Valeria Imeneo, Rosa Romeo, A. De Bruno, A. Piscopo
{"title":"Green-sustainable extraction techniques for the recovery of antioxidant compounds from “citrus Limon” by-products","authors":"Valeria Imeneo, Rosa Romeo, A. De Bruno, A. Piscopo","doi":"10.1080/03601234.2022.2046993","DOIUrl":"https://doi.org/10.1080/03601234.2022.2046993","url":null,"abstract":"Abstract In this work, optimized techniques of conventional, ultrasound and microwave-assisted extraction were applied for the recovery of antioxidant compounds from lemon by-products (Citrus limon L). Specifically, the effect of solvent, temperature, microwave power, time and their interaction on the extraction was investigated. Among the tested solvents, the hydroalcoholic mixture (ethanol:water, 50:50) was the optimal one for all extraction techniques: in particular assisted by ultrasounds at 70 °C for 30 minutes (total phenolic content: 6.93 mg GAE g−1, total flavonoids: 2.07 mg CE g−1, ABTS assay: 18.36 µM TE g−1). Also, the other techniques allowed to obtain valuable extracts, although with relative lower amounts. The analyses of individual phenols revealed hesperidin and eriocitrin as the main compounds (respectively about 1650 and 1150 mg kg−1) after ultrasound assisted and conventional extraction. Results of this work can be useful to valorize an industrial by-product by sustainable techniques for the high-added value substances recovery.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"20 1","pages":"220 - 232"},"PeriodicalIF":0.0,"publicationDate":"2022-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87433469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Bragante, V. Sinhorin, M. M. Sugui, Ana Paula Simões da Cunha, Weslley Bressan Dos Santos, A. Sinhorin
{"title":"In vivo mutagenic effects and oxidative stress parameters evaluation of cypermethrin and benzoate of emamectin and their mixtures in female mice","authors":"W. Bragante, V. Sinhorin, M. M. Sugui, Ana Paula Simões da Cunha, Weslley Bressan Dos Santos, A. Sinhorin","doi":"10.1080/03601234.2022.2045841","DOIUrl":"https://doi.org/10.1080/03601234.2022.2045841","url":null,"abstract":"Abstract We evaluated the biological effects of ingestion by gavage, for 28 days, of the pesticides cypermethrin (CP) and emamectin benzoate (EB) and their mixtures in female Swiss mice. The groups were Control (water); CP; EB and three distinct concentrations of CP and EB mixture expressed in mg/kg/day. The biological effects were analyzed in the complete blood count and plasma (alkaline phosphatase (ALP), alanine aminotransferase (ALT) and creatinine); the biochemical parameters of oxidative stress (substances reactive to thiobarbituric acid (TBARS); reduced glutathione (GSH); catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferase (GST)), and bone marrow cells obtained from the femur for the micronucleus (MN) test. In the heart, there was a reduction in GSH in the groups (0.5 + 0.67 and 2.5 + 3.37), although in the brain this effect appeared for the other groups, except EB. Brain TBARS increased in CP and in the group (2.5 + 3.37) and platelets increased in the group (12.5 + 16.87). Genotoxic/mutagenic effects, showing a consistent increase dose-dependent effect on micronucleus counting for in the female mice. After 28 days of treatment, we can observe that the pesticide mixtures promoted genotoxic damage and oxidative brain damage in female mice, which can damage the health of these animals and possibly their future offspring.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"47 1","pages":"211 - 219"},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90268569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Vondruška, J. Šíma, M. Kobera, Lukáš Rokos, M. Šeda, L. Svoboda
{"title":"Detrimental and essential elements in fruiting bodies of wild-growing fungi Coprinus comatus, Flammulina velutipes, and Armillaria ostoyae","authors":"J. Vondruška, J. Šíma, M. Kobera, Lukáš Rokos, M. Šeda, L. Svoboda","doi":"10.1080/03601234.2022.2046994","DOIUrl":"https://doi.org/10.1080/03601234.2022.2046994","url":null,"abstract":"Abstract Edible medicinal wild-growing fungi Coprinus comatus, Flammulina velutipes, and Armillaria ostoyae were analyzed with regard to the contents of 21 elements in their fruiting bodies. The samples were collected from selected sites in South Bohemia, the Czech Republic. C. comatus concentrated Ag, Cd, Cu, Se, and Rb with bioconcentration factors of 12, 2.5, 2.3, 1.8, and 1.1, respectively. High contents (all values expressed in mg kg−1 dry matter) of Al (260), Ca (480), Cu (61), Fe (340), Mg (1400), and Zn (86) were determined for this species. F. velutipes was characterized with markedly high contents of Ca (360), Fe (110), Mg (1200), Mn (26), and Zn (98), respectively. A considerably high content of Ag (5.6) was revealed for A. ostoyae. High contents of Ca (150), Cu (28), Fe (190), Mg (1100), Mn (30), and Zn (40) were determined in fruiting bodies of this species as well. The data concerning the detrimental elements in fruiting bodies of studied fungi indicate no considerably negative effect on human health if they are consumed as a delicacy or used in alternative medicine.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"36 1","pages":"243 - 251"},"PeriodicalIF":0.0,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89622795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fernanda Salles Cunha Peres, F. A. Petter, A. Sinhorin, Larissa Borges de Lima, T. R. Tavanti, Onã da Silva Freddi, Ben Hur Marimon Júnior
