Journal of Laboratory Physicians最新文献

{"title":"Molecular Characterization of Extended Spectrum β-Lactamase Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolates and Their Antibiotic Resistance Profile in Health Care-Associated Urinary Tract Infections in North India.","authors":"Sheetal Verma,&nbsp;Raj Kumar Kalyan,&nbsp;Prashant Gupta,&nbsp;Mohd Danish Khan,&nbsp;Vimala Venkatesh","doi":"10.1055/s-0042-1757416","DOIUrl":"https://doi.org/10.1055/s-0042-1757416","url":null,"abstract":"<p><p><b>Background</b>  Healthcare-associated urinary tract infections (HAUTIs) caused by gram-negative pathogens have emerged as a global concern. So far, little is known about the epidemiology of extended-spectrum β lactamase (ESBL)-producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> in HAUTIs in India. The study was carried to determine the antibiotic resistance pattern and ESBL-producing genes in <i>E. coli</i> and <i>K. pneumoniae</i> strains isolated from HAUTIs in a tertiary institute in North India. <b>Methods</b>  A total of 200 consecutive, nonduplicate clinical isolates of <i>E. coli</i> and 140 isolates of <i>K. pneumoniae</i> from hospitalized patients with UTI were collected during a period of 1 year. Strains were studied for the presence of ESBL genes (blaCTX-M1, blaCTX-M2, blaCTX-M9, blaCTX-M15, blaSHV, blaTEM, blaOXA-1, blaVEB, blaPER-2, and blaGES) by multiplex polymerase chain reaction using gene-specific primers. <b>Results</b>  ESBL was detected in 82.5% (165 out of 200) isolates of <i>E. coli</i> and 74.3% (104 out of 140) isolates of <i>K. pneumoniae</i> by phenotypic confirmatory testing. From 269 phenotypically positive ESBL isolates, blaTEM (49.4%) was the most common genotype followed by blaCTX-M1 (31.97%), blaOXA-1 (30.1%), and blaSHV(11.9%) either alone or in combination. In the present study, blaCTX-M-15 (84.89%) was the most common blaCTX-M1-type ESBL. In total, 2.6 and 5.2% of the isolates were positive for <i>PER-2</i> and <i>VEB</i> genes, respectively. <b>Conclusion</b>  To the best of our knowledge, this is the first study on ESBL resistance patterns and ESBL-producing genes in HAUTIs in North India. Our study reports high occurrence with ESBL types CTX-M-1, CTX-M-15, TEM, and SHV. Minor ESBL variants OXA-1, VEB-type, and PER-2-type β-lactamase are also emerging in HAUTIs infections in North India.</p>","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/18/ac/10-1055-s-0042-1757416.PMC10264127.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9647089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematogones: The Supreme Mimicker and a Cytomorphological Confounder in Acute Lymphoblastic Leukemia.","authors":"Kanwaljeet Singh,&nbsp;Dwarika Tiwari,&nbsp;Revanth Boddu,&nbsp;Venkatesan Somasundarum,&nbsp;Kundan Mishra","doi":"10.1055/s-0042-1757586","DOIUrl":"https://doi.org/10.1055/s-0042-1757586","url":null,"abstract":"<p><p><b>Objective</b>  B-lymphocyte progenitors, namely the hematogones (HGs), may pose problems in morphological assessment of bone marrow, not only during the diagnostic workup but also while evaluating bone marrow for remission status following chemotherapy. Here, we describe a series of 12 cases of acute lymphoblastic leukemia (ALL) that included both B-ALL and T-ALL cases, which were evaluated for remission status and revealed blast-like mononuclear cells in bone marrow in the range of 6 to 26%, which on immunophenotypic analysis turned out to be HGs. <b>Materials and Methods</b>  This is a case series of 12 ALL cases who were undergoing treatment at the Army Hospital (Referral and Research), New Delhi. All these cases were under workup for post-induction status (day 28) and to check for suspected ALL relapse. Bone marrow aspirate (BMA), biopsy, and immunophenotyping were performed. Multicolored flow cytometry was performed using CD10, CD20, CD22, CD34, CD19, and CD38 antibodies panel. <b>Results</b>  BMA assessment of 12 cases revealed a maximum of 26% blastoid cells and a minimum of up to 6%, raising the suspicion of hematological relapse. However, on clinical assessment, these patients were well preserved, with preserved peripheral counts. Hence, marrow aspirates were subjected to flow cytometry using the CD markers panel, as discussed above, which revealed HGs. These cases were followed by minimal residual disease (MRD) analysis that revealed MRD-negative status, further confirming our findings. <b>Conclusion</b>  This case series highlights the importance of morphology and bone marrow immunophenotyping in unveiling the diagnostic dilemma in post-induction ALL patients.</p>","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3a/6a/10-1055-s-0042-1757586.PMC10264119.