Journal of Laboratory and Clinical Medicine最新文献

{"title":"This month in J Lab Clin Med","authors":"Dale E. Hammerschmidt MD (Editor-in-Chief)","doi":"10.1016/j.lab.2006.04.005","DOIUrl":"https://doi.org/10.1016/j.lab.2006.04.005","url":null,"abstract":"","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 5","pages":"Pages 207-210"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2006.04.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordinate activation of intracellular signaling pathways by insulin-like growth factor-1 and platelet-derived growth factor in rat hepatic stellate cells","authors":"Kim R. Bridle ,&nbsp;Lin Li,&nbsp;Rosemary O’Neill,&nbsp;Robert S. Britton,&nbsp;Bruce R. Bacon","doi":"10.1016/j.lab.2005.12.009","DOIUrl":"10.1016/j.lab.2005.12.009","url":null,"abstract":"<div><p>Proliferation of activated hepatic stellate cells (HSC) is an important event in the development of hepatic fibrosis. Insulin-like growth factor-1 (IGF-1) has been shown to be mitogenic for HSC, but the intracellular signaling pathways involved have not been fully characterized. Thus, the aims of the current study were to examine the roles of the extracellular signal-regulated kinase (ERK), phosphatidylinositol 3-kinase<span> (PI3-K) and p70-S6 kinase (p70-S6-K) signaling pathways in IGF-1- and platelet-derived growth factor (PDGF)-induced mitogenic signaling of HSC and to examine the potential crosstalk between these pathways. Both IGF-1 and PDGF increased ERK, PI3-K and p70-S6-K activity. When evaluating potential crosstalk between these signaling pathways, we observed that PI3-K is required for p70-S6-K activation by IGF-1 and PDGF, and is partially responsible for PDGF-induced ERK activation. PDGF and IGF-1 also increased the levels of cyclin D1 and phospho-glycogen synthase kinase-3β. Coordinate activation of ERK, PI3-K and p70-S6-K is important for perpetuating the activated state of HSC during fibrogenesis.</span></p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 5","pages":"Pages 234-241"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2005.12.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26023342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential gene expression identifies subgroups of renal cell carcinoma","authors":"Keith M. Skubitz ,&nbsp;Wolfgang Zimmerman ,&nbsp;Robert Kammerer ,&nbsp;Stefan Pambuccian ,&nbsp;Amy P.N. Skubitz","doi":"10.1016/j.lab.2006.04.001","DOIUrl":"10.1016/j.lab.2006.04.001","url":null,"abstract":"<div><p>Clear cell carcinoma of the kidney, the most common subtype of renal cell cancer, displays different biological behavior in different patients. This heterogeneity cannot be recognized by light microscopy. In this study, gene expression in 16 clear cell renal cell carcinoma samples and 17 non-malignant tissue types comprising 539 samples was determined using oligonucleotide microarrays representing approximately 40,000 known genes and ESTs. Differences in gene expression were quantified as the fold change in gene expression between the various sets of samples. A set of genes was identified that was overexpressed in the renal cell carcinoma samples compared with the normal kidney samples. Principle component analysis of the set of renal cell carcinomas using this set of genes overexpressed in renal cell cancer revealed the existence of 2 major subgroups among the renal carcinomas. A series of principle component analyses of the set of renal cell carcinomas using different gene sets composed of genes involved in different metabolic pathways also revealed the same 2 major subgroups of the renal cell cancers. Eisen clustering using the same genes also revealed the same 2 major renal cell cancer subsets. Review of the pathology suggested that these 2 subgroups differed in pathologic grade. Genes differentially expressed between the 2 renal cell cancer subsets were identified. Examination of gene expression in each renal cell cancer subset and the pool of renal cell carcinoma samples compared with that in 17 different normal tissues revealed genes specifically overexpressed in renal cell cancer compared with these normal tissues. The authors conclude that gene expression patterns may be useful in helping to further classify subtypes of renal cell carcinoma that may have clinical significance. In addition, the genes identified as overexpressed in each set of clear cell renal cell carcinomas compared with normal tissues may represent useful targets for therapy.</p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 5","pages":"Pages 250-267"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2006.