Journal of Citrus Pathology最新文献

{"title":"Abstracts 2019 Joint IOCV XXI and IRCHLB VI","authors":"Organization Iocv","doi":"10.5070/c46255309","DOIUrl":"https://doi.org/10.5070/c46255309","url":null,"abstract":"","PeriodicalId":166019,"journal":{"name":"Journal of Citrus Pathology","volume":"392 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116329758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of RNAscope™ assays for the in-situ localization of viroid RNA in plant tissue.","authors":"Daniel Stanton, S. Harper, S. Cowell, R. Brlansky","doi":"10.5070/c48153251","DOIUrl":"https://doi.org/10.5070/c48153251","url":null,"abstract":"35 Due to their small size, locating pathogenic RNAs, such as viroids, in plant tissue and cell 36 organelles has been difficult. Viroids are small circular single-stranded RNA plant 37 pathogens that reduce plant growth, vigor, and yield in economically important crops 38 such as potato, tomato, hops and citrus. Viroid infections in plants are largely diagnosed 39 by dot blot hybridization, polyacrylamide gel electrophoresis (PAGE) or gels, or real-time 40 quantitative polymerase chain reaction (qPCR). Because traditional plant in situ 41 hybridization studies for viroids are often limited by the lack of signal amplification and 42 binding specificity due to the small target sequence, we examined the use of RNAscope™ 43 (Advanced Cell Diagnostics Inc., Newark, CA). This in situ hybridization method increases 44 the detection by amplifying the signal of a single target, to detect the cellular distribution 45 of citrus exocortis viroid (CEVd) with higher sensitivity and specificity. We found that 46 after optimization, CEVd was localized in nuclei of infected cells as clearly distinguishable 47 punctate red dots visible with light microscopy at low magnification, suggesting that the 48 RNAscope™ can be used to study viroids in situ . 49 50","PeriodicalId":166019,"journal":{"name":"Journal of Citrus Pathology","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125162825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Program of the 3rd International Research Conference on Huanglongbing (IRCHLB-III), Feb. 4-8, 2013, Orlando, Florida, U.S.A.","authors":"Committee Organizing","doi":"10.5070/c41155310","DOIUrl":"https://doi.org/10.5070/c41155310","url":null,"abstract":"","PeriodicalId":166019,"journal":{"name":"Journal of Citrus Pathology","volume":"144 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114524327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of citrus virus A in citrus in South Africa","authors":"R. Bester, M. Karaan, G. Cook, H. J. Maree","doi":"10.5070/C481049000","DOIUrl":"https://doi.org/10.5070/C481049000","url":null,"abstract":"Author(s): Bester, Rachelle; Karaan, Maryam; Cook, Glynnis; Maree, Hans J | Abstract: High-throughput sequencing (HTS) of citrus indicator hosts, inoculated from psorosis-like diseased samples, revealed the presence of the first South African variant of citrus virus A (CiVA).n This virus was first identified in citrus in Italy and is the second member of the Coguvirus genus within the order Bunyavirales. The South African sequence variants of CiVA shares 95.55% (RNA1) and 94.82% (RNA2) nucleotide sequence identity with the Italian CiVA sequence. The discovery of CiVA in South African citrus orchards indicates the global distribution of CiVA and further investigations are required to determine the association of CiVA with psorosis-like disease symptoms.","PeriodicalId":166019,"journal":{"name":"Journal of Citrus Pathology","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128202564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Citrus dwarfing viroid reduces canopy volume by affecting shoot apical growth of navel orange trees grown on trifoliate orange rootstock","authors":"I. Lavagi-Craddock, R. Campos, D. Pagliaccia, T. Kapaun, C. Lovatt, G. Vidalakis","doi":"10.5070/C471045369","DOIUrl":"https://doi.org/10.5070/C471045369","url":null,"abstract":"Author(s): Lavagi-Craddock, I; Campos, R; Pagliaccia, D; Kapaun, T; Lovatt, C; Vidalakis, G | Abstract: Citrus dwarfing viroid (CDVd) infection of navel orange trees (Citrus sinensis (L.) Osb.) on ‘Rich 16-6’ trifoliate orange (Poncirus trifoliata (L.) Raf.) rootstock has been previously reported to reduce canopy volume by approximately 50%. We demonstrated that the reduction in tree size of CDVd-infected citrus resulted from a g20% reduction in the apical growth of individual shoots within the tree canopy. We also demonstrated that the reduced canopy volume of the CDVd-infected trees is a long lasting phenotype comparable to that of ‘Flying Dragon’ rootstock, which is known to reduce citrus tree size.","PeriodicalId":166019,"journal":{"name":"Journal of Citrus Pathology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122177982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generating Asian citrus Psyllid Diaphorina citri Kuwayama (Homoptera: Psyllidae) with twisting wings to prevent the spread of citrus greening disease","authors":"Ibrahim A. El-Shesheny, S. Harjeri, S. Gowda, N. Killiny","doi":"10.5070/C411025089","DOIUrl":"https://doi.org/10.5070/C411025089","url":null,"abstract":"Huanglongbing (HLB) is seriously threatening and causing considerable economic losses to the citrus groves. Its Management depends critically on the control of the Asian citrus Psyllid (ACP), the vector of the cause