JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH最新文献

{"title":"Effect of Mind Sound Resonance Technique on Selected Psycho-emotional Well-being Parameters in Secondary School Students: A Randomised Controlled Trial","authors":"","doi":"10.7860/jcdr/2023/61132.17626","DOIUrl":"https://doi.org/10.7860/jcdr/2023/61132.17626","url":null,"abstract":"Introduction: Mind Sound Resonance Technique (MSRT) is a yogic relaxation technique, which creates a resonance of the “A,” “U,” “M,” “AUM,” and Mahämåtyuïjaya mantra chanting to provide a delicate massage sensation throughout the body. Adolescence is a developmental stage marked by changes in social, emotional, and cognitive functioning. Studies have demonstrated the benefits of yoga, pranayama, and meditation regarding health and mental issues in both adolescents and adults. Aim: To see the effect of the MSRT on selected psycho-emotional parameters in secondary school students. Materials and Methods: In this randomised controlled trial, data of total 63 adolescents (intervention group (n=32) and control group (n=31) were analysed, who were selected from two English medium schools in Udaipur, Tripura (northeast India). The intervention group was provided the MSRT for 30 minutes each day for 21 sessions, whereas the control group went through only the daily routine, in the month of April 2022. The data were collected using questionnaires, prior to and after the intervention. Independent sample test and paired sample t-test were done for ‘between groups’ and ‘within group’ comparison respectively for the variables i.e., emotion regulation, impulsivity, creative thinking, critical thinking, and interpersonal relationship. Mann-whitney test and Wilcoxon-signed rank test were done for ‘between groups’ and ‘within group’ comparison, respectively for the variables i.e., aggression and mindfulness. Results: The intervention group showed significant improvement in mindfulness (p<0.001), creative thinking (p<0.001), critical thinking (p<0.001), interpersonal relationship (p<0.001), emotion regulation (p=0.005) and a significant reduction in aggression (p<0.001) and impulsivity (p=0.001) compared to control group. Conclusion: Based on the result of the present study it is suggested that MSRT can help to improve the psycho-emotional well-being of adolescents.","PeriodicalId":15483,"journal":{"name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","volume":"18 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90947948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Migraine Patterns and White Matter Hyperintensities in MRI Brain: A Cross-sectional Analytical Study","authors":"Jibin Antony, Athul Damodaran Namboothiri, Lenythomas Mathew","doi":"10.7860/jcdr/2023/62607.18539","DOIUrl":"https://doi.org/10.7860/jcdr/2023/62607.18539","url":null,"abstract":"Introduction: Migraine is a neurological condition characterised by recurrent episodes of headaches. The role of Magnetic Resonance Imaging (MRI) as a diagnostic tool in evaluating migraines is yet to be fully understood. Changes that occur in the blood vessels during a migraine attack lead to hypoperfusion in the brain, causing neurovascular dysfunction and cortical spreading depression. These events are manifested as White Matter Hyperintensities (WMHs) in brain MRI. Aim: To determine the association between migraine patterns and WMHs in the MRI brain scans. Materials and Methods: A cross-sectional analytical study was conducted at Department of Neurology, Mar Baselios Medical Mission Hospital, a tertiary care centre for Neurology and Movement disorders in South India, from June 2021 to June 2022. The study included 100 patients aged between 18 and 50 years who had been diagnosed with migraine. A questionnaire was prepared, which included various parameters such as the presence or absence of aura, duration, frequency, tolerability, disability, intensity of pain, nausea, and resistance to treatment. The severity of migraine in the patients was evaluated using the Migraine Intensity and Severity Evaluation (MIGSEV) scale, which considers parameters such as pain intensity, attack duration, nausea, disability, tolerability, treatment resistance, and attack frequency. Based on this assessment, patients were classified into Grade I, II, or III. After obtaining informed consent, MRI brain scans were performed on all patients. Patients exhibiting WMHs underwent Gadolinium IV contrast, and those with contrast-enhancing lesions underwent whole spine screening and CSF analysis testing. Statistical Package for