{"title":"Influence of biochar on the sorption and leaching of thiamethoxan in soil","authors":"Fernanda Salles Cunha Peres, F. A. Petter, A. Sinhorin, Larissa Borges de Lima, T. R. Tavanti, Onã da Silva Freddi, Ben Hur Marimon Júnior","doi":"10.1080/03601234.2022.2050126","DOIUrl":"https://doi.org/10.1080/03601234.2022.2050126","url":null,"abstract":"Abstract This study evaluated the long-term effect of biochar application on the sorption and desorption of thiamethoxam in a Haplic Plinthosol. The experiment was conducted in a randomized block design with combination of doses of mineral fertilizer NPK (0 and 300 kg·ha−1 formula 05-25-15) and biochar (0, 16 and 32 Mg ha−1). Deformed soil samples were collected in the field from the 0-0.10 m layer of all plots to determine the sorption and desorption of the thiamethoxam insecticide in the soil; fulvic acid (FA), humic acid (AH) and humin (HUM) carbon contents; and total organic carbon. The Batch slurry method was used to evaluate sorption and desorption. The Freundlich isotherm adequately described thiamethoxam sorption in all treatments. The application of biochar increased the sorption (Kfs) and decreased the desorption (Kfd) of thiamethoxam. The sorption intensity (1/n) showed reduction characteristics as the soil concentration of thiamethoxam increased. Biochar has a sorption effect on the soil through covalent bonds and H-bonds with the insecticide molecules, thereby indirectly increasing the sorption potential in the chemical fractions of the organic matter of soil. The application of 32 Mg ha−1 of biochar significantly reduced thiamethoxam in the soil solution. Altogether, the present study reveals that biochar application in soil is a promising tool for mitigating the contaminant potential of thiamethoxam in subsurface waters.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"38 1","pages":"153 - 163"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86782125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeffesson de Oliveira-Lima, Rafaela Luiza Dias da Cunha, L. de Brito-Gitirana
{"title":"Effect of benzophenone-3 on the blood cells of zebrafish (Danio rerio)","authors":"Jeffesson de Oliveira-Lima, Rafaela Luiza Dias da Cunha, L. de Brito-Gitirana","doi":"10.1080/03601234.2021.2022946","DOIUrl":"https://doi.org/10.1080/03601234.2021.2022946","url":null,"abstract":"Abstract Benzophenone-3 (BP-3) is a common component of organic sunscreen widely used that can affect especially aquatic ecosystems health, including fish. To verify the biological effects of low concentrations of BP-3 on blood cells, one hundred and forty zebrafish (D. rerio) were used and then randomly divided into five groups: control group (water), solvent group (alcoholic water), and BP-3 group (BP-3 at 7 µg L−1, BP-3 at 70 µg L−1, and BP-3 at 700 µg L−1). The blood slices were stained with Panoptic stain and with Giemsa solution for the hematological analysis. During the exposure to BP-3, no behavioral changes were observed. Although no significant difference in total leukocytes occurred, an increase in neutrophils and a reduction of lymphocytes at the highest concentration on both 7th and 14th days were detected. The total and cytoplasmic area of erythrocytes on the 7th day at the highest concentration were reduced. In addition, alterations on the erythrocyte nuclear morphology in fish exposed to BP-3 were usually visualized, mainly when considered the occurrence of blebbed nucleus and micronucleus, indicating that BP-3 exhibits cytotoxic and mutagenic effects. The results indicate that BP-3 can interfere with the morphophysiology of aquatic organisms.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"26 1","pages":"81 - 89"},"PeriodicalIF":0.0,"publicationDate":"2022-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87351035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marta B Ramalho, A. F. S. Durães, F. Silvério, Gevany P Pinho
