Journal of Laboratory and Clinical Medicine最新文献_第4页

The peripheral blood transcriptome dynamically reflects system wide biology: a potential diagnostic tool 外周血转录组动态反映全系统生物学:一种潜在的诊断工具

Journal of Laboratory and Clinical Medicine Pub Date : 2006-03-01 DOI: 10.1016/j.lab.2005.10.005

Choong-Chin Liew , Jun Ma , Hong-Chang Tang , Run Zheng , Adam A. Dempsey

{"title":"The peripheral blood transcriptome dynamically reflects system wide biology: a potential diagnostic tool","authors":"Choong-Chin Liew , Jun Ma , Hong-Chang Tang , Run Zheng , Adam A. Dempsey","doi":"10.1016/j.lab.2005.10.005","DOIUrl":"10.1016/j.lab.2005.10.005","url":null,"abstract":"<div><p>In our genome-wide survey of gene expression in human peripheral blood cells using both an expressed sequence tag (EST) and a microarray hybridization approach, we identified the expression of a large proportion (approximately 80%) of the genes encoded in the human genome. Comparison of the peripheral blood transcriptome with genes expressed in nine different human tissue types revealed that expression of over 80% was shared with any given tissue. We also sought to determine whether those gene transcripts undetected by these methods were also expressed in peripheral blood cells. Using reverse-transcriptase-polymerase chain reaction, we detected additional tissue-specific gene transcripts including beta-myosin heavy chain (heart specific) and insulin (specific to pancreatic islet beta cells), in circulating blood cells. Arguably, the detection of low levels of tissue-specific transcripts could be considered products of “illegitimate” transcription; however, our study also demonstrates that environmental conditions affect the transcriptional regulation of insulin in the peripheral blood. We thus hypothesize that blood cells can act as sentinels of disease and that we could capitalize on this property of blood for the diagnosis/prognosis of disease (the “Sentinel Principle”). Peripheral blood is an ideal surrogate tissue as it is readily obtainable, provides a large biosensor pool in the form of gene transcripts, and response to changes in the macro- and micro-environments is detectable as alterations in the levels of these gene transcripts.</p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 3","pages":"Pages 126-132"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2005.10.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25877138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 588

About the cover illustration: Saint Mary’s of Bethlehem (“Bedlam”) 关于封面插图:伯利恒圣玛丽教堂(“疯人院”)

Journal of Laboratory and Clinical Medicine Pub Date : 2006-03-01 DOI: 10.1016/J.LAB.2006.02.002

D. Hammerschmidt

引用次数: 0

Molecular targeted therapy: A strategy of disillusions or optimism? 分子靶向治疗:是一种幻灭还是乐观的策略?

Journal of Laboratory and Clinical Medicine Pub Date : 2006-03-01 DOI: 10.1016/j.lab.2005.11.005

Sándor Eckhardt

引用次数: 10

This month in J Lab Clin Med 本月在J Lab临床医学杂志上

Journal of Laboratory and Clinical Medicine Pub Date : 2006-03-01 DOI: 10.1016/j.lab.2006.02.003

Dale E. Hammerschmidt MD (Editor-in-Chief)

引用次数: 0

The anion gap (AG): studies in the nephrotic syndrome and diabetic ketoacidosis (DKA) 阴离子间隙(AG):肾病综合征和糖尿病酮症酸中毒(DKA)的研究

Journal of Laboratory and Clinical Medicine Pub Date : 2006-03-01 DOI: 10.1016/j.lab.2005.10.004

Howard E. Corey

引用次数: 21

Long-term effect of Heme oxygenase (HO)-1 induction in glomerular immune injury 血红素加氧酶(HO)-1诱导对肾小球免疫损伤的远期影响

Journal of Laboratory and Clinical Medicine Pub Date : 2006-03-01 DOI: 10.1016/j.lab.2005.11.009

