Journal of paramedical sciences最新文献_第2页

Molecular Identification of Members of Campylobacteriaceae Family in Gallstone 胆结石弯曲杆菌科成员的分子鉴定

Journal of paramedical sciences Pub Date : 2018-12-25 DOI: 10.22037/JPS.V9I4.18352

N. Zahed, M. Ghane

{"title":"Molecular Identification of Members of Campylobacteriaceae Family in Gallstone","authors":"N. Zahed, M. Ghane","doi":"10.22037/JPS.V9I4.18352","DOIUrl":"https://doi.org/10.22037/JPS.V9I4.18352","url":null,"abstract":"Introduction : The role of Bacterial infections as one major cause of occurrence of gallstones has been admitted. Campylobacteraceae family consists of Helicobacter , Campylobacter and Arcobacter genus have been identified as significant bacteria in the appearance of gastric disorders. This study aimed to investigate the frequency of Campylobacteriaceae family bacteria in the gallstones of patients hospitalized in the surgery ward of Shahid Rajaei hospital in Tonekabon. Materials and Methods : Sample of gallbladder stone was collected form 36 patients. After culture in the BHI medium for the primary enrichment, DNA extraction was carried out and then, the presence of the desired bacteria was examined by PCR technique. The obtained data was analyzed by SPSS software (21) and Chi-square (x 2 ) test. Results : Of total 36 samples of the studied gallstone, 3 samples (8.33%) were positive from viewpoint of presence of Helicobacter , 5 samples (13.88%) were positive in terms of presence of Campylobacter and only 1 sample (2.77%) was positive with respect to the presence of Arcobacter . No significant relationship was observed between type of stone and presence of these bacteria. Conclusion : The results achieved from this research show the presence of DNA belonging to the Campylobacteraceae family in the gallbladder stone, using PCR technique. These bacteria have an etiological significance in the formation of the gallstones. Therefore, more studies are required to determine the role of these bacteria in the formation of gallbladder stone.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"49 1","pages":"17-23"},"PeriodicalIF":0.0,"publicationDate":"2018-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72886306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Bcl2 gene expression in MCF-7 Human Breast Cancer Cells under treatment of Centaurea Behen extract and Cisplatin 半人马提取物和顺铂对MCF-7人乳腺癌细胞Bcl2基因表达的影响

Journal of paramedical sciences Pub Date : 2018-12-23 DOI: 10.22037/JPS.V9I4.22430

M. Entezari, S. Sheikhan, Z. Khazaie

{"title":"Evaluation of Bcl2 gene expression in MCF-7 Human Breast Cancer Cells under treatment of Centaurea Behen extract and Cisplatin","authors":"M. Entezari, S. Sheikhan, Z. Khazaie","doi":"10.22037/JPS.V9I4.22430","DOIUrl":"https://doi.org/10.22037/JPS.V9I4.22430","url":null,"abstract":"Introduction : Breast cancer is among the most common malignancies of human around the world. Millions of cases of cancer worldwide occur annually, which, if detected in a timely manner, are easier to treat and can be conveniently controlled. In 2008, about 1,384,155 breast cancer cases were detected worldwide, with about 459,000 of them deceased. Method :MTT assay was performed to evaluate cell proliferation.The BCL2 gene is an inhibitor of apoptosis that prevents the release of cytochrome C from mitochondria and leads to inhibition of various apoptosis stimuli. Due to the importance of this gene in the apoptotic process, the level of BCL2 gene expression under treatment of Cisplatin and Centaurea Behen agents for 24 hours and 48 hours was evaluated in this research,using the Real time-PCR method. It is noteworthy that Cisplatin as a DNA binding agent may be effective in treating breast cancer;moreover, some studies have shown that Centaurea Behenplant has an antioxidant effect that can be a preventive factor in cancer. Results : The results obtained related to Cisplatin showed that IC50 for cells treated with Cisplatin for 24 hours was about 2.91 mg/ml while IC50 for cells treated with Cisplatin for 48 hours was about 1.77 mg/ml. Similarly, results obtained related to Centaurea Behenherbal extract showed that IC50 for cells treated with Centaurea Behen for 24 hours was about 9.64 mg/ml while IC50 for cells treated with Centaurea Behen for 48 hours was about 7.85 mg/ml.Results showed that the expression level of gene under treatment of the Cisplatinand Centaurea Behenhas decreased compared to the non-treatment state, so this expression reduction showed a significant difference between samples group and control group . .","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"43 1","pages":"11-16"},"PeriodicalIF":0.0,"publicationDate":"2018-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73705624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The effect of crocin on total sleep-deprivation induced amnesia in male Wistar rats 藏红花素对全睡眠剥夺致雄性Wistar大鼠健忘症的影响