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9647094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategies to Decrease the Prevalence of Soil-Transmitted Helminths in Central India.","authors":"Archa Sharma,&nbsp;Shashank Purwar,&nbsp;Shipra Gupta,&nbsp;Ayush Gupta,&nbsp;Disha Gautam","doi":"10.1055/s-0042-1757417","DOIUrl":"https://doi.org/10.1055/s-0042-1757417","url":null,"abstract":"<p><p><b>Background</b>  Intestinal parasites are a major public health problem in tropical countries. Over 1.5 billion people are infected with soil-transmitted helminths (STH), of which 225 million are in India. Parasitic infections are associated with poor sanitation, lack of safe potable water, and improper hygiene. <b>Materials and Methods</b>  The study was undertaken to ascertain the impact of control strategies, namely open-defecation free drive and mass drug administration of single dose albendazole. Stool samples received at AIIMS Bhopal Microbiology laboratory, across all age groups, were studied for protozoan trophozoites/cysts and helminthic ova. <b>Results</b>  Out of 4,620 stool samples, 389 (8.41%) were positive either for protozoal or helminthic infections. Protozoan infections were more common than helminthic infections with <i>Giardia duodenalis</i> infection being the most common, 201 (51.67%), followed by <i>Entamoeba histolytica</i> , 174 (44.73%). The helminthic infections constituted 14 (3.5%) of the positive stool samples with Hookworm ova in 6 (1.5%) cases. <b>Conclusion</b>  This study proves that strategies, namely \"Swachh Bharat Abhiyan\" and \"National Deworming Day\" started in 2014 and 2015 led to significant reduction of intestinal parasite infections in Central India, with a higher reduction of STH compared with protozoan parasite infection being ascribed to the activity spectrum of albendazole.</p>","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2b/1c/10-1055-s-0042-1757417.PMC10264114.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9647087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Point Prevalence Study (PPS) of Antibiotic Usage and Bacterial Culture Rate (BCR) among Secondary Care Hospitals of Small Cities in Central India: Consolidating Indian Evidence.","authors":"Shweta Kumar,&nbsp;Pankaj Shukla,&nbsp;Pramod Goel,&nbsp;Vivek Mishra,&nbsp;Ayush Gupta,&nbsp;Tadepalli Karuna,&nbsp;Rakesh Srivastava,&nbsp;Amit Gupta,&nbsp;Deepak Baharani,&nbsp;Parijat Pansey,&nbsp;Sunil Chandiwal,&nbsp;Sandeep Shrivastava,&nbsp;Ankur Gupta,&nbsp;Shailendra Singh Rajpoot,&nbsp;DebaDulal Biswal,&nbsp;Mehrunnisa Ansari,&nbsp;Kamini Walia,&nbsp;Sagar Khadanga","doi":"10.1055/s-0042-1757585","DOIUrl":"https://doi.org/10.1055/s-0042-1757585","url":null,"abstract":"<p><p><b>Objective</b>  Indian hospitals (especially government-run public sector hospitals) have a nonexistent antimicrobial stewardship program (AMSP). After successfully initiating AMSPs in tertiary care hospitals of India, the Indian Council of Medical Research envisages implementing AMSP in secondary care hospitals. This study is about the baseline data on antibiotic consumption in secondary care hospitals. <b>Materials and Methods</b>  It was a prospective longitudinal observational chart review type of study. Baseline data on antibiotic consumption was captured by a 24-hour point prevalence study of antibiotic usage and bacterial culture rate. The prescribed antibiotics were classified according to the World Health Organization (WHO) Access, Watch, and Reserve classification. All data were collated in Microsoft Excel and summarized as percentages. <b>Results</b>  Out of the 864 patients surveyed, overall antibiotic usage was 78.9% (71.5% in low-priority areas vs. 92.2% in high-priority areas). Most of the antibiotic usage was empirical with an extremely low bacterial culture rate (21.9%). Out of the prescribed drugs, 53.1% were from the WHO watch category and 5.5% from the reserve category. <b>Conclusion</b>  Even after 5 years of the launch of the national action plan on AMR (NAP-AMR) of India, AMSP is still non-existent in small- and medium-level hospitals in urban cities. The importance of trained microbiologists in the health care system is identified as a fulcrum in combating antimicrobial resistance (AMR); however, their absence in government-run district hospitals is a matter of grave concern and needs to be addressed sooner than later.</p>","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/50/08/10-1055-s-0042-1757585.PMC10264115.