Journal of pharmacological sciences最新文献

{"title":"Alpha-linolenic acid selectively inhibits the contraction of pig coronary arteries mediated through prostanoid TP receptors","authors":"Kento Yoshioka ,&nbsp;Keisuke Obara ,&nbsp;Mikoto Ozawa,&nbsp;Mayu Kiguchi,&nbsp;Yuri Nakao,&nbsp;Hinako Miyaji,&nbsp;Toma Yamashita,&nbsp;Noboru Saitoh,&nbsp;Yutaka Nakagome,&nbsp;Yoshio Tanaka","doi":"10.1016/j.jphs.2024.06.001","DOIUrl":"10.1016/j.jphs.2024.06.001","url":null,"abstract":"<div><p>We examined the inhibitory effects of α-linolenic acid (ALA) on the contractions of pig coronary arteries. ALA concentration-dependently inhibited the contractions elicited by U46619 and prostaglandin F_2α without affecting those elicited by 80 mM KCl, histamine, acetylcholine, and serotonin. ALA rightward shifted the concentration-response curve of U46619, and Schild plot analysis revealed that ALA competitively antagonized U46619. Furthermore, ALA inhibited the increase in intracellular Ca²⁺ concentration caused by TP receptor stimulation but not that caused by FP receptor stimulation. These results suggest that ALA behaves as a selective antagonist of TP receptors in coronary arteries.</p></div>","PeriodicalId":16786,"journal":{"name":"Journal of pharmacological sciences","volume":"155 4","pages":"Pages 148-151"},"PeriodicalIF":3.5,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1347861324000409/pdfft?md5=784818b35f35232c1de03608f1c7c542&pid=1-s2.0-S1347861324000409-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141279741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure-activity relationship studies of pyrogallol as a calcineurin/NFAT signaling suppressor","authors":"Hiroyuki Mizuguchi ,&nbsp;Tomohira Ito ,&nbsp;Kohei Nishida ,&nbsp;Tomoharu Wakugawa ,&nbsp;Tomohiro Nakano ,&nbsp;Akie Tanabe ,&nbsp;Tomokazu Watano ,&nbsp;Noriko Kitamura ,&nbsp;Osamu Kaminuma ,&nbsp;Katsunori Kimura ,&nbsp;Tatsuya Ishida ,&nbsp;Atsushi Matsunaga ,&nbsp;Kazumi Ohta ,&nbsp;Rina Shimono ,&nbsp;Haruo Kutsuna ,&nbsp;Taiei Yasuda ,&nbsp;Masami Yabumoto ,&nbsp;Yoshiaki Kitamura ,&nbsp;Noriaki Takeda ,&nbsp;Hiroyuki Fukui","doi":"10.1016/j.jphs.2024.06.002","DOIUrl":"https://doi.org/10.1016/j.jphs.2024.06.002","url":null,"abstract":"<div><p>Previously, we have shown that pyrogallol alleviated nasal symptoms and suppressed IL-9 gene up-regulation in allergy model rats by inhibiting calcineurin/NFAT signaling. As pyrogallol has antioxidative activity, it may be responsible for inhibiting calcineurin/NFAT signaling-mediated IL-9 gene expression. However, the relationship between antioxidative activity and suppression of IL-9 gene expression has not been elucidated yet. Here, we conducted the structure-activity relationship studies of pyrogallol and its structurally related compounds to understand the mechanism of IL-9 gene suppression by pyrogallol. 2, 2-Diphenyl-1-picrylhydrazyl radical scavenging assay showed that the antioxidative activity of catechol, resorcinol, phloroglucinol, and gallic acid is 60.1%, 10.4%, 18.8%, and 113.5% of pyrogallol, respectively. Catechol, resorcinol, and phloroglucinol did not suppress NFAT dephosphorylation. Gallic acid suppressed dephosphorylation of NFAT. Gallic acid also suppressed ionomycin-induced up-regulation of IL-9 gene expression with the IC₅₀ value of 82.6 μM. However, catechol, resorcinol and phloroglucinol showed no suppressive activity. In addition, using gallic acid-immobilized beads, we isolated and identified Poly(U)-binding-splicing factor 60 (PUF60) as a pyrogallol binding protein. These results suggest that the antioxidative activity of pyrogallol is not likely to be the mechanism of IL-9 gene suppression. Data also suggest that PUF60 is one of its target molecules responsible for the suppression of calcineurin/NFAT signaling by pyrogallol.