Revista de Investigaciones Veterinarias del Perú最新文献_第2页

Efecto del suplemento Insulina-Transferrina-Selenio (ITS) sobre la tasa de desarrollo y calidad de embriones bovinos in vitro 补充胰岛素-转铁蛋白-硒（ITS）对牛体外胚胎发育速度和质量的影响

Revista de Investigaciones Veterinarias del Perú Pub Date : 2024-02-29 DOI: 10.15381/rivep.v35i1.25348

Mariano Eliécer Acosta Lobo, Pablo Andrés Robledo Salgado, Luis Fernando Londoño Franco, Viviana Andrea Torres Osorio, Neil A. Vásquez Araque

{"title":"Efecto del suplemento Insulina-Transferrina-Selenio (ITS) sobre la tasa de desarrollo y calidad de embriones bovinos in vitro","authors":"Mariano Eliécer Acosta Lobo, Pablo Andrés Robledo Salgado, Luis Fernando Londoño Franco, Viviana Andrea Torres Osorio, Neil A. Vásquez Araque","doi":"10.15381/rivep.v35i1.25348","DOIUrl":"https://doi.org/10.15381/rivep.v35i1.25348","url":null,"abstract":"The aim of this study was to evaluate the effect of Insulin-Transferrin-Selenium (ITS) supplementation in embryo culture on the blastocyst rate and embryo quality. The bovine zygotes obtained by in vitro maturation (MIV) and fertilization (IVF) were transferred to Evolve medium in 4 groups (T1: Without ITS supplement; T2: ITS the first 48 h of culture (CIV1): T3: ITS after day 2 of culture (CIV2), T4: ITS during all culture time (CIV1 + CIV2). All procedures were performed at 38.5 °C, 5% CO2 and 90% relative humidity. The cleavage and blastocyst rates were determined at 48 h and day 8 of culture. The total cell number and the production of reactive oxygen species (ROS) in the expanded blastocysts were determined by staining the nucleus with Hoechst and the oxidation of the fluorescent probe dichlorodihydrofluorescein diacetate (H2DCFDA). The T4 cultured embryos presented the highest rate of blastocysts (32.6%) and total cell number (145.6) compared to the control group without STI (26.2% and 98.6, respectively; p<0.05). Likewise, the production of ROS in T4 blastocysts was lower (p<0.05) than that obtained in the other study groups. The results show that the addition of the ITS supplement throughout the embryo culture time has beneficial effects on the percentage of blastocysts and embryo quality, assessed by the number of total cells and the production of ROS.","PeriodicalId":163899,"journal":{"name":"Revista de Investigaciones Veterinarias del Perú","volume":"23 50","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140408874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the symmetry of bovine thyroid gland using geometric morphologic techniques 利用几何形态学技术评估牛甲状腺的对称性

Revista de Investigaciones Veterinarias del Perú Pub Date : 2024-02-29 DOI: 10.15381/rivep.v35i1.25485

P. M. Parés-Casanova

引用次数: 0

Effect of delayed addition of fetal calf serum to the culture medium on in vitro embryo production from creole heifers raised in a high altitude in Ecuador 在培养基中延迟添加胎牛血清对厄瓜多尔高海拔地区克里奥尔母牛体外胚胎生产的影响

Revista de Investigaciones Veterinarias del Perú Pub Date : 2024-02-29 DOI: 10.15381/rivep.v35i1.25307

Maria S. Méndez, D. Argudo, Manuel E. Soria, Luis Galarza, Diego A. Galarza, Ricardo H. Alberio, F. Perea

