Journal of Digestive Diseases最新文献

{"title":"Multidimensional Optimization of Small Bowel Capsule Endoscopy With Transcutaneous Electrical Acustimulation: A Randomized Controlled Trial","authors":"Yi Long Liu,&nbsp;Bei Fang Ning,&nbsp;Ya Nan Mao,&nbsp;Yu Hao Song,&nbsp;Kai Ming Wu,&nbsp;Xia Sheng,&nbsp;Kai Ding,&nbsp;Jiande D. Z. Chen,&nbsp;Wei Tan,&nbsp;Wei Fen Xie","doi":"10.1111/1751-2980.70053","DOIUrl":"10.1111/1751-2980.70053","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To evaluate whether transcutaneous electrical acustimulation (TEA) during bowel preparation for small bowel capsule endoscopy (SBCE) can reduce the incidence of gastrointestinal adverse events (AEs), shorten gastric transit time (GTT), improve small bowel visualization quality, and enhance patient acceptability.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We prospectively enrolled outpatients scheduled for SBCE during December 2023 and March 2025. The participants were randomly allocated to receive either TEA at bilateral ST36 acupoints or sham stimulation (control) at non-acupoints during bowel preparation with split-dose polyethylene glycol. The primary outcome was the overall incidence of gastrointestinal AEs. Secondary outcomes included AE severity, small bowel visualization quality, GTT, and lesion detection rate, among others.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 39 participants completed the trial. Compared with the control group, TEA significantly reduced the overall incidence of gastrointestinal AEs during bowel preparation (<i>p</i> = 0.004), with particularly notable reductions in nausea and abdominal bloating. The overall severity of AEs was also markedly lower in the TEA group (<i>p</i> &lt; 0.001). Furthermore, TEA significantly improved visualization quality scores for both the entire (<i>p</i> = 0.003) and distal small bowel (<i>p</i> = 0.030). GTT was significantly shorter in the TEA group (<i>p</i> &lt; 0.001), and patient-reported scores for comfort (<i>p</i> = 0.027) and acceptability (<i>p</i> = 0.015) were significantly higher in the TEA group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>TEA during SBCE bowel preparation significantly reduces gastrointestinal AEs, enhances small bowel visualization quality, and accelerates GTT. TEA is a safe and well-tolerated intervention, making it a promising adjunct to optimize SBCE.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15564,"journal":{"name":"Journal of Digestive Diseases","volume":"27 5-6","pages":"214-224"},"PeriodicalIF":2.7,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spasmolytic Polypeptide-Expressing Metaplasia in Chronic Atrophic Gastritis and Its Role in the Progression From Gastritis to Gastric Cancer","authors":"Xin Quan,&nbsp;Ling Long Ji,&nbsp;Tian He,&nbsp;Jiao Liu,&nbsp;Lei Shi,&nbsp;Ling Liu","doi":"10.1111/1751-2980.70060","DOIUrl":"10.1111/1751-2980.70060","url":null,"abstract":"<div>\u0000 \u0000 <p>Chronic atrophic gastritis (CAG) is a well-recognized precursor of gastric cancer (GC). Spasmolytic polypeptide-expressing metaplasia (SPEM) is a key intermediate state during this process, representing a reparative response to gastric mucosal injury, and may promote the development of malignancy under persistent inflammation. Recent studies have suggested that SPEM is not a static lesion but a dynamic and heterogeneous process regulated by epithelial injury, oxyntic atrophy, and chronic inflammation. This review summarizes current knowledge on cellular plasticity, lineage dynamics, and molecular mechanisms underlying SPEM formation and progression in CAG. We focus on recent advances in oxyntic atrophy, immune cell and inflammation, as well as oxidative stress in gastric epithelial remodeling, and highlight emerging evidence implicating ferroptosis, a regulated form of iron-dependent lipid peroxidation, in gastric mucosal inflammation and SPEM development. By integrating classical models of GC with recent mechanistic insights, we aim to discuss the role of SPEM in CAG-associated GC risk and to identify potential therapeutic targets related to oxyntic atrophy, inflammation, and ferroptosis. These emerging findings offer new perspectives for future research aiming at risk stratification and precision prevention.