S. Patole","doi":"10.35248/2329-8901.21.9.E127","DOIUrl":"https://doi.org/10.35248/2329-8901.21.9.E127","url":null,"abstract":"","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"11 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91028037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Industry Originated Probiotic Bacteria Role in COVID-19","authors":"Àlex, E.A. Marakhovsky","doi":"10.35248/2329-8901.21.9.227","DOIUrl":"https://doi.org/10.35248/2329-8901.21.9.227","url":null,"abstract":"Intensive, uncontrolled, long term usage of industry originated Bifidobacterium animalis subspecies lactis by humans lead the “Probiotic contamination” of human gut, when unusual, not common for healthy individuals bacterial colonies have being created and dominate for a long time, changing the function of Human Microbiome. Several strains of Bifidobacterium animalis subspecies lactis (produced by few leading world biotechnological manufacturers) colonize human mucosa surfaces, lead the SARS-CoV-2 increased ability to penetrate Air path and gastrointestinal mucosa surfaces, change an innate immunity of individuals, infect human cells and ensure specific complications, as cytokine storm, atypical pneumonia, clotting. SARS-CoV-2 features (as interaction with ACE2 protein, others possible cell-entry points, binding to human cell surface and endocytosis) are enhanced by above bacterial metabolites. Further on, Bifidobacterium animalis subspecies lactis or metabolically similar one, provide deregulated release of pro and anti-inflammatory cytokines, lead a severe course of COVID-19.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"9 11 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76059460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Van Vlasselaer Martin, Dillemans Monique, Vandel Laurence
{"title":"Disruption Potential of a Multi-species Oral Biofilm by Probiotics: PreliminaryInvestigation of a New Bacillus subtilis Strain","authors":"Van Vlasselaer Martin, Dillemans Monique, Vandel Laurence","doi":"10.35248/2329-8901.21.9.233","DOIUrl":"https://doi.org/10.35248/2329-8901.21.9.233","url":null,"abstract":"Probiotics may represent an appropriate alternative to oral therapy by specifically targeting pathogens during the successive phases of biofilm “formation”. The aim of this work was to find ways to complement and strengthen this anti-biofilm action through examining the effects of 13 probiotics on the “preformed” three-dimensional biofilm structure. An in vitro dental multi-species biofilm model has been developed using pioneer-colonizing streptococci strains Streptococcus mutans, Streptococcus sobrinus, and Streptococcus oralis subsp. oralis with the addition of Actinomyces naeslundii, to obtain highly heterogeneous strong biofilm architecture. Among the different probiotic genera tested, only the genus Bacillus had a significant disruptive impact on biofilm. B. subtilis NOH (Natto Original Habitat) isolated from a traditional Japanese fermented food (natto) was the most effective disrupter, able to destroy 39% of pre-formed biofilm. Some results have suggested the presence of biosurfactants in its supernatant. Unlike B. subtilis NOH, the B. subtilis CU1 (CNCM I-2745) strain showed strong antibacterial properties against pathogenic organisms and blocked biofilm development at high probiotic concentration. A dual strategy of applying agents with disruptive (B. subtilis NOH) and antimicrobial (B. subtilis CU1) activity could be an interesting approach, with disassembling of the matrix structure exposing pathogenic organisms to more efficient killing.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"16 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87940322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Fantinato, H. R. Carvalho, Ana Lucia Orl, inni Pilleggi de Souza
{"title":"Yogurt for the Equilibrium of the Oral Microbiota with the New Streptococcussalivarius Probiotic BIO5","authors":"V. Fantinato, H. R. Carvalho, Ana Lucia Orl, inni Pilleggi de Souza","doi":"10.35248/2329-8901.21.9.232","DOIUrl":"https://doi.org/10.35248/2329-8901.21.9.232","url":null,"abstract":"This study was carried out to assess whether the use of yogurt prepared with the strain of the probiotic Streptococcus salivarius BIO5, could interfere in any way in the oral microbiota of students. Sixty students aged between 4 and 14 years were selected, who took the yogurt three times a week, for a period of 90 days. Student’s saliva was collected to check for presence and quantify the following microorganisms: staphylococci, total streptococci, mutans streptococci, lactobacilli, pseudomonas, yeasts, anaerobes and enterobacteria. The results showed that after 90 days of using yogurt, there was a decrease in microorganisms that are not part of the oral microbiota and there was no change in microorganisms considered to be residents.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"413 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84886558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Deidda, Teresa Graziano, A. Amoruso, A. Prisco, M. Pane, M. D. Piano, L. Mogna
