Journal of HerbMed Pharmacology最新文献_第3页

Anti-atherosclerotic effects of Camellia chrysantha and Gynostemma pentaphyllum extracts mixture 山茶和绞股蓝混合提取物的抗动脉粥样硬化作用

Journal of HerbMed Pharmacology Pub Date : 2023-08-10 DOI: 10.34172/jhp.2023.44843

Hong Hanh Nguyen, Ngan Hoang Nguyen, Tu Thanh Thi Nguyen

{"title":"Anti-atherosclerotic effects of Camellia chrysantha and Gynostemma pentaphyllum extracts mixture","authors":"Hong Hanh Nguyen, Ngan Hoang Nguyen, Tu Thanh Thi Nguyen","doi":"10.34172/jhp.2023.44843","DOIUrl":"https://doi.org/10.34172/jhp.2023.44843","url":null,"abstract":"Introduction: The combination Camellia chrysantha and Gynostemma pentaphyllum is used in Vietnam against hyperlipidemia. This study aims to evaluate the effects of the extract mixture of C. chrysantha and G. pentaphyllum in the atherosclerosis-induced rat model. Methods: Rats were administered with the extract mixture of C. chrysantha and G. pentaphyllum daily (7 and 14 g/kg/day) for 8 weeks upon the start of the study while they were simultaneously put on an atherosclerosis-induced diet. Blood samples were taken to examine the blood lipid indicators of the rats in the beginning, after 4 and 8 weeks of the study, respectively. After 8 weeks of treatment, the rats’ livers were removed to assess their overall health and the atherosclerosis patterns of their abdominal arteries. Results: The mixture of leaf extracts of C. chrysantha and G. pentaphyllum in the doses of 7 and 14 g/kg/day reduced blood lipid indices, including triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) levels and the atherogenic index (AI). Furthermore, this mixture also increased the levels of high-density lipoprotein cholesterol (HDL-C) in the blood, decreased the incidence of liver fat, and prevented the development of atherosclerotic lesions in the abdominal aorta in the rats. Conclusion: These results indicate that a mixture of C. chrysantha and G. pentaphyllum has a potential in preventing and treating atherosclerosis","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-cancer effects of the extracts of broad and spirale cultivars of Codiaeum variegatum (L.) Blume on MCF-7, HepG2, and HeLa cell lines Codiaeum variegatum（L. Blume）宽种和螺旋种提取物对 MCF-7、HepG2 和 HeLa 细胞系的抗癌作用

Journal of HerbMed Pharmacology Pub Date : 2023-08-10 DOI: 10.34172/jhp.2023.45002

Phanida Suphiratwanich, Benjaporn Buranrat, Supavadee Boontha

{"title":"Anti-cancer effects of the extracts of broad and spirale cultivars of Codiaeum variegatum (L.) Blume on MCF-7, HepG2, and HeLa cell lines","authors":"Phanida Suphiratwanich, Benjaporn Buranrat, Supavadee Boontha","doi":"10.34172/jhp.2023.45002","DOIUrl":"https://doi.org/10.34172/jhp.2023.45002","url":null,"abstract":"Introduction: Codiaeum variegatum (L.) Blume is a well-known ornamental foliage plant used as a vegetable in northern Thailand, and it is the source of numerous bioactive substances. This work explored the effects of leaf extracts of broad (BCE) and spirale (SCE) cultivars of C. variegatum on three cancer cells, including human breast, human liver, and human cervical cancer cells. Methods: Ethanolic plant extracts were prepared, and then, 2,2-diphenyl-1-picryhydrazyl (DPPH), ferrous iron chelating, and lipid peroxidation assays were used to examine the flavonoid and phenolic compounds. The proliferative inhibition, growth, and migration of MCF-7, HepG2, and HeLa cancer cells, as a result of exposure to the extracts, were investigated. The extracts were investigated for their anti-cancer activities using sulforhodamine B (SRB), clonogenic, and wound-healing methods. Results: The data demonstrated that BCE and SCE contained high phenolic compounds. However, both extracts showed inactive anti-oxidant activities. Both extracts had high cytotoxicity on three types of cancer cells in a dose- and time-dependent manner after 24-72 hours of incubation with IC50 values in a range of 208-830 µg/mL. Moreover, the prepared extracts of C. variegatum significantly inhibited colony-forming ability and cell migration on all types of cancer cells. Compared with BCE, SCE showed more potent anti-cancer activities. Conclusion: These findings revealed that SCE had higher anti-cancer activities on MCF-7, HepG2, and HeLa cancer cells than BCE. Consequently, the SCE might be used as an effective chemotherapeutic compound for the prevention and treatment of cancer.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical and anticandidal efficacy of Olea europaea leaf extract from different cultivars and seasonal variation 不同栽培品种油橄榄叶提取物的植物化学成分和抗念珠菌药效及季节变化