</p>\u0000 </div>","PeriodicalId":15564,"journal":{"name":"Journal of Digestive Diseases","volume":"27 5-6","pages":"190-204"},"PeriodicalIF":2.7,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148430252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality of Helicobacter pylori Infection Management Through Telemedicine: A Nationwide Cross-Sectional Study","authors":"Miao Duan,&nbsp;Kun Ping Ju,&nbsp;Xiao Yun Yang,&nbsp;Yan Qing Li,&nbsp;Yue Yue Li,&nbsp;Qing Zhou Kong,&nbsp;Xiu Li Zuo","doi":"10.1111/1751-2980.70061","DOIUrl":"10.1111/1751-2980.70061","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To evaluate the clinical practice and quality of first-line <i>Helicobacter pylori</i> eradication therapy by gastroenterologists in direct-to-consumer telemedicine (DTCT) settings.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The study was conducted between November 2024 and February 2025 using unannounced standardized patients (USPs). Six trained and validated USPs questioned clinicians using specific <i>H. pylori</i> infection scenarios on hospital- or enterprise-sponsored DTCT platforms. Accessibility and effective consultation rates of the enquiry platforms were evaluated. The consultation process at each visit was also analyzed for guideline adherence and patient-centeredness.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>USPs conducted 552 visits on 53 hospital- and 19 enterprise-sponsored accessible platforms, with effective consultation rate of 65.6% (362 of 552). Based on a 10-point quality checklist, the overall guideline adherence score was 5.0, which was significantly higher for the enterprise-sponsored platforms than for hospital platforms (5.4 ± 2.7 vs. 4.8 ± 2.8, <i>p</i> = 0.045). When stratified by professional title of the clinicians involved, clinicians with intermediate title achieved a higher guideline adherence score than those with junior or senior titles (5.5 ± 2.7 vs. 4.3 ± 2.7 vs. 4.8 ± 2.8, <i>p</i> = 0.009). Altogether 72% of clinicians prescribed the correct treatment regimen for <i>H. pylori</i> infection. Furthermore, patient satisfaction during the treatment process was generally acceptable.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We identified gaps in guideline adherence and clinical standardization for telemedicine-based <i>H. pylori</i> eradication in China. Further nationwide standardized training for gastroenterologists should prioritize structured history-taking, appropriate prescribing, resistance-guided antibiotic selection, and patient follow-up management.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Trial Registration</h3>\u0000 \u0000 <p>ClinicalTrials.gov ID: NCT06749873</p>\u0000 </section>\u0000 </div>","PeriodicalId":15564,"journal":{"name":"Journal of Digestive Diseases","volume":"27 5-6","pages":"205-213"},"PeriodicalIF":2.7,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148471068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Submucosal Pressure Ejection Endoscopic Dissection (SPEED): A New Technique in Increasing the Safety and Efficacy of ESD (With Video)","authors":"Tao Chen,&nbsp;Zhu Yun Leng,&nbsp;Ai Ping Xu,&nbsp;Jing Jing Lian,&nbsp;Hai Bin Zhang,&nbsp;Li Shen,&nbsp;Li Zhang,&nbsp;Mei Dong Xu","doi":"10.1111/1751-2980.70064","DOIUrl":"10.1111/1751-2980.70064","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The universal adoption of endoscopic submucosal dissection (ESD) is limited by its technical demands, slow learning curve, long procedure time, and high risks of bleeding, muscular layer injury, and perforation. We developed the submucosal pressure ejection endoscopic dissection (SPEED) technique that combines pressure injection, incision, dissection, and coagulation and avoids frequent device exchange during ESD. We aimed to introduce this novel SPEED strategy and evaluate its therapeutic advantages for superficial gastrointestinal neoplasms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Altogether 985 patients who underwent ESD for early gastrointestinal tumors between January 2020 and April 2024 were included and divided into the SPEED group and the conventional ESD group. Clinical outcomes, including en bloc and R0 resection rates, resection time, intraprocedural application of hemostatic forceps, procedure-related adverse events, and local recurrence, were evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>SPEED achieved en bloc and R0 resection rates of 99.85% and 99.40%, respectively. Compared with conventional ESD, SPEED was associated with a shorter median resection time (33 min vs. 57 min), less frequent intraprocedural application of hemostatic forceps (2.71% vs. 19.25%), a lower intraprocedural perforation rate (0.15% vs. 2.48%), and a lower post-procedure hemorrhage rate (1.51% vs. 5.90%).