Journal of Plant Diseases and Protection最新文献

{"title":"Biochemical dynamics during development of insect-induced plant galls: a review","authors":"Preeti Mishra, Parmila Saini, Vidya Patni","doi":"10.1007/s41348-024-00931-z","DOIUrl":"https://doi.org/10.1007/s41348-024-00931-z","url":null,"abstract":"<p>Insect-induced galls are regulated outgrowths of plant tissues that result from unique and mutual interactions between host plants and gall-inducing insects. The insects, mainly belonging to the orders Hemiptera, Hymenoptera, Thysanoptera, and Diptera, induce gall formation through activities such as oviposition, feeding, secretions, and chewing. These activities trigger morphogenetic and physiological factors, leading to the development of distinct forms of galls. It is noteworthy that about 90% of gall-inducing insects exhibit host specificity. This review investigates the biochemical and metabolic changes that occur when plants and insects interact. These insects use enzymes such as polygalacturonase, pectinesterase, cellulase, and proteinase present in their saliva to assist in breaking down the plant's cuticle and cell wall during the infestation. This process disrupts the subcellular environment, resulting in a chemical shock at the infestation sites. Stressful conditions stimulate the generation of action potential in plants, activating channels and causing faster depolarization of the plasma membrane. Additionally, under stress, plants may generate high levels of reactive oxygen species (ROS), leading to an oxidative burst at sites of infection. ROS triggers necrosis through a hypersensitive response. Gall-inducing insects disrupt the normal metabolism of host plants, resulting in a series of biochemical changes and metabolic imbalances. These changes promote the formation of new plant tissues, which require the production of plant growth hormones and ultimately alter the host plant's phenotype.</p>","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"18 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141193213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symptoms and disease virulence assessment in commercial pepper cultivars caused by Pepper chat fruit viroid","authors":"Thitichat Keyata, Samabhorn Sinhabandhu, Kanungnit Reanwarakorn","doi":"10.1007/s41348-024-00943-9","DOIUrl":"https://doi.org/10.1007/s41348-024-00943-9","url":null,"abstract":"<p>The symptoms caused by viroids differ, ranging from asymptomatic to mild-or-severe symptoms. Pepper plant symptoms caused by the <i>Pepper chat fruit viroid</i> (PCFVd) are mild compared to those affecting tomato plants; however, there is not much more known of the symptomatology on pepper plants. Symptoms that could be used for disease virulence assessment in pepper plants were elucidated from 31 commercial pepper cultivars belonging to <i>Capsicum annuum</i> and <i>C. frutescens</i> that had been purchased from agricultural shops. The plants were mechanically sap-inoculated at the seedling growth stage and observed weekly for symptom development, with disease virulence evaluations performed at 4, 8, and 12 weeks post inoculation. Infection of all plants was verified based on reverse transcription-polymerase chain reaction, along with visual evidence of growth reduction, including leaf rugosity and leaf size reduction, a narrow canopy of the blocky and elongated fruit shapes for <i>C. annuum</i> and explicit apical stunting with small apical leaves of the elongated fruit type for <i>C</i>. <i>frutescens</i>. The disease virulence assessment was designed based on these symptoms to produce a score with 0–10 disease virulence levels (DVLs). The results showed that the pepper cultivars displayed responses to PCFVd with DVL scores of 1.00–8.00, with no PCFVd transmission being recorded from seeds to seedlings for the 3 test cultivars. This finding indicated that the genetic resources of pepper cultivars against PCFVd were as low as 1.00 DVL. However, the low DVL pepper cultivars could provide an inoculum source to other susceptible plants via mechanical transmission.