{"title":"Impact of selenium supplementation on antioxidant enzyme activity and liver gene expression in juvenile pangasius catfish (Pangasius pangasius).","authors":"Albin Jemila Thangarani, Nathan Felix, Arul Victor Suresh, Seerappalli Aran Shanmugam, Ayyathurai Kathirvelpandian, Eswaran Suresh, Rajagopal Ramya, Amit Ranjan, Elangovan Prabu, Thodhal Yoganandham Suman","doi":"10.1111/jfb.16036","DOIUrl":"https://doi.org/10.1111/jfb.16036","url":null,"abstract":"<p><p>This study examined the effect of dietary selenium (Se) fortification on growth efficiency, antioxidant status, and liver gene expression in juvenile pangasius catfish. Sodium selenite was incorporated into a basal diet at incremental levels of 0-2.0 mg Se/kg. This resulted in final Se concentrations of 0.63, 0.85, 1.15, 1.68, 2.10, and 2.65 mg Se/kg, respectively. A total of 15 fish, with an average individual weight of 5.6 ± 0.1 g, were subjected to one of six dietary treatments and fed in triplicate over a 60-day period. A significant dose-related enhancement in growth performance was observed, with the optimal dietary Se requirement estimated at 1.75 mg Se/kg. Se-fortified diet significantly increased the activities of digestive enzymes (lipase and protease) as well as antioxidant enzymes, including catalase, glutathione peroxidase, and superoxide dismutase in the liver. Notably, the group fed 1.68 mg Se/kg displayed the highest levels of these enzymes. Additionally, selenium supplementation resulted in an upregulation of the relative expression of insulin-like growth factor 1X1 and 1X2 in the liver, highlighting its pivotal role in growth regulation. Blood parameters showed significant improvements in mean corpuscular hemoglobin and hemoglobin levels with Se supplementation. Proximate analysis showed increasing Se intake resulted in a significant rise in muscle protein content. In pangasius catfish, our results indicate that dietary selenium supplementation can enhance growth efficiency, antioxidant capacity, and hepatic gene expression.</p>","PeriodicalId":15794,"journal":{"name":"Journal of fish biology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142921704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ugo E Pazzaglia, Marcella Reguzzoni, Genciana Terova, Fabrizio Serena, Cecilia Mancusi, Guido Zarattini, Piero A Zecca
{"title":"Growth and mechanical correlations of calcified cartilage in Batoidea: A histomorphological study using the Raja asterias model.","authors":"Ugo E Pazzaglia, Marcella Reguzzoni, Genciana Terova, Fabrizio Serena, Cecilia Mancusi, Guido Zarattini, Piero A Zecca","doi":"10.1111/jfb.16037","DOIUrl":"https://doi.org/10.1111/jfb.16037","url":null,"abstract":"<p><p>This study investigates the growth and calcification of the appendicular skeleton in Raja asterias (Delaroche, 1809), a member of the Batoidea, to explore the relationship between histomorphology and the mechanics of batoid locomotion within the water column. Although much prior research has focused on the \"tessellated pattern\" in these fishes, the variable structure of the appendicular skeleton provides fresh insights into the understudied interplay between skeletal histomorphology and the mechanical functions of Batoidea fins. The shape and initial growth of fin cartilage are influenced by the orientation of chondrocyte mitoses prior to mineral deposition, with subsequent calcification playing a pivotal role in shaping skeletal architecture. This study documents two distinct growth patterns: \"crustal\" and \"catenated.\" The crustal pattern is predominantly observed in larger skeletal elements, such as the central body structures (skull, rostrum, and jaws), girdles, pterygia, and compound radials, whereas fin radials follow the catenated growth pattern. Notably, early-stage chondrichthyan cartilage shares similarities with mammalian metaphyseal growth plate cartilage, though in chondrichthyans, the calcified matrix is not resorbed or replaced by bone. Additionally, a previously unrecognized calcification pattern is identified in the pelvic-fin radials of R. asterias, indicating that the mechanical demands of locomotion in the water column may have driven the evolution of variable fin flexibility in Batoidea. This flexibility is achieved through joint mobility (diarthroses and amphiarthroses), specialized fin structures, and the distinct calcification patterns of the pectoral and pelvic fins.</p>","PeriodicalId":15794,"journal":{"name":"Journal of fish biology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142921699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adrian Indermaur, Frederic D B Schedel, Fabrizia Ronco