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26023344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphisms of p53 and p21 genes in chronic obstructive pulmonary disease","authors":"Yao-Ling Lee ,&nbsp;Wei Chen ,&nbsp;Wen-Kai Tsai ,&nbsp;Jen-Chih Lee ,&nbsp;Hui-Ling Chiou ,&nbsp;Chuen-Ming Shih ,&nbsp;Yi-Ching Wang","doi":"10.1016/j.lab.2005.12.008","DOIUrl":"10.1016/j.lab.2005.12.008","url":null,"abstract":"<div><p>Background: Chronic obstructive pulmonary disease (COPD) is a multifactorial disease influenced by genetic and environmental factors, particularly cigarette smoking. Although cigarette smoke may be directly mutagenic, polymorphisms in the genes controlling acquired somatic mutations may also contribute, at least to some extent, to the observed differing susceptibilities to COPD. To investigate the involvement of genetic polymorphisms of <em>p53</em> and <em>p21</em> in the pathogenesis of COPD, the authors performed a case-control study involving 206 subjects with COPD and 210 healthy smokers as control subjects. Methods: Polymorphisms of <em>p53</em> and <em>p21</em> genes were analyzed using the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) technique on genomic DNA isolated from peripheral lymphocytes. The distribution of the <em>p53</em> and <em>p21</em> polymorphisms in healthy subjects and COPD patients was examined and compared using the Pearson <em>X</em>² test. Significance was accepted at <em>P</em> &lt; 0.05. Odds ratios (ORs) and 95% confidence intervals (CIs) of each specific genotype were calculated using logistic regression analysis to quantitatively assess the degree of association observed. Results: The distribution frequencies of genotypes of <em>p53</em> codon 72 and <em>p21</em> codon 31 were significantly different between the COPD and the control groups. Higher ORs for COPD were seen for persons with p53 Pro/Pro or Pro/Arg genotypes against Arg/Arg genotype [OR = 2.35, 95% CI 1.27–4.39, <em>P</em> = 0.008], or p21 Arg/Arg and Arg/Ser genotypes against Ser/Ser genotype [OR = 2.07, 95% CI 1.06–4.05, <em>P</em> = 0.033]. Conclusions: The polymorphisms of <em>p53</em> and <em>p21</em> were significantly associated with the occurrence of smoking-related COPD in Taiwan Chinese patients.</p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 5","pages":"Pages 228-233"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2005.12.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26023339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amadori-modified glycated serum proteins and accelerated atherosclerosis in diabetes: Pathogenic and therapeutic implications","authors":"Margo P. Cohen ,&nbsp;Fuad N. Ziyadeh ,&nbsp;Sheldon Chen","doi":"10.1016/j.lab.2005.12.006","DOIUrl":"10.1016/j.lab.2005.12.006","url":null,"abstract":"","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 5","pages":"Pages 211-219"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2005.12.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26023341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"About the cover illustration","authors":"Dale E. Hammerschmidt MD (Editor-in-Chief)","doi":"10.1016/j.lab.2006.04.006","DOIUrl":"https://doi.org/10.1016/j.lab.2006.04.006","url":null,"abstract":"","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 5","pages":"Pages 268-269"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2006.04.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aspirin therapy for inhibition of platelet reactivity in the presence of erythrocytes in patients with vascular disease","authors":"M. Teresa Santos ,&nbsp;Juana Vallés ,&nbsp;Justo Aznar ,&nbsp;Aida Lago ,&nbsp;Elena Sanchez ,&nbsp;Juan Cosin ,&nbsp;Antonio Moscardó ,&nbsp;Marta Piñón ,&nbsp;M. Johan Broekman ,&nbsp;Aaron J. Marcus","doi":"10.1016/j.lab.2005.12.005","DOIUrl":"10.1016/j.lab.2005.12.005","url":null,"abstract":"<div><p>Inhibition of erythrocyte (RBC) promotion of platelet reactivity could improve the antiplatelet effect of aspirin (ASA). We tested different ASA regimens for optimal inhibition of platelets and the effects of RBC in patients with a history of vascular diseases. Collagen-induced platelet activation (¹⁴C-5HT, TXA₂ release) and platelet recruitment (proaggregatory activity of cell-free releasates from activated platelets) were measured in PRP, platelet-RBC (Hct 40%), and whole blood (WB) in 206 patients initially on 200–300-mg ASA/day. Their regimen was modified to biweekly 500 mg (loading dose, L) plus daily or twice-daily low-dose ASA (50 or 100 mg). TXA₂ was inhibited with all regimens. Percentage of patients with suboptimal inhibition of platelet recruitment in WB was 200–300 ASA/day (41%), L-50/day (87%), L-100/day (58%), L-50/twice-daily (39%), and L-100/twice-daily (20%; <em>P</em> &lt; 0.05 vs other regimens). ¹⁴C-5HT release was inhibited to the greatest extent with L-100/twice-daily in PRP + RBC or WB (<em>P</em> &lt; 0.05 vs other regimens) due to greater inhibition of the RBC prothrombotic effect. Compared with other ASA regimens, L-100 twice-daily (equivalent to 221-mg ASA/day in the 14-day cycle), reduced by &gt;50% the proportion of patients with suboptimal inhibition of platelet recruitment in WB and inhibited ¹⁴C-5HT release to the greatest extent.