</p>","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"36 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141167040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of long-read PCR amplicon sequencing to study the evolution of resistance to zoxamide, oxathiapiprolin and complex III inhibitors in French Plasmopara viticola field populations","authors":"S. Cherrad, B. Gillet, J. Dellinger, L. Bellaton, P. Roux, C. Hernandez, H. Steva, L. Perrier, S. Vacher, S. Hughes","doi":"10.1007/s41348-024-00939-5","DOIUrl":"https://doi.org/10.1007/s41348-024-00939-5","url":null,"abstract":"<p>Molecular methods are one of the most effective tools to monitor fungicide resistance. Long-read sequencing is an emerging technology in the field of plant pathology. We developed a PCR-based Oxford Nanopore Technologies amplicon sequencing method allowing the simultaneous detection and quantification of Plasmopara viticola variants conferring fungicide resistance to complex III inhibitors, zoxamide and oxathiapiprolin in the same vineyard population. Analysis of cyt b gene variants in natural <i>P. viticola</i> populations showed that almost all samples (23 out of 24 populations) collected in France contain variants G143A, S34L and/or E203-DE-V204 insertion. In the analysed populations, only cyt b reads with both substitutions S34L and G134A were detected at significant levels, suggesting the selection of resistant strains to both QoI fungicides and ametoctradin. French <i>P. viticola</i> population P36 with low sensitivity to oxathiapiprolin did not contain oxysterol binding protein sequences with both variants G770V and N837I, suggesting the presence of two different genotypes of <i>P. viticola</i> strain in this population. Zoxamide insensitivity associated with <i>β</i>-tubulin variants carrying the C239S substitution was detected in Italian vineyard populations but not in France.</p>","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"42 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141193220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The nearly complete genome sequence of clade B, wheat streak mosaic virus isolate from Türkiye","authors":"Havva Ilbağı, Rick Masonbrink, Wyatt Allen Miller","doi":"10.1007/s41348-024-00942-w","DOIUrl":"https://doi.org/10.1007/s41348-024-00942-w","url":null,"abstract":"<p>Wheat streak mosaic virus is one of the most widespread viruses in cereal crops, causing severe losses and dramatically affecting worldwide wheat production. Currently, four distinct clades of WSMV have been grouped and named: A (Mexico), B (Europe, Asia, Russia, the USA), C (Iran), and D (The USA, Argentina, Brazil, Australia, Canada, Türkiye). In this study, we determined the nearly complete and partial nucleotide sequences of WSMV type B isolates collected from wheat found in the European part of Türkiye using high throughput sequencing. Excluding the poly(A) tail, the genome of isolate S34Edirne (GenBank acc.no. MZ405098) consisted of 9360 nucleotides and contained a single large open reading frame encoding a polyprotein of 3033 amino acids. The partial sequence of WSMV in wheat isolate (GenBank acc.no. ON364116) is 3348 nt long. The characteristic lack of a GGA (Gly₂₇₆₁) codon within coat protein (CP) of the polyprotein of S34Edirne complete nucleotide sequence is typical for the clade B, WSMV-ΔE isolates, which are widely found throughout the European continent. Sequence comparisons revealed that WSMV Turkish wheat isolate is the most closely related to Czech isolate, with highly similar nucleotide and amino acid identities at 98.83 and 99.13%, respectively. The result of this study indicates that the nearly complete and partial genome sequences of S34Edirne and S34_Edirne24 isolates should be placed into clade B of the European WSMV-ΔE isolates.</p>","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"76 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141193214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of two different experimental environments for resistance screenings for the leafhopper-transmitted wheat dwarf virus in wheat","authors":"Anne-Kathrin Pfrieme, Andreas Stahl, Klaus Pillen, Torsten Will","doi":"10.1007/s41348-024-00929-7","DOIUrl":"https://doi.org/10.1007/s41348-024-00929-7","url":null,"abstract":"<p>Wheat dwarf virus (WDV) causes high yield losses in wheat and other cereals and is therefore an important pathogen transmitted by the leafhopper <i>Psammotettix alienus</i>. Climate change will increase infections by insect-transmitted viruses due to the increasing spread of vectors. In the context of integrated pest management, the cultivation of WDV-resistant/tolerant varieties is an effective way of controlling WDV. Evaluation of tolerant/resistant genotypes is based on inoculation with viruliferous leafhoppers and subsequent phenotyping in gauze houses under