the Social Sciences (SPSS) version 20.0 was used for data analysis. The statistical tests employed included the MannWhitney test, Kruskal-Wallis test and Spearman coefficient. Results: The mean age of the participants was 28.60±7.0 years. WMHs were present in 47 out of 100 patients (47%). A significant association was observed between WMHs and aura (U=487.50) (p-value<0.001). Multivariate analysis revealed that migraine severity grade, pain intensity during the attack, nausea, disability, tolerability, migraine duration, and resistance to treatment were statistically significant with a p-value of <0.001. Conclusion: WMHs in MRI were more significant in patients with Grade III MIGSEV scores. There was a statistically significant association between the number of WMHs, high intensity of pain, vomiting, severe disability, and tolerability during a migraine attack. Therefore, these parameters can be considered as risk factors for developing WMHs in the brain MRI of migraine patients.","PeriodicalId":15483,"journal":{"name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","volume":"176 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135101989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Retention Rates of Pit and Fissure Sealants Placed on First Permanent Molars Treated with Air Abrasion and Acid Etching: A Split-mouth Randomised Clinical Trial","authors":"Bodike Deepika, C Vinay, KS Uloopi, Kakarla Sri Roja Ramya, Penmatsa Chaitanya, MV Ramesh","doi":"10.7860/jcdr/2023/62942.18551","DOIUrl":"https://doi.org/10.7860/jcdr/2023/62942.18551","url":null,"abstract":"Introduction: Pit and fissure sealants are a reliable and safe method of occlusal caries prevention. Accurate preconditioning of the enamel surface is essential for long-term sealant retention. Aim: To assess the retention rate of pit and fissure sealants applied to first permanent molars treated with air abrasion and acid etching in children aged 6 to 9 years. Materials and Methods: This split-mouth randomised study was conducted in the Department of Paediatric Dentistry, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India from March 2019 to March 2020. The study included 45 children aged 6-9 years with 180 completely erupted maxillary and mandibular first permanent molars. The children were randomly divided into two groups: Group-I (Air Abrasion) and Group-II (Conventional Acid Etch). After pretreatment of the enamel surface with either air abrasion or acid etching, sealant was applied. Sealant retention was evaluated at 6- and 12-month intervals using Tonn and Ryge’s scoring criteria under a dental operating microscope. Statistical analysis was performed using the Wilcoxon Signedrank Test and the Mann-Whitney U Test. Results: The mean age of the study participants was 7.6±0.5 years at the 12-month interval, complete sealant retention was observed in 23% of teeth in the air abrasion group and 21% in the acid etch group. The p-value of 0.657 indicates that the difference was not statistically significant. The comparison of sealant retention rates in maxillary and mandibular molars treated with both air abrasion (p=0.191, p=0.881) and acid etching (p=1.000, p=0.574) showed no statistically significant differences at both the 6- and 12-month intervals. Conclusion: The retention rate of fissure sealants on enamel surfaces treated with air abrasion was comparable to acid etching. There was no difference in sealant retention rates between maxillary and mandibular molars in both the air abrasion and acid etch groups.","PeriodicalId":15483,"journal":{"name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135106058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel STXBP2 Mutation Causing Familial Haemophagocytic Lymphohistiocytosis Type 5 in a Preterm Neonate with Fatal Outcome: A Case Report","authors":"Laxman Basany, Vinay Batthula, Priyanka Naga Gandrakota, Navya Mamidi, Upparpally Pooja Reddy","doi":"10.7860/jcdr/2023/65845.18559","DOIUrl":"https://doi.org/10.7860/jcdr/2023/65845.18559","url":null,"abstract":"Familial Haemophagocytic Lymphohistiocytosis (FHL) is an autosomal recessive disorder characterised by a hyperinflammatory state due to widespread infiltration of organs with macrophages and lymphocytes. Haemophagocytic Lymphohistiocytosis (HLH) presents with fever, hepatosplenomegaly, cytopenia, hyperferritinemia and haemophagocytosis in the reticuloendothelial tissues causing multi organ failure with fatal outcome. HLH is rare in neonates with an incidence of 1 in 50,000 to 1 in 150,000. FHL is diagnosed