Journal of Medical Entomology最新文献_第4页

Optimization of artificial membrane feeding system for lone star ticks, Amblyomma americanum (Acari: Ixodidae), and experimental infection with Rickettsia amblyommatis (Rickettsiales: Rickettsiaceae) 孤星蜱（Amblyomma americanum，Acari: Ixodidae）人工膜饲喂系统的优化及立克次体（Rickettsiales: Rickettsiaceae）的实验感染

IF 2.1 3区农林科学

Journal of Medical Entomology Pub Date : 2023-12-17 DOI: 10.1093/jme/tjad158

Ilia Rochlin, Dennis Chu, Matthew Gmelin, Justin Le, Martha B Furie, David G Thanassi, Hwan Keun Kim

{"title":"Optimization of artificial membrane feeding system for lone star ticks, Amblyomma americanum (Acari: Ixodidae), and experimental infection with Rickettsia amblyommatis (Rickettsiales: Rickettsiaceae)","authors":"Ilia Rochlin, Dennis Chu, Matthew Gmelin, Justin Le, Martha B Furie, David G Thanassi, Hwan Keun Kim","doi":"10.1093/jme/tjad158","DOIUrl":"https://doi.org/10.1093/jme/tjad158","url":null,"abstract":"With the introduction of siliconized artificial membranes, various artificial feeding systems (AFS) for hard ticks (Ixodidae) have been developed over the last decades. Most AFS utilize similar core components but employ diverse approaches, materials, and experimental conditions. Published work describes different combinations of the core components without experimental optimizations for the artificial feeding of different tick species. Amblyomma americanum L., (Acari: Ixodidae) (lone star tick) is a known vector and reservoir for diverse tick-borne pathogens, such as Rickettsia amblyommatis and Ehrlichia chaffeensis. Ongoing environmental changes have supported the expansion of A. americanum into new habitats, contributing to increased tick-borne diseases in endemic areas. However, a significant knowledge gap exists in understanding the underlying mechanisms involved in A. americanum interactions with tick-borne pathogens. Here, we performed a systematic analysis and developed an optimized AFS for nymphal lone star ticks. Our results demonstrate that Goldbeater’s membranes, rabbit hair, hair extract, and adult lone star ticks significantly improved the attachment rate of nymphal ticks, whereas tick frass and frass extract did not. With the optimized conditions, we achieved an attachment rate of 46 ± 3% and a success rate of 100% (i.e., one or more attached ticks) in each feeding experiment for nymphal lone star ticks. When fed on sheep blood spiked with R. amblyommatis, both nymphal and adult lone star ticks acquired and maintained R. amblyommatis, demonstrating the feasibility of studying A. americanum–pathogen interactions using AFS. Our study can serve as a roadmap to optimize and improve AFS for other medically relevant tick species.","PeriodicalId":16325,"journal":{"name":"Journal of Medical Entomology","volume":"16 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138743028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A preliminary investigation of rabbit carcass decomposition and attracted ants (Hymenoptera: Formicidae) on the seaward coastal beach of Al-Jubail City, Saudi Arabia 沙特阿拉伯 Al-Jubail 市沿海沙滩上兔子尸体腐烂和吸引蚂蚁（膜翅目：蚁科）的初步调查

IF 2.1 3区农林科学

Journal of Medical Entomology Pub Date : 2023-12-17 DOI: 10.1093/jme/tjad162

Afnan S Al-Qurashi, Ashraf M Mashaly, Reem Alagmi, Mohamed S Al-Khalifa, Lamjed Mansour, Suliman Y Al-Omar, Mostafa R Sharaf, Abdulrahman S Aldawood, Hathal M Al-Dhafer, Tony Hunter, Masha’el Y Almutawa, Ashraf M Ahmed