Journal of paramedical sciences Pub Date : 2018-12-23 DOI: 10.22037/JPS.V9I4.22553

Malihe Lootibashiyan, M. Nasehi, Solmaz Khalifeh, M. Zarrindast

{"title":"The effect of crocin on total sleep-deprivation induced amnesia in male Wistar rats","authors":"Malihe Lootibashiyan, M. Nasehi, Solmaz Khalifeh, M. Zarrindast","doi":"10.22037/JPS.V9I4.22553","DOIUrl":"https://doi.org/10.22037/JPS.V9I4.22553","url":null,"abstract":"Introduction: In recent years, Crocin has been used for its pharmacological functions, such as memory and learning enhancement. The aim of the present study was to assess the effect of Crocin on total sleep deprivation (TSD)-induced amnesia in male Wistar rats. Materials and Methods: The water box apparatus was used to induce sleep deprivation followed by Y- maze task as an index of learning and memory (the percentage of time in the novel arm during the retention phase was reported as an index of memory performances). The rats were divided into 12 groups, 8 rats in each group, including four control groups, four sham groups, and four TSD groups. Each group received saline and Crocin at doses of 1, 5 or 15 mg/kg twice a day. Results: The findings revealed that TSD for 24 h impaired memory function. In addition, the intra-peritoneal injection of Crocin at all doses (1, 5 and 15 mg/kg) did not change the percentage of time spent in the novel arm of Y-maze in sham of TSD, whereas it abolished the responses induced by the TSD groups. Conclusion: The findings showed a close interaction between the Crocin and SD. Based on the findings, Crocin seems to possess a modulatory effect on SD-induced amnesia.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"97 1","pages":"3-10"},"PeriodicalIF":0.0,"publicationDate":"2018-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76732584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A study on hospital blood information systems 医院血液信息系统的研究

Journal of paramedical sciences Pub Date : 2018-09-26 DOI: 10.22037/JPS.V9I3.22023

F. Asadi, Azamossadat Hosseini, Alireza Kazemi, F. Rahimi, Seylan Ghanyan

{"title":"A study on hospital blood information systems","authors":"F. Asadi, Azamossadat Hosseini, Alireza Kazemi, F. Rahimi, Seylan Ghanyan","doi":"10.22037/JPS.V9I3.22023","DOIUrl":"https://doi.org/10.22037/JPS.V9I3.22023","url":null,"abstract":"The blood bank unit is one of the most important and fundamental units in every hospital especially in emergency situations. Lack of access to medical records related to individual’s blood transfusion can postpone blood transfusion when it is essentially needed which can in turn increase patients' risks. Meanwhile, the role of a blood bank information system is crucial in accessing this information, thereby improving the quality of available care. This research was conducted to determine the status of the blood bank information system of the hospitals affiliated to Shahid Beheshti University of Medical Sciences.This descriptive- practical study investigated the blood bank information systems of 11 hospitals affiliated to Shahid Beheshti University of Medical Sciences in 2015. Data collection was performed through observation and interview, using a checklist and questionnaire. The validity of the instrument was determined through content validity and its reliability was confirmed through retesting. Data analysis was conducted using descriptive statistics.All blood bank information systems under investigation were semi-mechanized. It was impossible to send the full reports electronically and to present dynamic reports. The blood bank staff were not aware of the existence and significance of the relevant standards and no specific blood bank software were used.Mechanized blood bank information systems bring about effective communication in various health care organizations. They lead to the application of relevant novel technologies while increasing patients’ safety and satisfaction.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"220 6 1","pages":"56-62"},"PeriodicalIF":0.0,"publicationDate":"2018-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90768435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of increased manufacturing rate on risk of low back pain 增加制造率对腰痛风险的影响

Journal of paramedical sciences Pub Date : 2018-09-26 DOI: 10.22037/JPS.V9I3.18466

