Journal of Plant Growth Regulation最新文献

{"title":"The Impacts of Chitosan on Plant Root Systems and Its Potential to be Used for Controlling Fungal Diseases in Agriculture","authors":"Pipob Suwanchaikasem, Alexander Idnurm, Jamie Selby-Pham, Robert Walker, Berin A. Boughton","doi":"10.1007/s00344-024-11356-1","DOIUrl":"https://doi.org/10.1007/s00344-024-11356-1","url":null,"abstract":"<p>Chitosan is a natural elicitor, used for stimulating plant growth and inducing plant defense. However, due to difficulty in monitoring root growth and activity, the effects of chitosan treatment on plant root systems have been less studied as compared to plant shoot parts that include leaves, seeds, and fruits. This results in an indefinite outcome of the benefits of chitosan on plant roots. Therefore, this review aims to evaluate the effects of chitosan treatment on root growth and defense responses based on current evidence. Interestingly, many studies have demonstrated that chitosan can induce plant root defense systems, yet conversely inhibiting root growth. The effects were most clearly observed from studies using liquid or solid media as substrates, while the results from the studies using soil were inconclusive and require additional investigation to observe the effects of environmental factors. In addition, root chitosan treatment showed variable effects on shoot growth, where low chitosan concentrations tend to promote shoot growth, but high chitosan concentrations may affect shoot development. Additionally, this review discusses the potential methods of chitosan application onto plant roots. Water insolubility of chitosan is likely a major issue for root treatment. Chitosan can be dissolved in acids, but this could induce acidity stress in plant roots. Modified versions of chitosan, such as chitosan nanoparticles, carboxylated chitosan, and graft chitosan copolymers have been developed to improve solubility and functionality. Chitosan nanoparticles can also be used to encapsulate other biocontrol agents to augment biological effects on plant defense. In conclusion, root chitosan treatment could help to promote plant defense and prevent root infections, abating the uses of chemical fungicides in agriculture. However, further research is required to monitor the impact of root chitosan treatment on long-term plant growth in order to gain multifaceted information to maximize the effectiveness of root chitosan application.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":"41 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141189644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Drought-Mitigating Rhizobacterium, Bacillus endophyticus J13, Modulates Soil Moisture Content Under Drying Conditions, Precluding the Necessity of Drought-Mediated Signaling in Arabidopsis thaliana","authors":"Raunak Sharma, Atish Kumar Behera, Raja Gopalan Nenmeli Sampathkumar, Sridev Mohapatra","doi":"10.1007/s00344-024-11352-5","DOIUrl":"https://doi.org/10.1007/s00344-024-11352-5","url":null,"abstract":"<p>The use of environmental-stress resilient plant growth promoting rhizobacteria (PGPR) offers an organic solution to sustainable agriculture, under rapid climate change. Our laboratory has previously reported the drought-ameliorating property of an exopolysaccharide-secreting PGPR strain, <i>Bacillus endophyticus</i> J13 on <i>Arabidopsis thaliana</i>. In this study, <i>A. thaliana</i> roots were inoculated with J13 under well-watered and water-stressed conditions (under controlled plant growth conditions). To understand the mechanism of drought amelioration, impact of J13 on plant ABA biosynthesis and signaling was analyzed. It was found that the ABA levels in water-stress, inoculated plants were lower than the water-stressed plants, without inoculation. Also, the expression of ABA biosynthesis genes: <i>NCED3, AAO3</i> and <i>ABA2</i>, was downregulated in inoculated treatments under water-stress as compared to non-inoculated, water-stress treatments. J13 did not cause any modulation in the expression of ABA-dependent signaling gene <i>SnRK2</i> and the ABA-independent signaling gene, <i>DREB2A</i>, under water-stress in Arabidopsis shoots. On treating the bacteria with EDTA (which negatively impacts biofilm levels), we observed that J13 fails to impart stress tolerance to plants under water deficit conditions. The soil moisture content in soil adhered to roots and exopolysaccharide (EPS) content was significantly higher in the plants under water-stressed, inoculated treatments than the non-inoculated plants. This study unravels the mechanism of drought amelioration by J-13 on Arabidopsis by modulating soil moisture content through EPS secretion, thereby eliminating the need for enhanced ABA biosynthesis and signaling.