{"title":"Determination of three cresol isomers in sewage sludge by solid-liquid extraction with low temperature purification and gas chromatography-mass spectrometry","authors":"Marta B Ramalho, A. F. S. Durães, F. Silvério, Gevany P Pinho","doi":"10.1080/03601234.2019.1678952","DOIUrl":"https://doi.org/10.1080/03601234.2019.1678952","url":null,"abstract":"Abstract Cresols are chemical contaminants derivative from phenol which can be found in sewage sludge. However, little attention has been given to monitoring these compounds in environmental matrices in the literature. Thus, the objective of this study was to develop a simple method based on solid-liquid extraction with low temperature purification for determining three cresol isomers in sludge. The quantification of these compounds was performed by gas chromatography coupled to mass spectrometry with a previous derivatization step. After a detailed study, the cresol recovery was higher than 91%, with relative standard deviation lower than 12% and a limit of quantification of 20 μg kg−1. Linearity was achieved between 10 and 90 μg L−1 (R2 > 0.98) with the standard solutions prepared in matrix extracts due to the trouble caused by the matrix effect. The proposed method was applied with success for monitoring cresols in sewage sludge samples coming from six different wastewater treatment plants. All samples showed contamination by cresols, mainly p-cresol with values between 32.3 and 516.9 μg kg−1. The majority of the analyzed samples showed a total sum of the isomers higher than the maximum residue limit established by Brazilian legislation (160 μg kg−1).","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"30 1","pages":"184 - 192"},"PeriodicalIF":0.0,"publicationDate":"2020-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82096636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. S. Gueretz, Filipe A Da Silva, E. L. Simionatto, J. Férard, C. M. Radetski, C. A. Somensi
{"title":"A multi-parametric study of the interaction between the Parati river and Babitonga Bay in terms of water quality","authors":"J. S. Gueretz, Filipe A Da Silva, E. L. Simionatto, J. Férard, C. M. Radetski, C. A. Somensi","doi":"10.1080/03601234.2019.1685813","DOIUrl":"https://doi.org/10.1080/03601234.2019.1685813","url":null,"abstract":"Abstract The Parati River contributes to the Babitonga Bay water complex, but the contents of the bay also influence the river during periods of inverted currents. In this study, the water quality along four stretches of the Parati River and Babitonga Bay was evaluated using chemical (physico-chemical and chromatographic analysis), microbiological (fluorescein diacetate hydrolysis) and ecotoxicological (Lumistox) methods to assess the reciprocal influence of the waters of this river-bay system. In addition, the most appropriate type of analysis for the monitoring of the estuarine region of the Parati River was identified. The results of six sampling campaigns showed that the type of contaminants and their levels varied temporally and spatially and thus the water quality also changed. Anthropogenic activity, such as banana cultivation and the release of sewage into the water system, is the primary cause of the contamination that affects the quality of the water in the Parati River estuary, which is a crucial ecological niche for the reproduction of various marine species. The ecotoxicity tests with Aliivibrio fischeri conducted to evaluate the water quality showed an integrative response, and the ecotoxicity data indicated that the Parati River does not have a significant influence on the water quality of Babitonga Bay.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"25 1","pages":"257 - 264"},"PeriodicalIF":0.0,"publicationDate":"2020-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89913844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xinyan Guo, Lv Xiaojun, Ai-mei Zhang, Zheng Yan, Siyi Chen, Na Wang
{"title":"Antibiotic contamination in a typical water-rich city in southeast China: a concern for drinking water resource safety","authors":"Xinyan Guo, Lv Xiaojun, Ai-mei Zhang, Zheng Yan, Siyi Chen, Na Wang","doi":"10.1080/03601234.2019.1679563","DOIUrl":"https://doi.org/10.1080/03601234.2019.1679563","url":null,"abstract":"Abstract The occurrence and distribution in the aquatic environment of Nanjing of 49 antibiotics from seven therapeutic classes were investigated using an improved analytical method developed for multiclass target analysis. The results showed that these antibiotics are widely present in the water bodies of this city, with a total concentration of up to 1.958 μg L−1. The most abundant class was tetracyclines, contributing 43.7% to the total antibiotic burden. Lincomycin was detected in all samples, and the detection rate of clindamycin was 90.5%. An exploratory analysis of the data points was performed by unsupervised pattern recognition (hierarchical cluster analysis, HCA) in an attempt to clarify the pollution level in different sampling areas, and robust cluster solutions grouped the data according to their different antibiotic contaminant