Journal of immunology最新文献

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Enhancement of Autophagy in Macrophages via the p120-Catenin-Mediated mTOR Signaling Pathway. 通过 p120-Catenin 介导的 mTOR 信号通路增强巨噬细胞的自噬作用
IF 3.6 3区 医学
Journal of immunology Pub Date : 2024-12-01 DOI: 10.4049/jimmunol.2400189
Suganya Kanmani, Xue-Min Song, Paulraj Kanmani, Xiao-Jing Wu, Xiao-Di Tan, Jing Liu, Ji-Ping Wang, Richard D Minshall, Guochang Hu
{"title":"Enhancement of Autophagy in Macrophages via the p120-Catenin-Mediated mTOR Signaling Pathway.","authors":"Suganya Kanmani, Xue-Min Song, Paulraj Kanmani, Xiao-Jing Wu, Xiao-Di Tan, Jing Liu, Ji-Ping Wang, Richard D Minshall, Guochang Hu","doi":"10.4049/jimmunol.2400189","DOIUrl":"10.4049/jimmunol.2400189","url":null,"abstract":"<p><p>Autophagy serves as a critical regulator of immune responses in sepsis. Macrophages are vital constituents of both innate and adaptive immunity. In this study, we delved into the intricate role of p120-catenin (p120) in orchestrating autophagy in macrophages in response to endotoxin stimulation. Depletion of p120 effectively suppressed LPS-induced autophagy in both J774A.1 macrophages and murine bone marrow-derived macrophages. LPS not only elevated the interaction between p120 and L chain 3 (LC3) I/II but also facilitated the association of p120 with mammalian target of rapamycin (mTOR). p120 depletion in macrophages by small interfering RNA reduced LPS-induced dissociation of mTOR and Unc-51-like kinase 1 (ULK1), leading to an increase in the phosphorylation of ULK1. p120 depletion also enhanced LPS-triggered macrophage apoptosis, as evidenced by increased levels of cleaved caspase 3, 7-aminoactinomycin D staining, and TUNEL assay. Notably, inhibiting autophagy reversed the decrease in apoptosis caused by LPS stimulation in macrophages overexpressing p120. Additionally, the ablation of p120 inhibited autophagy and accentuated apoptosis in alveolar macrophages in LPS-challenged mice. Collectively, our findings strongly suggest that p120 plays a pivotal role in fostering autophagy while concurrently hindering apoptosis in macrophages, achieved through modulation of the mTOR/ULK1 signaling pathway in sepsis. This underscores the potential of targeting macrophage p120 as an innovative therapeutic avenue for treating inflammatory disorders.</p>","PeriodicalId":16045,"journal":{"name":"Journal of immunology","volume":" ","pages":"1666-1675"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11610512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Virus-specific Th17 Cells Are Induced by Human Cytomegalovirus after Renal Transplantation. 肾移植后人类巨细胞病毒会诱发病毒特异性 Th17 细胞
IF 3.6 3区 医学
Journal of immunology Pub Date : 2024-12-01 DOI: 10.4049/jimmunol.2300742
Ravi Dhital, Kaitlyn Flint, Irina Kaptsan, Shweta Hegde, Reem Daloul, Masako Shimamura
{"title":"Virus-specific Th17 Cells Are Induced by Human Cytomegalovirus after Renal Transplantation.","authors":"Ravi Dhital, Kaitlyn Flint, Irina Kaptsan, Shweta Hegde, Reem Daloul, Masako Shimamura","doi":"10.4049/jimmunol.2300742","DOIUrl":"10.4049/jimmunol.2300742","url":null,"abstract":"<p><p>CMV infection and Th17 cells are independently associated with increased risk for late allograft loss after renal transplantation. Although CMV-specific Th17 cells are detectable in animal models and nontransplant clinical populations, evidence linking CMV and Th17 cells after renal transplantation remains unclear. This prospective observational study evaluated a cohort of renal transplant recipients during 12 mo posttransplant to assess the presence of CMV-specific Th17 cells in peripheral blood and their relationship to pretransplant CMV serostatus and CMV DNAemia. CMV-specific Th17 cells were identified among CMV serostatus donor (D)+ and/or recipient (R)+ recipients and