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":"41 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141189637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding the Flower-Anthesis Mechanism in Cestrum nocturnum and Cestrum diurnum","authors":"Shafquat Fakhrah, Nasreen Bano, Soumit Kumar Behera, Krishna Kumar Rawat, Alka Kumari, Chandra Sekhar Mohanty","doi":"10.1007/s00344-024-11369-w","DOIUrl":"https://doi.org/10.1007/s00344-024-11369-w","url":null,"abstract":"<p>The present study investigates the flower-anthesis mechanism in two closely related plant species, <i>Cestrum diurnum</i> and <i>Cestrum nocturnum</i>. These plants are morphologically similar, but exhibit distinct flowering times: <i>C. diurnum</i> flowers during the day, whereas <i>C. nocturnum</i> blooms at night. This contrast in flowering time makes them ideal subjects for this study. Physiological parameters like photosynthetic rate, floral development, and chlorophyll content were analyzed along with transcriptome sequencing. Transcripts related to blooming time were analyzed using de novo sequencing analysis, with emphasis on photoperiod, autonomous, circadian clock, and vernalization pathways. Gene Ontology and KEGG pathway enrichment analysis revealed differences in morphology and physiology. Transcription factors (<i>bHLH, ERF, MYB,</i> and <i>C2H2</i>) involved in regulating flowering time were identified between these two species. The delayed flowering of <i>C. nocturnum</i> was validated by qRT-PCR, which showed the involvement of <i>FT</i>, <i>FLC</i>, and <i>CO</i> genes. Physical attributes such as larger leaves and higher chlorophyll content were two distinctive characteristics of <i>C. nocturnum</i> attributed to higher <i>PHYA</i> levels. Explaining the different flowering events between these two species, the study implies that <i>C. nocturnum</i> focuses on flower formation, photoperiodic responses, and meristem management, whereas <i>C. diurnum</i> prioritizes growth and development.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":"46 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141189678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UV-B-Priming Combined with the Soil Application of MWCNT Enhances Rice Growth Performance Under Salt Stress","authors":"Jing Ji, Xinya Wang, Gang Wang, Jiaqi Zhang, Wenju Song, Runzhong Wang, Baoying Ma, Tiange Li, Chunfeng Guan","doi":"10.1007/s00344-024-11367-y","DOIUrl":"https://doi.org/10.1007/s00344-024-11367-y","url":null,"abstract":"<p>Soil salinization poses a serious threat to sustainable ecological agricultural development. Most crops are susceptible to salt stress during the growth phase of seed germination and seedling emergence. Seed priming could activate the pre-germination metabolic processes, thereby improving seed germination and seedling growth under abiotic stress conditions. Moreover, multi-walled carbon nanotube (MWCNT), as an important class of carbon nanotubes, has been applied to promote plant growth by enhancing antioxidant defense systems and photosynthetic performance during the seedling stage. In this way, it is worth exploring the combination of pre-germination and post-germination treatments to reduce the damage of salt stress to crops. Therefore, in this study, rice seeds were firstly exposed to UV-B tube in a UV irradiation box for priming treatment (90 min). After emergence, the seedlings were transplanted into pots containing 150 mg L⁻¹ MWCNT and 200 mM NaCl to evaluate the positive effects of nanomaterial on the growth of rice. The results demonstrated that after 28 days of salt stress exposure, the combination of UV-B priming and MWCNT significantly improved the growth performance of rice and minimized the adverse effects of salt stress, as compared with the single UV-B priming or MWCNT treatment, increasing biomass accumulation and the contents of osmoregulation substances, modulating antioxidant enzyme system, improving photosynthetic performance. This study suggested that the integration of seed priming with UV-B and soil application of MWCNT enhanced antioxidant capacity, which might increase photosynthesis and biomass accumulation, thereby improving the salt tolerance of rice.