{"title":"Effect of delayed addition of fetal calf serum to the culture medium on in vitro embryo production from creole heifers raised in a high altitude in Ecuador","authors":"Maria S. Méndez, D. Argudo, Manuel E. Soria, Luis Galarza, Diego A. Galarza, Ricardo H. Alberio, F. Perea","doi":"10.15381/rivep.v35i1.25307","DOIUrl":"https://doi.org/10.15381/rivep.v35i1.25307","url":null,"abstract":"El estudio tuvo como objetivo evaluar el efecto del suero fetal bovino (FCS) agregado al medio de cultivo los días 5 a 7 después de la fertilización in vitro sobre la producción y posterior criotolerancia de blastocistos de vaquillonas criollas ecuatorianas. Se recolectaron complejos cúmulo-ovocitos (COCs) inmaduros mediante aspiración intravaginal guiada por ultrasonido (OPU) de 10 vaquillonas criollas y de ovarios de matadero en ocho sesiones. Los COCs se sometieron a maduración in vitro (MIV), fecundación (FIV) y cultivo (IVC). El día 1 después de la FIV, los presuntos embriones de cada fuente de ovocitos (OPU [O] y Matadero [A]) se asignaron aleatoriamente en dos grupos según si se añadió FCS al 2.5% (v/v) al medio de cultivo el día 5 después de la FIV: 1) O-FCS+, 2) O-FCS-, 3) A-FCS+ y 4) A-FCS-. El día 7 después de la FIV se vitrificaron embriones de alta calidad. La criotolerancia de los embriones vitrificados-calentados se evaluó según la reexpansión del blastocele a las 2 h y la posterior reexpansión y eclosión a las 24 y 48 h de incubación. La tasa de blastocistos el día 7 no difirió entre la fuente de ovocitos y el grupo FCS. Después del proceso de vitrificación/calentamiento, la adición de FCS solo afectó la tasa de reexpansión a las 2 h, independientemente de la fuente de ovocitos (p<0.05). Asimismo, la tasa de eclosión de los blastocistos a las 48 h de incubación se vio drásticamente afectada únicamente en los ovocitos derivados de OPU (p<0.01). En conclusión, la suplementación de FCS el día 5 después de la FIV no mejoró la producción de blastocistos y afectó negativamente la criotolerancia de embriones bovinos in vitro derivados de vaquillonas criollas criadas en los Andes ecuatorianos.","PeriodicalId":163899,"journal":{"name":"Revista de Investigaciones Veterinarias del Perú","volume":"24 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140414095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efecto de la manteca de cerdo criollo cajamarquino como reemplazo dietario de los aceites de soya y palma sobre la respuesta productiva y metabolitos plasmáticos del pavo 用 Cajamarca Criollo 猪油替代大豆油和棕榈油对火鸡生产反应和血浆代谢物的影响。

Revista de Investigaciones Veterinarias del Perú Pub Date : 2024-02-29 DOI: 10.15381/rivep.v35i1.27378

Manuel Paredes, Marx Quintana, J. Mantilla

{"title":"Efecto de la manteca de cerdo criollo cajamarquino como reemplazo dietario de los aceites de soya y palma sobre la respuesta productiva y metabolitos plasmáticos del pavo","authors":"Manuel Paredes, Marx Quintana, J. Mantilla","doi":"10.15381/rivep.v35i1.27378","DOIUrl":"https://doi.org/10.15381/rivep.v35i1.27378","url":null,"abstract":"The effect of three sources of lipids in the diet of fattening turkeys from the Hybrid Converter line on growth, carcass weight and blood metabolites were evaluated. A total of 90 43-day-old male turkeys were randomly distributed into 3 treatments, with 5 repetitions and 6 birds per group. The turkeys received isonutritional diets supplemented with Cajamarca Creole lard (MCCC), soybean oil (AS) or palm oil (AP). Turkeys aged 43 to 70 days with MCCC and AP had better body weight gain, lower feed consumption and better feed conversion (p<0.05) than those with the AS treatment; However, there were no differences between treatments during the finishing phase (71-98 days) nor in the total fattening period (43-98 days). The carcass weight in MCCC and AS was greater than in AP, in addition, they reduced (p<0.05) the relative weight of the liver and the percentage of abdominal fat compared to AP. Triglycerides and total cholesterol in blood were lower (p<0.05) in AS and MCCC than in AP, likewise, MCCC decreased (p<0.05) the malondialdehyde content in blood. It is concluded that MCCC in fattening turkeys from 43 to 98 days of age can replace AS and AP in the diet, since it does not generate negative effects or a decrease in the evaluated indicators.","PeriodicalId":163899,"journal":{"name":"Revista de Investigaciones Veterinarias del Perú","volume":"2019 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140415991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lateral ovariohysterectomy in female cats: an alternative for birth control programmes 雌猫卵巢外侧切除术：节育计划的替代方案