expanded during both primary (D+/R-) and reactivated (D+/R+, D-/R+) CMV DNAemia. A subset of CMV-specific Th17 cells coexpressed IFN-γ, indicating a Th1/17 phenotype. These Th17 and Th1/17 cells expressed CCR6, CCR5, activation and terminal differentiation markers (CD95, OX40, HLA-DR, CD57), and a central/effector memory phenotype. CMV-specific Th1/17 cells expressed activating/inhibitory receptors (CD57, 4-1BB, CD160, CTLA-4, PD-1) at higher frequencies than Th17 cells. In contrast, staphylococcal enterotoxin B-induced Th17 cells did not expand during CMV DNAemia, did not differ between CMV serostatus groups over time, expressed CCR6, predominantly coexpressed TNF-α, and had lower expression of activating and inhibitory receptors than pp65-specific Th17 and Th1/17 cells. These data show that CMV-specific Th17 cells expand during episodes of CMV DNAemia among renal transplant recipients, and that these virus-specific Th17 and Th1/17 cells have distinct phenotypes from global circulating Th(1)/17 cells. These results suggest a potential proinflammatory pathway by which CMV-induced Th17 cells may contribute to allograft injury, increasing risk for late allograft loss.</p>","PeriodicalId":16045,"journal":{"name":"Journal of immunology","volume":" ","pages":"1703-1712"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11573647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-cell Sequencing of Circulating Human Plasmablasts during Staphylococcus aureus Bacteremia. 金黄色葡萄球菌菌血症期间循环人体浆细胞的单细胞测序。
IF 3.6 3区 医学
Journal of immunology Pub Date : 2024-12-01 DOI: 10.4049/jimmunol.2300858
Priscilla F Kerkman, Lisanne de Vor, Thomas W van der Vaart, Thijs Ten Doesschate, Remy M Muts, Jamie S Depelteau, Lisette M Scheepmaker, Maartje Ruyken, Carla J C de Haas, Piet C Aerts, Renoud J Marijnissen, Janine Schuurman, Frank J Beurskens, Andrea Gorlani, Bart W Bardoel, Suzan H M Rooijakkers
{"title":"Single-cell Sequencing of Circulating Human Plasmablasts during Staphylococcus aureus Bacteremia.","authors":"Priscilla F Kerkman, Lisanne de Vor, Thomas W van der Vaart, Thijs Ten Doesschate, Remy M Muts, Jamie S Depelteau, Lisette M Scheepmaker, Maartje Ruyken, Carla J C de Haas, Piet C Aerts, Renoud J Marijnissen, Janine Schuurman, Frank J Beurskens, Andrea Gorlani, Bart W Bardoel, Suzan H M Rooijakkers","doi":"10.4049/jimmunol.2300858","DOIUrl":"10.4049/jimmunol.2300858","url":null,"abstract":"<p><p>Staphylococcus aureus is the major cause of healthcare-associated infections, including life-threatening conditions as bacteremia, endocarditis, and implant-associated infections. Despite adequate antibiotic treatment, the mortality of S. aureus bacteremia remains high. This calls for different strategies to treat this infection. In past years, sequencing of Ab repertoires from individuals previously exposed to a pathogen emerged as a successful method to discover novel therapeutic monoclonal Abs and understand circulating B cell diversity during infection. In this paper, we collected peripheral blood from 17 S. aureus bacteremia patients to study circulating plasmablast responses. Using single-cell transcriptome gene expression combined with sequencing of variable heavy and light Ig genes, we retrieved sequences from >400 plasmablasts revealing a high diversity with >300 unique variable heavy and light sequences. More than 200 variable sequences were synthesized to produce recombinant IgGs that were analyzed for binding to S. aureus whole bacterial cells. This revealed four novel monoclonal Abs that could specifically bind to the surface of S. aureus in the absence of Ig-binding surface SpA. Interestingly, three of four mAbs showed cross-reactivity with Staphylococcus epidermidis. Target identification revealed that the S. aureus-specific mAb BC153 targets wall teichoic acid, whereas cross-reactive mAbs BC019, BC020, and BC021 target lipoteichoic acid. All mAbs could induce Fc-dependent phagocytosis of staphylococci by human neutrophils. Altogether, we characterize the active B cell responses to S. aureus in infected patients and identify four functional mAbs against the S. aureus surface, of which three cross-react with S. epidermidis.