{"title":"Morphological diversity of the genus Telmatochromis from the Lake Tanganyika drainage with the description of a new riverine species and the generic reassignment of the Malagarasi River lamprologine.","authors":"Adrian Indermaur, Frederic D B Schedel, Fabrizia Ronco","doi":"10.1111/jfb.16042","DOIUrl":"https://doi.org/10.1111/jfb.16042","url":null,"abstract":"<p><p>The lamprologine cichlid genus Telmatochromis was long considered primarily lacustrine and endemic to Lake Tanganyika until an undescribed Telmatochromis species was reported from the Lufubu River (Lake Tanganyika drainage, Zambia). A phylogenomic study in 2021 confirmed the association of Telmatochromis sp. \"lufubu\" with Telmatochromis along with another riverine species, Neolamprologus devosi (Malagarasi drainage, Tanzania). Here, we quantify the morphological diversity of the genus Telmatochromis and the two associated riverine species using a multivariate dataset combining geometric and classical morphometrics, as well as meristics. We identify three distinct morphological clusters: the \"Telmatochromis vittatus complex\" with highly elongated bodies and short heads, the \"Telmatochromis temporalis complex\" with deeper bodies, and the two riverine species with intermediate body elongation and large heads. Further, we formally describe the species endemic to the lower Lufubu River as Telmatochromis salzburgeri sp. nov. and reassign N. devosi to Telmatochromis. Telmatochromis devosi comb. nov. differs from all congeners by the absence of bi- and tricuspid teeth in the inner tooth rows of the oral dentition. T. salzburgeri sp. nov. can be distinguished from all other Telmatochromis species by a prominent orange stripe along the base of the dorsal fin and from T. devosi comb. nov. by the relatively smaller size of the lower pharyngeal jaw. Both riverine species differ from all lacustrine Telmatochromis by a lower number of dorsal-fin spines. Additionally, the riverine species can be distinguished from the T. vittatus complex by having larger heads and longer oral jaws, and from the T. temporalis complex by their lower relative body depth. With the inclusion of new riverine members, the genus Telmatochromis is revealed to be more morphologically and ecologically diverse than previously recognized.</p>","PeriodicalId":15794,"journal":{"name":"Journal of fish biology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effects of handling on stress response markers in a reef fish model for aquaculture development, the brassy chub, Kyphosus vaigiensis.","authors":"Reilly S Merlo, Bradley K Fox, Andre P Seale","doi":"10.1111/jfb.16044","DOIUrl":"https://doi.org/10.1111/jfb.16044","url":null,"abstract":"<p><p>With the expanding global population, interest has increased in the sustainable aquaculture development of indigenous fishes. In Hawai'i, the brassy chub, Kyphosus vaigiensis, has drawn interest as a candidate species for production. However, little is known about its resilience in aquaculture settings where fish are exposed to acute, husbandry-related stressors. To address the response of brassy chub to handling stress, we characterized the effects of simulated netting and confinement stress on physiological parameters indicative of the alarm and resistance phases of the stress response over a 24-h time course. Specifically, we measured plasma osmolality and glucose, mucous cortisol, hepatic activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPX), and hepatic lipid peroxidation (LPO). Plasma osmolality in stressed fish was increased relative to controls at 1 and 6 h. Mucous cortisol levels were transiently increased in stressed fish at 1 h following handling stress. In stressed fish, plasma glucose was generally increased from 1 h to 24 h relative to controls. By contrast, SOD activity decreased in stressed fish at 6 and 12 h, returning to control levels at 24 h; GPX activity and LPO were unaffected by the handling stressor. The transient and modest responses of mucous cortisol and antioxidant enzyme activity indicate the recovery of brassy chub from handling stress. Moreover, the sustained increase in plasma glucose and lack of change in LPO suggest mobilization of energy and long-term resiliency toward handling stressors.