Revista de Investigaciones Veterinarias del Perú Pub Date : 2024-02-29 DOI: 10.15381/rivep.v35i1.25395

Jierson E. Mendoza-Estela

{"title":"Lateral ovariohysterectomy in female cats: an alternative for birth control programmes","authors":"Jierson E. Mendoza-Estela","doi":"10.15381/rivep.v35i1.25395","DOIUrl":"https://doi.org/10.15381/rivep.v35i1.25395","url":null,"abstract":"Ovariohysterectomy through the linea alba is one of the most applied surgical techniques in companion animals; however, this technique presents frequent postoperative complications. The present study aims to demonstrate the feasibility and advantages of ovariohysterectomy in cats through the lateral approach. In total, 30 clinically healthy cats weighing 2.9 ± 0.9 kg body weight, 1.5- 4 years old and of various breeds, were operated on. In the preanesthetic medication, xylazine hydrochloride (0.1 mg/kg) and ketamine hydrochloride (0.5 mg/kg) were applied IM, anesthesia was done with diazepam (0.5 mg/kg) and ketamine hydrochloride (10 mg/kg). IV route, and maintenance with boluses of ketamine hydrochloride (0.5 mg/kg) based on dose-response. A single dose of amoxicillin (10 mg/kg) was applied IM at the end of surgery. The time of the surgical intervention was 19.8 ± 2.5 minutes and for wound healing it was 6.9 ± 1.2 days. Two patients 6.7% (2/30) presented inflammation and local infection. It is concluded that ovariohysterectomy through a lateral approach in cats is a viable alternative due to its ease of execution, reduced healing time and minimal presentation of postoperative complications.","PeriodicalId":163899,"journal":{"name":"Revista de Investigaciones Veterinarias del Perú","volume":"2011 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140416648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cow behaviour and milk yield during different categories temperature-humidity indices 不同温湿度指数下的奶牛行为和产奶量

Revista de Investigaciones Veterinarias del Perú Pub Date : 2024-02-29 DOI: 10.15381/rivep.v35i1.25305

O. Borshch, S. Ruban, Oleksandr V. Borshch, Mykhailo Matvieiev, Vasyl Prudnikov, Oleksandr Sobolev, V. Bilkevych, Maksym Fedorchenko

引用次数: 0

Textile characteristics and fibre length at dehairing on vicuña fleece 驼绒的纺织特性和脱毛时的纤维长度

Revista de Investigaciones Veterinarias del Perú Pub Date : 2024-02-29 DOI: 10.15381/rivep.v35i1.27377

Rubén Pinares, Franklin Lozano, Virgilio Machaca

{"title":"Textile characteristics and fibre length at dehairing on vicuña fleece","authors":"Rubén Pinares, Franklin Lozano, Virgilio Machaca","doi":"10.15381/rivep.v35i1.27377","DOIUrl":"https://doi.org/10.15381/rivep.v35i1.27377","url":null,"abstract":"El objetivo de la investigación fue determinar la variación de las características textiles y longitud de fibra al descerdado manual del vellón de vicuña, así como evaluar la influencia del sexo, edad, sitio de muestreo del vellón, y lugar de procedencia de la vicuña sobre las características de calidad de la fibra. El descerdado manual se realizó en 77 muestras de vicuñas provenientes de tres provincias del departamento de Apurímac, Perú. Las características textiles de la fibra antes y después del descerdado fueron evaluadas usando el equipo OFDA 2000 y mediante el análisis de varianza, prueba de t y Tukey (α=0.05). La calidad de fibras descerdada es superior a la fibra no descerdada, debido a la reducción del diámetro medio de fibra (DMF) (-1.68 μm), desviación estándar de DMF (DE) (-0.73) y finura al hilado (FH) (-1.27 μm) y al incremento del porcentaje de fibras <30 µm (FC) (1.12%) y de la curvatura media (CU) (6.75°/mm). En fibra descerdada los juveniles tienen un DMF más fino (12.24 μm) que las adultas (13.01 μm), el DMF del costillar medio es más fino (12.39 μm) respecto a paleta (12.81 μm) y grupa (12.77 μm). Asimismo, se encontró variación de las características textiles (DE, CV, FC, y CU) y longitud de fibra entre los vellones de las tres provincias de Apurímac. En conclusión, el descerdado manual reduce el diámetro medio de fibra y su desviación estándar, y aumenta la curvatura media y el confort de la fibra, mejorando las propiedades de calidad y uniformidad del hilo.","PeriodicalId":163899,"journal":{"name":"Revista de Investigaciones Veterinarias del Perú","volume":"45 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140411539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluación biológica, fisicoquímica y de potencial tecnológico del tiburón azul (Prionace glauca) procedente del pacifico sur peruano 秘鲁南太平洋大青鲨（Prionace glauca）的生物、理化和技术潜力评估。