</p></div>","PeriodicalId":16786,"journal":{"name":"Journal of pharmacological sciences","volume":"155 4","pages":"Pages 140-147"},"PeriodicalIF":3.5,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1347861324000410/pdfft?md5=987182f899d3f70e134d5521c0594ef1&pid=1-s2.0-S1347861324000410-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141289637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"H2S prevents the disruption of the blood-brain barrier in rats with prenatal hyperhomocysteinemia","authors":"A.V. Yakovlev ,&nbsp;A.S. Detterer ,&nbsp;O.V. Yakovleva ,&nbsp;A. Hermann ,&nbsp;G.F. Sitdikova","doi":"10.1016/j.jphs.2024.05.001","DOIUrl":"10.1016/j.jphs.2024.05.001","url":null,"abstract":"<div><p>Elevation of the homocysteine concentration in the plasma called hyperhomocysteinemia (hHCY) during pregnancy causes a number of pre- and postnatal developmental disorders. The aim of our study was to analyze the effects of H₂S donors –NaHS and N-acetylcysteine (NAC) on blood-brain barrier (BBB) permeability in rats with prenatal hHCY. In rats with mild hHCY BBB permeability assessed by Evans Blue extravasation in brain increased markedly throughout life. Administration of NaHS or NAC during pregnancy attenuated hHCY-associated damage and increased endogenous concentrations of sulfides in brain tissues. Acute application of <span>dl</span>-homocysteine thiolactone induced BBB leakage, which was prevented by the NMDA receptor antagonist MK-801 or H₂S donors. Rats with hHCY demonstrated high levels of NO metabolite – nitrites and proinflammatory cytokines (IL-1β, TNF-α, IL-6) in brain. Lactate dehydrogenase (LDH) activity in the serum was higher in rats with hHCY. Mitochondrial complex-I activity was lower in brain of hHCY rats. NaHS treatment during pregnancy restored levels of proinflammatory cytokines, nitrites and activity of the respiratory chain complex in brain as well as the LDH activity in serum. Our data suggest that H₂S has neuroprotective effects against prenatal hHCY-associated BBB disturbance providing a potential strategy for the prevention of developmental impairments in newborns.</p></div>","PeriodicalId":16786,"journal":{"name":"Journal of pharmacological sciences","volume":"155 4","pages":"Pages 131-139"},"PeriodicalIF":3.5,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1347861324000380/pdfft?md5=f67d33bc8f485841c29607fa19a99e4e&pid=1-s2.0-S1347861324000380-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141134318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supersulfide catabolism participates in maladaptive remodeling of cardiac cells","authors":"Liuchenzi Zhou ,&nbsp;Akiyuki Nishimura ,&nbsp;Keitaro Umezawa ,&nbsp;Yuri Kato ,&nbsp;Xinya Mi ,&nbsp;Tomoya Ito ,&nbsp;Yasuteru Urano ,&nbsp;Takaaki Akaike ,&nbsp;Motohiro Nishida","doi":"10.1016/j.jphs.2024.05.002","DOIUrl":"https://doi.org/10.1016/j.jphs.2024.05.002","url":null,"abstract":"<div><p>The atrophic myocardium resulting from mechanical unloading and nutritional deprivation is considered crucial as maladaptive remodeling directly associated with heart failure, as well as interstitial fibrosis. Conversely, myocardial hypertrophy resulting from hemodynamic loading is perceived as compensatory stress adaptation. We previously reported the abundant presence of highly redox-active polysulfide molecules, termed supersulfide, with two or more sulfur atoms catenated in normal hearts, and the supersulfide catabolism in pathologic hearts after myocardial infarction correlated with worsened prognosis of heart failure. However, the impact of supersulfide on myocardial remodeling remains unclear. Here, we investigated the involvement of supersulfide metabolism in cardiomyocyte remodeling, using a model of adenosine 5′-triphosphate (ATP) receptor-stimulated atrophy and endothelin-1 receptor-stimulated hypertrophy in neonatal rat cardiomyocytes. Results revealed contrasting changes in intracellular supersulfide and its catabolite, hydrogen sulfide (H₂S), between cardiomyocyte atrophy and hypertrophy. Stimulation of cardiomyocytes with ATP decreased supersulfide activity, while H₂S accumulation itself did not affect cardiomyocyte atrophy. This supersulfide catabolism was also involved in myofibroblast formation of neonatal rat cardiac fibroblasts. Thus, unraveling supersulfide metabolism during myocardial remodeling may lead to the development of novel therapeutic strategies to improve heart failure.