</p>","PeriodicalId":15794,"journal":{"name":"Journal of fish biology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Julia Saltzman, Cliff Hawkins, Catherine C Macdonald
{"title":"The silver king in the Magic City: Observation of Atlantic tarpon Megalops atlanticus aggregation off Miami, Florida.","authors":"Julia Saltzman, Cliff Hawkins, Catherine C Macdonald","doi":"10.1111/jfb.16035","DOIUrl":"https://doi.org/10.1111/jfb.16035","url":null,"abstract":"<p><p>Due to the logistical and financial challenges in studying migratory marine species, there is relatively limited knowledge of the reproductive biology, behavior, and habitat use of many ecologically important marine megafauna species, including the Atlantic tarpon Megalops atlanticus. Here, we present a novel observation using consumer-grade aerial drones to observe, quantify the scale of, and classify behaviors within a previously unreported tarpon aggregation (N = 182) over the course of a 2-day fish aggregation event. After the event, we analysed and compared observed behaviors (e.g., cruising and clustering) with those of other fish species with well-documented reproductive behaviors, revealing behaviors potentially consistent with courtship, leading us to believe this to be a possible spawning aggregation or aggregation of reproductive/migratory significance. This aggregation occurred in highly altered and urbanized habitat off the coast of South Florida, during the winter, and outside of tarpon's previously reported spring and summer spawning season. Although few studies to date have used drones to monitor teleosts, this study reinforces their potential value as a tool for monitoring fish populations, behavior, and movement.</p>","PeriodicalId":15794,"journal":{"name":"Journal of fish biology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Claudia Junge, Keno Ferter, C Antonia Klöcker, Otte Bjelland, Jon Albretsen, Robert J Lennox, Finn Økland, Romaric Jac, Hector Andrade, Ole Thomas Albert
{"title":"Tag attachment innovation on spurdog (Squalus acanthias) reveals year-round coastal association of pregnant females in northeastern Atlantic waters.","authors":"Claudia Junge, Keno Ferter, C Antonia Klöcker, Otte Bjelland, Jon Albretsen, Robert J Lennox, Finn Økland, Romaric Jac, Hector Andrade, Ole Thomas Albert","doi":"10.1111/jfb.16000","DOIUrl":"https://doi.org/10.1111/jfb.16000","url":null,"abstract":"<p><p>The spurdog (Squalus acanthias Linnaeus, 1758) is a globally distributed squaliform shark that has historically been overfished but is now recovering in the northeast Atlantic. Data series on spurdog movement and habitat use have been somewhat limited to research surveys due to challenges associated with electronic tagging. Here, we offer a revised attachment method for externally attached pop-up satellite archival tags that was successful in long-term deployments on pregnant females. Twenty-one spurdogs were tagged in two fjord systems in western Norway for an average of 243 days and provided new details about their behaviour, demonstrating affinity for coastal habitat based on the pop-up locations and recovery positions of the tags (84% within 40 km from tagging location), and depth-temperature profiles. It is likely that parturition therefore occurs in these coastal waters, making them important to the life cycle of this species. The realized depth niche of tagged individuals suggested that surveys may miss sharks if they do not cover the full water column because the sharks used large parts of the water column and spent much time in shallower waters, albeit with seasonal variations (deeper and shallower in winter and summer, respectively). Adoption of this tagging method and combination with movement data from acoustic transmitters will help to better resolve the behaviour of this species as it transitions from a species at risk to a managed fishery. Such studies will provide a more comprehensive understanding of the species' habitat requirements that will empower better informed protections against a return to the red list of threatened species.