Saeid Ahmadi, S. Zare, Reza Ghanbari, S. Bahrami, V. Zaroushani

{"title":"The effect of increased manufacturing rate on risk of low back pain","authors":"Saeid Ahmadi, S. Zare, Reza Ghanbari, S. Bahrami, V. Zaroushani","doi":"10.22037/JPS.V9I3.18466","DOIUrl":"https://doi.org/10.22037/JPS.V9I3.18466","url":null,"abstract":"The blood bank unit is one of the most important and fundamental units in every hospital especially in emergency situations. Lack of access to medical records related to individual’s blood transfusion can postpone blood transfusion when it is essentially needed which can in turn increase patients' risks. Meanwhile, the role of a blood bank information system is crucial in accessing this information, thereby improving the quality of available care. This research was conducted to determine the status of the blood bank information system of the hospitals affiliated to Shahid Beheshti University of Medical Sciences.This descriptive- practical study investigated the blood bank information systems of 11 hospitals affiliated to Shahid Beheshti University of Medical Sciences in 2015. Data collection was performed through observation and interview, using a checklist and questionnaire. The validity of the instrument was determined through content validity and its reliability was confirmed through retesting. Data analysis was conducted using descriptive statistics.All blood bank information systems under investigation were semi-mechanized. It was impossible to send the full reports electronically and to present dynamic reports. The blood bank staff were not aware of the existence and significance of the relevant standards and no specific blood bank software were used.Mechanized blood bank information systems bring about effective communication in various health care organizations. They lead to the application of relevant novel technologies while increasing patients’ safety and satisfaction.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"11 1","pages":"49-55"},"PeriodicalIF":0.0,"publicationDate":"2018-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73064690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antimicrobial effects of Zingiber officinale extracts against multi-drug resistant Acinetobacter baumannii clinical isolates recovered from hospitalized patients in ICU 生姜提取物对ICU住院患者鲍曼不动杆菌临床分离株的抗菌作用

Journal of paramedical sciences Pub Date : 2018-07-18 DOI: 10.22037/JPS.V9I3.18624

Shirin HassasYeganeh, Seyed Reza Hosseini Doost, M. Goudarzi

{"title":"Antimicrobial effects of Zingiber officinale extracts against multi-drug resistant Acinetobacter baumannii clinical isolates recovered from hospitalized patients in ICU","authors":"Shirin HassasYeganeh, Seyed Reza Hosseini Doost, M. Goudarzi","doi":"10.22037/JPS.V9I3.18624","DOIUrl":"https://doi.org/10.22037/JPS.V9I3.18624","url":null,"abstract":"Zingiber officinale as an herbal medicinal plant is used for its potential antimicrobial activity against different microbial pathogens. Multi-drug resistant Acinetobacter baumannii as an important nosocomial pathogen especially in intensive care units is responsible for a wide range of serious infections in humans. The purpose of this study was to investigate the antimicrobial activity of Z. officinale extracts on growth of A. baumannii strains isolated from hospitalized patients in intensive care units in Tehran, Iran. During a 9 month study, 50 multi-drug resistant A. baumannii isolates were recovered from patients in ICU. The Kirby-Bauer disk diffusion method was used to determine resistance patterns of Multi-drug resistant A. baumannii isolates to antimicrobial agents. Micro-broth dilution method was used to determine the antimicrobial activity of methanol, acetone, and chloroform extracts of Z. officinale against multi-drug resistant A. baumannii isolates. The results of susceptibility testing showed that all the isolates were resistant to ceftriaxone, ciprofloxacin, ceftazidime, cefotaxime, cefepime and piperacillin. Resistance to colistin was found to be low (4%) and exhibited good antibacterial activity against tested isolates. This study’s findings revealed that methanol, acetone, and chloroform extracts of Z. officinale have anti-bacterial activity against tested bacterial isolates. Based on the results, the chloroform extracted fraction showed the highest level of activity at a minimal inhibitory concentration of 25 mg/ml on multi-drug resistant A. baumannii (64%). The minimal inhibitory concentration of ginger extract was as low as 3.2 mg/ml. The present study indicated that Z. officinale extracts, at various concentrations could be used as an antibacterial agent for treatment of patients in ICUs.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"50 1","pages":"42-48"},"PeriodicalIF":0.0,"publicationDate":"2018-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79412044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Credibility of health websites on infectious diseases: Are there any fully trustable website to read on Ebola? 关于传染病的健康网站的可信度:关于埃博拉的网站是否值得完全信任?