</p>","PeriodicalId":16045,"journal":{"name":"Journal of immunology","volume":" ","pages":"1644-1655"},"PeriodicalIF":3.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7616744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
NK Cell and Monocyte Dysfunction in Multisystem Inflammatory Syndrome in Children. 儿童多系统炎症综合征中的 NK 细胞和单核细胞功能障碍
IF 3.6 3区 医学
Journal of immunology Pub Date : 2024-11-15 DOI: 10.4049/jimmunol.2400395
Jenna K Dick, Jules A Sangala, Venkatramana D Krishna, Aaron Khaimraj, Lydia Hamel, Spencer M Erickson, Dustin Hicks, Yvette Soigner, Laura E Covill, Alexander K Johnson, Michael J Ehrhardt, Keenan Ernste, Petter Brodin, Richard A Koup, Alka Khaitan, Carly Baehr, Beth K Thielen, Christine M Henzler, Caleb Skipper, Jeffrey S Miller, Yenan T Bryceson, Jianming Wu, Chandy C John, Angela Panoskaltsis-Mortari, Alberto Orioles, Marie E Steiner, Maxim C J Cheeran, Marco Pravetoni, Geoffrey T Hart
{"title":"NK Cell and Monocyte Dysfunction in Multisystem Inflammatory Syndrome in Children.","authors":"Jenna K Dick, Jules A Sangala, Venkatramana D Krishna, Aaron Khaimraj, Lydia Hamel, Spencer M Erickson, Dustin Hicks, Yvette Soigner, Laura E Covill, Alexander K Johnson, Michael J Ehrhardt, Keenan Ernste, Petter Brodin, Richard A Koup, Alka Khaitan, Carly Baehr, Beth K Thielen, Christine M Henzler, Caleb Skipper, Jeffrey S Miller, Yenan T Bryceson, Jianming Wu, Chandy C John, Angela Panoskaltsis-Mortari, Alberto Orioles, Marie E Steiner, Maxim C J Cheeran, Marco Pravetoni, Geoffrey T Hart","doi":"10.4049/jimmunol.2400395","DOIUrl":"10.4049/jimmunol.2400395","url":null,"abstract":"<p><p>Multisystem inflammatory syndrome in children (MIS-C) is a severe complication of SARS-CoV-2 infection characterized by multiorgan involvement and inflammation. Testing of cellular function ex vivo to understand the aberrant immune response in MIS-C is limited. Despite strong Ab production in MIS-C, SARS-CoV-2 nucleic acid testing can remain positive for 4-6 wk postinfection. Therefore, we hypothesized that dysfunctional cell-mediated Ab responses downstream of Ab production may be responsible for delayed clearance of viral products in MIS-C. In MIS-C, monocytes were hyperfunctional for phagocytosis and cytokine production, whereas NK cells were hypofunctional for both killing and cytokine production. The decreased NK cell cytotoxicity correlated with an NK exhaustion marker signature and systemic IL-6 levels. Potentially providing a therapeutic option, cellular engagers of CD16 and SARS-CoV-2 proteins were found to rescue NK cell function in vitro. Taken together, our results reveal dysregulation in Ab-mediated cellular responses of myeloid and NK cells that likely contribute to the immune pathology of this disease.</p>","PeriodicalId":16045,"journal":{"name":"Journal of immunology","volume":" ","pages":"1452-1466"},"PeriodicalIF":3.6,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vivo CRISPR Screening Reveals CHD7 as a Positive Regulator of Short-lived Effector Cells. 体内 CRISPR 筛选发现 CHD7 是短寿命效应细胞的正向调节器。
IF 3.6 3区 医学
Journal of immunology Pub Date : 2024-11-15 DOI: 10.4049/jimmunol.2400213
Martin W LaFleur, Jasmin M D'Andrea, Dillon G Patterson, Ivy S L Streeter, Matthew A Coxe, Jossef F Osborn, Lauren E Milling, Qin Tjokrosurjo, Jacob E Gillis, Thao H Nguyen, Marc A Schwartz, Nir Hacohen, John G Doench, Arlene H Sharpe
