JOURNAL OF CONTROL VACCINE AND BIOLOGICALS最新文献_第3页

Some complications of the radiofrequency ablation method in patients with non-small cell lung cancer at Nghe An Oncology Hospital 义安肿瘤医院非小细胞肺癌射频消融术的一些并发症

JOURNAL OF CONTROL VACCINE AND BIOLOGICALS Pub Date : 2022-10-31 DOI: 10.56086/jcvb.v2i3.72

Pham Vinh Hung, V. T. Huong, Nguyen Thi Mai Huong

引用次数: 0

STABILITY ASSESSMENT OF THE FIRST NATIONAL STANDARD FOR ROTAVIRUS VACCINE 轮状病毒疫苗首个国家标准稳定性评价

JOURNAL OF CONTROL VACCINE AND BIOLOGICALS Pub Date : 2022-10-31 DOI: 10.56086/jcvb.v2i3.67

N. T. Kiều, Doan Huu Thien, Le Thi Hai Yen, Nguyễn Thị Thu Hương, Nguyen Thi Tuyet Hoa, Do Thi Phuong

{"title":"STABILITY ASSESSMENT OF THE FIRST NATIONAL STANDARD FOR ROTAVIRUS VACCINE","authors":"N. T. Kiều, Doan Huu Thien, Le Thi Hai Yen, Nguyễn Thị Thu Hương, Nguyen Thi Tuyet Hoa, Do Thi Phuong","doi":"10.56086/jcvb.v2i3.67","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.67","url":null,"abstract":"In early 2019, the National Institute for Control of Vaccines and Biologicals (NICVB) implemented a national-level project to establish the first national standard for rotavirus vaccines to be used in the quality control of rotavirus vaccines, code MCQG.Ro.01. However, a stability assessment of the national standard has not been carried out yet. NICVB had performed a study entitled \"Stability assessment of the first national standard for rotavirus vaccine\" with the purpose of providing the prediction shelf life through a thermally accelerated degradation study. In this study, the first national standard for rotavirus vaccine was stored at 4 °C, 25 °C, and 37 °C. At each temperature, the potency of the vaccine was evaluated after 3 days, 5 days, 7 days and 10 days. The stability data were analysed by JMP Pro13 software, the software used to predict the expiry date of vaccines and biological products based on the Arrhenius equation. As a result, the first national standard for rotavirus vaccine, code MCQG.Ro.01, has a predicted shelf life of 27 years when stored at -70 °C.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124902220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating cell block and cell smear technique in the body-cavity fluids cytology technique at Viet Tiep Hospital 越铁医院体腔液细胞学技术中细胞阻滞和细胞涂片技术的评价

JOURNAL OF CONTROL VACCINE AND BIOLOGICALS Pub Date : 2022-10-31 DOI: 10.56086/jcvb.v2i3.70

N. T. Huong, Nguyen Thi Trinh, P. V. Hung, Nguyen Thi Tu Linh

引用次数: 0

DETERMINATE ACCURACY AND LINEARITY OF BSA TEST IN VACCINE BY ELISA elisa法测定疫苗中bsa检测的准确性和线性度

JOURNAL OF CONTROL VACCINE AND BIOLOGICALS Pub Date : 2022-10-31 DOI: 10.56086/jcvb.v2i3.64

Luu Thi Dung, N. T. Ly, Nguyen Thi Hong Dinh, P. Hung, N. Tung, Doan Huu Thien

{"title":"DETERMINATE ACCURACY AND LINEARITY OF BSA TEST IN VACCINE BY ELISA","authors":"Luu Thi Dung, N. T. Ly, Nguyen Thi Hong Dinh, P. Hung, N. Tung, Doan Huu Thien","doi":"10.56086/jcvb.v2i3.64","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.64","url":null,"abstract":"Based on Bovine serum albumin (BSA) is commonly used as a culture medium of vaccine production. According to the World Health Organization, residual BSA with high concentrations in vaccines has an adverse effect on human health. Therefore, the research team evaluated the technical parameters including accuracy, accuracy and linearity of the procedure for determining BSA content by ELISA method in vaccines. \u0000The residual BSA content in the experimental vaccine (Varivax) was carried out according to the procedure of the BSA diagnostic kit, China by the Elisa method. The sample vaccine is diluted in 3 dilutions: 1/20, 1/30, 1/40 so that the OD value of the sample vaccine is within the range of OD values at BSA calibration curve concentrations from 2, 5ng/ml - 40 ng/ml. \u0000Results of study showed that the process specifications were determined including accuracy (t-table (- 2.29 - 2.39) < tα = 2.571), precision (%CV=0.79-1, 21%), linearity (R2 = 0.995, %CV = 0.28 - 4.47%, Δi=-2.47 - 10.95%). \u0000We conclussion that the process of determining BSA content by the Elisa method was evaluated as satisfactory with the specifications of accuracy, precision and linearity.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128629403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EVALUATION OF THE STABILITY OF RNA SARS-CoV-2 IN MULTIPLE FREZEE-THAW CYCLES USING REAL-TIME PCR METHOD 利用实时PCR法评价RNA SARS-CoV-2在多次冻融循环中的稳定性