Journal of paramedical sciences Pub Date : 2018-07-15 DOI: 10.22037/JPS.V9I3.19330

Saeideh Valizadeh-Haghi, S. Rahmatizadeh, M. Ansari, Ronak Hamzehei

{"title":"Credibility of health websites on infectious diseases: Are there any fully trustable website to read on Ebola?","authors":"Saeideh Valizadeh-Haghi, S. Rahmatizadeh, M. Ansari, Ronak Hamzehei","doi":"10.22037/JPS.V9I3.19330","DOIUrl":"https://doi.org/10.22037/JPS.V9I3.19330","url":null,"abstract":"When a new disease develops, people are looking for information about that disease where the internet is considered as one of the sources of health information. Beside empowering individuals to identify and select valid information, reliable and up-to-date websites should also be introduced for them. Therefore, the present study aimed to evaluate and identify the high quality websites on Ebola virus and disease. To do this study, the term \"Ebola\" was searched for in Google, Yahoo, and Bing search engines using the Google Chrome browser. Three pages were obtained from the search in these three selected search engines and were examined, with the exclusion of duplicate websites, non-English websites, the results related to news, images, ads, and inaccessible websites; finally, 43 websites out of the 90 retrieved websites were selected for evaluation. The research tools included the HONcode toolbar and an eight-item checklist was developed by the researchers based on the HONcode of conducts. The result showed that out of 43 websites evaluated, only 6 websites (14%) were trustable and the 86% were not approved after evaluation based on the HONcode criteria. Only 37% of evaluated websites have been considered complementarity criterion. Justifiability was the most considered criterion and 95% of evaluated websites had paid to distribute information fairly enough. Online information about Ebola is available on many websites and this information affects people's health decisions and behaviors. Physicians and other health professionals can help patients and other community members to find their needed information on infectious diseases (e.g. on Ebola) from the most reputable and valid international websites.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"61 1","pages":"23-31"},"PeriodicalIF":0.0,"publicationDate":"2018-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81309008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dosimetric verification of the Elekta motorized wedge Elekta电动楔的剂量学验证

Journal of paramedical sciences Pub Date : 2018-07-15 DOI: 10.22037/JPS.V9I3.20231

Mahdie Behjati, Mostafa Sohrabpour, S. Shirmardi, F. Bouzarjomehri, M. A. M. Shirazi

{"title":"Dosimetric verification of the Elekta motorized wedge","authors":"Mahdie Behjati, Mostafa Sohrabpour, S. Shirmardi, F. Bouzarjomehri, M. A. M. Shirazi","doi":"10.22037/JPS.V9I3.20231","DOIUrl":"https://doi.org/10.22037/JPS.V9I3.20231","url":null,"abstract":"Elekta linear accelerator is equipped with a motorized wedge which produces the wedge angles of less than 60° continuously by the combination of a wedged field and an open field with appropriate proportions. The effective wedge angles for various field sizes and depths were calculated using an analytical formula and a 2-D array detector data. The validity of the effective wedge angles has been done by determining wedge angles in accordance with ICRU-24. The effect of the field size on the wedge angle and wedge factor was investigated for motorized wedge. The maximum difference between planned and measured angles was found to be about 10. The planned dose for different wedge angles and field sizes compared with measured doses and their differences were found to be less than 3%. The calculating wedge factor throughout linear interpolation method for all field sizes from a few selected measurements had been proved for physical wedge beforehand. This method was applied to obtain the wedge factors with field size and compared with measured data for motorized wedge. The errors were in agreement with ICRU proposed error and less than treatment planning system error. The linear relation between wedge angle and output factor and depth were investigated and the linear interpolation method was proved to calculate wedge angle for any output factor and depth.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"7 1","pages":"32-41"},"PeriodicalIF":0.0,"publicationDate":"2018-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84276076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

“Do the editors-in-chief of Iranian medical journals have a good Knowledge, attitude and practice of plagiarism?” “伊朗医学期刊的主编是否对抄袭有良好的认识、态度和做法?”