{"title":"A preliminary investigation of rabbit carcass decomposition and attracted ants (Hymenoptera: Formicidae) on the seaward coastal beach of Al-Jubail City, Saudi Arabia","authors":"Afnan S Al-Qurashi, Ashraf M Mashaly, Reem Alagmi, Mohamed S Al-Khalifa, Lamjed Mansour, Suliman Y Al-Omar, Mostafa R Sharaf, Abdulrahman S Aldawood, Hathal M Al-Dhafer, Tony Hunter, Masha’el Y Almutawa, Ashraf M Ahmed","doi":"10.1093/jme/tjad162","DOIUrl":"https://doi.org/10.1093/jme/tjad162","url":null,"abstract":"The current study was carried out in the seaward coastal beach environment of Al-Jubail City, Saudi Arabia, to analyze the rabbit carcass decomposition process, the succession pattern of associated ants, and their potential utility in forensic investigation. Experiments were conducted over a 4-season course (from autumn 2018 to summer 2019). A total of 9 species belonging to the 2 subfamilies, Myrmicinae and Formicinae, were recorded. The myrmicine species were Crematogaster aegyptiaca Mayr, 1862; Messor ebeninus Santschi, 1927; Messor foreli Santschi, 1923; and Monomorium abeillei Andre, 1881. The formicine species were Camponotus xerxes Forel, 1904; Cataglyphis albicans (Roger, 1859); Cataglyphis hologerseni Collingwood & Agosti, 1996; Cataglyphis viaticoides (André, 1881); and Nylanderia jaegerskioeldi (Mayr, 1904). M. abeillei was the only species recorded in all 4 seasons, while M. abeillei and C. albicans were the dominant species in summer and C. aegyptiaca and C. albicans in spring. Diversity was lowest in the autumn, with only 4 species recorded. The COI gene sequences of 5 species have been successfully deposited in the GenBank database for the first time. In total, 4 carcass decomposition stages were observed, with the longest duration in winter (13 days), the shortest in summer (11 days), and in between for both autumn and spring. Most ant species were present during both decay and dry stages, while M. abeillei, C. aegyptiaca, M. ebeninus, and C. albicans were observed in all decomposition stages. These data may indicate that ants on this coastal beach showed seasonal and geographical succession patterns that could be taken into consideration in forensic investigations.","PeriodicalId":16325,"journal":{"name":"Journal of Medical Entomology","volume":"9 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138742785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How to store a beetle larva? Comparing temporal effects of common fluid preservation methods on color, shape, and DNA quality 如何保存甲虫幼虫？比较常用液体保存方法对颜色、形状和 DNA 质量的时间影响

IF 2.1 3区农林科学

Journal of Medical Entomology Pub Date : 2023-12-12 DOI: 10.1093/jme/tjad154

Martin Novák, Pavel Jakubec, Karolina Mahlerová, Santiago Montoya-Molina, Jarin Qubaiová

{"title":"How to store a beetle larva? Comparing temporal effects of common fluid preservation methods on color, shape, and DNA quality","authors":"Martin Novák, Pavel Jakubec, Karolina Mahlerová, Santiago Montoya-Molina, Jarin Qubaiová","doi":"10.1093/jme/tjad154","DOIUrl":"https://doi.org/10.1093/jme/tjad154","url":null,"abstract":"Proper fixing and long-term preservation of entomological evidence are essential in collections and research and crucial in applied fields such as forensic entomology. Incorrectly stored samples may lose important morphological features over time, rendering molecular analyses exceedingly difficult. The most effective method for preserving soft samples such as larvae is fluid preservation. It uses a combination of a wide range of fixatives and storage fluids. However, very little comparative work has been done to determine the effects of long-term storage on sample quality in terms of color, shape, and DNA stability. Moreover, the current golden standard in forensic entomology has been tailored for age estimation of larvae of Diptera, which differ from larvae of Coleoptera in morphology and subsequently in applied methods. We compared the effects of combinations of 6 commonly used fixatives and 6 commonly used storage fluids on midsized larvae of the forensically important beetle, Necrodes littoralis (Linnaeus, 1758), in terms of color, shape, and suitability for DNA analyses over a 2-yr period. We were looking for combinations that can preserve specimens in a satisfactory state, can be used on a regular basis, do not require advanced protection or skills of the personnel, and are not toxic or too harmful to the environment. We found not only several methods that scored significantly better in the tested parameters compared with the golden standard but also several common methods that should be avoided. The effects of agents on each tested category are discussed in detail.","PeriodicalId":16325,"journal":{"name":"Journal of Medical Entomology","volume":"3 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138580369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From forests to fields: investigating Culicoides (Diptera: Ceratopogonidae) abundance and diversity in cattle pastures and adjacent woodlands 从森林到田野：调查牛牧场和邻近林地中Culicoides（双翅目：Ceratopogonidae）的丰度和多样性

