Journal of lipid mediators最新文献_第8页

PGE2 in the perinatal brain: local synthesis and transfer across the blood brain barrier. 围产期脑中的PGE2:局部合成并通过血脑屏障转移。

Journal of lipid mediators Pub Date : 1993-03-01

S A Jones, S L Adamson, D Engelberts, I Bishai, J Norton, F Coceani

引用次数: 0

Expression of 5-lipoxygenase and biosynthesis of leukotriene B4 in human monomorphonuclear leukocytes. 5-脂氧合酶在人单核白细胞中的表达及白三烯B4的生物合成。

Journal of lipid mediators Pub Date : 1993-03-01

H E Claesson, P J Jakobsson, D Steinhilber, B Odlander, B Samuelsson

引用次数: 0

Differential regulation of cytokine and cytokine receptor genes by PAF, LTB4 and PGE2. PAF、LTB4和PGE2对细胞因子和细胞因子受体基因的差异调控。

Journal of lipid mediators Pub Date : 1993-03-01

M Rola-Pleszczynski, M Thivierge, N Gagnon, C Lacasse, J Stankova

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Prostaglandins as modulators rather than mediators of inflammation. 前列腺素是炎症的调节剂而不是介质。

Journal of lipid mediators Pub Date : 1993-03-01

G Weissmann

引用次数: 0

Enhancement by GM-CSF of agonist-induced 5-lipoxygenase activation in human neutrophils involves protein synthesis and gene transcription. GM-CSF增强激动剂诱导的人中性粒细胞5-脂氧合酶激活涉及蛋白质合成和基因转录。

Journal of lipid mediators Pub Date : 1993-03-01

P P McDonald, M Pouliot, P Borgeat

引用次数: 0

Leukotriene A4 hydrolase: structural and functional properties of the active center. 白三烯A4水解酶:活性中心的结构和功能性质。

Journal of lipid mediators Pub Date : 1993-03-01

J Z Haeggström, A Wetterholm, J F Medina, B Samuelsson

{"title":"Leukotriene A4 hydrolase: structural and functional properties of the active center.","authors":"J Z Haeggström, A Wetterholm, J F Medina, B Samuelsson","doi":"","DOIUrl":"","url":null,"abstract":"Leukotriene (LT) A4 hydrolase (EC 3.3.2.6) is a bifunctional zinc metalloenzyme that possesses both an epoxide hydrolase activity, i.e., the well-known conversion of LTA4 into the proinflammatory substance LTB4, and a recently discovered peptidase activity. We have employed biochemical/kinetic analyses of native enzyme as well as site directed mutagenesis towards a recombinant enzyme to explore structural and functional properties of the enzyme active center. Thus, we have found that the peptidase activity is selectively stimulated by chloride ions, in a manner that suggests the presence of an anion binding site. Furthermore, a number of mutated enzymes have been constructed, expressed in E. coli, and purified to homogeneity to allow enzyme activity determinations and zinc analyses. The catalytic properties and zinc contents of these mutated enzymes establish the three zinc binding ligands of the protein and identify Glu-296 as a catalytic amino acid, directly involved in the peptidase, but not in the epoxide hydrolase reaction. In conclusion, our data provide strong evidence that the two catalytic activities of LTA4 hydrolase are exerted via non-identical but overlapping active sites.","PeriodicalId":16323,"journal":{"name":"Journal of lipid mediators","volume":"6 1-3","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19343766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical and molecular biological approaches to two types of arachidonate 12-lipoxygenase. 两类花生四烯酸12-脂加氧酶的生化和分子生物学研究。

Journal of lipid mediators Pub Date : 1993-03-01

S Yamamoto, T Yoshimoto, Y Takahashi, H Suzuki, T Arakawa, K Kishimoto, T Hada, T Oshima, J Murakami, Y Yamamoto

引用次数: 0

Human 15-lipoxygenase: induction by interleukin-4 and insights into positional specificity. 人15-脂氧合酶:由白细胞介素-4诱导和位置特异性的见解。

