Journal of Genetics最新文献

{"title":"Characters of the <i>MOCA</i> family in wheat and <i>TaMOCA1</i> function in salt stress tolerance.","authors":"Yuxiang Qin, Ping Cui, Bao Zhang, Yuning Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p><i>MOCA1</i> encodes the last key glucuronosyltransferase for ionic stress sensor glycosyl inositol phosphoryl-ceramide (GIPCs) biosynthesis in <i>Arabidopsis</i>, which indicates that the <i>MOCA</i> gene family play important role in plant tolerance to salt stress. However, the isolation and function of <i>MOCAs</i> in staple crops have not been reported and the downstream targets of <i>MOCAs</i> in salt stress tolerance signalling pathway are not clear. In this study, we identified 110 <i>MOCA</i> genes in wheat which were classified into five clades and they differed in gene structure, protein length, conserved motifs and expression profiles in different tissues and under salt stress. <i>TaMOCA1</i> was selected for further functional study in response to salt stress. <i>TaMOCA1</i> was rapidly induced by NaCl treatment. The 35S::<i>TaMOCA1-GFP</i> construction showed the cell nucleus and cytoplasm location in wheat protoplast. <i>TaMOCA1</i> over-expressing <i>Arabidopsis</i> seedlings formed longer primary roots and more lateral roots than the wild type ones under 50 mM NaCl treatment. The over-expressing <i>Arabidopsis</i> had higher expression levels of <i>HKT1</i>, but lower expression levels of <i>NHX1</i> and <i>SOS</i> genes than the wild type. Also, the transgenic plants had higher SOD activity and lower MDA content than the wild <i>Arabidopsis</i> seedling under salt stress. These results may indicate that <i>TaMOCA1</i> increases salt stress tolerance through decreasing Na⁺ loading from the xylem parenchyma cells to the xylem via <i>SOS1</i> and <i>HKT1</i>, hence lowering root-to-shoot delivery of Na? and superior antioxidant ability. All these results lay a foundation for further functional study of <i>MOCAs</i> in wheat.</p>","PeriodicalId":15907,"journal":{"name":"Journal of Genetics","volume":"103 ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139912763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient assembly of a synthetic attenuated SARS-CoV-2 genome in <i>Saccharomyces cerevisiae</i> using multi-copy yeast vectors.","authors":"Abhishek Kumar Singh, Harsh Goar, Nikita Vashist, Prakash Sinha, Anand Kumar Bachhawat","doi":"","DOIUrl":"","url":null,"abstract":"<p><p><i>Saccharomyces cerevisiae</i> has been demonstrated to be an excellent platform for the multi-fragment assembly of large DNA constructs through its powerful homologous recombination ability. These assemblies have invariably used the stable centromeric single copy vectors. However, many applications of these assembled genomes would benefit from assembly in a higher copy number vector for improved downstream extraction of intact genomes from the yeast. A review of the literature revealed that large multi-fragment assemblies did not appear to have been attempted in multicopy vectors. Therefore, we devised a toolkit that would enable one to seamlessly transition with the same assembling fragments between a single copy and a multicopy vector. We evaluated the assembly of a 28 kb attenuated SARSCoV- 2 genome (lacking the N gene) from 10 fragments in both single copy and multicopy vector systems. Our results reveal that assembly was comparably efficient in the two vector systems. The findings should add to the synthetic biology toolkit of <i>S. cerevisiae</i> and should enable researchers to utilize any of these vector systems depending on their downstream applications.</p>","PeriodicalId":15907,"journal":{"name":"Journal of Genetics","volume":"103 ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139912764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction of genomic DNA for sequencing from snail <i>Helix lucorum</i>.","authors":"Dmitry Panteleev, Anastasia Sadova, Galina Pavlova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Genomic studies make it possible to breakthrough in many fields such as biochemistry, physiology, phylogenetics, etc., though\u0000they are unworkable without sequences of genomic DNA of an organism. The terrestrial mollusks’ genomes would benefit gastropod\u0000biology investigations, that are unavailable so far due to problems in DNA integrity and quality after the isolation procedures. Here we\u0000describe a fast and handy protocol for genomic DNA extraction from the tissues of <i>Helix lucorum</i>, which allows to yield high-quality\u0000samples applicable for downstream analysis such as high-throughput DNA sequencing. Troubleshooting revealed the nuclease activity of\u0000snail tissue lysate, which may be avoided by heating the lysate and decreasing the incubation time.