IF 2.1 3区农林科学

Journal of Medical Entomology Pub Date : 2023-12-12 DOI: 10.1093/jme/tjad155

Cassandra H Steele, Emily G McDermott

{"title":"From forests to fields: investigating Culicoides (Diptera: Ceratopogonidae) abundance and diversity in cattle pastures and adjacent woodlands","authors":"Cassandra H Steele, Emily G McDermott","doi":"10.1093/jme/tjad155","DOIUrl":"https://doi.org/10.1093/jme/tjad155","url":null,"abstract":"Culicoides Latreille (Diptera: Ceratopogonidae) biting midges are hematophagous flies that feed on wild and domestic ruminants. They can transmit arboviruses, such as bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV), which circulate in the United States. Larvae occupy a range of aquatic and semiaquatic habitats, and disperse short distances from their development sites. In the southeastern United States, there are limited studies on the abundance and diversity of Culicoides in wooded and adjacent livestock pasture habitats. In this study, we characterized Culicoides diversity and abundance within these distinct habitat types. BG-Sentinel and CDC miniature suction traps baited with CO2 or UV-light were placed in wooded and pasture habitats at 2 locations on a university beef farm in Savoy, Arkansas. Traps were set once per week for 9 wk during August–October of 2021 and 2022. Fifteen species were collected during this study, and the 2 most abundant species were Culicoides haematopotus Malloch and Culicoides stellifer Coquillett. There was a significant effect of site and location on C. haematopotus collections, and a significant effect and interaction of site and trap on C. stellifer collections. In the woods, significantly more C. stellifer were collected from CDC–UV traps, while in the pasture significantly more were collected in CDC–CO2 traps. These data suggest that C. stellifer, a putative vector of BTV and EHDV in the southeast, may be traveling into the pasture to host-seek, while C. haematopotus remains primarily in wooded areas. This study reveals community differences between these habitat types and implications for Culicoides control.","PeriodicalId":16325,"journal":{"name":"Journal of Medical Entomology","volume":"16 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138580486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The distribution of triatomine (Hemiptera: Reduviidae) vectors of Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) in Illinois and Missouri: historical records and specimen submissions from community science programs 伊利诺伊州和密苏里州克氏锥虫（Kinetoplastida: Trypanosomatidae）的三足类（半翅目：Reduviidae）载体的分布：历史记录和社区科学项目提交的标本

IF 2.1 3区农林科学

Journal of Medical Entomology Pub Date : 2023-12-09 DOI: 10.1093/jme/tjad124

Ellen M Santos, Catherine D Santanello, Rachel Curtis-Robles, Keswick Killets, Gena Lawrence, Jet Sevenshadows, Meredith J Mahoney, Molly Baker, Sarah A Hamer

{"title":"The distribution of triatomine (Hemiptera: Reduviidae) vectors of Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) in Illinois and Missouri: historical records and specimen submissions from community science programs","authors":"Ellen M Santos, Catherine D Santanello, Rachel Curtis-Robles, Keswick Killets, Gena Lawrence, Jet Sevenshadows, Meredith J Mahoney, Molly Baker, Sarah A Hamer","doi":"10.1093/jme/tjad124","DOIUrl":"https://doi.org/10.1093/jme/tjad124","url":null,"abstract":"Triatomine species (kissing bugs) infected with Trypanosoma cruzi are found across the southern United States. The northern limits of Trypanosoma cruzi infected kissing bugs are less understood. The objective of this work was to describe the locations of kissing bugs from Illinois and Missouri based on historical records, submissions to Texas A&M University’s (TAMU) Kissing Bug Community Science Program and the Centers for Disease Control and Prevention (CDC), and records from online platforms (iNaturalist, BugGuide, and GBIF) up to and including 2022. A total of 228 records were discovered, including 186 from historical or observation platforms and 42 specimens submitted to TAMU or CDC. Species included Triatoma sanguisuga (221 total records, 9 nymphs) and Triatoma lecticularia (7 records). Notably, nearly all (24/26) records submitted to TAMU were collected indoors. Twelve of the 30 (40%) specimens tested were positive for the presence of T. cruzi, including parasite discrete taxonomic units TcI and TcIV. One triatomine sample had been found in a bed feeding on the submitter; this bug was positive for T. cruzi and had evidence of human blood in its gut. Records suggest a ubiquitous distribution in Missouri and potentially to the northernmost border in Illinois. Further investigations into triatomine distribution and infection status are needed within states assumed to be northern limits in order to create public health and veterinary health messaging and baseline distributional maps from which to measure future range shifts in relation to a changing climate.","PeriodicalId":16325,"journal":{"name":"Journal of Medical Entomology","volume":"15 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138559781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fir (Abies balsamea) (Pinales: Pinaceae) needle essential oil enhances the knockdown activity of select insecticides. 冷杉(Abies balsamea) (Pinales: Pinales: Pinaceae)针叶精油可增强部分杀虫剂的抑菌活性