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":"79 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141189679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the Defence Response of Wheat Mutants Developed for augmenting Terminal Heat Stress Tolerance and Improved Grain-Quality","authors":"Ranjeet R. Kumar, Suman Bakshi, Suneha Goswami, Sudhir Kumar, Vinutha Thimmegowda, Sanjay J. Jambhulkar, Gyan P. Mishra, Gyanendra K. Rai, Soora Naresh Kumar, Bhupinder Singh, Gyanendra P. Singh, Viswanathan Chinnusamy, Shelly Praveen","doi":"10.1007/s00344-024-11355-2","DOIUrl":"https://doi.org/10.1007/s00344-024-11355-2","url":null,"abstract":"<p>Terminal heat stress is one of the major problems in wheat growth, yield and grain-quality. Here, we have developed wheat mutant (M₃) for HS-tolerance and improved grain-quality using gamma irradiation [parent-MP3054- C-306/CB.SPRING BW/CPAN2072 (Parentage)]. Biochemical markers based characterization showed wheat mutant to be better in HS-tolerance and grain-quality, as compared to parent. To elucidate the mechanism of thermotolerance in wheat mutant, we performed de novo transcriptome assembly of mutant (M₃), parent (P₃), and mutant exposed to HS (M₃H). We generated 6.3 (P₃-R₁), 6.6 (P₃-R₂), 8.1 (M₃-R₁), 7.1 (M₃-R₂), 7.6 (M₃H-R₁), and 6.1 (M₃H-R₂) million clean reads and identified 3,05,537 genes and 5,88,788 transcripts with an N₅₀ of 1,349 bp. We observed 6,120 upregulated and 4,428 downregulated transcripts (M₃ vs P₃), 11,354 upregulated and 12,408 downregulated genes (M₃H vs P₃) and 4817 upregulated and 9085 downregulated genes (M₃H vs M₃). The high HS-tolerance and improved grain-quality of the wheat mutant was observed due to the upregulation of serine threonine kinase (STK), HSP20, SOD, ATP-binding cassette (ABC) transporters, heat shock transcription factor (HSF), and calcium dependent protein kinase (CDPK) and stability of starch synthase, sucrose synthase, and debranching enzyme. Gene Ontology analysis showed ‘ATP-binding’ to be most enriched category. The carbon assimilatory pathways (photosynthesis and starch biosynthesis) were observed most altered under terminal HS. The developed mutant can be further utilized as donor in wheat breeding program to develop ‘climate-smart’ crop.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":"22 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141168964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Analysis Reveals the Role of Sucrose and Starch Metabolism and Systemic Homeostasis in Seed Abortion in Distant Hybrids of Peony","authors":"Dan He, Mingxing Zhang, Songlin He, Chao Hua, Haonan Guo, Yihong Chang, Yang Liu, Zheng Wang, Yiping Liu","doi":"10.1007/s00344-024-11334-7","DOIUrl":"https://doi.org/10.1007/s00344-024-11334-7","url":null,"abstract":"<p>Peony is a flower species with great ornamental value. Germplasm innovation is urgently needed due to the rapid development of peony industrialization around the world. However, the occurrence of seed abortion restricts the hybrid breeding programs of peony, significantly impeding the development of new cultivars. Hence, gaining a comprehensive understanding of the molecular mechanism of seed abortion in peony will provide opportunities to overcome this challenge of seed abortion in hybrid breeding programs for peony. In this study, we conducted physiological and transcriptomic analyses on peony seeds at three distinct time points (14d, 18d, and 28d) after pollination, aiming to investigate the mechanism underlying seed abortion. The results indicated that the regulation of seed abortion in peony involves multiple metabolic pathways, which are regulated by a plenty of differentially expressed genes. Specifically, we successfully identified 25 differentially expressed genes involved in sucrose catabolism as well as starch synthesis and catabolism, indicating sucrose and starch metabolic pathways play significant roles in the occurrence of seed abortion during peony hybridization. Six genes associated with sucrose transport and catabolism consistently exhibited the same expression pattern across different time points. This suggested that the modifications in enzyme activities contribute to the change of starch and sucrose contents during seed development. Additionally, the activities of peroxidase and superoxide dismutase changed during seed abortion. Certain proteins encoded by genes associated with starch and sucrose metabolism may potentially interact with SOD-related proteins. However, the precise mechanisms of these interactions require further validation. Finally, we identified several transcription factor families that are also implicated in seed abortion, highlighting the intricate gene regulatory network associated with this process. These results provide a theoretical foundation for determining the optimal period of seed embryo salvage and present a novel approach to enhance the seed set rate of peony.