</p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 5","pages":"Pages 220-227"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2005.12.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26023340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of peripheral natural killer cells in primary Sjögren’s syndrome: Impaired NK cell activity and low NK cell number","authors":"Yasumori Izumi ,&nbsp;Hiroaki Ida ,&nbsp;Mingguo Huang ,&nbsp;Nozomi Iwanaga ,&nbsp;Fumiko Tanaka ,&nbsp;Kouichiro Aratake ,&nbsp;Kazuhiko Arima ,&nbsp;Mami Tamai ,&nbsp;Makoto Kamachi ,&nbsp;Hideki Nakamura ,&nbsp;Tomoki Origuchi ,&nbsp;Atsushi Kawakami ,&nbsp;Paul Anderson ,&nbsp;Katsumi Eguchi","doi":"10.1016/j.lab.2006.01.001","DOIUrl":"10.1016/j.lab.2006.01.001","url":null,"abstract":"<div><p>The aim of this study was to compare the number of peripheral blood natural killer (NK) cells, NK cell activity, expression of NK cell activating receptors, and serum cytokine levels in patients with primary Sjögren’s syndrome (SS) vs normal controls. The authors found that NK cell number, NK cell killing activity, and the expression of activating receptors CD2 and NKG2D were significantly decreased, and the expression of NKp46, as well as the percentage of apoptotic NK cells, were significantly increased in primary SS patients compared with healthy controls. NK cell killing activity on a per-cell basis was similar in primary SS patients and healthy controls. Moreover, the levels of IL-18 and TNF-α, cytokines that have been shown to promote NK cell death, were significantly increased in sera from patients with primary SS compared with controls. These data suggest that reduced NK cell numbers, probably a result of apoptotic death, may contribute to impaired NK cell activity in patients with primary SS.</p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 5","pages":"Pages 242-249"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2006.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26023343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Information for readers","authors":"","doi":"10.1016/S0022-2143(06)00169-7","DOIUrl":"https://doi.org/10.1016/S0022-2143(06)00169-7","url":null,"abstract":"","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 5","pages":"Page CO3"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0022-2143(06)00169-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term ethanol consumption and macrocytosis: diagnostic and pathogenic implications","authors":"Heidi Koivisto,&nbsp;Johanna Hietala,&nbsp;Petra Anttila,&nbsp;Seppo Parkkila,&nbsp;Onni Niemelä","doi":"10.1016/j.lab.2005.12.004","DOIUrl":"10.1016/j.lab.2005.12.004","url":null,"abstract":"<div><p>Although excessive alcohol consumption is known to elevate the mean cell volume (MCV) of erythrocytes, the relationships among the intensity of ethanol exposure, the generation of abnormal red blood cell indices, and the underlying pathogenic mechanisms have remained unclear. The authors examined 105 alcoholics with a wide range of ethanol consumption (40–500 g of ethanol/day), 62 moderate drinkers (mean consumption 1–40 g/day), and 24 abstainers, who underwent detailed interviews, measurements of blood cell counts, markers of liver status, and circulating antibodies against ethanol-derived protein modifications. Follow-up information was collected from healthy volunteers with detailed records on drinking habits. Data from the NORIP project for laboratory parameters in apparently healthy moderate drinkers or abstainers (n = 845) were used for reference interval comparisons. The highest MCV (<em>P</em> &lt; 0.001) and mean cell hemoglobin (MCH) (<em>P</em> &lt; 0.01) occurred in the alcoholics. However, the values in the moderate drinkers also responded to ethanol intake such that the upper normal limit for MCV based on the data from moderate drinkers was 98 fl, as compared with 96 fl from abstainers. Follow-up cases with carefully registered drinking habits showed parallel changes in MCV and ethanol intake. Anti-adduct IgA and IgM against acetaldehyde-induced protein modifications were elevated in 94% and 64% of patients with high MCV, respectively, the former being significantly less frequent in the alcoholics with normal MCV (63%) (<em>P</em> &lt; 0.05). The data indicate dose-related responses in red blood indices upon chronic ethanol consumption, which may also be reflected in reference intervals for hematological parameters in health care. Generation of immune responses against acetaldehyde-modified erythrocyte proteins may be associated with the appearance of such abnormalities.</p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 4","pages":"Pages 191-196"},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2005.12.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25944348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}