of HLB, Candidatus Liberibacter asiaticus bacteria (CLas). Silencing genes by RNA interference (RNAi) is a promising technique to control pests. In this study, the abnormal disk wing (awd) has been selected from the available psyllid annotated genome. It has been known that awd gene encodes a nucleoside diphosphate kinase and is associated with wing development. This research focused on the effect of RNAi of awd gene on ACP nymph instars that acquired dsRNA. The Results provide evidence that using the dsRNA of awd gene has diminished the development and survival of ACP nymphs. Moreover, knockdown of awd gene expression was observed through malformation of adult wings. Also, the expression of awd was messured by quantitative PCR (qPCR). Furthermore, we are conducting experiments to investigate awd's possible contribution in temperature tolerance. We attempt to establish effective practical application to prevent the spread of HLB in friendly environmentally strategy.","PeriodicalId":166019,"journal":{"name":"Journal of Citrus Pathology","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131709738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First comprehensive sanitary report of citrus-infecting viruses and viroids in Uruguay","authors":"M. J. Benítez-Galeano, L. Hernández-Rodríguez, F. Dalmao, E. Bertoni, Ana Bertalmío, L. Rubio, F. Rivas, D. Maeso, R. Colina","doi":"10.5070/C481049181","DOIUrl":"https://doi.org/10.5070/C481049181","url":null,"abstract":"Author(s): Benitez-Galeano, MJ; Hernandez-Rodriguez, L; Dalmao, F; Bertoni, E; Bertalmio, A; Rubio, L; Rivas, F; Maeso, D; Colina, R | Abstract: Citrusnis the highest-value fruit crop in terms of international trade.nHowever, citrus species are susceptible to several diseases caused by different types of micro-organisms which directly cause a decrease in production leading to economic losses. Since the last half-century, citrus industry in Uruguay has a strong socio-economic impact and is constantly evolving to stay competitive, including strategies as the incorporation of new varieties and high yielding orchards. Nevertheless, despite the existence since 2010 of the National Citrus Sanitary and Certification Program, none or very little information about the sanitary status of these citrus plantings regarding viruses and viroids was available. The incidence ofncitrus tristeza virus (CTV),ncitrus psorosis virus (CPsV), satsuma dwarf virus (SDV),ncitrus exocortis viroid (CEVd),nhop stunt viroid (HSVd),ncitrus dwarfing viroid (CDVd),ncitrus bark cracking viroid (CBCVd)nandncitrus bent leaf viroid (CBLVd) was established, as well as CTV genotypes circulating in the country. Using molecular biology methods, 1175 samples from Valencia and Navel sweet oranges, Afourer and Clementine mandarins, and lemons, randomly collected from seven citrus-producing departments were analysed. Only 6% of the sampled citrus plants were pathogen-free, while 93% of them were CTV positive. SDV, CBLVd and CBCVd were not detected. Co-infections were very frequent finding plants with as many as four pathogens at the same time, being CTV present in all co-infected plants. This is the first comprehensive sanitary report of several citrus-infecting viruses and viroids in Uruguayan citrus groves, as well as the main CTV circulating genotypes in the country.","PeriodicalId":166019,"journal":{"name":"Journal of Citrus Pathology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134252181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why we should care about culturing the Huanglongbing associated bacterium ‘Candidatus Liberibacter asiaticus’: the importance of terms and interpretations","authors":"M. Merfa, L. Fuente","doi":"10.5070/C471050303","DOIUrl":"https://doi.org/10.5070/C471050303","url":null,"abstract":"Author(s): Merfa, Marcus Vinicius; De La Fuente, Leonardo | Abstract: Having bacteria grown in pure culture has been the foundation of bacteriology, by allowing a wide range of microbiological studies towards understanding the functionality of a specific bacterium. However, most bacteria have not been axenically cultured to date, thus hindering the understanding of their role in the context of their host or environment. One of these unculturable bacteria are the recently emergent plant pathogens ‘Candidatus Liberibacter spp.’. This group is comprised by dynamic psyllid-vectored, phloem-limited plant pathogens and endophytes that harm a wide range of economically important crops worldwide. ‘Candidatus Liberibacter asiaticus’ (CLas) is associated with Huanglongbing (HLB) in most of the main citrus-producing areas globally, a disease causing severe economic damages. Although the establishment of axenic cultures of CLas remains a major scientific challenge, many research groups have devoted efforts to culture this bacterium to aid in elucidating its virulence mechanism and contribute towards effective HLB management. This led to the development of innovative systems to culture and grow CLas, however different authors have approached the concepts of bacterial culture and axenic culture in different manners, leading to confusion in the terminology used. In this review, we provide the scientific definitions of important terms in bacteriology, while critically reviewing the contribution of each of these important CLas culturing studies.","PeriodicalId":166019,"journal":{"name":"Journal of Citrus