Journal of paramedical sciences Pub Date : 2018-07-03 DOI: 10.22037/JPS.V9I3.20140

Zohreh Abbasiyan, M. Shekofteh, Ebrahim Afshar, E. Ghasemi, F. Rahimi

{"title":"“Do the editors-in-chief of Iranian medical journals have a good Knowledge, attitude and practice of plagiarism?”","authors":"Zohreh Abbasiyan, M. Shekofteh, Ebrahim Afshar, E. Ghasemi, F. Rahimi","doi":"10.22037/JPS.V9I3.20140","DOIUrl":"https://doi.org/10.22037/JPS.V9I3.20140","url":null,"abstract":"Plagiarism is one of the misconducts which are specifically observed in scientific journals. It is important to prevent and reduce it in scientific communities. The chief editors of journals can play an effective role in this regard. Therefore, this study aims to determine the knowledge, attitude, and the practice (KAP) of the chief editors of scholarly journals in Universities of Iran about plagiarism. The data for this descriptive survey were collected through a researcher-made questionnaire which was developed and validated in three sections of knowledge, attitude, and practice. The statistical population of the study included all the chief editors of scholarly journals in Universities of Medical Sciences in Tehran. The scores of the editors were calculated separately in each section. The data collection procedure was followed either by email or in-person. The collected data were analyzed through SPSS. The chief editors’ score is %88.8 in the section of “knowledge of plagiarism” which represents a high score. The chief editors’ “attitude toward plagiarism” indicates a high level (%61.3), which represents a negative attitude of plagiarism, and their “practice” is also at a mid-level approaching towards high level. In general, the knowledge, attitude, and practice of chief editors about plagiarism are in a better status comparing to similar studies. However, it is expected that the editors in chief achieve a more favorable level in this regard.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"99 1","pages":"16-22"},"PeriodicalIF":0.0,"publicationDate":"2018-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85811513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Antibacterial and anticancer activity of a bioflavonoid fractionated from Allium Ascalonicum 薤白生物类黄酮的抑菌抗癌活性研究

Journal of paramedical sciences Pub Date : 2018-07-03 DOI: 10.22037/JPS.V9I3.19408

M. Amin, A. Mohammadi, Mohsen Heidary, S. Khoshnood

{"title":"Antibacterial and anticancer activity of a bioflavonoid fractionated from Allium Ascalonicum","authors":"M. Amin, A. Mohammadi, Mohsen Heidary, S. Khoshnood","doi":"10.22037/JPS.V9I3.19408","DOIUrl":"https://doi.org/10.22037/JPS.V9I3.19408","url":null,"abstract":"Allium ascalonicum is a part of the diet of many populations of the world due to their long-held beliefs. A. ascalonicum extracts have been reported have antibacterial properties and prevent cancer cell proliferation. This study was conducted for the purpse of evaluating the anticancer and antibacterial activity of a flavonoid fraction isolated from A. ascalonicum bulbs. The HeLa and HUVEC cells were used as target cell line and some gram negative and positive bacteria were also targeted for antimicrobial activity. The A. ascalonicum plant was collected from the Zagros Mountains in the north of Dezful city- Iran, in September 2016 and confirmed by School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. The water extract of bulbs of this plant was extracted and the flavonoid fraction was isolated from aquous extract by ethyl acetate. The antibacterial and anticancer effects of isolated falavonoid were determined using MIC and MTT respectively. The best antibacterial effect of falvonoid extracted from A.ascalonicum was found against C. diphtheria . Furthermore, gentamicin resistant P.aeruginosa was the most resistant pathogenic bacterium. The MTT method showed that this fraction had a concentration-dependent anti-proliferative activity on HeLa cell lines and there was no cytotoxic effect against HUVEC cells. The inhibitory concentration 50% (IC50) values of the A. ascalonicum extract for Hela cell was 3 mg/mL but the treatment of HUVECs with the A. ascalonicum showed no considerable effect. The flavonoid fraction of A.ascalonicum bulbs had remarkable antibacterial and anticancer properties. Therefore, it could be used as an antibacterial and anticancer agent for control of cancers and infectious diseases.","PeriodicalId":16663,"journal":{"name":"Journal of paramedical sciences","volume":"125 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2018-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76908238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3