Prasun K. Datta , Pu Duann , Elias A. Lianos

{"title":"Long-term effect of Heme oxygenase (HO)-1 induction in glomerular immune injury","authors":"Prasun K. Datta , Pu Duann , Elias A. Lianos","doi":"10.1016/j.lab.2005.11.009","DOIUrl":"10.1016/j.lab.2005.11.009","url":null,"abstract":"<div><p>In a rat model of macrophage-dependent glomerular immune injury induced by administration of antibody against the glomerular basement membrane (anti-GBM), the authors assessed the anti-proteinuric effect of Heme Oxygenase-1 (HO-1) induction. Rats received anti-GBM antibody alone, anti-GBM antibody and treatment with the HO-1 inducer, hemin, or non-immune serum (controls). Urine protein, creatinine, and nitrite/nitrate excretion were measured on days 5, 7, and 14 after administration of the anti-GBM antibody. In hemin-treated animals with anti-GBM antibody-induced immune injury, HO-1 immunolocalized in macrophages infiltrating glomeruli and in tubular epithelial cells. In these animals, proteinuria was decreased. There was also a decrease in blood urea nitrogen (BUN) levels without a change in serum creatinine or systemic blood pressure. The observations establish the anti-proteinuric effect of hemin induction. This effect could be mechanistically linked to blunting of the ability of infiltrating macrophages to cause injury or to changes in tubular handling of filtered protein.</p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 3","pages":"Pages 150-155"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2005.11.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25876385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Rapid, accurate, and sensitive fatty acid ethyl ester determination by gas chromatography-mass spectrometry 气相色谱-质谱联用法快速、准确、灵敏地测定脂肪酸乙酯

Journal of Laboratory and Clinical Medicine Pub Date : 2006-03-01 DOI: 10.1016/j.lab.2005.11.006

Clark C. Kulig , Thomas P. Beresford , Gregory T. Everson

{"title":"Rapid, accurate, and sensitive fatty acid ethyl ester determination by gas chromatography-mass spectrometry","authors":"Clark C. Kulig , Thomas P. Beresford , Gregory T. Everson","doi":"10.1016/j.lab.2005.11.006","DOIUrl":"10.1016/j.lab.2005.11.006","url":null,"abstract":"<div><p>Background: Fatty acid ethyl esters (FAEEs) are useful markers of ongoing alcohol use and may be associated with alcohol-induced damage to the liver and pancreas. In this article, we describe a novel method for rapid determination of the three major FAEEs found in human plasma. Methods: Internal standard, ethyl heptadecanoate, was added to plasma samples, and FAEEs were isolated by acetone precipitation, hexane lipid extraction, and amino-propyl silica solid phase extraction. FAEEs were quantitated by gas chromatography-mass spectrometry (GC-MS) using a nonpolar dimethylpolysiloxane column. The accuracy, precision, specificity, and sensitivity of the assay were defined from plasma samples from recently drinking and abstinent persons, with and without the addition of FAEEs. Results: Individual FAEE peaks demonstrated excellent resolution. Instrument time was reduced by more than 60%. The lower limit of detection was 5 to 10 nM, and the lower limit of quantitation for each FAEE was 60 nM (for 22 samples with known concentration 60 nM, × ±SD: 61 ± 5.7, 57 ± 5.7, and 57 ± 5.9 nM, for ethyl palmitate, ethyl oleate, and ethyl stearate, respectively). Instrument precision (coefficient of variance, CV) for these three FAEEs was 0.3%, 0.4%, and 0.7%, respectively. Intra-assay precision (CV) for total FAEEs was less than 7%. FAEEs were absent in 49 samples from abstinent persons. FAEEs were detected in all 76 samples with associated positive blood alcohol levels. Conclusions: Our method of FAEE analysis is rapid and potentially useful in research and clinical studies. FAEE determination using this method is precise, accurate, sensitive, and specific and deserves broader application.</p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 3","pages":"Pages 133-138"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2005.11.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25877139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Comparison of a new office-based stool immunoassay and 13C-UBT in the diagnosis of current Helicobacter pylori infection 新型办公室粪便免疫测定法与13C-UBT诊断幽门螺杆菌感染的比较

Journal of Laboratory and Clinical Medicine Pub Date : 2006-03-01 DOI: 10.1016/j.lab.2005.11.007

I.-Chen Wu , Sheng-Wen Wang , Yuan-Chieh Yang , Fang-Jung Yu , Chao-Hung Kuo , Chieh-Han Chuang , Yi-Chen Lee , Hung-Lung Ke , Fu-Chen Kuo , Lin-Li Chang , Wen-Ming Wang , Chang-Ming Jan , Deng-Chyang Wu