Journal of HerbMed Pharmacology Pub Date : 2023-08-10 DOI: 10.34172/jhp.2023.45000

Pedram Alimosazadeh, Mohd Sajjad Ahmad Khan, S. J. Sanei

{"title":"Phytochemical and anticandidal efficacy of Olea europaea leaf extract from different cultivars and seasonal variation","authors":"Pedram Alimosazadeh, Mohd Sajjad Ahmad Khan, S. J. Sanei","doi":"10.34172/jhp.2023.45000","DOIUrl":"https://doi.org/10.34172/jhp.2023.45000","url":null,"abstract":"Introduction: Olive (Olea europaea L.) is one of the important agricultural and medicinal tree species. This study aimed to assess the antifungal efficacy of olive leaf extract (OLE) obtained from different cultivars and during different seasons against Candida albicans strains. Methods: OLE was prepared using four olive cultivars (‘Koroneiki’, ‘Mission’, ‘Rowghani’, and ‘Zard’) obtained in two seasons (spring and autumn) from Golestan province, North of Iran. The phenolic and oleuropein contents of vegetative leaves were measured by colorimetric and HPLC techniques, respectively. The antifungal capacities of OLEs were tested by agar well diffusion and the minimal inhibitory concentration (MIC) was evaluated by micro-dilution assay. Results: The findings of our study showed that the total phenolic (27.45-88.16 mg g-1) and oleuropein (3.64-18.13 mg g-1) contents varied in leaf extracts, respectively. The highest amount was found in ‘Koroneiki’ and ‘Zard’ (spring leaves) and the lowest in ‘Mission’ cultivars (autumn leaves). The inhibition zones and MIC ranged from 1.92 to 15.41 mm and from 6.07 to 27.20 mg mL-1 based on C. albicans strains, respectively. Relationship between total phenolic content as an independent variable (X) and inhibition zones or MIC as dependent variables (Y) fitted polynomial curves. Conclusion: The present study highlighted the phytochemical and anti-candidal efficacy of OLE derived from olive cultivars or the seasons of harvested leaves against C. albicans strains. It is suggested that ‘Koroneiki’ and ‘Zard’ cultivars, especially during the spring season, could be exploited to isolate potential broad-spectrum antifungal drugs.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiofilm and antibacterial activities of lupinifolin in combination with protein synthesis inhibitors against methicillin-resistant Staphylococcus aureus 羽扇豆苷与蛋白质合成抑制剂联合对耐甲氧西林金黄色葡萄球菌的抗生物膜和抗菌活性

Journal of HerbMed Pharmacology Pub Date : 2023-08-10 DOI: 10.34172/jhp.2023.46056

Parichart Kwaengmuang, Koravich Chaiyawong, Todsapon Warong, Sakulrat Rattanakiat, P. Pulbutr