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9653279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early and Effective Diagnosis of Sepsis Using Flow Cytometry.","authors":"Pratima Verma,&nbsp;Anurag Singh,&nbsp;Rashmi Kushwaha,&nbsp;Geeta Yadav,&nbsp;Shailendra P Verma,&nbsp;Uma S Singh,&nbsp;Himanshu D Reddy,&nbsp;Avinash Agarwal","doi":"10.1055/s-0042-1757722","DOIUrl":"https://doi.org/10.1055/s-0042-1757722","url":null,"abstract":"<p><p><b>Objective</b>  Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it is vital to diagnose and treat it as soon as possible. Blood cultures can take up to 2 days to give result, and they are not always reliable. According to recent studies, neutrophil CD64 expression might be a sensitive and specific option for assessing sepsis. This study aimed to evaluate the diagnostic performance of a flow cytometry analysis for the expression of neutrophil CD64 in sepsis and its comparison with other standard tests in a tertiary care center. <b>Materials and Methods</b>  Prospective analysis on 40 blood samples from suspected sepsis patients admitted to intensive care units with criteria for the systemic inflammatory response syndrome on presentation was performed for expression of neutrophil CD64, C-reactive protein, procalcitonin, and complete blood count. Ten healthy volunteers were also enrolled in this prospective study. The laboratory results were compared in different groups. <b>Results</b>  The neutrophil CD64 had the highest diagnostic value to differentiate between patients of sepsis and nonsepsis groups with a sensitivity of 100% (95% confidence interval [CI]: 77.19-100%) and 100% (95% CI: 55.32-86.83%); specificity of 90.00% (95% CI: 59.58-99.49%) and 87.24% (95% CI: 66.69-99.61%); and likelihood ratio of 10.00 and 7.84, respectively. <b>Conclusion</b>  The neutrophil CD64 expression provides a more sensitive, specific, and novel marker for the early detection of sepsis in critically ill patients.</p>","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/51/40/10-1055-s-0042-1757722.PMC10264123.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9653280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multilocular Cystic Renal Neoplasm of Low Malignant Potential in a Patient with Chronic Pyelonephritis and End-Stage Renal Disease: A Rare Case Report","authors":"R. Kaur, V. Nijhawan, Ayushi Kediya, Vishesh Dhawan, Sahil Singhal, Ashmita Joshi","doi":"10.1055/s-0043-1772850","DOIUrl":"https://doi.org/10.1055/s-0043-1772850","url":null,"abstract":"Abstract Multilocular cystic renal neoplasm of low malignant potential (MCNLMP) is a rare subtype of clear cell carcinoma having an excellent prognosis. These tumors exhibit distinct morphology and comprises of multiple cysts separated by thin septa lined by clear cells with no expansile growth. These cysts, though rare in a setting of chronic pyelonephritis and end-stage renal disease, are usually diagnosed on radiography using the Bosniak classification. But in certain situations where cysts are not visible radiologically, a final diagnosis is made with the help of histopathology and immunohistochemistry only. We, hereby, report such a rare incidental case of MCNLMP diagnosed on histopathology in a nephrectomy specimen of an elderly male with chronic pyelonephritis and end-stage renal disease.","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45181136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenomatoid Tumor of Fallopian Tube with Intratumoral Endometriosis: What Mind Doesn't Know, Eyes Don't See","authors":"M. Osama, Seema Rao, Mamta Dagar","doi":"10.1055/s-0043-1772217","DOIUrl":"https://doi.org/10.1055/s-0043-1772217","url":null,"abstract":"Abstract Adenomatoid tumor, a benign tumor of mesothelial origin, is seen most commonly in paratesticular tissue in males and uterus in females. Its incidence is extremely rare in the fallopian tube, with only few such case reports. In the present case, an incidental association of adenomatoid tumor of the fallopian tube with turbo-ovarian endometriosis and ipsilateral nonfunctioning kidney was seen. Intraoperatively, a small nodular lesion was seen over the tubal wall. On a detailed review of literature, we found very few cases of adenomatoid tumor of the fallopian tube. The other unique finding was the occurrence of intratumoral endometriosis within the adenomatoid tumor.","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41325813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Polyethylene Glycol and Enzymes in Identifying Antibody Specificity in a Patient with Sickle Cell Disease","authors":"Sree Bhagavathi, S. Mukherjee, S. Prakash, A. Sahu, Debasish Mishra, Priyanka Nagrath","doi":"10.1055/s-0043-1772563","DOIUrl":"https://doi.org/10.1055/s-0043-1772563","url":null,"abstract":"Abstract Alloimmunization is a common problem in sickle cell disease (SCD) patients. Providing antigen-negative units in this group of patients makes it challenging for the transfusion laboratory. Antibody identification is required to decrease the incidence of hemolytic transfusion reaction in case of alloimmunization. In SCD patients, identification of antibody specificity is essential to prevent particularly delayed type transfusion reactions. This is a case report of a transfusion-dependent SCD patient whose antibody specificity could not be determined on initial antibody identification