</p></div>","PeriodicalId":16786,"journal":{"name":"Journal of pharmacological sciences","volume":"155 4","pages":"Pages 121-130"},"PeriodicalIF":3.5,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1347861324000392/pdfft?md5=a62f72057fa85a8a8ca86cdc6437f47b&pid=1-s2.0-S1347861324000392-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141091090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of paeonol against cognitive impairment in lung diseases","authors":"Yen-Chang Chen ,&nbsp;Jia-Hong Chen ,&nbsp;Cheng-Fang Tsai ,&nbsp;Chen-Yun Wu ,&nbsp;Chen-Ni Chang ,&nbsp;Chen-Teng Wu ,&nbsp;Wei-Lan Yeh","doi":"10.1016/j.jphs.2024.04.006","DOIUrl":"https://doi.org/10.1016/j.jphs.2024.04.006","url":null,"abstract":"<div><p>Pulmonary inflammation may lead to neuroinflammation resulting in neurological dysfunction, and it is associated with a variety of acute and chronic lung diseases. Paeonol is a herbal phenolic compound with anti-inflammatory and anti-oxidative properties. The aim of this study is to understand the beneficial effects of paeonol on cognitive impairment, pulmonary inflammation and its underlying mechanisms. Pulmonary inflammation-associated cognitive deficit was observed in TNFα-stimulated mice, and paeonol mitigated the cognitive impairment by reducing the expressions of interleukin (IL)-1β, IL-6, and NOD-like receptor family pyrin domain-containing 3 (NLRP3) in hippocampus. Moreover, elevated plasma miR-34c-5p in lung-inflamed mice was also reduced by paeonol. Pulmonary inflammation induced by intratracheal instillation of TNFα in mice resulted in immune cells infiltration in bronchoalveolar lavage fluid, pulmonary edema, and acute fibrosis, and these inflammatory responses were alleviated by paeonol orally. In MH-S alveolar macrophages, tumor necrosis factor (TNF) α- and phorbol myristate acetate (PMA)-induced inflammasome activation was ameliorated by paeonol. In addition, the expressions of antioxidants were elevated by paeonol, and reactive oxygen species production was reduced. In this study, paeonol demonstrates protective effects against cognitive deficits and pulmonary inflammation by exerting anti-inflammatory and anti-oxidative properties, suggesting a powerful benefit as a potential therapeutic agent.</p></div>","PeriodicalId":16786,"journal":{"name":"Journal of pharmacological sciences","volume":"155 3","pages":"Pages 101-112"},"PeriodicalIF":3.5,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1347861324000367/pdfft?md5=7b307097cfa192b42043d6d54d7040ec&pid=1-s2.0-S1347861324000367-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140880623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfide and polysulfide as pronociceptive mediators: Focus on Cav3.2 function enhancement and TRPA1 activation","authors":"Fumiko Sekiguchi,&nbsp;Maho Tsubota,&nbsp;Atsufumi Kawabata","doi":"10.1016/j.jphs.2024.04.007","DOIUrl":"https://doi.org/10.1016/j.jphs.2024.04.007","url":null,"abstract":"<div><p>Reactive sulfur species including sulfides, polysulfides and cysteine hydropersulfide play extensive roles in health and disease, which involve modification of protein functions through the interaction with metals bound to the proteins, cleavage of cysteine disulfide (S–S) bonds and S-persulfidation of cysteine residues. Sulfides over a wide micromolar concentration range enhance the activity of Ca_v3.2 T-type Ca²⁺ channels by eliminating Zn²⁺ bound to the channels, thereby promoting somatic and visceral pain. Ca_v3.2 is under inhibition by Zn²⁺ in physiological conditions, so that sulfides function to reboot Ca_v3.2 from Zn²⁺ inhibition and increase the excitability of nociceptors. On the other hand, polysulfides generated from sulfides activate TRPA1 channels via cysteine S-persulfidation, thereby facilitating somatic, but not visceral, pain. Thus, Ca_v3.2 function enhancement by sulfides and TRPA1 activation by polysulfides, synergistically accelerate somatic pain signals. The increased activity of the sulfide/Ca_v3.2 system, in particular, appears to have a great impact on pathological pain, and may thus serve as a therapeutic target for treatment of neuropathic and inflammatory pain including visceral pain.</p></div>","PeriodicalId":16786,"journal":{"name":"Journal of pharmacological sciences","volume":"155 3","pages":"Pages 113-120"},"PeriodicalIF":3.5,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1347861324000379/pdfft?md5=1f97a5fce3fdafab5cd2ab4a64c8edd8&pid=1-s2.0-S1347861324000379-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140894485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfur metabolism as a new therapeutic target of heart failure","authors":"Akiyuki Nishimura ,&nbsp;Xiaokang Tang ,&nbsp;Liuchenzi Zhou ,&nbsp;Tomoya Ito ,&nbsp;Yuri Kato ,&nbsp;Motohiro Nishida","doi":"10.1016/j.jphs.2024.04.005","DOIUrl":"https://doi.org/10.1016/j.jphs.2024.04.005","url":null,"abstract":"<div><p>Sulfur-based