{"title":"Antibiofilm and antibacterial activities of lupinifolin in combination with protein synthesis inhibitors against methicillin-resistant Staphylococcus aureus","authors":"Parichart Kwaengmuang, Koravich Chaiyawong, Todsapon Warong, Sakulrat Rattanakiat, P. Pulbutr","doi":"10.34172/jhp.2023.46056","DOIUrl":"https://doi.org/10.34172/jhp.2023.46056","url":null,"abstract":"Introduction: Methicillin-resistant Staphylococcus aureus (MRSA)-derived biofilm formation is a crucial virulence factor, which essentially contributes to therapeutic challenges. This study aims to evaluate the antibiofilm and antibacterial formation activities of lupinifolin, a prenylated flavanone derived from Derris reticulata Craib. stem, in combination with protein synthesis inhibitors. Methods: The crystal violet biofilm formation assay was performed to determine the biofilm formation activity. The synergistic antibacterial activities were evaluated using the checkerboard and time-kill assays. Results: Lupinifolin and tetracycline significantly reduced MRSA biofilm formation with IC50 values of 15.32 ± 5.98 and 13.42 ± 5.90 µg/mL, respectively. On the contrary, the individual treatment of streptomycin and clindamycin tended to enhance biofilm formation. Lupinifolin at the sub-MIC of 8 µg/mL in combination with certain sub-MICs of tetracycline (8 and 16 µg/mL), streptomycin (16, 32, and 64 µg/mL), or clindamycin (4, 8, and 16 µg/mL) caused significant inhibitions against MRSA biofilm formation (P<0.05). The combination of lupinifolin and streptomycin exhibited a synergy (FIC index <0.625), confirmed in the time-kill assay. Conversely, the combination of lupinifolin and tetracycline or clindamycin resulted in no interaction (FIC indices of 1.0078 and <1.0156, respectively). Conclusion: The antibacterial synergy of lupinifolin and streptomycin possibly contributed to their antibiofilm-forming activity. However, the combinations of lupinifolin and tetracycline or clindamycin conceivably executed their antibiofilm activity directly against the MRSA biofilm formation process. These findings indicate a potential role for lupinifolin as an antibiofilm enhancer to diminish MRSA biofilm formation.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Focus on the ethnobotany of north Moroccan sage, false yellowhead, and carrot: insights into their pharmacological potential 聚焦摩洛哥北部鼠尾草、假黄头草和胡萝卜的民族植物学：对其药理潜力的见解

Journal of HerbMed Pharmacology Pub Date : 2023-08-10 DOI: 10.34172/jhp.2023.46047

Mohamed Jeddi, Zineb Benziane Ouaritini, Rachida Shita, K. Fikri-Benbrahim

{"title":"Focus on the ethnobotany of north Moroccan sage, false yellowhead, and carrot: insights into their pharmacological potential","authors":"Mohamed Jeddi, Zineb Benziane Ouaritini, Rachida Shita, K. Fikri-Benbrahim","doi":"10.34172/jhp.2023.46047","DOIUrl":"https://doi.org/10.34172/jhp.2023.46047","url":null,"abstract":"Introduction: Medicinal plants, including spontaneous or cultured herbaceous and forest products, represent an inexhaustible source of traditional and effective remedies thanks to their active major compounds. The present work consists of an ethnobotanical study of three species namely, Daucus carota, Dittrichia viscosa, and Salvia officinalis, commonly used in Taounate region (Northern Morocco) to treat various diseases. Methods: An ethnopharmacological survey was conducted in Taounate region during a period of three months from January to March 2022, using semi-structured individual interviews. Then, the collected data were analyzed statistically using Microsoft Office software \"Excel 2013\" and System Package for Social Sciences (SPSS). Results: Leaves, flowers, and stems were the most common parts used to prepare traditional remedies. Decoction, infusion, and cataplasm were the most used preparation methods, and the oral route was the most common method of administration for the studied plants. Moreover, the plants intervened in the treatment of digestive, genito-urinary, dermatological, neurological, and metabolic diseases. Conclusion: Information collected during this study shed light on the interesting know-how in traditional herbal medicine in the study area, and on the frequent use of medicinal plants as an alternative to synthetic drugs by the population of Taounate, to treat different diseases. Thereafter, the study’s results can constitute an important database for pharmacologists, phytochemists, toxicologists, and clinical researchers for the development of new drugs based on natural substances.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiparasitic and cytotoxicity effects of 7-hydroxy-4′- methoxy isoflavone against Leishmania major 7-hydroxy-4′- methoxy isoflavone 对大肠利什曼原虫的抗寄生和细胞毒性作用

