Journal of Plant Biochemistry & Physiology最新文献_第8页

Impact of Engineered Si Nanoparticles on Seed Germination, Vigour Index and Genotoxicity Assessment via DNA Damage of Root Tip Cells in Lens culinaris 工程纳米硅对菜蛾种子萌发、活力指数及根尖细胞DNA损伤遗传毒性的影响

Journal of Plant Biochemistry & Physiology Pub Date : 2018-01-01 DOI: 10.4172/2329-9029.1000218

Z. Khan

{"title":"Impact of Engineered Si Nanoparticles on Seed Germination, Vigour Index and Genotoxicity Assessment via DNA Damage of Root Tip Cells in Lens culinaris","authors":"Z. Khan","doi":"10.4172/2329-9029.1000218","DOIUrl":"https://doi.org/10.4172/2329-9029.1000218","url":null,"abstract":"Engineered nanoparticles are of great scientific interest due to their wide variety of potential applications in biomedical, agricultural, optical and electronic fields. The present study has been designed to study the effect of Si nanoparticle (SiNP) on plant growth parameters and to detect the possible genotoxicity induced by them. Seed germination results indicated that SiNP at lower concentration promotes seed germination, Vigour index and biomass; however, at higher concentrations they showed deviated results. To study toxicological end points microscopic examination of root tip cells were carried out. The result showed that exposure to the nanoparticle increase the number of chromosomal aberrations significantly. Dose-dependent decrease in Mitotic index (MI) in the treated populations was observed as compared to control. The result suggests potential of nanoparticle in causing genomic instability by impairing mitosis and altering DNA by inducing chromosomal anomalies. Lower concentration of Si NP can induce positive results on germination and biomass of lentils Si Nanoparticles can penetrate the tissue system through seed or foliar dressing and interfere with the cell division cycle, inducing nanotoxicity.","PeriodicalId":16778,"journal":{"name":"Journal of Plant Biochemistry & Physiology","volume":"21 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75306226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Isopentenyletransferase Gene Enhances Drought Tolerance in Genetically Engineered Sweetpotato (Ipomoea batatas (L.) Lam) 异戊烯基转移酶基因提高转基因甘薯的抗旱性Lam)

Journal of Plant Biochemistry & Physiology Pub Date : 2018-01-01 DOI: 10.4172/2329-9029.1000221

Sylvia Nawiri, Richard O. Oduor, W. Mbinda

引用次数: 6

Photosynthesis and Kaempferol Yields of Soy Leaves Under ABA Application and Mechanical Wounding ABA和机械损伤对大豆叶片光合作用和山奈酚产量的影响

Journal of Plant Biochemistry & Physiology Pub Date : 2018-01-01 DOI: 10.4172/2329-9029.1000215

Ratnayaka Hh, S. Boue, T. Dinh, Letunova Sb, R. Cherubin

{"title":"Photosynthesis and Kaempferol Yields of Soy Leaves Under ABA Application and Mechanical Wounding","authors":"Ratnayaka Hh, S. Boue, T. Dinh, Letunova Sb, R. Cherubin","doi":"10.4172/2329-9029.1000215","DOIUrl":"https://doi.org/10.4172/2329-9029.1000215","url":null,"abstract":"Environmentally sound plant treatments that can impose mild physiological stress and elicit bioaccumulation of useful phytochemicals such as kaempferols are limited. We tested ABA foliar application, 100 or 200 μM, and two types of leaf wounding, piercing or hole punching in young greenhouse-grown soy plants. Leaf gas exchange and A/Ci response, ΦPSII, pigments and antiradical activity were measured using the same leaf and kaempferols were measured in the leaf above. ABA 200 μM-treated plants had ≥ 20% less gas exchange and 17% less ETR, but greater Vcmax and Jmax compared to control. They had 55% and 100% more stomatal limitation to Pnet and ΦPSII, respectively, than control. Leaf-wounded plants showed the lowest stomatal limitation to either Pnet or ΦPSII. Leaf piercing increased chlorophylls 39% and carotenoids 38% compared to control. Six kaempferols quantified were found to be mono-, di- and triglycosides. Each leaf treatment increased total kaempferol yield ranging from 42% in ABA 100 μM to 68% in ABA 200 μM treatment compared to control. In general, kaempferol yields were positively correlated to Pnet in ABA 100 μM-treated plants and to gs in ABA 200 μM-treated plants but negatively correlated to Pnet in leaf-pierced plants. ABA application and wounding affected the association between photosynthetic primary metabolism and kaempferol accumulation differently. Both ABA application and wounding are promising leaf treatments for eliciting kaempferol accumulation in young soy leaves.","PeriodicalId":16778,"journal":{"name":"Journal of Plant Biochemistry & Physiology","volume":"36 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79798641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Gene Expression Profiling in Halwani and Baladi Grapevine (Vitis vinifera L.) Cultivars under Saline Conditions Halwani和Baladi葡萄基因表达谱分析盐渍条件下的栽培品种

