Chandra Mohini Nemkul, G. B. Bajracharya, Ila Shrestha
{"title":"Assessment of Antimicrobial and Antioxidant Activities of Four Ethnomedicinal Plants Used by Magars in Nawalpur District, Nepal","authors":"Chandra Mohini Nemkul, G. B. Bajracharya, Ila Shrestha","doi":"10.3126/bdpr.v20i2.57030","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.57030","url":null,"abstract":"Ethnobotanical survey in the Magar villages in Nawalpur district, Gandaki province, Nepal revealed that root of Phanera vahlii (Wight & Arn.) Benth, bark of Rhododendron arboreum Sm. and flower of Woodfordia fruticosa (L.) Kurz, have been in use for diarrhea and dysentery. Root of Thalictrum foliolosum DC. has been used for the treatment of rheumatic pain. The study aimed to evaluate antimicrobial and antioxidant effects as well as total phenol content in Phanera vahlii, Rhododendron arboreum, Woodfordia fruticosa and Thalictrum foliolosum. Total phenol content (TPC) was estimated using Folin-Ciocalteu method. 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) free radical and hydrogen peroxide scavenging assays were used to evaluate the antioxidant capacity. Antibacterial effect was established by the Agar well diffusion assay. The minimum TPC value of 65.78±3.44 mg GAE/g dry extract was estimated in 70% methanolic extract of Thalictrum foliolosum and maximum TPC value of 258.40±6.26 mg GAE/g dry extract was estimated in 70% methanolic extract of Woodfordia fruticosa. IC50 value range 21.59±0.26 µg/ml in Rhododendron arboreum to 1124.79±3.69 µg/ml in Thalictrum foliolosum was calculated in DPPH free radical scavenging assay. Scavenging of DPPH free radical range from 12.40% to 94% at 100 µg/mL concentration of 70% methanolic extracts were estimated and scavenging of hydrogen peroxide range from 36% to 73%. The maximum zone of inhibition (ZOI) against Escherichia coli at loading dose of 5 mg of the extract observed was 18±0.73 mm by 70% methanolic extract of Woodfordia fruticosa and its minimum inhibition concentration (MIC) was <1.56 µg /ml.The extracts efficiently inhibited the growth of Escherichia coli verifying the rural knowledge. At the same time, the extracts displayed efficient antioxidant activity.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90501976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Traditionally Important Herbaceous Medicinal Plants of Majathal Wildlife Sanctuary, District Solan, Himachal Pradesh, North Western Himalaya, India","authors":"K. Kumari, R. Verma","doi":"10.3126/bdpr.v20i2.57035","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.57035","url":null,"abstract":"The Himalayas is one of the mega diversity hotspots of the world, which contribute a great to the biodiversity wealth of the world. Among the biogeographic provinces of India, the Indian Himalayan Region (IHR) is well known for its diversity of ethnomedicinal plants. Majathal Wildlife Sanctuary is situated in District Solan, Himachal Pradesh, North Western Himalaya, India. In the present study, social surveys were conducted through the application of semi-structured questionnaire. All 39 villages inside and near the boundary (up to 2 km) were selected for the survey in the sanctuary. All the houses were surveyed and people of different ages and sexes were asked and their responses were recorded. A reconnaissance survey was conducted for the floristic diversity in the area and plant samples were collected for identification and herbarium preparation. In the sanctuary, there were 51 species of herbaceous medicinal plants identified which were used by local people. The results showed that the knowledge regarding medicinal plants was very less in the new generation as compared to the old age and middle age people. This study will help to know more medicinal plants from the villages and procure knowledge for future studies. This documented information will be preserved and utilized for mankind and make local communities economically stable and healthy.