</p>","PeriodicalId":15794,"journal":{"name":"Journal of fish biology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jurandir Joaquim Bernardes Junior, Janaína Dos Santos Pedron, Renata Maria Guereschi, Rafael José Gonçalves Dias, Alex Pires de Oliveira Nuñer
{"title":"Pimelodus maculatus abundance in the tailrace of the Machadinho hydroelectric power plant during drought conditions.","authors":"Jurandir Joaquim Bernardes Junior, Janaína Dos Santos Pedron, Renata Maria Guereschi, Rafael José Gonçalves Dias, Alex Pires de Oliveira Nuñer","doi":"10.1111/jfb.16046","DOIUrl":"https://doi.org/10.1111/jfb.16046","url":null,"abstract":"<p><p>Fish presence in tailraces is a remarkable management concern in operating hydroelectric power plants (HPP) in Brazilian rivers. Fish get blocked during upstream migration for spawning, and large shoals can last for days in the tailrace; however, the circumstances that attract fish throughout the year to this region are uncertain, especially during drought conditions. To unravel this uncertainty, we modeled the relationship between Pimelodus maculatus abundance and environmental and operational variables (N = 26) in monthly fishing campaigns at the Machadinho HPP (N = 18). A generalized additive model was fitted to fish abundance in which water conductivity, 30-day accumulated precipitation, reservoir inflow, and seasonality explained 93% of the deviance. It was found that abundance decreased as water conductivity increased, but abundance was higher in summer. However, winter showed the highest abundance in the entire rainfall range with peaks between 130 and 150 mm and above 250 mm. An increase in reservoir inflow decreased fish abundance. During droughts, our findings suggest that the powerhouse foundations, particularly the draft tube, may serve as refugia to P. maculatus.</p>","PeriodicalId":15794,"journal":{"name":"Journal of fish biology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Malik Haris Ali, Ying-Ping Huang, David Johnson, Wei-Ming Li, Xi Yuan
{"title":"Effects of pre-experimental fasting and sheltering on the swimming performance and oxygen consumption of juvenile grass carp (Ctenopharyngodon idella).","authors":"Malik Haris Ali, Ying-Ping Huang, David Johnson, Wei-Ming Li, Xi Yuan","doi":"10.1111/jfb.16034","DOIUrl":"https://doi.org/10.1111/jfb.16034","url":null,"abstract":"<p><p>This study investigated the effects of pre-experimental fasting for 2, 7, and 14 days under both unsheltered (12 h dark-12 h light) and sheltered conditions on the critical swimming speed (U<sub>crit</sub>) and oxygen consumption (MO<sub>2</sub>) of juvenile grass carp, using a modified swim respirometer. Key findings include (1) the U<sub>crit</sub> of test fish decreased significantly, by 28%, after fasting for 14 days and sheltering intensified the impact to a 43% decrease, and (2) fish anaerobic capacity decreased after 7 days but increased after 14 days, and was enhanced by sheltering conditions. These findings are important as they indicate that fasting and sheltering can impair grass carp survival and disrupt river ecosystem balance, highlighting the need for habitat conservation.</p>","PeriodicalId":15794,"journal":{"name":"Journal of fish biology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
James Hinchcliffe, Jonathan A C Roques, Andreas Ekström, Ida Hedén, Kristina Sundell, Henrik Sundh, Erik Sandblom, Björn Thrandur Björnsson, Elisabeth Jönsson