Journal of Plant Biochemistry & Physiology Pub Date : 2018-01-01 DOI: 10.4172/2329-9029.1000222

B. Saleh, E. Alshehada

引用次数: 5

In Vitro Plant Regeneration of Sweetpotato Through Direct Shoot Organogenesis 甘薯直接茎器官发生离体植株再生的研究

Journal of Plant Biochemistry & Physiology Pub Date : 2018-01-01 DOI: 10.4172/2329-9029.1000207

Nzaro G Makenzi, W. Mbinda, R. O. Okoth, M. Ngugi

{"title":"In Vitro Plant Regeneration of Sweetpotato Through Direct Shoot Organogenesis","authors":"Nzaro G Makenzi, W. Mbinda, R. O. Okoth, M. Ngugi","doi":"10.4172/2329-9029.1000207","DOIUrl":"https://doi.org/10.4172/2329-9029.1000207","url":null,"abstract":"Sweetpotato (Ipomoea batata) is an important, nutritionally rich vegetable crop, but severely affected by environmental stresses, pests and diseases which cause massive yield and quality losses. Genetic manipulation is becoming an important method for sweetpotato improvement. In the present study, a reproducible and highly efficient protocol for in vitro plant regeneration of six Kenyan farmer preferred sweetpotato, Enaironi, KEMB 36, KSP36, Mugande, Kalamb nyerere, SPK 013 and SPK004 through direct shoot organogenesis from stem internodes explants was developed. The results revealed that Kalamb nyerere had the highest number of adventitious bud; for light (5.33 and 4.33) and dark (8.00 and 5.00) induction condition for all TDZ hormone level (0.25 mg/l and 0.15 mg/l). When explants incubated in 0.10 mg/l NAA the regeneration frequencies were the highest at 83.33% (Jewel) and 96.67% (Kalamb nyerere) for adventitious buds recovered from light and darkness respectively. This was the optimal auxin concentration which gave the maximum regeneration frequency with adventitious buds recovered from the dark. The best Kenyan sweetpotato genotypes for direct shoot organogenesis were Kalamb nyerere, Kemb 36 and SPK 004. The protocol presented in this work is suitable for improvement of sweetpotato genotypes through tissue culture methods and or genetic transformation.","PeriodicalId":16778,"journal":{"name":"Journal of Plant Biochemistry & Physiology","volume":"82 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85814926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Investigation of the Biologically Active Substances Obtained from Johrenia paucijuga (DC) of the Species Bornm 产自本属白刺藤的生物活性物质的研究

Journal of Plant Biochemistry & Physiology Pub Date : 2018-01-01 DOI: 10.4172/2329-9029.1000208

M. Hg, Serkerov Sv, Alasgarova An

引用次数: 0

Structure and Molecular Characterization of Diadenosine Polyphosphate Hydrolase in Brachypodium distachyon 短茅二叉藻二腺苷多磷酸水解酶的结构与分子表征

Journal of Plant Biochemistry & Physiology Pub Date : 2018-01-01 DOI: 10.4172/2329-9029.1000220

Motohiro Tanaka, Igor Iamshchikov, Y. Kato, R. Sabirov, O. Gusev, W. Sakamoto, M. Sugimoto

{"title":"Structure and Molecular Characterization of Diadenosine Polyphosphate Hydrolase in Brachypodium distachyon","authors":"Motohiro Tanaka, Igor Iamshchikov, Y. Kato, R. Sabirov, O. Gusev, W. Sakamoto, M. Sugimoto","doi":"10.4172/2329-9029.1000220","DOIUrl":"https://doi.org/10.4172/2329-9029.1000220","url":null,"abstract":"Putative diadenosine polyphosphate (ApnA) hydrolase gene, which encodes an amino acid sequence showing homology with that of Arabidopsis long-chain (ApnA) (n=5–6) hydrolase (AtNUDX13) and which conserves nudix motif and glycine tripeptide motif, was identified from Brachypodium distachyon. The mature form of Brachypodium (ApnA) hydrolase (BraNUDX15) catalyzed long-chain (ApnA) and Ap4A, Ap4G, Gp4G, and dCTP, showing different substrate specificity from Arabidopsis (ApnA) hydrolases AtNUDX13, 25, 26, and 27. BraNUDX15 required Mg2+ and produced ATP from (ApnA), indicating asymmetrical (ApnA) hydrolase as the same as Arabidopsis (ApnA) hydrolases. Results show that BraNUDX15 gene was up-regulated by UV-A, UV-B, and UV-C irradiation and downregulated by drought stress, but it was left unchanged by salt stress. Subcellular localization indicated that the BraNUDX15 protein was colocalized with the surface of the chloroplasts. These results suggest BraNUDX15 as a unique (ApnA) hydrolase with different substrate specificity from those of Arabidopsis (ApnA) hydrolases. It might play a role in regulating (ApnA) levels in chloroplasts under conditions of drought stress and UV irradiation.","PeriodicalId":16778,"journal":{"name":"Journal of Plant Biochemistry & Physiology","volume":"22 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91230061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