Journal of HerbMed Pharmacology Pub Date : 2023-08-10 DOI: 10.34172/jhp.2023.44897

Mahdi Aghaei, Farhood Alizadegan, Yosra Raziani, Githa Kishore, Massumeh Saadatmand, Suja Ajoy Kumar

{"title":"Antiparasitic and cytotoxicity effects of 7-hydroxy-4′- methoxy isoflavone against Leishmania major","authors":"Mahdi Aghaei, Farhood Alizadegan, Yosra Raziani, Githa Kishore, Massumeh Saadatmand, Suja Ajoy Kumar","doi":"10.34172/jhp.2023.44897","DOIUrl":"https://doi.org/10.34172/jhp.2023.44897","url":null,"abstract":"Introduction: Leishmaniasis caused by Leishmania spp. is observed in most parts of the world. Although, glucantime, a pentavalent antimony compound, and other synthetic drugs are broadly applied for leishmaniasis therapy; however, the use of these synthetic agents has some limitations. Hence, this study was designed to assess the inhibiting effects of 7-hydroxy4′-methoxyisoflavone (7HMI) against promastigote and amastigote stages of Leishmania major in vitro. Methods: The MTT assay was applied to study the antileishmanial activity of 7HMI against promastigotes and its cytotoxicity effects on macrophage cells. The nitric oxide (NO) produced by the treated macrophage cells with 7HMI was also assessed. Results: 7HMI considerably (P<0.05) inhibited the growth rate of promastigotes and amastigotes stages. The 50% inhibitory concentrations of 7HMI and glucantim were 11.3 and 15.4 µg/mL for promastigote and amastigote, respectively. 7HMI, especially at 1/3 IC50 and 1/2 IC50 concentrations, considerably triggered the NO release. Conclusion: The current research findings reported the favorable antileishmanial effects of 7HMI against L. major with possible mechanisms such as reducing the infectivity rate of macrophage cells and provoking NO creation. Nevertheless, more research must be performed to clear its efficacy in animal model and then in human.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of Glycyrrhiza glabra L. root (licorice) extract against severe acute pancreatitis-induced acute lung injury via suppressing autophagy and inflammation 甘草提取物通过抑制自噬和炎症对重症急性胰腺炎所致急性肺损伤的保护作用

Journal of HerbMed Pharmacology Pub Date : 2023-08-10 DOI: 10.34172/jhp.2023.44699

N. EL-Rahmany, E. A. M. Sharaf

{"title":"Protective effect of Glycyrrhiza glabra L. root (licorice) extract against severe acute pancreatitis-induced acute lung injury via suppressing autophagy and inflammation","authors":"N. EL-Rahmany, E. A. M. Sharaf","doi":"10.34172/jhp.2023.44699","DOIUrl":"https://doi.org/10.34172/jhp.2023.44699","url":null,"abstract":"Introduction: Acute pancreatitis (AP) is an inflammatory disease with a high incidence of morbidity and mortality rate. The present study aimed to evaluate the protective effect of licorice extract administration on L-arginine-induced AP and associated lung tissue damage in rats. Methods: The experimental groups were the healthy control group (G1), L-arginine group (G2), licorice extract group (G3), and licorice extract +L-arginine; (protection group; G4). The protective effect of licorice extract was evaluated by measuring serum amylase and lipase, oxidative stress markers (malondialdehyde, nitric oxide, and myeloperoxidase), and inflammatory biomarkers levels (interleukin-6, tumor necrosis factor-alpha, toll-like receptor 4, and vascular cell adhesion molecule 1), as well as apoptosis assessment via caspase-3 activity and beclin-1 expression. Furthermore, an immunohistochemical assessment of heme oxygenase-1 (HO-1) and a histopathological examination of lung and pancreatic tissues were performed. Results: Licorice extract administration significantly reduced serum amylase, lipase, and inflammatory markers levels that pointed to the local and systemic inflammatory condition of AP induced by L-arginine. Moreover, the administration of licorice extract reversed the significant elevation in oxidative stress markers levels in the pancreas and lung tissues. Furthermore, licorice extract downregulated pancreatic gene expression of beclin-1 and caspase-3 which reversed dysregulated pancreatic autophagy. Conclusion: Licorice extract administration causes modulation of oxidative damage and systemic inflammation associated with acute pancreatic damage. Moreover, licorice extract markedly decreases the biochemical and histopathologic changes in AP, preserving the pancreatic and lung tissues through its antioxidant, anti-inflammatory, and antiapoptotic effects.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative study of the antibacterial and anti-inflammatory activities of the seed coat vs. seed kernel extracts from the plant Mangifera indica L. in inflammatory acne treatment 芒果种皮与种仁提取物在治疗炎症性痤疮中的抗菌消炎活性比较研究