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89051564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anatomical Study of Shorea robusta Gaertn.","authors":"P. Chalise, Y. R. Paneru, L. Joshi","doi":"10.3126/bdpr.v20i01.56599","DOIUrl":"https://doi.org/10.3126/bdpr.v20i01.56599","url":null,"abstract":"Shorea robusta, commonly known as Sal, is one of the multipurpose timber trees in Nepal. This study aims to carry out detailed anatomical investigation of the wood and leaf anatomical traits of Sal. The wood and leaf samples of Sal were collected from Rupandehi district, Nepal. Wood of Sal was found to be diffuse-porous and the vessels were mostly solitary or paired and sometimes in a short radial multiple. Non-septate, vasicentric fibres forming solid tracts connected the vessels and rays; vasicentric parenchyma with lozenge aliform to aliform confluent arrangement; moderately broad, multiseriate rays and the presence of prismatic crystals in rays were the characteristic feature of the wood. Leaves were hypostomatic and the stomata were cyclocytic with four or more subsidiary cells surrounding the guard cells. Stellate, glandular trichomes were present in upper epidermis, while unicellular, simple, glandular, tufted trichomes were present in the lower epidermis. The increase in global trade has resulted in over-exploitation of forest resources, and hence in the present context, the identification and traceability of wood is highly crucial. The outcomes of this study are supposed to help in the identification of wood of Sal through their anatomical study.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89877695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Mandal, Anisha Pandey, Prasamsha Pant, Seema Sapkota, P. Yadav, D. P. Bhandari
{"title":"Formulation of Herbal Tea from Nepalese Medicinal Plants: Phenolic Assay, Proximate Composition and In-vivo Toxicity Profiling of Medicinal Plants with Nutritive Benefits","authors":"A. Mandal, Anisha Pandey, Prasamsha Pant, Seema Sapkota, P. Yadav, D. P. Bhandari","doi":"10.3126/bdpr.v20i01.56603","DOIUrl":"https://doi.org/10.3126/bdpr.v20i01.56603","url":null,"abstract":"Herbal tea, also known as tisane, is a beverage made from the infusion or decoction of plant material in hot water. True tea comes from the Camellia sinensis plant, while tisane (herbal tea) comes from a water-based infusion of herbs, spices, flowers, leaves etc. This study aimed to formulate and determine the nutraceutical value (proximate analysis), phytochemical value (total phenolic content) and in-vivo toxicity of the different medicinal plants used to prepare three different types of herbal tea formulations. Natural Product Research Laboratory (NPRL)-1 [Asparagus officinalis L., Phyllanthus emblica L., Mentha piperita L., Elettaria cardamomum (L.) Maton and Camellia sinensis (L.) Kuntze], NPRL-2 [Ocimum tenuiflorum L., Bergenia ciliate (Haw.) Sternb., Elettaria cardamom (L.) maton and Camellia sinensis (L.) Kuntze] and NPRL-3 [Salvia rosmarinus Spenn., Cymbopogon citratus (DC.) Stapf, Senegalia catechu (L.f.) P.J.H.Hurter & Mabb. Elettaria cardamomum (L.) Maton and Withania somnifera (L.) Dunal] herbal tea formulations were prepared from these selected medicinal plants. They were respectively tested for their properties. All the plants included were highly nutritional and none were found toxic. The results suggested that herbal tea made up of these potent plants’ parts can be a good choice for health-promoting benefits. These formulations could further be studied for their other beneficial activities.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86060447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cladistic Analysis of Morpho-anatomical Data of Subtribe Coelogyninae of Nepal Himalaya","authors":"D. Bajracharya, Gita Dongol, S. Rajbhandary","doi":"10.3126/bdpr.v20i2.56981","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.56981","url":null,"abstract":"A cladistic analysis of subtribe Coelogyninae based on 10 morphological and 19 anatomical characters of 20 species of Coleogynae, Pholidota, Panisea and Pleione from Nepal was done. Most parsimonious tree were traced using computer assisted software NONA, Winclada (123 trees with CI = 35 and RI = 57). The analysis confirms the monophyletic origin of the subtribe Coelogyninae and species assorted into two different clades. Clade I is separated by round to oval mesophyll cell shape, such as in the species of Otochilus, Panisea and Pleione. Whereas the clade II is separated by stomatal index value 10-15. The results of cladistic analysis suggest that the subtribe Coelogyninae is monophyletic.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86160662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tulasi Shiwakoti, N. Thapa, Saroj Basnet, A. Tiwari