based on clinical criteria, biochemical abnormalities, and genetic mutation. Mutations involving the gene STXBP2 contributes to around 10% of cases of FHL and there are only a few cases of FHL5 reported from India. A six-week-old neonate presented with sepsis which was unresponsive to antibiotics. Persistent fever, bicytopenia, hepatosplenomegaly and laboratory tests made us suspect HLH, and evaluate further with whole exome sequencing. FHL5 was diagnosed based on the identification of homozygous missense mutation in exon 3 of STXBP2 gene (chr19: 7642803_7642803delA). The baby succumbed to sepsis and multi organ failure. HLH should be considered in the differential diagnosis of any sick infant who presents with prolonged fever, sepsis unresponsive to antibiotics and an unusual clinical course.","PeriodicalId":15483,"journal":{"name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135211184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Steamed Whole Potato as an Oesophageal Foreign Body","authors":"S Ravi, T Chandramohan, S Parthasarathy","doi":"10.7860/jcdr/2023/66437.18560","DOIUrl":"https://doi.org/10.7860/jcdr/2023/66437.18560","url":null,"abstract":"","PeriodicalId":15483,"journal":{"name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","volume":"4303 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135211512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Intracanal Smear Layer Removal after Passive Microvolume Irrigation with Sodium Hypochlorite and Rotary NickelTitanium Instruments: An In-vitro Study","authors":"MS Priyadarshini, K Madhu Varma, R Kalyan Satish, Indukuri Sai Lakshmi Durga, Dalavai Praveen, M Sowmya","doi":"10.7860/jcdr/2023/64658.18582","DOIUrl":"https://doi.org/10.7860/jcdr/2023/64658.18582","url":null,"abstract":"Introduction: Passive Microvolume Irrigation (PMI) with Sodium Hypochlorite (NaOCl) has been proposed as a new method for root canal disinfection. It is based on the concept that irregular surface characteristics of endodontic instruments can carry NaOCl into the canal space with controlled fluid volume, without applying any pressure. Aim: To compare and evaluate the efficacy of 3% and 5% NaOCl PMI, Saline PMI, and 3% NaOCl Standard Irrigation (SI) in removing the Smear Layer (SL) from the root canal walls at the cervical, middle, and apical thirds of single-rooted teeth. Materials and Methods: This study was conducted in the Department of Conservative Dentistry and Endodontics at Vishnu Dental College, Andhra Pradesh, India. The study was conducted in February 2018. Forty maxillary central incisors with a single canal, extracted within three months of the study, were collected. The teeth were divided into four groups (n=10) based on the irrigation method: Group-1 (3.0% NaOCl SI), Group-2 (3.0% NaOCl PMI), Group-3 (5.0% NaOCl PMI), and Group-4 (Saline PMI). All samples were instrumented upto F4 size with Protaper rotary files, and a final rinse of 17% Ethylenediaminetetraacetic Acid (EDTA) was used. SL removal was evaluated using Scanning Electron Microscope (SEM). The data were analysed using Statistical Package for Social Sciences (SPSS) 21.0. Statistical tests used were Kruskal Wallis and Mann-Whitney-U tests. A p-value of <0.05 was considered statistically significant for all comparisons. Results: There was no significant difference (p>0.05) in SL removal between 3.0% NaOCl SI, 3.0% NaOCl PMI, and 5.0% NaOCl PMI in the middle and apical thirds of the root canals. However, 5.0% NaOCl PMI showed significantly higher efficiency in SL removal at the cervical third compared to the other groups (p<0.05). Conclusion: Passive microvolume irrigation with 3% and 5% NaOCl showed comparable efficacy to 3% hypochlorite SI in the middle and apical thirds of the root canal.","PeriodicalId":15483,"journal":{"name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135261508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal and Foetal Blood Analysis in Term Pregnancies with and without Gestational Diabetes Mellitus: A Prospective Cohort Study","authors":"Krishnaveni Bandi, Mamatha Shivanagappa, C Chaithra","doi":"10.7860/jcdr/2023/63776.18600","DOIUrl":"https://doi.org/10.7860/jcdr/2023/63776.18600","url":null,"abstract":"Introduction: Gestational Diabetes Mellitus (GDM) is the development of carbohydrate intolerance of variable severity with onset or first recognition during pregnancy. Iron is essential for the beta cell functioning of the pancreas and glucose homeostasis in adequate quantities. However, excess iron levels can lead to the generation