Journal of lipid mediators Pub Date : 1993-03-01

E Sigal, D L Sloane, D J Conrad

{"title":"Human 15-lipoxygenase: induction by interleukin-4 and insights into positional specificity.","authors":"E Sigal, D L Sloane, D J Conrad","doi":"","DOIUrl":"","url":null,"abstract":"Arachidonate 15-lipoxygenase (15-lipoxygenase) is a lipid-peroxidizing enzyme associated with specific inflammatory cells seen in asthma and atherosclerosis. In atherosclerosis, 15-lipoxygenase is induced in the macrophages of human and rabbit lesions and has been implicated in foam cell formation. In human lung, 15-lipoxygenase is preferentially expressed in airway epithelial cells and eosinophils. Our studies have focused both on the regulation of expression and on the structure-function relationships of the enzyme. To determine factors that could regulate expression, peripheral blood monocytes were purified and cultured with combinations of 18 factors. Only interleukin-4 (60 pM) induced 15-lipoxygenase mRNA, protein and enzymatic activity. Interferon-gamma (100 pM) inhibited the interleukin-4 dependent induction of 15-lipoxygenase. Results with cultured human airway cells were similar. These data suggest that expression of 15-lipoxygenase is regulated by interleukin-4, and that 15-lipoxygenase is a potential downstream effector molecule for this potent cytokine. In parallel studies, we have investigated determinants of positional specificity using site-directed mutagenesis and bacterial expression of human 15-lipoxygenase. Hypotheses for mutagenesis were derived from an analysis of conserved differences among multiple lipoxygenase sequences. Switching four amino acids in 15-lipoxygenase to their counterparts in 12-lipoxygenase resulted in a variant enzyme that produced equal 12- and 15-lipoxygenation. Further analysis has identified two amino acids that completely control the positional specificity of 15-lipoxygenase. These data have led to a preliminary model of the enzyme's active site region.","PeriodicalId":16323,"journal":{"name":"Journal of lipid mediators","volume":"6 1-3","pages":"75-88"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19344157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleukin 1 and interleukin 6 inhibition of mesangial cell proliferation: role of PGE2. 白细胞介素1和白细胞介素6抑制系膜细胞增殖:PGE2的作用。

Journal of lipid mediators Pub Date : 1993-03-01

D G Matsell, L W Gaber, E Sehic, K U Malik

引用次数: 0

Effect of aging on renal cytochrome P450-dependent arachidonic acid metabolism in Dahl rats. 衰老对Dahl大鼠肾细胞色素p450依赖性花生四烯酸代谢的影响。

Journal of lipid mediators Pub Date : 1993-03-01

K Omata, E Tsutsumi, H L Sheu, Y Utsumi, K Abe

{"title":"Effect of aging on renal cytochrome P450-dependent arachidonic acid metabolism in Dahl rats.","authors":"K Omata, E Tsutsumi, H L Sheu, Y Utsumi, K Abe","doi":"","DOIUrl":"","url":null,"abstract":"We investigated the age-related changes of renal cytochrome P450-dependent arachidonic acid metabolism in 3-, 5-, 7-, 9-, 11-, 13- and 20-week-old male Dahl salt-sensitive (DS) and -resistant (DR) rats on a low sodium diet (0.3% NaCl). NADPH-dependent arachidonic acid metabolism was separated and measured by a radio-HPLC system. The formation of 19-hydroxyeicosatetraenoic acid (HETE), 20 HETE, 1,20-dioic acid, epoxyeicosatrienoic acids (EETs), and dihydroxyeicosatrienoic acid (DHET) was age dependent in both DS and DR rats. omega-Hydroxylase (20-HETE and 1,20-dioic acid formation) and (omega-1)-hydroxylase (19-HETE formation) were increased from 3 to 5 weeks age, then decreased with aging in DR rats. Whilst omega/(omega-1)-hydroxylase activities were increased from 3- to 9-week-old rats, they decreased with aging in DS rats. omega/(omega-1)-Hydroxylase activities were higher in 3-5-week-old DR than DS rats. Epoxygenase activities (EETs and DHET formations) were highest in 3-week-old DS and DR rats, and showed no significant differences between two strains of rats at any ages tested. Renal cytochrome P450-dependent arachidonic acid metabolites have a wide and contrasting spectrum of biological and renal effects, and their relative rates of production may influence not only renal hemodynamics but also pro- and antihypertensive mechanisms of hypertension in Dahl rats.","PeriodicalId":16323,"journal":{"name":"Journal of lipid mediators","volume":"6 1-3","pages":"369-73"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19344977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0