testing. A modified antibody identification test was done with polyethylene glycol (PEG) and enzyme to identify antibody specificity in this patient. Alloantibody anti-c was identified and reported to the clinician. The patient was transfused with c antigen-negative blood. This case report highlights the role of PEG and enzyme treatment in antibody identification for transfusion support in this group of highly alloimmunized patients.","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49664458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive Value of Adiposity Index in Identifying Depression in Individuals with Type 2 Diabetes Mellitus in Indian Population","authors":"Jiya Singh, P. Singh, Rashmi Nain, Ravi Kant, Anindya Das, A. Mirza, S. Saha","doi":"10.1055/s-0043-1771402","DOIUrl":"https://doi.org/10.1055/s-0043-1771402","url":null,"abstract":"Abstract Objectives  Type 2 diabetes mellitus (T2DM) patients are four times more prone to develop depression. Common subjective tool to evaluate depression is Patient Health Questionnaire-9 (PHQ-9). Depression in T2DM remains unaddressed because of lack of objective tools resulting in poor treatment compliance. Both obesity and metabolic disturbances could influence mental health status. Therefore, this study was designed to determine the better adiposity index to predict depression in T2DM. Subjects and Methods  In this clinic-based cross-sectional study, 400 individuals (260 = T2DM and 140 = healthy) were recruited. Based on PHQ-9, T2DM patients were divided into T2DM + Dep (PHQ-9 ≥ 10) and T2DM-Dep (PHQ-9 < 10). The relationship between the PHQ-9 score and adiposity indices was examined by Pearson's/ Spearman's correlation. The receiver-operating characteristic curve analysis was used to identify the cutoff value. Results  Female diabetic patients showed significant correlation only in lipid accumulation product index (LAPI) and visceral adiposity index (VAI) ( r  = 0.206 and r  = 0.0.237, respectively), while male diabetic patients did not show any significant association. Interestingly, T2DM + Dep group showed significant association between LAPI ( r  = 0.248) with PHQ-9 score, while T2DM-Dep group did not show significant association. VAI had maximum area under the curve in T2DM patients (0.619, p  = 0.002) as well as in female diabetic patients (0.684, p  = 0.002). The cutoff value for identifying depression among diabetic individuals was 5.60, with 70.3% sensitivity and 48.2% specificity, while in diabetic females, it was 6.612, with 70% sensitivity and 61.4% specificity. Conclusion  VAI might be the best adiposity index to predict depression among diabetic individuals.","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43060727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unrevealing the Role of miRNA in Successful TNBC Treatment: A Pilot Study to Explore the Chemotherapy Drugs for Timely Treatment of TNBC","authors":"Poulami Sarkar, Rashmi Chowdhary, Ashish Kumar Yadav, Neha Arya, Bharti Pandya, Vinay Kumar, J. Kanwar, Arshi Siddiqui, Roji Begum Khan","doi":"10.1055/s-0043-1774405","DOIUrl":"https://doi.org/10.1055/s-0043-1774405","url":null,"abstract":"Abstract Objective  Worldwide, breast cancer is the most prevalent and common type of cancer. Physical examination and mammography with a range of sensitivities are currently used as screening methods. Triple-negative breast cancer (TNBC) lacks estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER-2) gene expression. MicroRNAs (miRNA) as potential prognostic and diagnostic biomarkers, miRNA 125, 200c, 221, 21, and 34a were selected for study. Materials and Methods  Here, 25 consenting TNBC patients with negative ER/PR/HER-2 status and compatible history were accrued from the Department of Oncosurgery, All India Institutes of Medical Sciences (AIIMS) Bhopal. Serum from participants and 25 controls was collected for quantitative estimation of miRNA by quantitative real-time polymerase chain reaction. After being treated with epirubicin, capecitabine, and paclitaxel, the MDA-MB-231 cell line's expression of these miRNA subtypes was also examined. Results  miRNA125 ( p < 0.0001) and miRNA21 ( p < 0.05) were found to be statistically significant. miR125 (ΔCt [cycle threshold] 2.77) was seen to be upregulated and miR21 (ΔCt -1.61) was seen to be downregulated in TNBC patients. Epirubicin treatment caused miR125 to be downregulated, but capecitabine treatment caused miR125 to be upregulated. Paclitaxel was seen to downregulate miR21. All three chemotherapeutic agents were seen to downregulate miR34a. Conclusion  miRNAs can be developed into a reliable biomarker and prognostic tool with more research. They can also help develop and improve pharmaco-therapeutic strategies.","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"57982963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}