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>With the additional high-pressure injection, the SPEED technique greatly improves ESD efficiency and ensures a clearer visual field during ESD, thereby reducing risk of complications. It efficiently improves the speed and safety of dissection during ESD.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15564,"journal":{"name":"Journal of Digestive Diseases","volume":"27 5-6","pages":"225-233"},"PeriodicalIF":2.7,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-2980.70064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148706530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes of Different Guidewire-Assisted Techniques for Recurrent Malignant Biliary Obstruction After Uncovered Metallic Stent Placement","authors":"Qiao Feng Chen,&nbsp;Zi Hang Xu,&nbsp;You Hua Wang,&nbsp;Zhen Zhen Yang,&nbsp;Shi Yu Zhang,&nbsp;Ya Wei Xing,&nbsp;Guo Hua Li,&nbsp;You Xiang Chen,&nbsp;Xiao Jiang Zhou,&nbsp;Jun Bo Hong","doi":"10.1111/1751-2980.70065","DOIUrl":"10.1111/1751-2980.70065","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>We aimed to compare the clinical outcomes of the bent guidewire insertion (BGI) technique and the conventional straight guidewire insertion (SGI) technique for managing recurrent malignant biliary obstruction (RMBO) after initial uncovered metallic stent (UMS) placement.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Ninety patients who underwent endoscopic intervention for RMBO after UMS placement were retrospectively included. Clinical outcomes and endoscopic retrograde cholangiopancreatography (ERCP)-related factors were compared between the SGI and BGI groups. Univariate and multivariate logistic regression analyses were performed to identify the independent risk factors associated with difficult cannulation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>There were 43 and 47 patients who underwent the SGI and BGI techniques, respectively, for intervention of RMBO. The SGI group had significantly higher rates of difficult cannulation (48.8% vs. 19.1%, <i>p</i> = 0.006) and guidewire insertion technique transformation (44.2% vs. 4.3%, <i>p</i> &lt; 0.001). Additionally, procedure time (49.7 min ± 23.4 min vs. 34.7 min ± 20.5 min, <i>p</i> = 0.002) and cannulation time (5.0 min vs. 4.0 min, <i>p</i> = 0.005) were longer in the SGI group. Technical and clinical success rates, procedure-related complications, and length of hospitalization were comparable between the two groups. Multivariate analysis revealed that SGI (odds ratio [OR] 4.682, 95% confidence interval [CI] 1.643–13.340, <i>p</i> = 0.004) and hilar biliary tract lesions (OR 3.673, 95% CI 1.236–10.915, <i>p</i> = 0.019) were identified as independent factors for difficult biliary cannulation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>BGI technique may be superior to SGI approach for re-intervention in patients with RMBO after initial UMS placement.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15564,"journal":{"name":"Journal of Digestive Diseases","volume":"27 5-6","pages":"243-251"},"PeriodicalIF":2.7,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “AMPK Activation Alleviated Dextran Sulfate Sodium-Induced Colitis by Inhibiting Ferroptosis”","authors":"","doi":"10.1111/1751-2980.70062","DOIUrl":"10.1111/1751-2980.70062","url":null,"abstract":"<p>\u0000 <span>S. P. Sun</span>, <span>Y. F. Lu</span>, <span>H. Li</span>, et al., “ <span>AMPK activation alleviated dextran sulfate sodium-induced colitis by inhibiting ferroptosis</span>”, <i>Journal of Digestive Diseases</i> <span>24</span>, no. <span>3</span> (<span>2023</span>): <span>213</span>–<span>223</span>.\u0000 </p><p>In pAMPK-stained histopathological image of Figure 3C, we identified an unintentional image-layout error for the ulcerative colitis (UC) group. Figure 3 in the initial submission was correct. While at the revision stage, to standardize the scale bars and font sizes across the figures, we re-exported and reassembled the source file. During this process, the phosphorylated adenosine monophosphate-activated protein kinase (p-AMPK)-stained and GPX4-stained images for the UC group in Figure 3 appeared identical in the final version.