IF 2.1 3区农林科学

Journal of Medical Entomology Pub Date : 2023-11-14 DOI: 10.1093/jme/tjad101

Edmund J Norris, Jeffrey R Bloomquist

{"title":"Fir (Abies balsamea) (Pinales: Pinaceae) needle essential oil enhances the knockdown activity of select insecticides.","authors":"Edmund J Norris, Jeffrey R Bloomquist","doi":"10.1093/jme/tjad101","DOIUrl":"10.1093/jme/tjad101","url":null,"abstract":"Because of the increased interest in plant essential oils (PEO) for both home pest control and personal bite protection, the ability of fir needle (Abies balsamea) oil to synergize the 1-h knockdown and 24-h toxicity of 9 different synthetic insecticides was evaluated. Fir needle oil strongly synergized knockdown of the neonicotinoids, clothianidin, and thiamethoxam (between 16- and 24-fold), as well as natural pyrethrins (12-fold), but had less effect with organophosphates and fipronil. For 24-h mortality, only pirimiphos-methyl was strongly synergized by fir needle oil pretreatment (18-fold). Chemical analysis and testing identified delta-3-carene is the most bioactive constituent, producing synergism similar to that of the whole oil. In fact, this constituent synergized the 24-h mortality of clothianidin to a higher degree than fir needle oil itself (4.9-fold vs. 2.4-fold). Synergism is unlikely to be mediated by effects on the nervous system, as fir needle oil caused no change in mosquito central nervous system firing at 100 ppm and did not synergize an inactive concentration of natural pyrethrins (10 nM). To better understand fir needle oil effects, we evaluated the ability of pretreatment with this oil to impact Aedes aegypti monooxygenase degradation of a model substrate, 7-ethoxycoumarin. Interestingly, both fir needle oil and delta-3-carene caused a significant increase in metabolic degradation of 7-ethoxycoumarin, perhaps indicating they upregulate oxidative metabolic processes. Such an action would explain why fir needle oil enhances knockdown, but not 24-h mortality for most of the insecticides studied here, whereas increased bioactivation would explain the synergism of pirimiphos-methyl toxicity.","PeriodicalId":16325,"journal":{"name":"Journal of Medical Entomology","volume":" ","pages":"1350-1356"},"PeriodicalIF":2.1,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45303576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Off-host survival of Dermacentor variabilis (Acari: Ixodidae) adults near their northern distributional limit in Saskatchewan, Canada. 加拿大萨斯喀彻温省变异性革螨(蜱螨:伊蚊科)成虫在其北部分布边界附近的离宿主生存。