{"title":"In Vivo CRISPR Screening Reveals CHD7 as a Positive Regulator of Short-lived Effector Cells.","authors":"Martin W LaFleur, Jasmin M D'Andrea, Dillon G Patterson, Ivy S L Streeter, Matthew A Coxe, Jossef F Osborn, Lauren E Milling, Qin Tjokrosurjo, Jacob E Gillis, Thao H Nguyen, Marc A Schwartz, Nir Hacohen, John G Doench, Arlene H Sharpe","doi":"10.4049/jimmunol.2400213","DOIUrl":"10.4049/jimmunol.2400213","url":null,"abstract":"<p><p>CD8+ T cells differentiate into two subpopulations in response to acute viral infection: memory precursor effector cells (MPECs) and short-lived effector cells (SLECs). MPECs and SLECs are epigenetically distinct; however, the epigenetic regulators required for formation of these subpopulations are mostly unknown. In this study, we performed an in vivo CRISPR screen in murine naive CD8+ T cells to identify the epigenetic regulators required for MPEC and SLEC formation, using the acute lymphocytic choriomeningitis virus Armstrong infection model. We identified the ATP-dependent chromatin remodeler CHD7 (chromodomain-helicase DNA-binding protein 7) as a positive regulator of SLEC formation, as knockout (KO) of Chd7 reduced SLECs numerically. In contrast, KO of Chd7 increased the formation of central memory T cells following pathogen clearance yet attenuated memory cell expansion following a rechallenge. These findings establish CHD7 as a novel positive regulator of SLEC and a negative regulator of central memory T cell formation.</p>","PeriodicalId":16045,"journal":{"name":"Journal of immunology","volume":" ","pages":"1528-1541"},"PeriodicalIF":3.6,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IL-1β Induces Human Endothelial Surface Expression of IL-15 by Relieving let-7c-3p Suppression of Protein Translation. IL-1β 通过缓解 let-7c-3p 对蛋白质翻译的抑制,诱导人内皮细胞表面表达 IL-15。
IF 3.6 3区 医学
Journal of immunology Pub Date : 2024-11-01 DOI: 10.4049/jimmunol.2400331
Clancy W Mullan, Luanna Summer, Francesc Lopez-Giraldez, Zuzana Tobiasova, Thomas D Manes, Shruthi Yasothan, Guiyu Song, Daniel Jane-Wit, W Mark Saltzman, Jordan S Pober
{"title":"IL-1β Induces Human Endothelial Surface Expression of IL-15 by Relieving let-7c-3p Suppression of Protein Translation.","authors":"Clancy W Mullan, Luanna Summer, Francesc Lopez-Giraldez, Zuzana Tobiasova, Thomas D Manes, Shruthi Yasothan, Guiyu Song, Daniel Jane-Wit, W Mark Saltzman, Jordan S Pober","doi":"10.4049/jimmunol.2400331","DOIUrl":"10.4049/jimmunol.2400331","url":null,"abstract":"<p><p>Expression of IL-15 on the surface of human graft endothelial cells (ECs) bound to the IL-15Rα subunit can increase the activation of CTLs, potentiating allograft rejection. Our previous work showed that surface expression of this protein complex could be induced by alloantibody-mediated complement activation through increased IL-1β synthesis, secretion, and autocrine/paracrine IL-1-mediated activation of NF-κB. In this article, we report that cultured human ECs express eight differently spliced IL-15 transcripts. Remarkably, IL-1β does not alter the expression level of any IL-15 transcript but induces surface expression independently of RNA polymerase II-mediated transcription while requiring new protein translation. Mechanistically, IL-1β causes an NF-κB-mediated reduction in the level of microRNA Let-7c-3p, thereby relieving a block of translation of IL-15 surface protein. Let7c-3p anti-miR can induce EC surface expression of IL-15/IL-15Rα in the absence of complement activation or of IL-1, enabling IL-15 transpresentation to boost CD8 T cell activation. Because of the complexity we have uncovered in IL-15 regulation, we recommend caution in interpreting increased total IL-15 mRNA or protein levels as a surrogate for transpresentation.</p>","PeriodicalId":16045,"journal":{"name":"Journal of immunology","volume":" ","pages":"1338-1348"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11493510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The G Protein-Coupled Receptor GPR56 Is an Inhibitory Checkpoint for NK Cell Migration. G 蛋白偶联受体 GPR56 是 NK 细胞迁移的抑制性检查点