Revista de Investigaciones Veterinarias del Perú Pub Date : 2024-02-29 DOI: 10.15381/rivep.v35i1.27376

D. Pariona-Velarde, Andrés Reátegui-Quispe

{"title":"Evaluación biológica, fisicoquímica y de potencial tecnológico del tiburón azul (Prionace glauca) procedente del pacifico sur peruano","authors":"D. Pariona-Velarde, Andrés Reátegui-Quispe","doi":"10.15381/rivep.v35i1.27376","DOIUrl":"https://doi.org/10.15381/rivep.v35i1.27376","url":null,"abstract":"El objetivo del estudio fue evaluar las características biológicas, fisicoquímicas y tecnológicas relacionadas del tiburón azul Prionace glauca. Se trabajó con 128 especímenes para determinar sus propiedades biológicas, tecnológicas y 27 ejemplares para sus características fisicoquímicas. Se registraron los datos biométricos de longitud y peso (142 a 281 cm y 10.5 a 93.0 kg para machos; 153.5 a 255.0 cm y 14.5 a 80.0 kg para hembras), índice de alometría (machos 3.27 y hembras 2.87), potencial tecnológico mediante diferentes tipos de rendimientos (RED: 83.9±0.03%, RESV: 67.2±0.04%, RSCV: 48.9±0.07% y RPC: 33.5±0.07%) y capacidad de retención de agua en el músculo (16.1±0.03%). Dentro de las características fisicoquímicas se analizó la composición química proximal (proteína 21.8±0.23; grasa 0.6±0.03; humedad 78.0±0.31; ceniza 1.3±0.01) y los minerales (Cu: 2.0±0.11 mg/kg, Fe: 17.8±1.95 mg/kg, Zn: 25.8±1.76 mg/kg; Ca: 4.3±0.27 mg/100 g, Mg: 95.4±7.87 mg/100 g, Na: 716.3±24.03 mg/100 g, K: 1526.1±54.4 mg/100 g, P: 439.5±15.76 mg/100 g). No hubo evidencia de diferencias significativas en función al sexo. Con base a las comparaciones realizadas con bases de datos de recursos pesqueros, el músculo de tiburón azul no presenta ventajas comparativas relativos al rendimiento, perfil mineral o composición química proximal de otras especies.","PeriodicalId":163899,"journal":{"name":"Revista de Investigaciones Veterinarias del Perú","volume":"5 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140412914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of antimicrobial peptides from Enterococcus faecium IP5-2a isolated from tilapia gut using mass spectrometry 利用质谱法鉴定从罗非鱼肠道中分离出的粪肠球菌 IP5-2a 的抗菌肽

Revista de Investigaciones Veterinarias del Perú Pub Date : 2024-02-29 DOI: 10.15381/rivep.v35i1.25580

Arnaldo E. Castañeda, Manuel Feria Zevallos, Adrian E. Zatan, Odalis E. Toledo, Jorge L. Aguilar, P. Masías, Virna Cedeño, Benoit Diringer

引用次数: 0

Comparación de modelos matemáticos para describir la curva de lactancia en yeguas 描述母马泌乳曲线的数学模型比较

Revista de Investigaciones Veterinarias del Perú Pub Date : 2024-02-29 DOI: 10.15381/rivep.v35i1.25463

R. Rosero-Noguera, S. L. Posada-Ochoa, J. R. Martínez-Aranzales

引用次数: 0