JOURNAL OF CONTROL VACCINE AND BIOLOGICALS Pub Date : 2022-10-31 DOI: 10.56086/jcvb.v2i3.62

P. Thuy, V. T. Hương, Phạm Thị Minh, Pham Trung Duc, Phung Dang Thi Ngoc

{"title":"EVALUATION OF THE STABILITY OF RNA SARS-CoV-2 IN MULTIPLE FREZEE-THAW CYCLES USING REAL-TIME PCR METHOD","authors":"P. Thuy, V. T. Hương, Phạm Thị Minh, Pham Trung Duc, Phung Dang Thi Ngoc","doi":"10.56086/jcvb.v2i3.62","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.62","url":null,"abstract":"Introduction/ Objectives: According to WHO, clinical samples collected in retrospective clinical studies are required to provide stability claims for each storage condition, such as duration at different temperatures and freeze/thaw cycles[1]. This study was conducted to evaluate the effects of multiple freeze/thaw (FT) cycles on the concentration of RNA SARS-CoV-2 (extracted from retrospective clinical sample) by using Real-time PCR method. \u0000Methods: Descriptive laboratory study. We used a commercial Real-time PCR assay and reference standard for quantification of RNA concentration after each FT cycle. \u0000Results: We observed that the extracted RNA SARS-CoV-2 remained stable and did not show significantly different Ct values (equivalent to viral loads) over 10 FT cycles (p >0.05), compared to those Ct values at time point 0. Variations of Ct values over 10 FT cycles ranged from 0.64% to 1.64%. The mean difference in viral load was 0,12log10IU/ml. \u0000Conclusions: Our study results proved that RNA SARS-CoV-2 extracted from clinical samples can remain stable over 10 freeze-thaw cycles and can be used as a evaluation panel for quality control of in vitro diagnostic test kits detecting SARS-CoV-2.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129751344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Realtime RT-PCR assay for the detection of E gene of SARS-CoV-2 实时RT-PCR检测SARS-CoV-2 E基因的应用

JOURNAL OF CONTROL VACCINE AND BIOLOGICALS Pub Date : 2022-10-31 DOI: 10.56086/jcvb.v2i3.68

Dinh Thi Phuong Thao, V. T. Hương, P. Thuy

引用次数: 0

Comparing cell block diagnostic results with histopathological results and some factors affecting the quality of cell blocks in the diagnosis of body cavity fluid cells at Viet Tiep Hospital 越铁医院体腔液细胞诊断中细胞块诊断结果与组织病理学结果的比较及影响细胞块质量的因素

JOURNAL OF CONTROL VACCINE AND BIOLOGICALS Pub Date : 2022-10-31 DOI: 10.56086/jcvb.v2i3.71

Nguyen Thi Trinh, N. T. Huong, Tran Hong Tram, N. T. Kiều

{"title":"Comparing cell block diagnostic results with histopathological results and some factors affecting the quality of cell blocks in the diagnosis of body cavity fluid cells at Viet Tiep Hospital","authors":"Nguyen Thi Trinh, N. T. Huong, Tran Hong Tram, N. T. Kiều","doi":"10.56086/jcvb.v2i3.71","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.71","url":null,"abstract":"Background/Purpose: To contrast the results of cell block diagnosis with histopathological results and describe some factors that affect the quality of cell blocks. \u0000Methods: The cross-sectional descriptive study was conducted based on the anatomical results of 106 patients of Viet Tiep Hospital \u0000Results: The cell block technique had a sensitivity of 84.4% significantly higher than the sensitivity of the cell smear of 57.8%. The specificity of both tests is above 80%. Cellblock test results were less accurate in yellow fluid samples (13.3%). Blood fluid and pink fluid samples both have over 90% accuracy. The difference was statistically significant with p<0.05. Cellblock test results were less accurate in samples with a sample count of ≤150 ml (52.9%). Samples with a greater number of specimens were over 80% accurate. The difference was statistically significant with p<0.05. There was no difference in the accuracy of cell block test results between specimens at different sites, cancer types, cancer origins, age groups, and sex of the subjects studied. \u0000Conclusion: The cell block technique improves the number of malignant diagnoses compared to histopathology, and has higher sensitivity and lower specificity than the cell smear technique. Factors related to the accuracy of cell block test results include the color of the test fluid and the number of fluids. There was no difference in the accuracy of CB test results on different specimens, different cancer types, different cancer origins, different ages, and genders.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121481527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VALIDATION OF POTENCY TEST OF NEW GENERATION JAPANESE ENCEPHALITIS VACCINE IMOJEV BY PLAQUE FORMING UNIT (PFU) METHOD 新一代乙型脑炎伊莫耶夫疫苗效价检验的菌斑形成单位(pfu)法验证

JOURNAL OF CONTROL VACCINE AND BIOLOGICALS Pub Date : 2022-10-31 DOI: 10.56086/jcvb.v2i3.66