{"title":"Growth Response of Pinus wallichiana to Changing Climate in Temperate Regions of Central Nepal","authors":"Tulasi Shiwakoti, N. Thapa, Saroj Basnet, A. Tiwari","doi":"10.3126/bdpr.v20i01.56595","DOIUrl":"https://doi.org/10.3126/bdpr.v20i01.56595","url":null,"abstract":"Climate influences both the structure and function of the forest ecosystem. A dendrochronological study was carried out in temperate forest at Patney Bhanjyang Community Forest of Kavrepalanchok district in Central Nepal to verify and record the impact of climate on radial growth by using the tree cores of Pinus wallichiana. A total of 60 tree cores were collected by using increment borer and annual ring widths were analyzed by using the standard dendrochronological technique. We produced a 74-year-old tree ring width chronology of P. wallichiana from the region and examined the critical climatic factor for radial growth and the temporal trend of Basal Area Increment (BAI). Our results showed that the tree radial growth was not influenced by maximum temperature. However, it was positively correlated with the minimum temperature of the previous year September (0.30) indicating that cool previous September is favorable for radial growth. Similarly, the radial growth of Pine showed a positive correlation with the total rainfall of January (0.33) and March (0.33), showing that enough moisture in the very early growing season contributes positively to radial growth. We found a decline in BAI after 2000 AD until 2005, although BAI was relatively stable despite the decreasing trend of rainfall. We did not find the impact of climatic factor for the decline in BAI. However long-term study of different climatic, ecological and anthropogenic influences are necessary to know more about the growth–climate relationship of P. wallichiana in temperate forests of Nepal.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":"2013 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86434006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chetana Khanal, Sudhir Neupane, Madhu Shudan Thapa Magar
{"title":"Ethnobotany of Medicinal Plants Used by Kathariya (Tharu) Community in Kailali District, Nepal","authors":"Chetana Khanal, Sudhir Neupane, Madhu Shudan Thapa Magar","doi":"10.3126/bdpr.v20i2.57038","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.57038","url":null,"abstract":"This study aims to document the traditional knowledge on medicinal use of plants by Kathariya (Tharu) community in Kailali district. Kathariya is one of the sub-group of the Tharu community whose culture and language is slightly different from other sub-groups. Traditional healers Guruwa of Kathariya community from five study sites viz. Sisaiya, Tappa, Udasipur, Pawera and Joshipur of Kailali were identified as key informants. Exploratory research design was adopted and interview method was used to collect the primary information through Semi-structured open-ended questionnaire. Altogether 70 species of plants used for medicinal purposes were documented. Sixteen species were found useful in cuts, wounds and swelling which is one of ten health issues categorized in the present study. Thirteen and Twelve species were recorded to be used in abdominal disorders and skin diseases respectively. The community usually mixes powder in measurement of two and half grains of each Barley and Black Pepper during preparations of medicine for oral route of consumption. Based on habit, highest number of plant species belongs to herbs (30 species). Among five study sites, highest number of species was recorded in Udasipur with 35 species. Regarding the parts of plant used, roots and leaves were found to be used often equally, which were represented by 24 species each. Achyranthes aspera, Euphorbia hirta, Azadirachta indica and Clerodendrom infortunatum were the most frequently used species. This study generated preliminary but crucial information which may open the avenue for utilization of biological resources. Further research leading to bio-prospecting is needed to utilize these resources.