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":"22 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141153760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Plant Drought Tolerance","authors":"Muhammad Farooq, Abdul Wahid, Noreen Zahra, Muhammad Bilal Hafeez, Kadambot H. M. Siddique","doi":"10.1007/s00344-024-11351-6","DOIUrl":"https://doi.org/10.1007/s00344-024-11351-6","url":null,"abstract":"<p>Drought stress (DS) is a recurrent and severe meteorological challenge affecting agricultural regions globally, leading to significant shifts in plant species distribution and substantial reductions in crop yields. The seasonal occurrence, intensity, and duration of DS vary in response to changing climatic conditions. Plants deploy various physio-biochemical and anatomical alterations to combat such conditions, including stomatal closure, modified root growth and architecture, shifts in metabolic pathways, and altered physiological responses. Moreover, metabolic shifts and physio-chemical adaptations under DS often shorten plant life cycles, reducing yields. Nevertheless, the impact of DS on plants depends on the soil water gradient, precipitation duration and degree, plant species, and developmental stage. This review elucidates the complex effects of DS on trait variations, highlighting the current knowledge on advances in genetic engineering, breeding, and agronomic approaches and exploring potential strategies to increase yield and develop drought-resistant crops.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":"31 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141063698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Survey of the DELLA Transcription Factors in Cucumber and Functional Characterization of CsDELLA1 in Regulation of GA-Mediated Growth and Development","authors":"Yong Zhou, Jingju Chen, Yaqi Hu, Lingdi Xiao, Zhaoyang Hu, Yuelong Zhou, Shiqiang Liu","doi":"10.1007/s00344-024-11308-9","DOIUrl":"https://doi.org/10.1007/s00344-024-11308-9","url":null,"abstract":"<p>DELLAs are known as negative regulators of gibberellin (GA) signaling pathway and control many aspects of plant development. In recent years, <i>DELLA</i> family genes were widely studied in many plant species, but little was known about the biological functions of <i>DELLA</i> genes in cucumber. In this study, genome-wide identification of the <i>DELLA</i> gene family members was performed (<i>CsDELLA1–4</i>), and their phylogenetic relationship, conserved motif, gene structure, <i>cis</i>-regulatory elements, and expression pattern were systematically analyzed. Amongst them, <i>CsDELLA1</i> encoded a protein-harbored DGLLA, LERLE, and less-conserved TVHYNP motif in its N-terminal DELLA domain, implying the possible role of <i>CsDELLA1</i> in GA-regulated growth and development. As anticipated, silenced <i>CsDELLA1</i> by virus-induced gene silencing resulted in increased plant height and early flowering phenotypes. In contrast, overexpression of <i>CsDELLA1</i> in <i>Arabidopsis</i> resulted in dwarf, late flowering, and inhibited hypocotyl elongation phenotypes, indicating that overexpression of <i>CsDELLA1</i> inhibited the GA response in <i>Arabidopsis</i>. The transgenic plants also produced more buds and shorter siliques than the wild-type (WT) plants, and displayed a significantly longer primary root length than that of WT under exogenous GA₃ treatment. Collectively, these results indicated that the <i>CsDELLA1</i> might play important roles in regulation of GA-mediated growth and development.