Nguyen Viet Anh, D. H. Anh, N. T. Ha, T. Phuong, N. T. Ly

{"title":"VALIDATION OF POTENCY TEST OF NEW GENERATION JAPANESE ENCEPHALITIS VACCINE IMOJEV BY PLAQUE FORMING UNIT (PFU) METHOD","authors":"Nguyen Viet Anh, D. H. Anh, N. T. Ha, T. Phuong, N. T. Ly","doi":"10.56086/jcvb.v2i3.66","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.66","url":null,"abstract":"Implementation of potency test for new generation Japanese Encephalitis vaccine (IMOJEV) is one of the functions of National Institute for Vaccines and Biologicals (NICVB) prior to market release. Potency test of IMOJEV was carried out by using Plaque Forming Unit (PFU) method for both standard and testing samples, the results was in the approved range of manufacturer. The process of validation of potency test for Japanese Encephalitis vaccine IMOJEV was built and implemented by practical validation method with following parameters: accuracy, repeatability, and intermediate precision of the potency method for Japanese encephalitis vaccine \u0000Results of study showed that the parameters of accuracy, repeatability and intermediate precision of potency test for Japanese Encephalitis IMOJEV were all qualified and in the approved range of manufacturer: titer of standard sample was about 4.25-5.09 log PFU/0.5 ml and titer of IMOJEV was between 4.0-5.8 log PFU/0.5ml \u0000Based on our study conclussion the the potency test for Japanese Encephalitis IMOJEV after validation was qualified for accuracy, repeatability and intermediate precision with coefficient of variation CV ≤ 25% so it is suitable with laboratory condition of NICVB.","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131251920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EXPLORING THE EFFECT OF SLEEP DEPRIVATION AND SOCIAL LIFE ON AGGRESSIVE BEHAVIOR AMONG ADOLESCENTS 探讨睡眠剥夺和社交生活对青少年攻击行为的影响

JOURNAL OF CONTROL VACCINE AND BIOLOGICALS Pub Date : 2022-10-31 DOI: 10.56086/jcvb.v2i3.69

Nguyen Duy Thai, Tran Hong Tram, P. Hung, Doan Huu Thien

引用次数: 0

A STUDY TO INVESTIGATE THE ANTIBACTERIAL EFFECTS, POLYPHENOL CONTENTS AND ANTIOXIDANT ACTIVITIES OF EXTRACTS FROM ANDROGRAPHIS PANICULATA MEDICINAL PLANTS 研究穿心莲药用植物提取物的抑菌作用、多酚含量及抗氧化活性

JOURNAL OF CONTROL VACCINE AND BIOLOGICALS Pub Date : 2022-10-10 DOI: 10.56086/jcvb.v2i3.65

T. Hong, P. Huế, N. T. T. Ha, Nguyễn Thanh Hải

{"title":"A STUDY TO INVESTIGATE THE ANTIBACTERIAL EFFECTS, POLYPHENOL CONTENTS AND ANTIOXIDANT ACTIVITIES OF EXTRACTS FROM ANDROGRAPHIS PANICULATA MEDICINAL PLANTS","authors":"T. Hong, P. Huế, N. T. T. Ha, Nguyễn Thanh Hải","doi":"10.56086/jcvb.v2i3.65","DOIUrl":"https://doi.org/10.56086/jcvb.v2i3.65","url":null,"abstract":"Andrographis paniculata is a traditional medicinal plant which has been known for various beneficial properties, such as the inhibition of bacterial growth and health enhancement properties. This study was carried out to evaluate the antibacterial effects, polyphenol contents and antioxidant activities of extracts from Andrographis paniculata plant materials, so as to partly explain its traditional application. Plant materials were extracted with 6 solvents: hot water, ethanol, methanol, ethyl acetate, acetone and hexane. We applied the agar-well-diffusion method to evaluate the inhibitory effects of extracts on several bacteria strains, including: Bacillus subtilis ATCC 6633; Geobacillus stearothermophilus ATCC 7953; Pseudomonas aeruginosa ATCC 9027; Staphylococcus aureus ATCC 25023; Staphylococcus aureus ATCC 25923; Escherichia coli ATCC 85922; Escherichia coli ATCC 35218; Escherichia coli ATCC 25922 and Salmonella ATCC 13311. Reagents, such as Folin Ciocalteu and 1,1-diphenyl-2-picrylhydrazyl (DPPH), were used to measure the polyphenol contents and antioxidant activities of the extracts, which were then respectedly converted to mg acid chlorogenic equivelent and mg VTME equivelent per 100 mg plant powder. The results showed that extracts from Andrographis paniculata showed antibacterial effects, in which the ethanol extract showed the highest antibacterial effects on bacteria, as it induced inhibitory zones on all testes bacteria, with the largest one was on Escherichia coli ATCC 25922 (18.81±2.22 mm). Extracts with high polyphenol contents and antioxidant activities were methanol, hot water and ethanol, in which the highest polyphenol content belonged to methanol extract (with the value reached to 0.270±0.019 mg acid chlorogenic equivalent per 100 mg plant powder), and the strongest antioxidant activity belong to hot water extract (with the value reached to 0.691±0.115 mg VTME equivalent per 100 mg plant powder).","PeriodicalId":166965,"journal":{"name":"JOURNAL OF CONTROL VACCINE AND BIOLOGICALS","volume":"290 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132636522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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