</p>","PeriodicalId":15907,"journal":{"name":"Journal of Genetics","volume":"103 ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141237463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial control region sequences show high genetic connectivity in the brownstripe snapper, <i>Lutjanus vitta</i> (Quoy and Gaimard, 1824) from the east coast of Peninsular Malaysia.","authors":"Ahasan Habib, Nor Athirah Husna Ahmad, Nur Asma Ariffin, Ying Giat Seah, M M Mahbub Alam, Tun Nurul Aimi Mat Jaafar, Nur Fadli, Siti Azizah Mohd Nor, Md Moshiur Rahman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Brownstripe Snapper, <i>Lutjanus vitta</i> (Quoy and Gaimard, 1824) is a commercially important snapper extensively caught in Malaysia. We examined genetic diversity, population connectivity, and historical demographics of the <i>L. vitta</i>, off the eastern coast of peninsular Malaysia based on an 817 bp region of the mtDNA control region sequences. Maximum likelihood gene trees demonstrated that the populations under study had limited structuring and formed a single panmictic population that lacks support for internal clades. AMOVA and population pairwise Φ_ST values indicated high genetic exchange between the study areas. A high level of haplotype diversity (0.956-1.000) with low nucleotide diversity (0.008-0.014) indicates a recent expansion of <i>L. vitta</i> populations. However, both neutrality and goodness of fit tests revealed nonsignificant values. These data reflect a recent demographic expansion, which the Bayesian skyline plot estimates population expansion at 44 Kya. The absence of genetic differentiation can be attributed to spawning patterns, dispersal of egg and planktonic larvae, and the absence of physical barriers, which are typical of other <i>Lutjanus</i> species. The current findings could initiate efficient management strategies for <i>L. vitta</i> along Malaysia and other nearby nations that share the same waterways.</p>","PeriodicalId":15907,"journal":{"name":"Journal of Genetics","volume":"103 ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of specific molecular markers for medicinal peony (<i>Paeonia lactiflora</i>) with double flower.","authors":"Jinqiu Liao, Shuai Zhang, Qunqun Yang, Zhenge Han, Xuexue Deng, Ruiwu Yang, Yuanyuan Jiang, Li Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In China, medicinal <i>Paeonia lactiflora</i> with double flowers (DFs) does not produce seeds, yet it possesses significantly higher paeoniflorin content compared with its single-flowered counterpart. The propagation of medicinal <i>P. lactiflora</i> with DFs relies solely on rhizomes. However, due to economic motivations, the rhizomes of medicinal <i>P. lactiflora</i> with single flowers (SFs) are often mixed with those of medicinal <i>P. lactiflora</i> with DFs. This practice results in a mixed population and a subsequent decline in quality. To ensure the quality of medicinal <i>P. lactiflora</i> with DFs, it is essential to develop specific molecular markers for its identification and breeding. The genetic diversity of 30 populations from six species in sect. <i>Paeonia</i> was studied using inter-simple sequence repeat (ISSR) analysis. Specific bands in medicinal <i>P. lactiflora</i> with DFs and medicinal <i>P. lactiflora</i> with SFs were cloned and utilized as distinctive molecular markers for their identification. A total of 244 polymorphic bands were identified from 18 primers. Among these primers, UBC844 displayed the highest genetic diversity (<i>N</i>_e = 1.37, <i>h</i> = 0.23, <i>I</i> = 0.36). Based on the UPGMA and PCA analyses, all species were classified into three clusters. Medicinal <i>P. lactiflora</i> with SFs showed closer proximity to the wild-type species of <i>Paeonia</i>, while it was further apart from medicinal <i>P. lactiflora</i> with DFs. The specific band amplified by UBC836-3 (~431 bp) was exclusive to medicinal <i>P. lactiflora</i> with DFs, while the band amplified by UBC842-7 (~341 bp) was specific to medicinal <i>P. lactiflora</i> with SFs. There are significant genomic disparities between medicinal <i>P. lactiflora</i> with SFs and DFs. Consequently, stable and specific sequence characterized amplified region markers (UBC836-3 and UBC842-7) have been established to effectively differentiate between the two types. This development will substantially enhance the quality and efficiency of medicinal <i>P. lactiflora</i>, thus supporting the growth of the industry. By utilizing these specific molecular markers, breeding experts can select parent plants and shorten the cultivation cycle for new medicinal peony varieties.