{"title":"Insights into thermal sensitivity: Effects of elevated temperature on growth, metabolic rate, and stress responses in Atlantic wolffish (Anarhichas lupus).","authors":"James Hinchcliffe, Jonathan A C Roques, Andreas Ekström, Ida Hedén, Kristina Sundell, Henrik Sundh, Erik Sandblom, Björn Thrandur Björnsson, Elisabeth Jönsson","doi":"10.1111/jfb.16017","DOIUrl":"https://doi.org/10.1111/jfb.16017","url":null,"abstract":"<p><p>The Atlantic wolffish (Anarhichas lupus) is a cold-water fish with potential for aquaculture diversification. To unveil the mechanisms underlying the compromised growth in Atlantic wolffish when reared at higher temperatures, we investigated the relationship between temperature, growth rate, aerobic capacity, stress biomarkers, and gut barrier function. Juveniles acclimated to 10°C were maintained at 10°C (control) or exposed to 15°C for either 24 h (acute exposure) or 50 days (chronic exposure). Fish exposed to 15°C exhibited reduced growth, higher standard, and maximum metabolic rates compared to those at 10°C. In the chronically exposed group at 15°C, metabolic rates were lower than those of acutely exposed fish. The absolute aerobic scope exhibited no significant variation in temperatures; however, the factorial scope showed a notable reduction at 15°C in both acute and chronic exposed groups, aligning with a correlated decrease in individual growth rates. Chronic warming led to increased plasma glucose levels, indicating energy mobilization, but cortisol levels were unaffected. Furthermore, chronic warming resulted in reduced intestinal barrier function, as evidenced by increased ion permeability and a negative potential in the serosa layer. We conclude that warming elevates metabolic rates while reducing intestinal barrier function, thus increasing energy expenditure, collectively, limiting energy available for growth at this temperature from increased allostatic load. Thus, juvenile wolffish maintaining their aerobic scope under thermal stress experience slower growth. This research provides insights for improving the welfare and resilience of wolffish in aquaculture at elevated temperatures and understanding their response to increased environmental temperatures.</p>","PeriodicalId":15794,"journal":{"name":"Journal of fish biology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ivana Soledad Friedman, Edgardo Martín Contreras, Analia Verónica Fernández-Gimenez
{"title":"Recovery of alkaline proteinases from fisheries wastes: biochemical characterization and applications.","authors":"Ivana Soledad Friedman, Edgardo Martín Contreras, Analia Verónica Fernández-Gimenez","doi":"10.1111/jfb.16038","DOIUrl":"https://doi.org/10.1111/jfb.16038","url":null,"abstract":"<p><p>Fish visceral waste, which is normally discarded, is considered one of the richest sources of proteinases with potential biotechnological applications. For this reason, alkaline proteinases from viscera of Argentine hake Merluccius hubbsi, Brazilian flathead Percophis brasiliensis, Brazilian codling Urophycis brasiliensis, and stripped weakfish Cynoscion guatucupa were characterized. Individuals were caught by a commercial fleet off the coast of the Argentinean Sea. The intestine and pyloric caeca were dissected out and then minced and triturated with distilled water. The proteinase activity of P. brasiliensis extracts was enhanced by all the ions tested (Mn<sup>2+</sup>, K<sup>+</sup>, Na<sup>+</sup>, Ca<sup>+2</sup>) while the enzymes of the other species were stable in the presence of those ions, retaining more than 60% of their enzymatic activity. Alkaline proteinases of all species showed extreme stability to 5% v/v surfactants at 60 min (Sodium dodecyl sulfate, Triton X-100, Tween 20, Tween 80), and relative stability toward an 6% v/v oxidizing agent (H<sub>2</sub>O<sub>2</sub>) and organic solvents 80% (acetone, isopropanol, methanol, ethanol). The enzyme extracts were incubated for 60 min with these compounds. Interestingly, alkaline proteinases from all species were compatible with the commercial detergents (Ala, Skip, and Ace). These results demonstrate that proteinases recovered from a no-cost sample such as fishery residues can be used for industrial applications, such as detergent formulations.</p>","PeriodicalId":15794,"journal":{"name":"Journal of fish biology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}