Wei-Min Fu, Shengnan Huang, Zhiyong Liu, Yue Gao, Meidi Zhang, Nan Wang, Gaoyang Qu, H. Feng
{"title":"Fine mapping of Brebm6, a gene conferring the early-bolting phenotype in Chinese cabbage (Brassica rapa ssp. pekinensis)","authors":"Wei-Min Fu, Shengnan Huang, Zhiyong Liu, Yue Gao, Meidi Zhang, Nan Wang, Gaoyang Qu, H. Feng","doi":"10.48130/vr-2021-0007","DOIUrl":"https://doi.org/10.48130/vr-2021-0007","url":null,"abstract":"In flowering plants, bolting signals the transition from vegetative to reproductive growth, and it is consequently an important agronomic trait for the stalk-use Chinese cabbage Baicaitai. There is an urgent need to create germplasm resources for Chinese cabbage that have easy bolting characteristics. In this investigation, early bolting mutant 6 (ebm6) was created by treating Chinese cabbage double haploid (DH) 'FT' seeds with an ethyl methanesulfonate (EMS) solution. Phenotypic analysis showed that the mutant ebm6 flowered remarkably earlier than the wild-type 'FT'. Genetic analysis indicated that this early bolting trait was controlled by a recessive nuclear gene. In mutant ebm6, the causal gene BraA02g003340.3C (BrFLC2) was predicted using MutMap and Kompetitive Allele Specific PCR genotyping (KASP), as the gene encodes a MADS-box transcription factor. The 3259-bp BrFLC2 gene possessed seven exons and six introns, and in the first exon, a single nucleotide C-to-T substitution in the highly conserved MADS-box domain resulted in the premature termination of translation of the BrFLC2 protein sequence. This research supported a critical role of BrFLC2 in controlling flowering time in B. rapa. The early flowering ebm6 mutant could also be a useful material in Baicaitai breeding.","PeriodicalId":160756,"journal":{"name":"Vegetable Research","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128788303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An effective method for studying gene function of Chinese cabbage","authors":"Tongkun Liu","doi":"10.48130/vr-2023-0002","DOIUrl":"https://doi.org/10.48130/vr-2023-0002","url":null,"abstract":"","PeriodicalId":160756,"journal":{"name":"Vegetable Research","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133096620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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