redox signaling has long attracted attention as critical mechanisms underlying the development of cardiac diseases and resultant heart failure. Especially, post-translational modifications of cysteine (Cys) thiols in proteins mediate oxidative stress-dependent cardiac remodeling including myocardial hypertrophy, senescence, and interstitial fibrosis. However, we recently revealed the existence of Cys persulfides and Cys polysulfides in cells and tissues, which show higher redox activities than Cys and substantially contribute to redox signaling and energy metabolism. We have established simple evaluation methods that can detect polysulfides in proteins and inorganic polysulfides in cells and revealed that polysulfides abundantly expressed in normal hearts are dramatically catabolized by exposure to ischemic/hypoxic and environmental electrophilic stress, which causes vulnerability of the heart to mechanical load. Accumulation of hydrogen sulfide, a nucleophilic catabolite of persulfides/polysulfides, may lead to reductive stress in ischemic hearts, and perturbation of polysulfide catabolism can improve chronic heart failure after myocardial infarction in mice. This review focuses on the (patho)physiological role of sulfur metabolism in hearts, and proposes that sulfur catabolism during ischemic/hypoxic stress has great potential as a new therapeutic strategy for the treatment of ischemic heart failure.</p></div>","PeriodicalId":16786,"journal":{"name":"Journal of pharmacological sciences","volume":"155 3","pages":"Pages 75-83"},"PeriodicalIF":3.5,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1347861324000355/pdfft?md5=8a619fee26fd3f1f2ce79790048591f4&pid=1-s2.0-S1347861324000355-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140647141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of anti-claudin 18.2 monoclonal antibody zolbetuximab alone or combined with chemotherapy or programmed cell death-1 blockade in syngeneic and xenograft gastric cancer models","authors":"Toshihide Nishibata ,&nbsp;Jane Weng ,&nbsp;Keisuke Omori ,&nbsp;Yuji Sato ,&nbsp;Taisuke Nakazawa ,&nbsp;Tomoyuki Suzuki ,&nbsp;Tomohiro Yamada ,&nbsp;Ikumi Nakajo ,&nbsp;Fumitaka Kinugasa ,&nbsp;Özlem Türeci ,&nbsp;Uğur Şahin ,&nbsp;Taku Yoshida","doi":"10.1016/j.jphs.2024.04.004","DOIUrl":"https://doi.org/10.1016/j.jphs.2024.04.004","url":null,"abstract":"<div><p>The development of targeted cancer therapies based on monoclonal antibodies against tumor-associated antigens has progressed markedly over recent decades. This approach is dependent on the identification of tumor-specific, normal tissue-sparing antigenic targets. The transmembrane protein claudin-18 splice variant 2 (CLDN18.2) is frequently and preferentially displayed on the surface of primary gastric adenocarcinomas, making it a promising monoclonal antibody target. Phase 3 studies of zolbetuximab, a chimeric immunoglobulin G1 monoclonal antibody targeting CLDN18.2, combined with 5-fluorouracil/leucovorin plus oxaliplatin (modified FOLFOX6) or capecitabine plus oxaliplatin (CAPOX) in advanced or metastatic first-line gastric or gastroesophageal junction (G/GEJ) adenocarcinoma have demonstrated favorable clinical results with zolbetuximab. In studies using xenograft or syngeneic models with gastric cancer cell lines, zolbetuximab mediated death of CLDN18.2-positive human cancer cell lines via antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity in vitro and demonstrated anti-tumor efficacy as monotherapy and combined with chemotherapy in vivo. Mice treated with zolbetuximab plus chemotherapy displayed a significantly higher frequency of tumor-infiltrating CD8⁺ T cells versus vehicle/isotype control-treated mice. Furthermore, zolbetuximab combined with an anti-mouse programmed cell death-1 antibody more potently inhibited tumor growth compared with either agent alone. These results support the potential of zolbetuximab as a novel treatment option for G/GEJ adenocarcinoma.