Pathology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123410454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Results on attempts in management of HLB under small scale in Vietnam and initiation in screening for HLB tolerant from varieties/clones belonging to Rutaceae","authors":"V. H. Nguyen, T. Tran, T. Nguyen, T. Bui, M. Nguyen","doi":"10.5070/C411025272","DOIUrl":"https://doi.org/10.5070/C411025272","url":null,"abstract":"In the Mekong Delta, Vietnam, Citrus Huanglongbing (HLB) was officially announced in 1994 and its causal organism was described by Bove and Garnier in 1995 to be Candidatus Liberibacter asiaticus. Throughout the years, intensive works have been carried out for HLB control under small scale orchards and the achievements are discussed, the model for effective control of HLB under small scale which could elongate the life cycle of citrus tree for better and longer harvesting. In addition, there were 130 rutaceae related accessions had been collected and screened for HLB tolerance; the results revealed that the serverity of HLB infection was less on Hanh/Tac/Quat (Citrus microcarpa) and Long Co co pummelo (Citrus maxima) than that on orange and mandarin. The wild Rutaceae species/clones such as Quyt Dang, Quyt rung, Cam rung, Buoi Rung, Buoi Dang, Buoi Bung, Mac Run, Mac Mat, Can Thang, Quach, Nguyet Que, Kim quit, Truc, Com Ruou, Dau dau ba la, Ca ri, Da tu bien and Gioi Lom were symptomless under transmission conditions and had a negative reaction by PCR tests. In a molecular study, 38 primers have been designed and used for screening of HLB tolerance capacity of 49 varieties/clones belonging to Rutaceae. The preliminary results shown that the tolerant varieties was grouped into Group D, which somehow matched with the tolerant group screened under screenhouse conditions.","PeriodicalId":166019,"journal":{"name":"Journal of Citrus Pathology","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121962146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A historical note on two unreported obstacles for cross-protecting mature citrus trees against severe Citrus tristeza virus isolates.","authors":"M. Bar-joseph","doi":"10.5070/C421028534","DOIUrl":"https://doi.org/10.5070/C421028534","url":null,"abstract":"Letter to the Editor A historical note on two unreported obstacles for cross-protecting mature citrus trees against severe Citrus tristeza virus isolates. M Bar-Joseph 1* The S Tolkowsky Laboratory, ARO, The Volcani Center, Bet Dagan 50250, Israel. *Correspondence to: mbjoseph@gmail.com Citation: Bar-Joseph M. 2015. A historical note on two unreported obstacles for cross-protecting mature citrus trees against severe Citrus tristeza virus isolates. J Cit Pathol. iocv_journalcitruspathology_28534. During the years 1970 to 1986 the Israeli citrus industry had undertaken a costly and ambitious program for suppressing the outbreak of a Citrus tristeza virus (CTV) epidemic. For comprehensive reviews of CTV and the tristeza disease see Dawson et al. (2013), Moreno et al. (2008), and Bar-Joseph et al. (1989). The program, which involved millions of ELISA tests, was a coordinated effort of virus research, extension, and regulatory agencies funded by the local citrus marketing board and the Ministry of Agriculture. These major commitments of CTV control by a “search and destroy” strategy were based on statistical analyses indicating that CTV infection rates throughout citrus areas were low (Bar-Joseph et al. 1989). Because of the absence of reliable diagnostic methods to differentiate between mild and severe CTV isolates at early stages of infection, the program policy was to eliminate every tree that showed a positive ELISA reaction. An increase in the incidence of CTV detection during 1983 to 1985 and grower reluctance to cooperate with timely removal of symptomless infected trees, pointed to the need for reevaluating CTV infection rates. Results in 1986 suggested that despite the suppression efforts, which for economic reasons had been already reduced a few years earlier, there were about 50,000 undetected infected trees, spread over 5000 hectares (Bar-Joseph et al. 1989). It was also apparent from the high ratio of symptomless to declining trees that the majority of these sweet orange on sour orange rootstock trees were carriers of “mild” CTV isolates. Trees infected with such isolates remained symptomless for 5 to 10 years, even when the isolates were infecting a decline sensitive combination of sweet orange scions on sour orange rootstocks. Cost-benefit analyses indicated that locating these symptomless trees among the millions of citrus trees cultivated at that time in Israel would have involved testing and compensation costs far beyond the industry’s funding resources. Once the CTV suppression program came to a halt, testing the mild isolates from symptomless trees for their ability to protect trees in plots with severe decline causing isolates became an option. In 1988 a cross protection iocv_journalcitruspathology_28534 experiment to prevent decline was established in a mature (around 20 years old) Valencia orange grove grafted on sour orange. The experimental plot was part of the Yachin Company Morasha plantation located east of Tel Aviv. In thi","PeriodicalId":166019,"journal":{"name":"Journal of Citrus Pathology","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116987428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}