Journal of pediatric surgery最新文献

{"title":"Invited Commentary on: Defining an Anastomotic Stricture Following Oesophageal Atresia Repair","authors":"Simon Eaton , Natalie Durkin , Paolo De Coppi","doi":"10.1016/j.jpedsurg.2025.162468","DOIUrl":"10.1016/j.jpedsurg.2025.162468","url":null,"abstract":"","PeriodicalId":16733,"journal":{"name":"Journal of pediatric surgery","volume":"60 9","pages":"Article 162468"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Storage of Placental Blood – A Feasibility Study for Development of a Fetal Blood Bank","authors":"Keyonna M. Williams ,&nbsp;Caitlin Clifford ,&nbsp;Danielle Dougherty ,&nbsp;Connor Plagens ,&nbsp;Scott La Dell ,&nbsp;Alissa R. Carver ,&nbsp;Shih-Hon Li ,&nbsp;Erin E. Perrone","doi":"10.1016/j.jpedsurg.2025.162459","DOIUrl":"10.1016/j.jpedsurg.2025.162459","url":null,"abstract":"<div><h3>Objective</h3><div>Neonates transfused with adult donor blood (rich in HbA) have a precipitous drop in fetal hemoglobin (HbF) levels. This study evaluates the feasibility of collection and storage of placental cord blood.</div></div><div><h3>Methods</h3><div>Prospective study patients with uncomplicated term pregnancies undergoing scheduled cesarean section were recruited from July 2022–December 2023. Cord blood was collected after delivery for processing and storage in our institutional blood bank. Samples were analyzed at 0, 7, 14, and 21 days.</div></div><div><h3>Results</h3><div>Thirty patients were recruited (55 % Caucasian with median maternal age 34 years, gestational age 39 weeks, birth weight 3.48 kg). Median collection volume was 83 mL; 8 collections were excluded due to inadequate volume. The median hemolysis was 0.10 % on Day 0 and 0.23 % on Day 21 with median loss 2.5 %. Median biochemical storage lesion at 21 days was within expectations (24.5 mmol/L potassium, 71.2 mg/dL free hemoglobin, pH 6.38, LDH 191 IU/L). All day 21 blood cultures were negative. Hemoglobin fractions remained unchanged between 0 and 21 days (16.2–17.8 % HbA and 83.8–82.2 % HbF).</div></div><div><h3>Conclusion</h3><div>Placental cord blood can be safely collected, processed, and stored for 21 days as a potential autologous blood product for neonates.</div></div><div><h3>Type of study</h3><div>Level II, Prospective Cohort.</div></div>","PeriodicalId":16733,"journal":{"name":"Journal of pediatric surgery","volume":"60 9","pages":"Article 162459"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on: Aspartate Aminotransferase-to-Platelet Ratio Index (APRi) as Biomarker for Liver Damage in Biliary Atresia (BA): A Meta-Analysis","authors":"Elizabeth Brits,&nbsp;Stephen Brown,&nbsp;Lezelle Botes,&nbsp;Joseph B. Sempa,&nbsp;Michael Pienaar","doi":"10.1016/j.jpedsurg.2025.162401","DOIUrl":"10.1016/j.jpedsurg.2025.162401","url":null,"abstract":"","PeriodicalId":16733,"journal":{"name":"Journal of pediatric surgery","volume":"60 9","pages":"Article 162401"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invited Commentary on: Screening Practices for Müllerian Anomalies in Patients With Known Renal or Urologic Anomalies: A Retrospective Chart Review","authors":"Jennifer E. Dietrich","doi":"10.1016/j.jpedsurg.2025.162394","DOIUrl":"10.1016/j.jpedsurg.2025.162394","url":null,"abstract":"","PeriodicalId":16733,"journal":{"name":"Journal of pediatric surgery","volume":"60 9","pages":"Article 162394"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Report on Testicular Tissue Cryopreservation for Fertility Preservation in Children: Confirmation of Germ Cell Preservation and Few Surgical Complications","authors":"Christopher J. McCauley ,&nbsp;Kathryn L. McElhinney ,&nbsp;Barbara A. Lockart ,&nbsp;Lori Schmitt ,&nbsp;Amanda Zielen ,&nbsp;Kyle E. Orwig ,&nbsp;Miguel Reyes-Múgica ,&nbsp;Emilie K. Johnson ,&nbsp;Timothy B. Lautz ,&nbsp;Monica M. Laronda ,&nbsp;Erin E. Rowell","doi":"10.1016/j.jpedsurg.2025.162390","DOIUrl":"10.1016/j.jpedsurg.2025.162390","url":null,"abstract":"<div><h3>Background</h3><div>Testicular tissue cryopreservation<span> (TTC) is an experimental method for fertility preservation<span> in pediatric patients facing a fertility threatening treatment regimen or diagnosis. TTC requires a surgical procedure for the removal of testicular tissue, advanced tissue processing capabilities, and long-term tissue storage at a cryopreservation facility. The goal of this work is to evaluate the surgical and pathologic outcomes of patients enrolled in an ongoing clinical protocol for fertility preservation in children.