</p><p>This error is limited solely to image arrangement and assembly mistake. It does not affect the authenticity of the original data, the statistical significance of the results, or the final conclusions of the article.</p><p>The correct Figure 3 is displayed below:</p><p>We apologize for this error.</p>","PeriodicalId":15564,"journal":{"name":"Journal of Digestive Diseases","volume":"27 5-6","pages":"266-267"},"PeriodicalIF":2.7,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1751-2980.70062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148678934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Impact of Endoscopic Ultrasound-Guided Fine-Needle Biopsy in Radiologically Suspected Pancreatic Neuroendocrine Tumors.","authors":"Carolina González-Abós, Ignacio Gil, Àngels Ginés, Guillem Soy, Oriol Sendino, Iván Archilla, Fabio Ausania","doi":"10.1111/1751-2980.70069","DOIUrl":"https://doi.org/10.1111/1751-2980.70069","url":null,"abstract":"<p><strong>Objectives: </strong>Incidental diagnosis of pancreatic neuroendocrine tumors (PNETs) is increasing. Its surgical indication depends on the risk of progression, whereas histological features can be assessed by endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB). However, its systematic use remains a matter of debate. We aimed to analyze the impact of EUS-FNB on therapeutic management in patients with radiologically suspected PNETs.</p><p><strong>Methods: </strong>In this retrospective observational single-center study, all patients with suspected PNET by radiological imaging who underwent EUS-FNB from 2011 to 2023 were enrolled. Patients with multiple endocrine neoplasia or previous/metastatic tumors were excluded. The North American Neuroendocrine Tumor Society (NANETS) consensus was followed, and modifications in management due to FNB findings were detailed.</p><p><strong>Results: </strong>Of 212 patients undergoing EUS-FNB for suspected PNETs, 146 patients were included. No severe adverse events were reported during the biopsy procedure. Surgical pathology showed that the median tumor size was 17 mm, 32.2% were located in the pancreatic head. Therapeutic management was modified in 12 (8.2%) patients based on EUS-FNB findings due to different tumor types or additional high-risk tumor feature (n = 5), different tumor grading (n = 4), and absence of malignancy (n = 3).</p><p><strong>Conclusion: </strong>Systematic EUS-FNB improves accuracy in PNET diagnosis and is associated with a non-negligible rate of change in management. Large-scale studies are needed to confirm our findings.</p>","PeriodicalId":15564,"journal":{"name":"Journal of Digestive Diseases","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148879919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Proton-Pump Inhibitors and Muscle Strength in Older Adults: A Population-Based Analysis.","authors":"Jaakko Ollila, Konsta Teppo, Kimmo Salminen, Adriana Lääti, Jenni Vire, Matti Viitanen, Ville Langén","doi":"10.1111/1751-2980.70068","DOIUrl":"https://doi.org/10.1111/1751-2980.70068","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to assess the association of proton-pump inhibitor (PPI) use with muscle strength in older adults.</p><p><strong>Methods: </strong>All 1940-born residents of Turku, Finland were surveyed during 2016 and 2017 as part of the New Turku Older Adults Study. Associations between PPI use, regardless of dosing pattern, and physical function were analyzed using Welch's t-test, chi-square test, and linear and logistic regression models. Models were initially run as crude, then adjusted for age, sex, and body mass index (Multivariable Model 1), and further adjusted for the Charlson Comorbidity Index (CCI) (Multivariable Model 2).</p><p><strong>Results: </strong>Among 616 participants (63.1% females), PPI users had lower mean handgrip strength (27.6 kg vs. 30.1 kg, p = 0.019), slower median 4-m walking performance (3.7 s vs. 3.5 s, p = 0.017), and lower success in the armchair push-pull test (86.9% vs. 95.4%, p = 0.002) compared with non-users. Crude regression analyses showed poorer performance across all outcomes among PPI users (all p < 0.05); however, this persisted only for the armchair push-pull test through Multivariable Model 2 (odds ratio 0.40, 95% confidence interval 0.19-0.85, p = 0.02). In the sensitivity analysis, replacing the CCI with non-vitamin medication burden attenuated this association as well (p > 0.05).