</p></div>","PeriodicalId":16786,"journal":{"name":"Journal of pharmacological sciences","volume":"155 3","pages":"Pages 84-93"},"PeriodicalIF":3.5,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1347861324000343/pdfft?md5=9aa4b7ac0f325f5b18aeb040a6b66732&pid=1-s2.0-S1347861324000343-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140650611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deficiency of interleukin-19 exacerbates acute lung injury induced by intratracheal treatment of hydrochloric acid","authors":"Kazuhiro Nishiyama ,&nbsp;Joji Horikoshi ,&nbsp;Toko Maehara ,&nbsp;Miyuu Tanaka ,&nbsp;Takashi Tanida ,&nbsp;Koichi Kawada ,&nbsp;Susumu Takeshita ,&nbsp;Naoshige Ono ,&nbsp;Takeshi Izawa ,&nbsp;Mitsuru Kuwamura ,&nbsp;Yasu-Taka Azuma","doi":"10.1016/j.jphs.2024.04.003","DOIUrl":"10.1016/j.jphs.2024.04.003","url":null,"abstract":"<div><p>Interleukin (IL-19) belongs to the IL-10 family of cytokines and plays diverse roles in inflammation, cell development, viral responses, and lipid metabolism. Acute lung injury (ALI) is a severe respiratory condition associated with various diseases, including severe pneumonia, sepsis, and trauma, lacking established treatments. However, the role of IL-19 in acute inflammation of the lungs is unknown. We reported the impact of IL-19 functional deficiency in mice crossed with an ALI model using HCl. Lungs damages, neutrophil infiltration, and pulmonary edema induced by HCl were significantly worse in IL-19 knockout (KO) mice than in wild-type (WT) mice. mRNA expression levels of C-X-C motif chemokine ligand 1 (CXCL1) and IL-6 in the lungs were significantly higher in IL-19 KO mice than in WT mice. Little apoptosis was detected in lung injury in WT mice, whereas apoptosis was observed in exacerbated area of lung injury in IL-19 KO mice. These results are the first to show that IL-19 is involved in acute inflammation of the lungs, suggesting a novel molecular mechanism in acute respiratory failures. If it can be shown that neutrophils have IL-19 receptors and that IL-19 acts directly on them, it would be a novel drug target.</p></div>","PeriodicalId":16786,"journal":{"name":"Journal of pharmacological sciences","volume":"155 3","pages":"Pages 94-100"},"PeriodicalIF":3.5,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1347861324000331/pdfft?md5=1f87f3b3dbcba4297e1f78504d8e39d5&pid=1-s2.0-S1347861324000331-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140787739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Actions of remimazolam on inhibitory transmission of rat spinal dorsal horn neurons","authors":"Rintaro Hoshino ,&nbsp;Nobuko Ohashi ,&nbsp;Daisuke Uta ,&nbsp;Masayuki Ohashi ,&nbsp;Hiroyuki Deguchi ,&nbsp;Hiroshi Baba","doi":"10.1016/j.jphs.2024.04.002","DOIUrl":"https://doi.org/10.1016/j.jphs.2024.04.002","url":null,"abstract":"<div><p>Remimazolam is an ultra-short benzodiazepine that acts on the benzodiazepine site of γ-aminobutyric acid (GABA) receptors in the brain and induces sedation. Although GABA receptors are found localized in the spinal dorsal horn, no previous studies have reported the analgesic effects or investigated the cellular mechanisms of remimazolam on the spinal dorsal horn. Behavioral measures, immunohistochemistry, and <em>in vitro</em> whole-cell patch-clamp recordings of dorsal horn neurons were used to assess synaptic transmission. Intrathecal injection of remimazolam induced behavioral analgesia in inflammatory pain-induced mechanical allodynia (six rats/dose; p &lt; 0.05). Immunohistochemical staining revealed that remimazolam suppressed spinal phosphorylated extracellular signal-regulated kinase activation (five rats/group, p &lt; 0.05). <em>In vitro</em> whole-cell patch-clamp analysis demonstrated that remimazolam increased the frequency of GABAergic miniature inhibitory post-synaptic currents, prolonged the decay time (six rats; p &lt; 0.05), and enhanced GABA currents induced by exogenous GABA (seven rats; p &lt; 0.01). However, remimazolam did not affect miniature excitatory post-synaptic currents or amplitude of monosynaptic excitatory post-synaptic currents evoked by Aδ- and C-fiber stimulation (seven rats; p &gt; 0.05). This study suggests that remimazolam induces analgesia by enhancing GABAergic inhibitory transmission in the spinal dorsal horn, suggesting its potential utility as a spinal analgesic for inflammatory pain.</p></div>","PeriodicalId":16786,"journal":{"name":"Journal of pharmacological sciences","volume":"155 2","pages":"Pages 63-73"},"PeriodicalIF":3.5,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S134786132400032X/pdfft?md5=e9004f36617bcf5850b5b4c08db4c556&pid=1-s2.0-S134786132400032X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140550949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}