</span></span></div></div><div><h3>Methods</h3><div><span>Patients were prospectively enrolled in an IRB-approved protocol from 2015 to 2023 at a single institution. Children were eligible for enrollment if they possessed a fertility threatening diagnosis or required treatment that placed them at high level of increased risk for prolonged azoospermia. In this work we report </span>patient characteristics, surgical outcomes, and pathologic findings of our cohort.</div></div><div><h3>Results</h3><div><span>TTC was performed for 144 patients. The median age of patients was 8.89 years (Range: 0.3–24.5 years). Most patients were prepubertal (n = 126, 87.5 %) and carried a diagnosis of malignancy (n = 117, 81.2 %). Overall, 45.8 % of patients received pre-operative chemotherapy. There was a low rate of TTC-related </span>surgical complications (n = 3, 2.1 %). Germ cells were present in 97.9 % (141/144) of samples. Malignancy was discovered in testicular tissue in 1.4 % (n = 2) of patients. Of the initially enrolled cohort, 89.4 % (n = 129) are living.</div></div><div><h3>Conclusions</h3><div>Testicular tissue cryopreservation is a safe and effective procedure for fertility preservation in the pediatric population. Germ cells were present in nearly all preserved tissue, providing the possibility of future fertility restoration in these children pending further scientific advancement.</div></div><div><h3>Level of evidence</h3><div>Level III – Prospective cohort study.</div></div>","PeriodicalId":16733,"journal":{"name":"Journal of pediatric surgery","volume":"60 9","pages":"Article 162390"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening Practices for Müllerian Anomalies in Patients With Known Renal or Urologic Anomalies: A Retrospective Chart Review","authors":"Sofie Rosenberg ,&nbsp;Kendra Hutchens ,&nbsp;Lauryn P. Roth ,&nbsp;Laurel Beaty ,&nbsp;Vijaya M. Vemulakonda ,&nbsp;Veronica I. Alaniz","doi":"10.1016/j.jpedsurg.2025.162393","DOIUrl":"10.1016/j.jpedsurg.2025.162393","url":null,"abstract":"<div><h3>Introduction/background</h3><div>Approximately 20–40 % of females diagnosed with renal anomalies<span> will have a coexistent Müllerian anomaly. Müllerian anomalies can have significant implications on reproductive health, however, no formal guidelines exist to direct screening practices for patients within this high-risk population.</span></div></div><div><h3>Objective</h3><div>This study aims to establish a baseline incidence and description of pelvic imaging practices among female patients with renal anomalies at a tertiary children's hospital.</div></div><div><h3>Methods</h3><div>Retrospective chart review of female patients aged 0–25 years with congenital renal anomalies who presented to a tertiary children's hospital between January 2015 and June 2022.</div></div><div><h3>Results</h3><div><span>A total of 212 patients met inclusion criteria. The most common congenital renal anomaly diagnoses included multicystic kidney disease (61, 28.8 %), </span>renal agenesis<span> (39, 18.4 %), and renal dysplasia/hypoplasia (36, 17.0 %). The average age of renal anomaly diagnosis was 8.6 years (SD = 5.8, range 0–20). Within this patient population, 125 (59.0 %) received at least one pelvic imaging study and 75 (35.4 %) reported an evaluation of reproductive structures. The most common imaging modality that evaluated reproductive structures was pelvic ultrasound (59/75, 78.7 %), which occurred on average at age 13.7 (SD 4.7). Uterine or vaginal anomalies were identified in 22/75 (29.3 %) patients who had their reproductive anatomy evaluated on imaging. Only one imaging study was ordered for the indication of Müllerian anomaly screening.</span></div></div><div><h3>Discussion</h3><div>Most female patients with renal anomalies do not receive imaging that evaluates reproductive anatomy. When imaging does occur, it is ordered for indications other than screening for Müllerian anomalies. Screening protocols need to be developed for early detection of Müllerian anomalies in this high-risk population.</div></div><div><h3>Conclusions</h3><div>Findings demonstrate that female patients with renal anomalies are not consistently screened for Müllerian anomalies despite the known association between these anomalies. Lack of guidelines at our tertiary institution may delay diagnosis and treatment of these conditions.</div></div><div><h3>Study type</h3><div>This study is a level 2 retrospective chart review.