</p><p><strong>Conclusions: </strong>PPI use in older adults is significantly associated with lower muscle strength and poorer physical performance in less adjusted models, with these associations attenuating and disappearing with stepwise adjustment. These findings underscore the need to consider residual confounding in studies linking PPI use to adverse outcomes.</p>","PeriodicalId":15564,"journal":{"name":"Journal of Digestive Diseases","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148874310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retention of a Patency Capsule Shell in a Nonsteroidal Anti-Inflammatory Drug-Induced Small-Bowel Stricture, Detected and Retrieved by Balloon-Assisted Enteroscopy: Emphasizing the Importance of Confirming Capsule Excretion.","authors":"Shinichiro Kawatoko, Tomohiro Nagasue, Akihiro Shigemori, Akihiro Yoshida, Junji Umeno","doi":"10.1111/1751-2980.70058","DOIUrl":"https://doi.org/10.1111/1751-2980.70058","url":null,"abstract":"","PeriodicalId":15564,"journal":{"name":"Journal of Digestive Diseases","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148317359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Neutrophil Extracellular Trap-Related Proteins Serving as Potential Diagnostic and Disease Activity Biomarkers of Cronkhite–Canada Syndrome","authors":"Run Feng Zhang,&nbsp;Yun Fei Zhi,&nbsp;Cheng Zhu Ou,&nbsp;Wen You,&nbsp;Shuang Liu,&nbsp;Qiu Shi Xu,&nbsp;Tian Ming Xu,&nbsp;Qi Pu Wang,&nbsp;Hao Tang,&nbsp;Jing Nan Li,&nbsp;Ji Li","doi":"10.1111/1751-2980.70046","DOIUrl":"10.1111/1751-2980.70046","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To explore the involvement of neutrophil dysregulation in Cronkhite–Canada syndrome (CCS) and to identify serum biomarkers for its diagnosis and disease activity assessment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We performed comprehensive serum proteomic analysis using data-independent acquisition (DIA) on samples from patients with active CCS (aCCS) and healthy controls (HCs). Candidate proteins were further evaluated by parallel reaction monitoring (PRM). An independent validation cohort including cases with aCCS, remitting CCS (rCCS), and HCs was then assessed using enzyme-linked immunosorbent assay (ELISA). In addition, previously generated transcriptomic data and immunofluorescence staining of colonic polyps were used to assess local neutrophil extracellular trap (NET)-related immune changes in intestinal tissues.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Proteomic screening identified 362 differentially expressed proteins, and bioinformatic analysis consistently highlighted pathways related to neutrophil activation, acute inflammation, and NET formation. Key neutrophil-associated proteins, including myeloperoxidase (MPO), lipocalin-2 (LCN2), and matrix metalloproteinase-9 (MMP-9), were significantly upregulated. PRM validated seven upregulated candidate proteins. In the independent validation cohort, compared with HCs, serum MPO-DNA complexes, LCN2, and MMP-9 were significantly elevated in aCCS patients, whereas MPO-DNA complexes and MMP-9 were significantly reduced in rCCS cases. Tissue-level transcriptomic and immunofluorescence analyses further supported NET-related immune activation in CCS colonic polyps. Receiver operating characteristic curve analysis demonstrated favorable diagnostic performance for these biomarkers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our findings support the involvement of neutrophil dysregulation and NET-associated pathways in CCS pathobiology. Serum MPO-DNA complexes, LCN2, and MMP-9 may serve as promising non-invasive biomarkers for diagnosis and disease activity monitoring. Further studies incorporating disease control cohorts and mechanistic validation are warranted.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15564,"journal":{"name":"Journal of Digestive Diseases","volume":"27 3-4","pages":"129-138"},"PeriodicalIF":2.7,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147772838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}