{"title":"How Probiotics may Kill Harmful Bacteria: The in vitro Activity against Some Haemolytic Strains","authors":"F. Deidda, Teresa Graziano, A. Amoruso, A. Prisco, M. Pane, M. D. Piano, L. Mogna","doi":"10.35248/2329-8901.20.8.216","DOIUrl":"https://doi.org/10.35248/2329-8901.20.8.216","url":null,"abstract":"Haemolysis is the lysis of red blood cells (RBCs) and the subsequent release of their contents into surrounding fluid. Several pathogens, including Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa are able to cause haemolysis in vitro and in vivo. A vast body of scientific literature has demonstrated the ability of certain probiotic bacteria to antagonize grampositive and gram-negative strains by secreting soluble molecules named bacteriocins. Anyway, insufficient data is currently available in relation to haemolytic bacteria. Ten lactobacilli were selected for this in vitro study. The agar spot assay was employed to quantify any possible inhibition. The diameters of inhibition zones around the spots were measured. Our results showed that selected probiotics could exert a focused protective effect against pathogenic bacteria responsible for RBCs lysis at various extent. Further investigations will be needed to study the underlying molecules responsible for inhibition.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"28 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78967951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SARS-CoV-2 and Microbiota","authors":"S. Schippa, F. Pantanella","doi":"10.4172/2329-8901.1000217","DOIUrl":"https://doi.org/10.4172/2329-8901.1000217","url":null,"abstract":"This letter exposes some of the many reasons why microbiotamodulation in patients with COVID-19 disease and more ingeneral, in all people at greatest risk of contracting SARS-CoV2virus infections, should be taken into consideration.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"83 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77647464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative Evaluation of Lactobacilli and Bifidobacteria in Healthy Individuals","authors":"O. Fadare, S. Sabri","doi":"10.35248/2329-8901.20.8:224","DOIUrl":"https://doi.org/10.35248/2329-8901.20.8:224","url":null,"abstract":"Lactobacillus and Bifidobacterium spp. are known as the major group that constitutes probiotic bacteria that form part of the normal biota of humans. The ecological significance of probiotic bacteria in the human gut cannot be exaggerated as studies have revealed many of the health benefits they offer. However, much is yet to be known about its population variation between and within individuals. Five apparently healthy volunteers were recruited and advised not to consume yogurt, antibiotics, alcohol and steroid three weeks before and during the study. Three (3) fecal samples from each participant obtained at two weeks interval (total of 15 samples a week) for six weeks were examined for each subject bacteria. Samples were collected in sterile specimen jars and immediately taken to the laboratory for analysis. Rogosa and BIM-25 selective media were used for the enumeration of Lactobacillus spp. and Bifidobacterium spp. respectively using plate count method. Lactobacilli were classified at the specie level using API 50 CHL kit, while Bifidobacteria isolates were identified at the genus level by detecting the presence of Fructose-6-phosphate phosphorketolase (F6PPK) activity. Both groups of bacteria were also identified at the genus level using a genus-specific primer set. All selected isolates obtained from the respective culture media were confirmed to be Lactobacilli and Bifidobacteria. The data obtained showed that intra-individual variation of the population of Lactobacillus and Bifidobacterium spp. was significantly lower than inter-individual variation at P<0.05. This study establishes the fact that the composition level of Lactobacillus and Bifidobacterium spp. varies between healthy individuals, but with little or no variation within healthy individuals.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"199 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86539123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuki Ikeda, M. Nasu, C. Klotz, Jean-Yves Bruxer, Diane Plaisant
{"title":"A Prospective, Randomized, Double Blind, Two Arms, Parallel, Placebo Controlled, Clinical Study to Evaluate the Efficacy of OPTIMEALTH FOOD P Supplementation in Modulating Gut Microbiome in Healthy Adults","authors":"Yuki Ikeda, M. Nasu, C. Klotz, Jean-Yves Bruxer, Diane Plaisant","doi":"10.35248/2329-8901.20.8:222","DOIUrl":"https://doi.org/10.35248/2329-8901.20.8:222","url":null,"abstract":"A structured and equilibrated gut microbiome is needed for optimal health status. Factors such as antibiotic use, caesarean-section deliveries, excessive hygiene, stress, and lack of exercise have a major impact on the microbiome which further leads to microbiota shift. This shift may trigger the development of several disorders, such as obesity, metabolic syndrome, cardiovascular diseases, colorectal cancer and Inflammatory Bowel Diseases (IBD). The present study was designed to evaluate the efficacy and safety of the formulation OPTIMEALTH® FOOD P (OF PB-LBF) on gut microbiota population. OPTIMEALTH® FOOD P® is composed of more than 300 metabolites produced by a selected complex of lactic acid bacteria strains through high tech Fermentation Technology. OPTIMEALTH® FOOD P was developed by studying the microbiota of healthy centenarians who have a particular microbiota profile. A total of 59 subjects compliant with inclusion criteria were randomly assigned to one of the two groups, OPTIMEALTH® FOOD P or placebo group using a simple randomization process. Consumption of OPTIMEALTH® FOOD P by healthy men and women with self-selected diets and unchanged physical activity patterns resulted in the group of OPTIMEALTH® FOOD P in an increased of Bifidobacterium longum by 27.5%, Lactobacillus acidophilus by 24.6%, Lactobacillus plantarum by 28.6%, Roseburia intestinalis by 14.5%, Faecalibacterium prausnitzii by 23.4% and an increase of Akkermansia muciniphila by 11.11%. In conclusion, supplementation with OPTIMEALTH® FOOD P resulted in significant gut microbiota modulation. The tolerance was good among subjects. No side effects or adverse events were reported during the study. Hence OPTIMEALTH® FOOD P at 100 mg/day could be recommended as a safe solution to modulate gut microbiota and improve diseases related to microbiota dysbiosis.","PeriodicalId":16865,"journal":{"name":"Journal of Probiotics & Health","volume":"261 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80130016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}