An Efficient Throughput Protocol for Validation of Pathotyping by Enzymatic Mismatch Cleavage 酶错配切割病理分型验证的高效通量方案

Journal of Plant Biochemistry & Physiology Pub Date : 2018-01-01 DOI: 10.4172/2329-9029.1000203

M. Jawhar, B. Till, A. Albaterni, A. Skiheita, M. Arabi, N. Mirali

{"title":"An Efficient Throughput Protocol for Validation of Pathotyping by Enzymatic Mismatch Cleavage","authors":"M. Jawhar, B. Till, A. Albaterni, A. Skiheita, M. Arabi, N. Mirali","doi":"10.4172/2329-9029.1000203","DOIUrl":"https://doi.org/10.4172/2329-9029.1000203","url":null,"abstract":"Early diagnosis of fungal pathotypes is one of the most important aspects for plant breeders. It provides a rapid means for selecting the correct mutant lines to ensure durability of disease resistance and also for proper field management practices. Traditional diagnostic tools for pathogens have been based on targeted cultures, PCRbased approaches, and/or phenotypic evaluation of disease response in specific plant genotypes. These methods detect only known pathogenic agents, can introduce bias, and can fail to recognize novel variants or races due to their narrow scope. While enzymatic mismatch cleavage has been described for many plant and animal species, the validate that technique for haploid microorganisms is also needed. In this work, optimized low cost method for pathotyping Fusarium oxysporum f. sp. cubense (Foc), the causal agent of fusarium wilt of banana (Musa spp.), was established using self-extracted nuclease and agarose gel electrophoresis. Gene-specific primers were designed from the whole fungal genome for use in the enzymatic mismatch cleavage on Foc representatives of the major races of major banana producing countries. Gene-specific primer pairs were used to optimize enzymatic mismatch cleavage and polymorphism discovery in two SNF1 and FOW2 genes. The protocol is rapid, inexpensive and can robustly distinguish pathotypes in Foc strains, without high informatics load of DNA sequencing.","PeriodicalId":16778,"journal":{"name":"Journal of Plant Biochemistry & Physiology","volume":"8 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88605734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Responses of Primary Metabolites and Glucosinolates in Sulfur Deficient-Cabbage (Brassica rapa L. ssp. Pekinensis) 缺硫甘蓝初级代谢物和硫代葡萄糖苷的响应。学报)

Journal of Plant Biochemistry & Physiology Pub Date : 2018-01-01 DOI: 10.4172/2329-9029.1000223

J. Sung, Seung-A Baek, Jae-Kwang Kim, Yangmin X. Kim, Yejin Lee, Seul-Bi Lee, Deog-Bae Lee, Harin Jung

{"title":"Responses of Primary Metabolites and Glucosinolates in Sulfur Deficient-Cabbage (Brassica rapa L. ssp. Pekinensis)","authors":"J. Sung, Seung-A Baek, Jae-Kwang Kim, Yangmin X. Kim, Yejin Lee, Seul-Bi Lee, Deog-Bae Lee, Harin Jung","doi":"10.4172/2329-9029.1000223","DOIUrl":"https://doi.org/10.4172/2329-9029.1000223","url":null,"abstract":"Sulfur (S) is an essential mineral nutrient for plant growth and development and is a key component of many biological compounds. As S acquisition and assimilation have important roles in plant metabolism, S-deficient responses are closely involved in different plant constituents. In this study, we examined the effects of S deficiency on primary metabolism and glucosinolate (GSL) content in cabbage (Brassica rapa) plants. Soluble sugars such as glucose, fructose, galactose, and xylose, were up to 0.19-fold lower under S deficiency, and these changes were more pronounced with long-term (15 d) S deficiency. Significant increases in amino acids were observed in terms of glutamine (6.35-fold), glycine (20.54), serine (3.56), threonine (3.25), phenylalanine (4.07), β-alanine (7.88), and proline (4.58). S deficiency led to large accumulation of an indolyl GSL, 4-methoxyglucobrassicin, in both shoots (2.68 fold) and roots (5.99 fold). GSLs were positively correlated with the majority of primary metabolites in the shoots, but negative in the roots. Thus, at least in cabbage plants, the interplay between primary metabolism and GSLs appeared to be tissue-dependent, and the metabolic interaction between both metabolites should be elucidated.","PeriodicalId":16778,"journal":{"name":"Journal of Plant Biochemistry & Physiology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86347660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Rice Salt-Tolerance Enhancement by Expression of 1-Aminocyclopropane-1- Carboxylic Acid Oxidase Gene from Salt Tolerant Barley 从耐盐大麦中表达1-氨基环丙烷-1-羧酸氧化酶基因增强水稻耐盐性

Journal of Plant Biochemistry & Physiology Pub Date : 2018-01-01 DOI: 10.4172/2329-9029.1000226

M. Sugimoto, Y. Houjyo, M. Maekawa, R. Terada

引用次数: 1