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75926332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Insights from Local Community on Changing Availability of Non-Timber Forest Products under Climate Change in Panchadeval Binayak Municipality, Achham District, West Nepal","authors":"Sabina Shahi, H. Paudel, Shiba Raj Ghimire","doi":"10.3126/bdpr.v20i2.57048","DOIUrl":"https://doi.org/10.3126/bdpr.v20i2.57048","url":null,"abstract":"Local people in the hilly area depend upon different varieties of non-timber forest products (NTFPs) such as foods, medicines, spices, etc. for income generation, except timber. This paper highlights the perception of local people over current situation including status of availability of NTFPs under changing climate. Data were collected by using questionnaire survey and key informant interview (KII) in 9 wards of Panchadeval Binayak Municipality during April, 2022. Altogether 225 interviews were taken with the oldest family members of each household. Total 89 species of NTFPs were noted. Majority of species were used for medicinal purpose (53.92%), followed by edible (30.33%) whereas 15.73% species were used for fodder, fuelwood and other purposes. Climate change and availability of NTFPs were analyzed according to people’s perception. The study showed that about 96% of respondents agreed to increase in temperature, 84.83% believed in decrease of rainfall, whereas13.33% agreed the rainfall to be unpredictable. Due to those climatic changes, the availability of NTFPs is predicted to decrease and the dependency of people on NTFPs is in critical condition in this municipality. Therefore, it is urged to develop some climate change coping strategies at the local and national level which will assure the NTFPs availability for the local people.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":"98 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79260917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Madhu Ghimire, H. Aryal, P. B. Lakhey, Rajeshwor Ranjitkar, Yagya Raj Bhatta
{"title":"Toxicity Test of Some Selected Wild Mushrooms of Nepal","authors":"Madhu Ghimire, H. Aryal, P. B. Lakhey, Rajeshwor Ranjitkar, Yagya Raj Bhatta","doi":"10.3126/bdpr.v20i01.56602","DOIUrl":"https://doi.org/10.3126/bdpr.v20i01.56602","url":null,"abstract":"People have been using wild mushrooms in their diet and also as sources of income, but most of them do not have the proper knowledge to distinguish edible mushrooms from poisonous ones. The main objective of this research is to determine and document toxicity of some selected wild mushrooms of Nepal. Samples were collected based on their use practices by the local communities, representing different ecological belts. The tests were carried out following the protocol described in ‘Test Guideline no. 425: Acute Oral Toxicity: Up-and-Down Procedure’. Mice (Mus musculus) were administered with aqueous solution of each sample in a single dose by gavage using a stomach tube on the basis of their body weight and were observed for the signs of toxicity. The tested samples of Boletellus emodensis, Caloboletus calopus, Daedalea quercina, Lactifluus volemus, Lyophyllum decastes, Macrolepiota albuminosa, Phellodon niger, Phylloporus bellus, Russula delica, R. emetica, R. senecis, Strobilomyces strobilaceus, Termitomyces eurrhizus, T. le-testui, T. mammiformis, T. robustus, T. striatus f. brunneus, T. straitus f. pileatus, Trametes vernicipes and Trichaptum biforme revealed LD50 value > 2000 mg.kg-1 body weight indicating that they may be harmful if swallowed . However, in case of Hapalopilus rutilans, LD50 value of 1212 mg.kg-1 body weight was observed confirming that it is harmful when swallowed.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89747663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Grass Flora along Altitudinal Gradient of the Phulchoki Hill, Central Nepal","authors":"Anjana Kharbuja, S. Rajbhandary","doi":"10.3126/bdpr.v20i01.56579","DOIUrl":"https://doi.org/10.3126/bdpr.v20i01.56579","url":null,"abstract":"Phulchoki hill on the southern part of Kathmandu Valley (1550-2750 m) presents a unique opportunity to study the diversity of grasses along the altitudinal gradient. During the present study, 73 species of grasses belonging to 5 sub families, 16 tribes and 48 genera were recorded, out of which 28 species are new to this area. Among the recorded species 89% of the species were terrestrial, 7% lithophytes and remaining 4% aquatic. The lowest elevation (1550-1950 m) has highest diversity with 49 species whereas the topmost band (2351-2750 m) has least diversity with only 19 species. The total species richness of grass has decreasing trend along the altitudinal gradient of species, with r2 =0.97 and p=0.04, which indicates significant relation.","PeriodicalId":16849,"journal":{"name":"Journal of Plant Resources and Environment","volume":"59 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85575803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}