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":"66 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140942047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jasmonic Acid (JA) in Plant Immune Response: Unravelling Complex Molecular Mechanisms and Networking of Defence Signalling Against Pathogens","authors":"Rajib Roychowdhury, Alkesh Hada, Sabarni Biswas, Sapna Mishra, Manas Ranjan Prusty, Soumya Prakash Das, Shatrupa Ray, Ajay Kumar, Umakanta Sarker","doi":"10.1007/s00344-024-11264-4","DOIUrl":"https://doi.org/10.1007/s00344-024-11264-4","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Jasmonic acid (JA) and its derived compound ‘jasmonates’ are a class of potent phytohormones that play pivotal roles in plants’ physio-biochemical processes during growth and development. With the onset of the 21st century, concurrent climate changes all over the world have drastically affected plants’ adaptability and survivability against (a)biotic stressors. Such climatic instabilities trigger a variety of pathogens (mainly fungi, bacteria, and viruses) to create disease pressure by affecting plant health and the immune barrier. JA is crucial for plant immune response through their biosynthetic pathways, involvement in signalling cascades and corresponding cross-talk with other phytohormones like salicylic acid (SA), ethylene (ET) and abscisic acid (ABA). In this perspective, the endogenous role of JA lies behind the process of cellular central dogma at the molecular level. The genes and gene products, like transcription factors (TFs) associated with JA biosynthesis and signalling, impart some sort of regulation on plant immune response by activating systemic and localized signalling, pathogenesis-related (PR) genes and proteins, phytoalexins, modulating pathogen effectors, and interacting with host proteins. In addition, the external application of JA can also significantly lead to stimulating the immune circuit of plants through gene expression and protein interaction. In this way, JA can boost a plant’s immune system through systemic acquired resistance (SAR) and induced systemic resistance (ISR). This could be an idea to understand the hormonal interplay in plant immune response to various types of pathogens and provide the resistance mechanism against yield and quality losses.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":"124 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140942281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytohormone Profiling of Malus domestica and Chenopodium murale Hairy Root Exudate: Association with Allelopathic Effects","authors":"Slavica Ninković, Václav Motyka, Mariana Stanišić, Dijana Smailagić, Branka Živanović, Petre I. Dobrev, Nevena Banjac","doi":"10.1007/s00344-024-11328-5","DOIUrl":"https://doi.org/10.1007/s00344-024-11328-5","url":null,"abstract":"<p>Compounds exuded from roots play a key role in regulating plant allelopathic interactions. However, phytochormone profiling of root exudates and their contribution to an overall allelochemical activity of specific plant species is neglected topic in allelochemical research. Hairy root growth media of two different species, the fruit tree species <i>Malus</i> × <i>domestica</i> Borkh. and the herbaceous weed species <i>Chenopodium murale</i> L. were collected and analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). We found that most of the phytohormones exuded by the hairy roots of <i>C. murale</i> and <i>M. domestica</i> were associated with the acidic fraction (96.8% and 98.9%, respectively), including 2-oxindole-3-acetic acid, phenylacetic acid, salicylic acid (SA), benzoic acid (BzA), and abscisic acid, with SA and BzA being the most abundant, while those associated with the basic fraction, including cytokinins and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, accounted for only 1% of the plant growth substances detected in both species. Exogenous application of 0.2 µM SA, which was released from the hairy roots of <i>C. murale</i> and accumulated in the culture media for four weeks, significantly impaired hairy root growth of <i>M. domestica</i> and also shoot and root growth of Arabidopsis seedlings. The disruptive effect of 0.2 µM SA on the membrane potential of <i>M. domestica</i> hairy root and Arabidopsis root cells was determined. The data obtained could be useful for planning further studies aimed at clarifying the contribution and role of exuded phytohormones to the overall allelopathic potential of these two plant species.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":"218 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140930055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}