Journal of HerbMed Pharmacology Pub Date : 2023-08-10 DOI: 10.34172/jhp.2023.48081

Ngoc Nha Thao Nguyen, Duc Linh Vo, Duy Khanh Dang, T. H. Huynh, Cao Thien Ha

{"title":"Comparative study of the antibacterial and anti-inflammatory activities of the seed coat vs. seed kernel extracts from the plant Mangifera indica L. in inflammatory acne treatment","authors":"Ngoc Nha Thao Nguyen, Duc Linh Vo, Duy Khanh Dang, T. H. Huynh, Cao Thien Ha","doi":"10.34172/jhp.2023.48081","DOIUrl":"https://doi.org/10.34172/jhp.2023.48081","url":null,"abstract":"Introduction: The present study was developed to investigate and compare the extraction yield, the contents of polyphenols and mangiferin, the activities against Propionibacterium acnes, Staphylococcus aureus, and Escherichia coli bacteria, and the anti-inflammatory activities of ethanol extracts of mango seed kernels vs. seed coats. Methods: Mango seed kernels and seed coats were extracted using ethanol as the solvent and tested against microorganisms using the disc diffusion method. The minimum inhibitory concentration (MIC) levels of extracts were determined by the agar dilution method. The anti-inflammatory activities were assessed both in vitro and in vivo by albumin denaturation method and carrageenan-induced paw edema test, respectively. Results: Both extracts yielded high contents of mangiferin and phenolic compounds. The antibacterial activities of both extracts showed inhibition of the tested microorganisms Propionibacterium acnes and Staphylococcus aureus but not Escherichia coli. Seed kernel extract (0.2 g/kg) reduced paw edema by 44.8% at 3 hours after λ-carrageenan administration. Meanwhile, 0.5 g/kg seed coat extract reduced paw edema less than the seed kernel extract (23.1% vs. 44.8%). Mango seed kernel extract, mango seed coat extract, and diclofenac sodium displayed concentration-dependent inhibition of heat-induced protein denaturation with IC50 values of 137.23 μg/mL, 292.12 μg/mL, and 6.64 μg/mL, respectively. Conclusion: The obtained results confirmed the antibacterial and anti-inflammatory potential of mango seed kernel and seed coat extracts. The mango seed kernel extract was proven to be more effective than the mango seed coat extract and thus can be used in cosmetics as an anti-inflammatory and antibacterial agent.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"374 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical profiling, pharmacology prediction, and molecular docking study of Chromolaena odorata extract against multiple target proteins in wound healing Chromolaena odorata 提取物针对伤口愈合中多种靶蛋白的植物化学成分分析、药理学预测和分子对接研究

Journal of HerbMed Pharmacology Pub Date : 2023-08-10 DOI: 10.34172/jhp.2023.44672

Nur ‘Ainun Mokhtar, Fatahiya Mohamed Tap, Nur Hannani Ahmad Rozani, N. B. Ahmad Khairudin, R. Rasit Ali