of an increased amount of free radicals, which can cause toxicity to the pancreatic beta cells, leading to insulin resistance by impairing glucose metabolism. Aim: To compare maternal and foetal blood analysis in term pregnancies with and without GDM. Materials and Methods: This prospective cohort study was conducted at the Department of Obstetrics and Gynaecology, JSS Medical College and Hospital Research Centre, Mysuru, Karnataka, India, on 120 term pregnant women, with 60 cases of GDM and 60 non GDM controls. Maternal blood and cord blood samples were used to measure Haemoglobin (Hb), Packed Cell Volume (PCV), serum iron, and serum ferritin in the mother and newborn at the time of delivery. Foetal blood analysis was performed in terms of foetal haemoglobin, iron, and ferritin. Birth weight was also measured. Statistical analysis was performed using the Chi-square test and Independent t-test, with a p-value <0.05 considered significant. Results: The serum ferritin level of the mother was higher in GDM cases (mean value 89.47 ng/mL) than in non GDM controls (mean value 47.62 ng/mL), and this difference was statistically significant. Serum ferritin levels in newborns were significantly lower in the GDM group (85.43) compared to the non GDM group (102.71). Mean values of haemoglobin, PCV, and iron levels were not significantly higher in newborns of GDM mothers compared to non GDM mothers. Conclusion: In GDM, serum ferritin was increased, indicating a marker of inflammation or iron overload, which increases oxidative stress that might affect placental iron transfer and haemoglobin synthesis in the foetus.","PeriodicalId":15483,"journal":{"name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","volume":"2015 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135263529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser Application in Remineralisation of Enamel after Interproximal Reduction: An In-vitro Scanning Electron Microscopic Study","authors":"Preethi Rajamanickam, Ashwin Mathew George","doi":"10.7860/jcdr/2023/64006.18354","DOIUrl":"https://doi.org/10.7860/jcdr/2023/64006.18354","url":null,"abstract":"Introduction: Interproximal Reduction (IPR) or reproximation of enamel is of prime importance in orthodontics for correcting arch length-tooth size discrepancies. Despite its widespread application, IPR has been associated with adverse effects on the enamel surface. Aim: The aim of this study was to investigate whether laser application, when combined with fluoride application, enhances the remineralisation potential of enamel. Materials and Methods: This in-vitro study was conducted at Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India, from December 2021 to July 2022. It involved 54 extracted teeth, divided into three groups of 18 samples each. Reproximation of 0.25 mm was performed on the proximal enamel surface of each tooth. Group 1 served as the control, group 2 was subjected to Fluor Protector, and group 3 received low-level laser therapy (Er,Cr:YSGG) after Fluor Protector application. Fluor Protector (Ivoclar Vivadent) was applied for seven days. Following the seven-day fluoride administration, a laser treatment using 0.75 W of power and 8.5 J/cm2 of energy was applied for 20 seconds. Microhardness testing was conducted on the samples using a Vickers Hardness Tester. All specimens underwent surface topographic analysis with Scanning Electron Microscopy (SEM) and were evaluated for mineral content (% weight) using SEM-Energy Dispersive X-Ray analysis (EDX). Paired t-tests were performed to compare the pre- and post-microhardness values, while one-way Analysis Of Variance (ANOVA) test and Tukey’s Post-hoc test were used to compare the microhardness values between the groups. Results: The mean microhardness values recorded for group 1, group 2, and group 3 were 209.4±18.4 N/mm2 , 215.16±21.0 N/ mm2 , and 233±18.05 N/mm2 , respectively. ANOVA test revealed a significant difference in microhardness values between group 1 and group 2 (p-value=0.004), as well as between group 1 and group 3 (p-value=0.001). The microhardness value was highest for group 3, followed by group 2 post-intervention. SEM analysis showed that laser-treated enamel surfaces were smoother, with well-coalesced enamel rods. The porous structure of enamel was lost due to fluoride deposition in group 2 and group 3, resulting in a smooth surface. Conclusion: The combined application of fluoride and laser therapy demonstrated synergistic effects in remineralising the slenderised enamel. This simple, non-invasive