semi-field conditions. For successful screening, it is important to ensure the uniform and reproducible inoculation of plants. Abiotic conditions, particularly temperature, have a critical influence on inoculation success, and thus, variations in infection rates were observed within and between previous replicates in the field. Furthermore, the leafhopper population reared in the greenhouse has to be reestablished after each infection, which delays the screening process. We addressed these issues by developing an improved inoculation assay in which plants are inoculated in small infection hoods in the greenhouse before being planted out in gauze houses. This procedure allows optimal environmental conditions for WDV infection of test plants and allows the plants with WDV infection to develop under natural environmental conditions for symptom scoring. In addition, the viruliferous leafhoppers were recollected from the test plants after infection, allowing a sustainable use of the insects. The method thus enables more reliable phenotyping by increasing infection success and testing a greater number of genotypes in a shorter time.</p>","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"51 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141193221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cap-snatching mechanism-mediated unveiling of the transcriptional initiation sites of two distinct begomoviruses","authors":"Muhammad Arif","doi":"10.1007/s41348-024-00941-x","DOIUrl":"https://doi.org/10.1007/s41348-024-00941-x","url":null,"abstract":"<p>Significant economic losses are inflicted by plant viruses, which pose a risk to sustainable agriculture. The proliferation of novel viral diseases is predominantly attributable to factors such as climate change, international trade, and the rapid evolutionary capabilities of viruses. Begomoviruses are a major group of plant-infecting viruses that pose an imminent threat to global agriculture by causing devastating viral diseases in many crop species. The transcriptional start sites (TSSs) of many plant viruses are typically found in the intergenic region (IR), which is the non-coding (NC) area between the viral genes. The promoters play a crucial role in initiating the transcription process by aiding in the recruitment of cellular transcription machinery. The TSSs are precise nucleotide sequences where RNA polymerase initiates the transcription process. The primary objective of this study was to determine the total number of TSSs for two devastating begomoviruses, family: Geminiviridae, <i>Cotton leaf curl Multan virus</i> (CLCuMuV) and <i>Ageratum yellow vein mosaic virus</i> (AYVMV), using the cap-snatching method in conjunction with one heterologous plant virus. These two begomoviruses, along with their infectious clones, were intentionally infected with selected heterologous plant virus in <i>N. benthamiana</i> plants. The identification of the 5′ ends of heterologous viral mRNA was accomplished by employing high-throughput sequencing to assess the capped RNA leaders (CRLs). The determination of the 5′ termini of suspected begomoviral mRNAs was achieved by aligning the collected CRLs of heterologous virus with the genome of each begomovirus, taking into account only those that were a perfect match with the begomoviral genome. In this study, the TSSs of both begomoviruses were identified via complementary approach. The utilization of high-throughput sequencing for both begomoviruses has facilitated the acquisition of millions of sequences. Comprehending the TSSs and promoter components of begomoviruses is crucial for understanding their ability to cause disease, their interactions with host organisms, and for developing effective methods to manage and control the diseases, they inflict on economically significant crop plants.