profiles. The safety of drinking water resources was emphasized, and the rivers, as the main receiving water body for treated and untreated wastewater in this city, were more seriously contaminated than the surrounding lakes and reservoir, not only in concentration but also in detection frequency, in our study as well as in similar research studies. A correlation analysis between the presence of antibiotics and the environmental factors was conducted, and it was found that antibiotic contamination and water quality were closely connected; the better the water quality, the lower the antibiotic contamination. Positive correlations existed between the antibiotics and tested heavy metals, as well as between antibiotics and boron and arsenic. However, whether these correlations resulted from their reaction or a common source was difficult to determine, and the mechanism requires further exploration.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"1 1","pages":"193 - 209"},"PeriodicalIF":0.0,"publicationDate":"2020-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83511042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization on the toxic mechanism of two fluoroquinolones to trypsin by spectroscopic and computational methods","authors":"Yanxiu Guo, Pengfei Qin, Chaoyun Wang, Xingren Pan, Xiaofei Dong, Wansong Zong","doi":"10.1080/03601234.2019.1685317","DOIUrl":"https://doi.org/10.1080/03601234.2019.1685317","url":null,"abstract":"Abstract Ciprofloxacin (CPFX) and enrofloxacin (ENFX), two of the most widely used fluoroquinolones (FQs), pose a great threat to humans and the ecosystem. In this study, the toxic mechanisms between the two FQs and trypsin were evaluated by means of multiple spectroscopic methods, as well as molecular docking. During the fluorescence investigations, both FQs quenched the intrinsic fluorescence of trypsin effectively, which was due to the formation of moderately strong complexes (mainly through van der Waals forces and hydrogen bonds). The binding of two FQs not only caused the conformational and micro-environmental changes of trypsin, but also changed its molecular activity; shown by the UV–Visible absorption spectroscopy, synchronous fluorescence spectroscopy, and functional tests. The established methods in this work can help to comprehensively understand the transport of FQs in the human body.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"34 13 1","pages":"230 - 238"},"PeriodicalIF":0.0,"publicationDate":"2020-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77235525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. V. Buitimea-Cantúa, N. E. Buitimea‐Cantúa, María Del Refugio Rocha-Pizaña, E. Rosas-Burgos, A. Hernández-Morales, J. Molina-Torres
{"title":"Antifungal and anti-aflatoxigenic activity of Heliopsis longipes roots and affinin/spilanthol against Aspergillus parasiticus by downregulating the expression of alfD and aflR genes of the aflatoxins biosynthetic pathway","authors":"G. V. Buitimea-Cantúa, N. E. Buitimea‐Cantúa, María Del Refugio Rocha-Pizaña, E. Rosas-Burgos, A. Hernández-Morales, J. Molina-Torres","doi":"10.1080/03601234.2019.1681818","DOIUrl":"https://doi.org/10.1080/03601234.2019.1681818","url":null,"abstract":"Abstract In the present study, ethanolic extract from Heliopsis longipes roots and affinin/spilanthol against Aspergillus parasiticus growth and aflatoxins production were studied in relation to the expression of aflD and aflR, two key genes of aflatoxins biosynthetic pathway. Phytochemical analysis of the ethanolic extract by GC-EIMS identified affinin/spilanthol (7.84 ± 0.27 mg g−1) as the most abundant compounds in H. longipes roots. The antifungal and anti-aflatoxigenic assays showed that affinin/spilanthol at 300 µg mL−1 produced the higher inhibition of radial growth (95%), as well as, the higher aflatoxins production inhibition (61%) in comparison to H. longipes roots (87% and 48%, respectively). qRT-PCR revealed that the expression of aflD and aflR genes showed a higher downregulation in affinin/spilanthol at 300 µg mL−1. The expression ratio of alfD was suppressed by affinin/spilanthol in 79% and aflR in 84%, while, a lower expression ratio suppressed by H. longipes was obtained, alfD (55%) and aflR (59%). Affinin/spilanthol possesses higher antifungal and anti-aflatoxigenic activity against A. parasiticus rather than H. longipes roots, and this anti-aflaxotigenic activity occurring via downregulation of the aflD and aflR genes. Thus, H. longipes roots and affinin/spilanthol can be considered potent antifungal agents against aflatoxigenic fungus, especially, affinin/spilanthol.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"8 1","pages":"210 - 219"},"PeriodicalIF":0.0,"publicationDate":"2020-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83659623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}