technique may benefit patients undergoing IPR procedures by reducing the occurrence of dental caries.","PeriodicalId":15483,"journal":{"name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135401878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of Accidental Chin Staining Following Silver Diamine Fluoride Application in a 24-Month-Old Girl: A Case Report","authors":"Vipul Chandraprakash Lodha, Laresh N Mistry","doi":"10.7860/jcdr/2023/65801.18360","DOIUrl":"https://doi.org/10.7860/jcdr/2023/65801.18360","url":null,"abstract":"Silver Diamine Fluoride (SDF) is a clear liquid that combines the remineralising effects of fluoride with the antibacterial effects of silver. It is a promising therapeutic agent for managing carious lesions in young children and those with special care needs. However, a major complication of SDF is black staining of the skin, mucosa, lips, tongue, and cheeks if improperly applied. This case report presents a complication following SDF application and its management. A 24-month-old girl presented with decayed teeth in the upper back tooth region of the jaw, and SDF application was performed on tooth no. 54 and 64. However, due to the child’s extremely negative behavior and movement during the application of SDF, staining occurred on the chin and lower lip a few hours later. Watchful observation, patient reassurance, and follow-up led to adequate patient management.","PeriodicalId":15483,"journal":{"name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","volume":"239 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135404140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Type and Screen Method versus Antihuman Globulin Crossmatch in Pretransfusion Testing: A Cross-sectional Study","authors":"D Umesh, A Sivaramakrishnan, V Naveen Kumar, Arumugam Pothipillai","doi":"10.7860/jcdr/2023/65847.18364","DOIUrl":"https://doi.org/10.7860/jcdr/2023/65847.18364","url":null,"abstract":"Introduction: Blood transfusion remains the primary modality of treatment for many serious and common diseases. According to the International Society of Blood Transfusion (ISBT), there are about 349 blood group antigens, out of which only about 25-28 antigens are known to cause acute or delayed type of haemolytic transfusion reactions which could be prevented by Pretransfusion Testing (PTT). Regulated pretransfusion tests include ABO blood grouping, Rh typing, antibody detection, antibody identification and compatibility testing. The purpose of compatibility tests is to demonstrate invitro red cell antigen-antibody reaction. The Antihuman Globulin (AHG) crossmatch testing can assure ABO compatibility between donor and patient blood as well as detect most clinically significant antibodies. Type and Screen (T&amp;S) is a procedure carried out as part of PTT in which the recipient’s blood sample is tested for ABO group, RhD T&amp;S for unexpected antibodies. Aim: To compare T&amp;S method of PTT with AHG crossmatch. Materials and Methods: This cross-sectional study was conducted in the department of Transfusion medicine at Government Royapettah Hospital, Chennai and The Tamil Nadu Dr. MGR Medical University, Chennai, Tamil Nadu, India from June 2012 to December 2013. T&amp;S was performed on 1,040 recipients’ (510 males and 530 females) samples. All these samples were subjected to AHG crossmatch with ABO group and RhD type matched donor samples to assess the compatibility between donor and recipient by using column agglutination technology. Statistical analysis was carried out using Statistical Package for the Social Sciences (SPSS) version 11.0. Results: The prevalence of unexpected antibodies in the recipient population was 1.06%. Among the 1,040 recipients’ blood samples, 11 samples were found to have unexpected antibodies. Out of these 11 samples, 10 showed exact antibodies and the remaining one sample with negative antibody screening was found to be incompatible with AHG crossmatch. The sensitivity and specificity of T&amp;S method in comparison to AHG crossmatch was 87.50% and 99.71%, respectively. Conclusion: The sensitivity and specificity of T&amp;S is as acceptable as AHG crossmatch. However, in view of one sample with false negative antibody screening in the study population, it is imperative to know the phenotyping of Red Blood Cells (RBC) antigens of the native population before getting away with AHG crossmatch.","PeriodicalId":15483,"journal":{"name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135404144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}