</p>","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"68 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141167039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic strategies for sustainable crop protection: harnessing soil solarization and biofumigants to combat damping-off pathogens in Solanaceous vegetable crops","authors":"Shavnam, Harender Raj","doi":"10.1007/s41348-024-00916-y","DOIUrl":"https://doi.org/10.1007/s41348-024-00916-y","url":null,"abstract":"<p>Field experiments were conducted in the summers of 2019 and 2020 to study the effects of soil solarization, biofumigants derived from cauliflower leaves and combinations of those treatments on population densities of soil pathogens <i>Pythium ultimum</i>, <i>Fusarium oxysporum</i>, <i>Rhizoctonia solani</i>, and <i>Sclerotium rolfsii</i> causing damping-off disease in major solanaceous crops. Nylon mesh bags containing mass culture of <i>P. ultimum, F. oxysporum, R. solani</i>, and <i>S. rolfsii</i> were buried underneath the soil at 5 and 10 cm depth. Soil solarization was performed over a 40-day period using thin transparent polythene sheet of 25 µm (100 gauge) thickness. Maximum soil temperatures recorded at depth of 5 cm were 41.8 °C in solarized soil, respectively; this temperature was 5.4 °C higher than in corresponding non-solarized plot. These nylon sieve bags containing culture of the different pathogens were retrieved after 15, 30 and 40 days of solarization and brought to the laboratory to find out the viability of the pathogens. Integration of soil solarization with cauliflower leaves as soil amendment for the period of 40 days at 5 cm soil depth was found most effective in reducing the viability of <i>P. ultimum, F. oxysporum, R. solani</i>, and <i>S. rolfsii</i> to 5.3, 2.6, 4.6, and 3.6% in comparison with 92.6, 77.3, 83.3, and 80.3 in control, respectively. With the increase in the duration of soil solarization from 15 to 40 days, the potential of the pathogens to cause the disease reduced significantly at 5 cm soil depth and incidence of the damping-off reduced from 63.3 to 16.6% in tomato; 66.6 to 20.0% in chilli and 70.0 to 23.3% in capsicum. Through this integrated approach, the viability of soil-borne pathogens can be minimized, thereby improving the overall plant health.</p>","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"2017 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141149416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disease prevalence, incidence, morphological and molecular characterisation of Lasiodiplodia pseudotheobromae causing collar rot disease on peanut plants in Turkey","authors":"Senem Özkaya, Soner Soylu, Merve Kara, Yusuf Gümüş, Emine Mine Soylu, İbrahim Teke, Oktay Burak Özcan, Deniz Sevilmiş, Yaşar Ahu Ölmez, İsa Bilaloğlu, Işılay Lavkor","doi":"10.1007/s41348-024-00933-x","DOIUrl":"https://doi.org/10.1007/s41348-024-00933-x","url":null,"abstract":"<p>Peanut (<i>Arachis hypogaea</i> L.) holds significant commercial and dietary importance as a major source of edible oil and protein in Turkey. Stem, collar or root rot, caused by several fungal disease agent, are serious soil-borne diseases of peanut. Accurate and precise identification of the disease agent provides fundamental and precise information for integrated plant management. During the period from June to September 2021, symptoms consistent with collar rot disease, including dark-brown stem rot, chlorotic leaves, wilting, and eventual whole plant death, were observed on peanut plants cultivated in the different districts of Osmaniye Province of Turkey. The disease incidence ranged from 8.0 to 45.0% in the inspected fields with an average of 3.4% overall. Twenty-four single-spore representative isolates were obtained from surface-disinfected symptomatic tissues. Morphological characteristics of fungal mycelium, conidial and pycnidial structures on potato sucrose agar (PSA) and water agar (WA) closely resembled those described for <i>Lasiodiplodia</i> spp. All isolates caused typical collar rot symptoms upon artificial inoculation of peanut seedlings. Morphological identification of <i>Lasiodiplodia</i> spp. isolates was corroborated by MALDI-TOF and molecular analyses utilizing sequences from the internal transcribed spacer (<i>ITS</i>)<i>,</i> β-tubulin 2 (<i>tub2</i>) and translation elongation factor-1 alpha (<i>TEF1-α</i>) loci. Phylogenetic analysis confirmed that the representative fungal isolates (MKUBK-B1 and MKUBK-K22) belong to <i>Lasiodiplodia pseudotheobromae.</i> To the best of our knowledge, this is the first report of <i>L. pseudotheobromae</i> infecting peanut plants in Turkey. This work is expected to contribute to previously limited knowledge regarding the host range, incidence and prevalence of <i>L. pseudotheobromae</i> as a soilborne pathogen of peanuts. Due to the potential destructiveness and broad host range of this pathogen, it is essential to develop new strategies to establish more reliable, environmentally sustainable, and cost-effective management approaches for this disease.