IF 2.1 3区农林科学

Journal of Medical Entomology Pub Date : 2023-11-14 DOI: 10.1093/jme/tjad106

Matthew E M Yunik, Chulantha P Diyes, Neil B Chilton

{"title":"Off-host survival of Dermacentor variabilis (Acari: Ixodidae) adults near their northern distributional limit in Saskatchewan, Canada.","authors":"Matthew E M Yunik, Chulantha P Diyes, Neil B Chilton","doi":"10.1093/jme/tjad106","DOIUrl":"10.1093/jme/tjad106","url":null,"abstract":"Dermacentor variabilis (Say) is expanding its range northwards in Saskatchewan and Manitoba (Canada). Knowledge of the relative ability of different life cycle stages to survive off-host over winter is important for assessing the potential for further range expansion and risk of pathogen transmission. In the present study, the off-host survival of D. variabilis adults was determined in outdoor terraria within a field plot close to Lizard Lake Community Pasture (LLCP) situated near the northern distributional limit in Saskatchewan. Only a small proportion (7%) of adult ticks in this relatively recently established population survived from mid-summer to late winter. Off-host survival had declined further to <4% by mid-spring. A significantly greater proportion of female ticks (6%) survived from mid-summer to mid-spring than did male ticks (1%). The supercooling points (SCPs), the temperature below 0 °C when body fluids spontaneously freeze, of the 17 ticks that survived through to mid-spring did not differ significantly from the SCPs of questing ticks in the previous summer, suggesting adult D. variabilis do not undergo cold hardening during winter diapause. The off-host survival of D. variabilis adults near LLCP was also much lower than that previously reported for adults from Sandy Hook in Manitoba, a population that has been established for decades. This finding suggests that there is geographical variation in the ability of D. variabilis in southern Canada to survive from mid-summer to the subsequent spring. However, the ecological and genetic factors influencing the survival of northern populations of D. variabilis requires further investigation.","PeriodicalId":16325,"journal":{"name":"Journal of Medical Entomology","volume":" ","pages":"1288-1296"},"PeriodicalIF":2.1,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10015118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histamine excretion in common indoor and hematophagous arthropods. 普通室内和食血节肢动物的组胺排泄。

IF 2.1 3区农林科学

Journal of Medical Entomology Pub Date : 2023-11-14 DOI: 10.1093/jme/tjad103

Simona Principato, Alvaro Romero, Chow-Yang Lee, Kathleen Campbell, Dong-Hwan Choe, Coby Schal, Zachary DeVries

{"title":"Histamine excretion in common indoor and hematophagous arthropods.","authors":"Simona Principato, Alvaro Romero, Chow-Yang Lee, Kathleen Campbell, Dong-Hwan Choe, Coby Schal, Zachary DeVries","doi":"10.1093/jme/tjad103","DOIUrl":"10.1093/jme/tjad103","url":null,"abstract":"Histamine is a biogenic amine that regulates multiple physiological functions in diverse organisms, specifically playing a central role in the mammalian immune response. The common bed bug, Cimex lectularius L. (Hemiptera: Cimicidae), excretes histamine in large amounts in its feces as a component of its aggregation pheromone, which contaminates homes. The potential health risks associated with the presence of indoor histamine are unclear, but to predict future exposure risks, it is critical that we understand if other arthropods excrete histamine in any discernible phylogenetic pattern. In the present study, we evaluated histamine excretion by various arthropods; specifically those commonly found in large numbers indoors, other hematophagous species, and other species in the order Hemiptera. To evaluate arthropods for histamine excretion, rearing containers for each arthropod were swabbed and/or the harborage substrates were collected. Samples were then analyzed for the presence of histamine using gas chromatography-mass spectrometry. For those arthropods where histamine was present above the method detection limit, total histamine excretion was quantified over a period of 2 wk. Our results indicate that histamine excretion is limited to hematophagous hemipterans (bed bugs, bat bugs, tropical bed bugs, and kissing bugs), suggesting that indoor histamine contamination in the United States can be primarily linked to bed bugs.","PeriodicalId":16325,"journal":{"name":"Journal of Medical Entomology","volume":" ","pages":"1269-1277"},"PeriodicalIF":2.1,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10645371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10068282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acquisition of Rickettsia rickettsii (Rickettsiales: Rickettsiaceae) by Haemaphysalis longicornis (Acari: Ixodidae) through co-feeding with infected Dermacentor variabilis (Acari: Ixodidae) in the laboratory. 在实验室中，长角血蜱(蜱螨科)通过与受感染的变异性革螨(蜱螨科)共食获得立克次体(立克次体亚纲:立克次体科)

IF 2.1 3区农林科学

Journal of Medical Entomology Pub Date : 2023-11-14 DOI: 10.1093/jme/tjad107

Britny Johnson, Alyssa N Snellgrove, Sara E McBride, Kris Hartzer, Michael L Levin, William L Nicholson