{"title":"Comparison of a new office-based stool immunoassay and 13C-UBT in the diagnosis of current Helicobacter pylori infection","authors":"I.-Chen Wu , Sheng-Wen Wang , Yuan-Chieh Yang , Fang-Jung Yu , Chao-Hung Kuo , Chieh-Han Chuang , Yi-Chen Lee , Hung-Lung Ke , Fu-Chen Kuo , Lin-Li Chang , Wen-Ming Wang , Chang-Ming Jan , Deng-Chyang Wu","doi":"10.1016/j.lab.2005.11.007","DOIUrl":"10.1016/j.lab.2005.11.007","url":null,"abstract":"<div><p>Noninvasive methods to diagnose the infection status of <em>Helicobacter pylori</em> were a new developed trend. In this study, the authors sought to investigate the difference between a new office-based stool immunoassay (ImmunoCard STAT! HpSA) and <sup>13</sup>C-Urea Breath Test (<sup>13</sup>C-UBT). We studied 254 dyspeptic patients (159 men, 95 women; mean age = 52.8 ± 14.3 years, range: 19–89 years). All of them underwent gastroendoscopy, <sup>13</sup>C-UBT test, and delivered stool samples within 3 days after endoscopy for the ImmunoCard STAT! HpSA test. The exclusion criteria were those who (1) had received previous anti-<em>Hp</em> treatment, proton pump inhibitor, antibiotics, or bismuth within 1 month of endoscopic examination; (2) had bleeding peptic ulcers; (3) had previously undergone gastric surgery; (4) had long-term use of corticosteroid or immunosuppressant drugs; (5) were pregnant or lactating; and (6) had incomplete data. <em>Hp</em> infection was considered positive when either culture was positive, or both histology and rapid urea test were positive. Those patients were classified as pre- and post-<em>Hp</em> treatment groups. Those in the post-treatment group were patients who received <em>Hp</em> eradication therapy at our hospital more than 2 months ago. The overall sensitivity, specificity, and positive and negative predictive values of <sup>13</sup>C-UBT and ImmunoCard STAT! HpSA were 96.3%, 87.6%, 85.4%, 96.9%, and 95.4%, 83.4%, 81.3%, 96.0%, respectively. The sensitivity, specificity, and accuracy of both tests are comparable in the pre- and post- treatment groups. The advantages of ImmunoCard STAT! HpSA over a breath test are that it is cheaper, more time-saving, and can be used in-office.</p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 3","pages":"Pages 145-149"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2005.11.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25876384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Pseudohyperkalemia in serum: the phenomenon and its clinical magnitude 血清假性高钾血症:现象及其临床意义

Journal of Laboratory and Clinical Medicine Pub Date : 2006-03-01 DOI: 10.1016/j.lab.2005.11.008

Nikolaos Sevastos , George Theodossiades , Stamatis Efstathiou , George V. Papatheodoridis , Emanuel Manesis , Athanasios J. Archimandritis

{"title":"Pseudohyperkalemia in serum: the phenomenon and its clinical magnitude","authors":"Nikolaos Sevastos , George Theodossiades , Stamatis Efstathiou , George V. Papatheodoridis , Emanuel Manesis , Athanasios J. Archimandritis","doi":"10.1016/j.lab.2005.11.008","DOIUrl":"10.1016/j.lab.2005.11.008","url":null,"abstract":"<div><p>We investigated in detail the difference between serum and plasma potassium levels in patients with several conditions associated with pseudohyperkalemia. In total, 435 patients with either thrombocytoses, erythrocytoses, leucocytoses, or a mixed-type disorder and 30 healthy controls were included. In each case, the index Dk [serum potassium minus plasma potassium] and the index Dk100 (Dk × 100,000/platelets), which indicates the Dk value that corresponds to platelets of 100,000/mm<sup>3</sup>, were estimated. Median Dk was significantly higher in the groups with platelet, erythrocyte, or mixed-type disorders than in the controls (<em>P</em> = 0.001). Among these groups, Dk values were significantly higher in patients with thrombocytosis or mixed-type disorders compared with those with erythrocytosis (<em>P</em> < 0.001, for both). Furthermore, no significant difference was observed in Dk values between controls and patients with white blood cell disorders (<em>P</em> = 0.74). Dk values did not exceed 2.61 mmol/L, whereas Dk100 values were inversely related to platelet counts (<em>r</em> = −0.351, <em>P</em> < 0.01). In conclusion, pseudohyperkalemia is mainly present in patients with thrombocytosis or mixed-type disorders, probably as a result of the degranulation of platelets, which offers a potassium load to the surrounding plasma at the time of clot formation <em>in vitro</em>. However, the degree of pseudohyperkalemia does not increase proportionally with the increase of platelet counts, which may be associated with transfer of part of potassium load from the plasma back into red and white blood cells.</p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 3","pages":"Pages 139-144"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2005.11.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25877140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 53

About the cover illustration: Saint Mary’s of Bethlehem (“Bedlam”) 关于封面插图:伯利恒圣玛丽教堂(“疯人院”)

Journal of Laboratory and Clinical Medicine Pub Date : 2006-03-01 DOI: 10.1016/j.lab.2006.02.002

Dale E. Hammerschmidt MD (Editor-in-Chief)

引用次数: 0