</p>","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"41 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140940034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Freezing temperature treatments against almond moth, Ephestia cautella (Walker), infestation of date fruits","authors":"Nora Abdul Aziz M. Aqeel, Mureed Husain, Khawaja Ghulam Rasool, Koko D. Sutanto, Richard W. Mankin, Mona A. Alduailij, Abdulrahman Saad Aldawood","doi":"10.1007/s41348-024-00922-0","DOIUrl":"https://doi.org/10.1007/s41348-024-00922-0","url":null,"abstract":"<p>Dates maintain prime importance as sources of income and as symbols of cultural heritage in Saudi Arabia. <i>Ephestia cautella</i> (Walker) is one of the most important insects that attack stored dates. In an effort to safeguard small-scale producers, temperatures of 5 °C (representing typical house refrigerator temperatures), − 14 °C (representing in-house freezer temperatures), and − 25 °C (representing temperatures in small factory freezers) were applied to samples of dates previously infested with <i>E. cautella</i> larvae. Exposure periods of 1, 12, 48, 120, and 240 h were considered to assess the effectiveness of freezing temperatures against stored product insect pests. The temperature of − 25 °C proved to be the most effective, resulting in 100% mortality across all stages after just 1 h of exposure. At 5 °C, the pupal stage exhibited the highest tolerance, with only a mean mortality of 11% after 240 h of exposure. Conversely, at the same temperature, mean mortalities of 89% and 97% were recorded for eggs and larvae, respectively. However, when larvae were placed inside the dates, the mean mortality was only 65% after 240 h at 5 °C, complete larval mortality was achieved in 12 h at − 14 °C and 1 h at − 25 °C. This information can guide strategies for managing <i>E. cautella</i> in storage facilities with limited available resources.</p>","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140939955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol activities of grey mould of grapes with the volatile organic compounds generated by yeast HXMG-1 isolated from grapes","authors":"Ning Li, Bujiang Wang, XinYi Cui, Jing Hou, Na Zhang","doi":"10.1007/s41348-024-00920-2","DOIUrl":"https://doi.org/10.1007/s41348-024-00920-2","url":null,"abstract":"<p>The pathogens are reduced by volatile organic compounds (VOCs) generated by yeasts play an important role in controlling postharvest diseases. The yeast HXMG-1, which works effectively against the grey mould pathogen of grapes (<i>Botrytis cinerea</i>), was evaluated for its potential to generate volatile organic compounds as one of its modes of action. A double Petri dish assay was used to evaluate the effect of VOCs produced by HXGM-1 on mycelial and spore development of the target pathogens. Compared to the control, the VOCs produced by yeast HXMG-1 significantly reduced the growth of mycelium and spore germination of <i>Botrytis cinerea</i>. Specifically, the mycelial growth of <i>Botrytis cinerea</i> was completely restricted and the rate of spore germination of <i>Botrytis cinerea</i> was only 20.11% at a concentration of 1 × 10⁹ CFU/mL. It was also found that the VOCs could significantly inhibit mycelial growth with an inhibition of 82.46% at a concentration of 1 × 10⁸ CFU/mL. The VOCs caused the mycelium to grow curved, resulting in larger mycelial tips, fewer nuclei, and shorter mycelial septum spacing. In vivo tests, noninjure or injure grapes were artificially inoculated with the pathogen hyphal disc followed by biofumigation with VOCs produced by yeast HXMG-1, and the treatments (Wp2 and Wp3) significantly controlled pathogenic infection, confirming the results of in vitro tests. By molecular biological identification based on comparative sequence analysis of the 18S rDNA gene, the HXMG-1 strain was identified as <i>Hanseniaspora uvarum.</i> Through the creation of a phylogenetic tree, HXMG-1 was recognised as a member of the Ascomycota, Hemiascomycota, Yeasts, and <i>Hanseniaspora</i> sp. families. In conclusion, the yeast strain HXMG-1 created VOCs that significantly inhibited the development of <i>Botrytis cinerea</i> on grapes and is expected to be further developed and utilised. This study lays the foundation for the use of <i>Hanseniaspora</i> sp. for biological control of postharvest disease.</p>","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"29 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140940032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}