{"title":"Phytochemical profiling, pharmacology prediction, and molecular docking study of Chromolaena odorata extract against multiple target proteins in wound healing","authors":"Nur ‘Ainun Mokhtar, Fatahiya Mohamed Tap, Nur Hannani Ahmad Rozani, N. B. Ahmad Khairudin, R. Rasit Ali","doi":"10.34172/jhp.2023.44672","DOIUrl":"https://doi.org/10.34172/jhp.2023.44672","url":null,"abstract":"Introduction: Wounds have a significant influence on socioeconomic and the quality of life. Many attempts have been taken to produce advanced wound dressing to fulfill demands. The incorporation of natural therapeutics like medicinal plants in wound dressings is currently popular. However, several medications have failed to enter the market due to inadequate pharmacokinetics data. Computer-aided tools are now available as advanced drug discovery methods, which can be used to screen pharmaceuticals from phytochemicals found in various medicinal plants. This study aims to evaluate the phytoconstituents of Chromolaena odorata extract and its pharmacological potential as a wound-healing agent. Methods: Phytoconstituents from C. odorata were identified using qualitative screening methods and gas chromatography-mass spectrometry (GC-MS), and their mechanistic properties were assessed using molecular docking and SwissADME tools. Results: Current works revealed that the topmost phytoconstituents in C. odorata were phytol (49.83%), hexadecanoic acid ethyl ester (9.40%), linolenic acid (8.07%), and squalene (3.53%). Through SwissADME analysis, all four topmost compounds obeyed Lipinski’s Rule of 5. In silico molecular docking study of these top phytoconstituents against several protein targets involved in wound healing revealed that squalene had the highest binding affinity to GSK3-β (-6.8 kJ/ mol), MMP-9 (-7.4 kJ/mol), and COX-2 (-8.6 kJ/mol) as compared to other ligands (phytol, linolenic acid, and hexadecenoic acid ethyl ester). Conclusion: These findings suggest that the most prominent compound that contributes to C. odorata’s wound healing capacity is squalene and the incorporation of C. odorata in potential wound dressing formulation is justified.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wound healing and antimicrobial activities of a spray gel of banana (Musa paradisiaca L.) peel extract in rabbit (Oryctolagus cuniculus) models 香蕉（Musa paradisiaca L.）果皮提取物喷雾凝胶在兔子（Oryctolagus cuniculus）模型中的伤口愈合和抗菌活性

Journal of HerbMed Pharmacology Pub Date : 2023-08-10 DOI: 10.34172/jhp.2023.48080

R. Rizka, Yuandani Yuandani, S. Sumaiyah

{"title":"Wound healing and antimicrobial activities of a spray gel of banana (Musa paradisiaca L.) peel extract in rabbit (Oryctolagus cuniculus) models","authors":"R. Rizka, Yuandani Yuandani, S. Sumaiyah","doi":"10.34172/jhp.2023.48080","DOIUrl":"https://doi.org/10.34172/jhp.2023.48080","url":null,"abstract":"Introduction: Musa paradisiaca peel has inhibited microbial growth and enhanced wound healing in animal models. However, the study on its effect as a dosage form is lacking. In the present study, the antimicrobial and wound-healing effects of a spray gel of M. paradisiaca peel extracts were evaluated in rabbits. Methods: The antimicrobial and wound healing activities of a spray gel were tested at different concentrations (10%, 15%, and 20%) of banana peel extract, categorized as low concentration (SGL), medium concentration (SGM), and high concentration (SGH) groups, respectively. The antimicrobial effects against Escherichia coli and Staphylococcus aureus were investigated by measuring inhibition zone diameters. Burns were inflicted on the back area of rabbits using hot steel. Macroscopic and microscopic examinations were performed. Results: The spray gel containing banana peel extract exhibited inhibition zone diameters of 14.2 ± 0.38 mm and 14.6 ± 0.21 mm against Escherichia coli and Staphylococcus aureus, respectively. SGH showed the strongest wound-healing activity of all the samples, which was comparable with bioplacenton (BG) as a positive control. The wounds healed on days 16, 16, 20, and 22 for bioplacenton, SGH, SGM, and SGL, respectively. There was a significant difference (P<0.05) in collagen density and epidermal thickness between the treatment groups and the negative control (1.2 % sodium carboxymethyl cellulose (Na-CMC)). Conclusion: The result indicates that the spray gel of M. paradisiaca peel ethanolic extract possesses antimicrobial and wound-healing activities, emphasizing its potential to be developed as a wound healing agent.","PeriodicalId":15934,"journal":{"name":"Journal of HerbMed Pharmacology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0