IF 3.6 3区 医学
Journal of immunology Pub Date : 2024-11-01 DOI: 10.4049/jimmunol.2400228
Daniel Palacios, Rakesh Kumar Majhi, Edina K Szabo, Dennis Clement, Mieszko Lachota, Herman Netskar, Leena Penna, Silje Z Krokeide, Marianna Vincenti, Lise Kveberg, Karl-Johan Malmberg
{"title":"The G Protein-Coupled Receptor GPR56 Is an Inhibitory Checkpoint for NK Cell Migration.","authors":"Daniel Palacios, Rakesh Kumar Majhi, Edina K Szabo, Dennis Clement, Mieszko Lachota, Herman Netskar, Leena Penna, Silje Z Krokeide, Marianna Vincenti, Lise Kveberg, Karl-Johan Malmberg","doi":"10.4049/jimmunol.2400228","DOIUrl":"10.4049/jimmunol.2400228","url":null,"abstract":"<p><p>G protein-coupled receptors (GPCRs) represent the largest family of surface receptors and are responsible for key physiological functions, including cell growth, neurotransmission, hormone release, and cell migration. The GPCR 56 (GPR56), encoded by ADGRG1, is an adhesion GPCR found on diverse cell types, including neural progenitor cells, melanoma cells, and lymphocytes, such as effector memory T cells, γδ T cells, and NK cells. Using RNA-sequencing and high-resolution flow cytometry, we found that GPR56 mRNA and protein expression increased with NK cell differentiation, reaching its peak in adaptive NK cells. Small interfering RNA silencing of GPR56 led to increased spontaneous and chemokine-induced migration, suggesting that GPR56 functions as an upstream checkpoint for migration of highly differentiated NK cells. Increased NK cell migration could also be induced by agonistic stimulation of GPR56 leading to rapid internalization and deactivation of the receptor. Mechanistically, GPR56 ligation and downregulation were associated with transcriptional coactivator with PDZ-binding motif translocation to the nucleus and increased actin polymerization. Together, these data provide insights into the role of GPR56 in the migratory behavior of human NK cell subsets and may open possibilities to improve NK cell infiltration into cancer tissues by releasing a migratory checkpoint.</p>","PeriodicalId":16045,"journal":{"name":"Journal of immunology","volume":" ","pages":"1349-1357"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11491499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chitinase 3-like-1 Inhibits Innate Antitumor and Tissue Remodeling Immune Responses by Regulating CD47-SIRPα- and CD24-Siglec10-Mediated Phagocytosis. 几丁质酶 3-like-1 通过调节 CD47-SIRPα 和 CD24-Siglec10 介导的吞噬作用抑制先天性抗肿瘤和组织重塑免疫反应
IF 3.6 3区 医学
Journal of immunology Pub Date : 2024-11-01 DOI: 10.4049/jimmunol.2400035
Bing Ma, Suchitra Kamle, Takayuki Sadanaga, Chang-Min Lee, Joyce H Lee, Daniel C Yee, Zhou Zhu, Edwin K Silverman, Dawn L DeMeo, Augustine M K Choi, Chun Geun Lee, Jack A Elias
{"title":"Chitinase 3-like-1 Inhibits Innate Antitumor and Tissue Remodeling Immune Responses by Regulating CD47-SIRPα- and CD24-Siglec10-Mediated Phagocytosis.","authors":"Bing Ma, Suchitra Kamle, Takayuki Sadanaga, Chang-Min Lee, Joyce H Lee, Daniel C Yee, Zhou Zhu, Edwin K Silverman, Dawn L DeMeo, Augustine M K Choi, Chun Geun Lee, Jack A Elias","doi":"10.4049/jimmunol.2400035","DOIUrl":"10.4049/jimmunol.2400035","url":null,"abstract":"<p><p>Innate immune responses such as phagocytosis are critically linked to the generation of adaptive immune responses against the neoantigens in cancer and the efferocytosis that is essential for homeostasis in diseases characterized by lung injury, inflammation, and remodeling as in chronic obstructive pulmonary disease (COPD). Chitinase 3-like-1 (CHI3L1) is induced in many cancers where it inhibits adaptive immune responses by stimulating immune checkpoint molecules (ICPs) and portends a poor prognosis. CHI3L1 is also induced in COPD where it regulates epithelial cell death. In this study, we demonstrate that pulmonary melanoma metastasis inhibits macrophage phagocytosis by stimulating the CD47-SIRPα and CD24-Siglec10 phagocytosis checkpoint pathways while inhibiting macrophage \"eat me\" signals from calreticulin and HMGB1. We also demonstrate that these effects on macrophage phagocytosis are associated with CHI3L1 stimulation of the SHP-1 and SHP-2 phosphatases and inhibition of the accumulation and phosphorylation of cytoskeleton-regulating nonmuscle myosin IIa. This inhibition of innate immune responses such as phagocytosis provides a mechanistic explanation for the ability of CHI3L1 to stimulate ICPs and inhibit adaptive immune responses in cancer and diseases such as COPD. The ability of CHI3L1 to simultaneously inhibit innate immune responses, stimulate ICPs, inhibit T cell costimulation, and regulate a number of other oncogenic and inflammation pathways suggests that CHI3L1-targeted therapeutics are promising interventions in cancer, COPD, and other disorders.</p>","PeriodicalId":16045,"journal":{"name":"Journal of immunology","volume":" ","pages":"1279-1291"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Type I IFN Induces TCR-dependent and -independent Antimicrobial Responses in γδ Intraepithelial Lymphocytes. I 型 IFN 在γδ上皮内淋巴细胞中诱导依赖和不依赖 TCR 的抗微生物反应。
IF 3.6 3区 医学
Journal of immunology Pub Date : 2024-11-01 DOI: 10.4049/jimmunol.2400138
Matthew A Fischer, Luo Jia, Karen L Edelblum
{"title":"Type I IFN Induces TCR-dependent and -independent Antimicrobial Responses in γδ Intraepithelial Lymphocytes.","authors":"Matthew A Fischer, Luo Jia, Karen L Edelblum","doi":"10.4049/jimmunol.2400138","DOIUrl":"10.4049/jimmunol.2400138","url":null,"abstract":"<p><p>Intraepithelial lymphocytes (IELs) expressing the TCRγδ survey the intestinal epithelium to limit the invasion of microbial pathogens. The production of type I IFN is a central component of an antiviral immune response, yet how these proinflammatory cytokines contribute to γδ IEL effector function remains unclear. Based on the unique activation status of IELs and their ability to bridge innate and adaptive immunity, we investigated the extent to which type I IFN signaling modulates γδ IEL function. Using an ex vivo culture model, we find that type I IFN alone is unable to drive IFN-γ production, yet low-level TCR activation synergizes with type I IFN to induce IFN-γ production in murine γδ IELs. Further investigation into the underlying molecular mechanisms of costimulation revealed that TCRγδ-mediated activation of NFAT and JNK is required for type I IFN to promote IFN-γ expression in a STAT4-dependent manner. Whereas type I IFN rapidly upregulates antiviral gene expression independent of a basal TCRγδ signal, neither tonic TCR triggering nor the presence of a TCR agonist was sufficient to elicit type I IFN-induced IFN-γ production in vivo. However, bypassing proximal TCR signaling events synergized with IFNAR/STAT4 activation to induce γδ IEL IFN-γ production. These findings indicate that γδ IELs contribute to host defense in response to type I IFN by mounting a rapid antimicrobial response independent of TCRγδ signaling, and may produce IFN-γ in a TCR-dependent manner under permissive conditions.</p>","PeriodicalId":16045,"journal":{"name":"Journal of immunology","volume":" ","pages":"1380-1391"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11493514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IL-17: A Critical Cytokine for Defense against Oral Candidiasis. IL-17:防御口腔念珠菌病的关键细胞因子
IF 3.6 3区 医学
Journal of immunology Pub Date : 2024-10-15 DOI: 10.4049/jimmunol.2400510
Lucas Dos Santos Dias, Michail S Lionakis
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