</div></div>","PeriodicalId":16733,"journal":{"name":"Journal of pediatric surgery","volume":"60 9","pages":"Article 162393"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on: Bringing Diagnostic Accuracy Back Into Focus: The Case of APRi in Biliary Atresia","authors":"Javier Arredondo Montero","doi":"10.1016/j.jpedsurg.2025.162402","DOIUrl":"10.1016/j.jpedsurg.2025.162402","url":null,"abstract":"","PeriodicalId":16733,"journal":{"name":"Journal of pediatric surgery","volume":"60 9","pages":"Article 162402"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invited Commentary on: Williams, et al.: Storage of Placental Blood – A Feasibility Study for Development of a Fetal Blood Bank","authors":"Caitlin R. Eason,&nbsp;S. Christopher Derderian","doi":"10.1016/j.jpedsurg.2025.162467","DOIUrl":"10.1016/j.jpedsurg.2025.162467","url":null,"abstract":"<div><div>The use of cord blood for hematologic disorders is well established, however the concept of utilizing such samples for allogenic blood transfusions in neonates is relatively new. Williams et al. demonstrate the feasibility of establishing a fetal blood bank with detailed protocols for collecting and storing umbilical cord blood. A highlight of their study is the conservation of the proportion of fetal hemoglobin, potentially reducing the risks involved with adult hemoglobin transfusions.</div></div>","PeriodicalId":16733,"journal":{"name":"Journal of pediatric surgery","volume":"60 9","pages":"Article 162467"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on: \"Management of simple appendicitis in children: Early appendectomy vs. non-operative management with subsequent appendectomy for failure: Does initial approach impact surgical complications?\"","authors":"Parth Aphale, Shashank Dokania, Himanshu Shekhar","doi":"10.1016/j.jpedsurg.2025.162620","DOIUrl":"10.1016/j.jpedsurg.2025.162620","url":null,"abstract":"","PeriodicalId":16733,"journal":{"name":"Journal of pediatric surgery","volume":" ","pages":"162620"},"PeriodicalIF":2.5,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144958285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microenvironmental TFPI2 in Hirschsprung disease: Mechanisms in ENCC development","authors":"Dong Sun ,&nbsp;Xintao Zhang ,&nbsp;Xue Ren ,&nbsp;Yu Zhou ,&nbsp;Qiongqian Xu ,&nbsp;Jichang Han ,&nbsp;Chuncan Ma ,&nbsp;Weizhen Wang ,&nbsp;Xiaoyang Liu ,&nbsp;Jiamin Li ,&nbsp;Jian Wang ,&nbsp;Qiangye Zhang ,&nbsp;Dongming Wang ,&nbsp;Weijing Mu ,&nbsp;Peimin Hou ,&nbsp;Aiwu Li","doi":"10.1016/j.jpedsurg.2025.162625","DOIUrl":"10.1016/j.jpedsurg.2025.162625","url":null,"abstract":"<div><h3>Background</h3><div>Hirschsprung disease (HSCR) is a relatively common disorder in pediatric surgery, characterized by the absence of enteric ganglion cells due to defective colonization of enteric neural crest cells (ENCCs) in the intestine. This study aims to investigate alterations in the intestinal microenvironment, focusing on the tissue factor pathway inhibitor 2 (TFPI2) as a potential pathogenic factor.</div></div><div><h3>Methods</h3><div>Transcriptomic datasets from HSCR patients and fetal mouse intestines were analyzed to identify candidate genes. TFPI2 expression was validated by qRT-PCR, immunohistochemistry, and Western blotting. Recombinant TFPI2 protein was applied to ENCC cultures to evaluate its effects on proliferation, apoptosis, and migration, using CCK-8, EdU, flow cytometry, Western blotting, and real-time cell observation. KEGG and GSEA analyses of transcriptomic data were conducted to explore potential mechanisms, and functional rescue was assessed using the PI3K-AKT pathway agonist IGF-1.</div></div><div><h3>Results</h3><div>TFPI2 was identified as a novel microenvironmental factor associated with HSCR. Its expression was significantly elevated in the aganglionic segments of HSCR patients and in the intestines of E14.5 fetal mice. TFPI2 inhibited ENCC proliferation and migration while promoting apoptosis, likely via downregulation of the PI3K-AKT signaling pathway. IGF-1 partially rescued TFPI2-induced effects on proliferation, migration, and apoptosis.</div></div><div><h3>Conclusion</h3><div>This study identifies TFPI2 as a novel microenvironmental factor contributing to ENCC dysfunction in HSCR by inhibiting the PI3K-AKT signaling pathway. These findings provide new insights into the pathogenesis of HSCR and suggest that TFPI2 could be a potential therapeutic target.</div></div>","PeriodicalId":16733,"journal":{"name":"Journal of pediatric surgery","volume":"60 11","pages":"Article 162625"},"PeriodicalIF":2.5,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144958314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}