{"title":"Acquisition of Rickettsia rickettsii (Rickettsiales: Rickettsiaceae) by Haemaphysalis longicornis (Acari: Ixodidae) through co-feeding with infected Dermacentor variabilis (Acari: Ixodidae) in the laboratory.","authors":"Britny Johnson, Alyssa N Snellgrove, Sara E McBride, Kris Hartzer, Michael L Levin, William L Nicholson","doi":"10.1093/jme/tjad107","DOIUrl":"10.1093/jme/tjad107","url":null,"abstract":"Haemaphysalis longicornis (Neumann) is an invasive ixodid tick originating from eastern Asia which recently has become established in the United States. In its native range, this tick can transmit several pathogens to animals and humans, but little is known about its ability to acquire and transmit pathogens native to the United States. Geographic overlap with ticks such as Dermacentor variabilis (Say), a known vector of Rickettsia rickettsii, makes investigation into the interactions between H. longicornis and D. variabilis of interest to the public health community. Previous studies have shown that H. longicornis can serve as a competent vector of R. rickettsii under laboratory settings, but there is little information on its ability to acquire this pathogen via other biologically relevant routes, such as co-feeding. Here, we assess the ability of H. longicornis nymphs to acquire R. rickettsii through co-feeding with infected D. variabilis adults on a vertebrate animal model under laboratory conditions. The median infection prevalence in engorged H. longicornis nymphs across 8 cohorts was 0% with an interquartile range (IQR) of 4.13%. Following transstadial transmission, the median infection prevalence in flat females was 0.7% (IQR = 2.4%). Our results show that co-feeding transmission occurs at low levels in the laboratory between these 2 species. However, based on the relatively low transmission rates, this may not be a likely mechanism of R. rickettsii introduction to H. longicornis.","PeriodicalId":16325,"journal":{"name":"Journal of Medical Entomology","volume":" ","pages":"1380-1387"},"PeriodicalIF":2.1,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43556258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative bioactivity of S-methoprene and novel S-methobutene against mosquitoes (Diptera: Culicidae). 甲氧丁烯与新型甲氧丁烯对蚊虫的生物活性比较(双翅目:库蚊科)。

IF 2.1 3区农林科学

Journal of Medical Entomology Pub Date : 2023-11-14 DOI: 10.1093/jme/tjad110

Tianyun Su, Jin-Sheng Yu, Ying Zhang, Xuhong Qian, Heng Su

{"title":"Comparative bioactivity of S-methoprene and novel S-methobutene against mosquitoes (Diptera: Culicidae).","authors":"Tianyun Su, Jin-Sheng Yu, Ying Zhang, Xuhong Qian, Heng Su","doi":"10.1093/jme/tjad110","DOIUrl":"10.1093/jme/tjad110","url":null,"abstract":"Mosquitoes and mosquito-borne illnesses significantly impact public health and human well-being. To address this concern, environmentally compatible larvicides have become a critical component of integrated mosquito management. However, the number of available larvicides is at a historical low. Currently, larvicides that harness microbials and insect growth regulators account for most products. Screening of new active ingredients (AIs) or improvement of existing AIs is thus necessary to augment the capacity for mosquito control. S-methoprene possesses a similar molecular structure and identical function to mosquito juvenile hormone and has been one of the main targets for research and development. The efficacy and safety of S-methoprene have been well documented since the late 1960s, and numerous products have been commercialized to combat pests of economic importance. However, S-methoprene is vulnerable to environmental factors that lead to its degradation, which has created challenges in formulation development, particularly where extended efficacy is desired. A derivative of S-methoprene, namely S-methobutene, with molecular modification has become available. This derivative has demonstrated an enhanced activity of inhibition of emergence (IE) against species across the Aedes, Anopheles, and Culex genera at IE10, IE50, and IE90. Furthermore, S-methobutene consistently outperformed S-methoprene during a 120-day aging process against the southern house mosquito Cx. quinquefasciatus, where the IE% in S-methobutene was significantly higher than that in S-methoprene on most aging intervals. The former had significantly longer residual activity than the latter. The potential of S-methobutene for further development and application is discussed in consideration of its enhanced activity and stability.","PeriodicalId":16325,"journal":{"name":"Journal of Medical Entomology","volume":" ","pages":"1357-1363"},"PeriodicalIF":2.1,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10066395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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