</p>","PeriodicalId":15907,"journal":{"name":"Journal of Genetics","volume":"103 ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of modification of DNA interference on myostatin gene expression in mice","authors":"Mitra Riasi, Sina Mozaffari-Jovin, A. Javadmanesh","doi":"10.1007/s12041-023-01453-7","DOIUrl":"https://doi.org/10.1007/s12041-023-01453-7","url":null,"abstract":"","PeriodicalId":15907,"journal":{"name":"Journal of Genetics","volume":"51 18","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138946323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fly clock, my clock, and lamin B receptor","authors":"D. P. Kasbekar","doi":"10.1007/s12041-023-01452-8","DOIUrl":"https://doi.org/10.1007/s12041-023-01452-8","url":null,"abstract":"","PeriodicalId":15907,"journal":{"name":"Journal of Genetics","volume":"52 11","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138951527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why are you hitting yourself? Whole-exome sequencing diagnosis of monogenic autoimmunity","authors":"Lina M. Castano-Jaramillo, Francisco Rivas Larrauri, Selma C. Scheffler-Mendoza, Alonso Gutierrez-Hernandez, Juan Carlos Bustamante Ogando, Paulina Colin, Margarita Ortega Cisneros, Sandra Rajme-López, Edgar Alejandro Medina-Torres, Laura Berron Ruiz, Ana Luisa Rodriguez-Lozano, Sara Elva Espinosa Padilla, Marco Antonio Yamazaki-Nakashimada, Saul O. Lugo Reyes","doi":"10.1007/s12041-023-01450-w","DOIUrl":"https://doi.org/10.1007/s12041-023-01450-w","url":null,"abstract":"<p>Inborn errors of immunity may present with autoimmunity and autoinflammation as hallmark clinical manifestations. We aimed to identify the potential monogenic causes of autoimmune disorders in 26 patients from a pediatric reference hospital in Mexico through whole-exome sequencing. We specifically selected patients with a family history of autoimmune diseases, early-onset symptoms, and difficult-to-control autoimmune disorders or autoimmunity associated with infection predisposition. We identified the genetic variants that were compatible with the patients' phenotype in 54% of the patients. Autoimmune diseases are often caused by a combination of genetic factors, but cases that appear at a young age are resistant to treatment or occur in clusters, as well as the presence of autoimmune symptoms alongside infectious diseases should raise suspicion for an underlying inborn error of immunity.</p>","PeriodicalId":15907,"journal":{"name":"Journal of Genetics","volume":"355 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138506362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity analysis of volunteer wheat based on SSR markers","authors":"Wangcang Su, Hongle Xu, Lanlan Sun, Chuantao Lu, Renhai Wu","doi":"10.1007/s12041-023-01451-9","DOIUrl":"https://doi.org/10.1007/s12041-023-01451-9","url":null,"abstract":"<p>Volunteer wheat is a kind of wheat with weed characteristics, distributed widely in the main wheat-producing areas of China. It seriously damages the yield and quality of cultivated wheat. To study the genetic diversity and population structure within and between volunteer wheat and cultivated wheat (<i>Triticum aestivum</i> L.), 195 volunteer wheat seeds and 29 cultivated wheat seeds were analysed based on 16 pairs of highly-polymorphic microsatellite simple sequence repeats (SSR) primers and a microchip capillary electrophoresis (MCE) detection system. A total of 110 polymorphic alleles were detected by MCE with each pair of primers identifying 2–15 alleles with an average of 6.875 alleles. The polymorphic information content (PIC) ranged from 0.1089 to 0.7843, with an average of 0.5613. Genetic diversity arguments from 224 samples showed that the volunteer wheat was more varied than cultivated wheat. Based on the SSR information, the 224 samples were classified into seven groups, which corresponded to the volunteer wheats and cultivated wheats through principal coordinates analysis (PCA). We propose that the volunteer wheat and cultivated wheat have rather distant phylogenetic relationships. Hence, it is important for wheat breeding to study the genetic relationship between volunteer wheat and cultivated wheat.</p>","PeriodicalId":15907,"journal":{"name":"Journal of Genetics","volume":"352 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138506363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"olf413, a putative octopamine biosynthesis pathway gene is required for negative geotactic motor function in Drosophila melanogaster","authors":"Ravindrakumar Ramya, Baragur Venkatanarayanasetty Shyamala","doi":"10.1007/s12041-023-01449-3","DOIUrl":"https://doi.org/10.1007/s12041-023-01449-3","url":null,"abstract":"<p><i>Olf413</i> gene annotated as CG12673 in the genome of <i>Drosophila melanogaster</i> has been predicted to code for a protein with putative function in octopamine biosynthesis. The expression pattern and the biological function of the gene awaits investigation. The present study is the first report where we describe its expression in the late pupal brain as depicted by enhancer-driven green fluorescent protein (GFP) expression. With experiments using loss of function <i>olf413</i> mutant flies, we have demonstrated that <i>olf413</i> function is essential for motor activity, gravity sensing and spatial balancing of the body against gravity during climbing.</p>","PeriodicalId":15907,"journal":{"name":"Journal of Genetics","volume":"343 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138506352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}