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Journal of chromatography. B, Biomedical sciences and applications最新文献

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Studies on neurosteroids XII. Determination of enzymatically formed catechol estrogens and guaiacol estrogens by rat brains using liquid chromatography-mass spectrometry-mass spectrometry. 神经类固醇的研究十二。用液相色谱-质谱-质谱法测定大鼠脑中酶解生成的儿茶酚类雌激素和愈创木酚类雌激素。
Journal of chromatography. B, Biomedical sciences and applications Pub Date : 2000-10-01
K Mitamura, M Yatera, K Shimada
{"title":"Studies on neurosteroids XII. Determination of enzymatically formed catechol estrogens and guaiacol estrogens by rat brains using liquid chromatography-mass spectrometry-mass spectrometry.","authors":"K Mitamura,&nbsp;M Yatera,&nbsp;K Shimada","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Enzymic formations of catechol- and guaiacol-estrogens by rat brains have been investigated using classical- and catechol-estrogens as substrates, respectively. The incubation mixtures were pretreated with liquid-liquid and/or solid-phase extraction, and the products were identified by comparison with authentic samples using liquid chromatography-mass spectrometry (-mass spectrometry) [LC-MS (-MS)]. The enzymic activities were also determined by measuring the formed products with LC-MS.</p>","PeriodicalId":15425,"journal":{"name":"Journal of chromatography. B, Biomedical sciences and applications","volume":"748 1","pages":"89-96"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21917166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Solid-phase microextraction for the analysis of biological samples. 用于生物样品分析的固相微萃取。
Journal of chromatography. B, Biomedical sciences and applications Pub Date : 2000-08-04
G Theodoridis, E H Koster, G J de Jong
{"title":"Solid-phase microextraction for the analysis of biological samples.","authors":"G Theodoridis,&nbsp;E H Koster,&nbsp;G J de Jong","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Solid-phase microextraction (SPME) has been introduced for the extraction of organic compounds from environmental samples. This relatively new extraction technique has now also gained a lot of interest in a broad field of analysis including food, biological and pharmaceutical samples. SPME has a number of advantages such as simplicity, low cost, compatibility with analytical systems, automation and the solvent-free extraction. The last few years, SPME has been combined with liquid chromatography and capillary electrophoresis, besides the generally used coupling to gas chromatography, and has been applied to various biological samples such as, e.g., urine, plasma and hair. The objective of the present paper is a survey of the application of SPME for the analysis of biological samples. Papers about the analysis of biologically active compounds are categorised and reviewed. The impact of SPME on various analytical fields (toxicological, forensic, clinical, biochemical, pharmaceutical, and natural products) is illustrated. The main features of SPME and its modes are briefly described and important aspects about its application for the determination of pharmaceuticals, drugs of abuse and compounds of clinical and toxicological interest are discussed. SPME is compared with other sample pretreatment techniques. The potential of SPME and its main advantages are demonstrated. Special attention is paid to new trends in applications of SPME in bioanalysis.</p>","PeriodicalId":15425,"journal":{"name":"Journal of chromatography. B, Biomedical sciences and applications","volume":"745 1","pages":"49-82"},"PeriodicalIF":0.0,"publicationDate":"2000-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21830967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of homocysteine by gas chromatography-mass spectrometry following treatment with chloroformates: a comment. 氯甲酸盐处理后气相色谱-质谱法测定同型半胱氨酸:评述。
Journal of chromatography. B, Biomedical sciences and applications Pub Date : 2000-04-14
P Husek
{"title":"Determination of homocysteine by gas chromatography-mass spectrometry following treatment with chloroformates: a comment.","authors":"P Husek","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15425,"journal":{"name":"Journal of chromatography. B, Biomedical sciences and applications","volume":"740 2","pages":"289-90"},"PeriodicalIF":0.0,"publicationDate":"2000-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21667043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Affinity chromatography on immobilized "biomimetic" ligands. Synthesis, immobilization and chromatographic assessment of an immunoglobulin G-binding ligand. 固定化“仿生”配体的亲和层析。免疫球蛋白g结合配体的合成、固定化及色谱评价。
Journal of chromatography. B, Biomedical sciences and applications Pub Date : 2000-03-31
S F Teng, K Sproule, A Husain, C R Lowe
{"title":"Affinity chromatography on immobilized \"biomimetic\" ligands. Synthesis, immobilization and chromatographic assessment of an immunoglobulin G-binding ligand.","authors":"S F Teng,&nbsp;K Sproule,&nbsp;A Husain,&nbsp;C R Lowe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A synthetic bifunctional ligand (22/8) comprising a triazine scaffold substituted with 3-aminophenol (22) and 4-amino-1-naphthol (8) has been designed, synthesised, characterised and immobilized on agarose beads to create a robust, highly selective affinity adsorbent for human immunoglobulin G (IgG). Scatchard analysis of the binding isotherm for IgG on immobilized 22/8 (90 micromol 22/8/g moist weight gel) indicated an affinity constant (Ka) of 1.4 x 10(5) M(-1) and a theoretical maximum capacity of 151.9 mg IgG/g moist weight gel. The adsorbent shows similar selectivity to immobilized protein A and binds IgG from a number of species. An apparent capacity of 51.9 mg human IgG/g moist weight gel was observed under the experimental conditions selected for adsorption. Human IgG was eluted with glycine-HCl buffer with a recovery of 67-69% and a purity of 97.3-99.2%, depending on the pH value of the buffer used for elution. Preparative chromatography of IgG from human plasma showed that under the specified conditions, 94.4% of plasma IgG was adsorbed and 60% subsequently eluted with a purity of 92.5%. The immobilized ligand was able to withstand incubation in 1 M NaOH for 7 days without loss of binding capacity for IgG.</p>","PeriodicalId":15425,"journal":{"name":"Journal of chromatography. B, Biomedical sciences and applications","volume":"740 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2000-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21646458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular imprinting for drug bioanalysis. A review on the application of imprinted polymers to solid-phase extraction and binding assay. 分子印迹在药物生物分析中的应用。印迹聚合物在固相萃取和结合分析中的应用综述。
Journal of chromatography. B, Biomedical sciences and applications Pub Date : 2000-02-28
L I Andersson
{"title":"Molecular imprinting for drug bioanalysis. A review on the application of imprinted polymers to solid-phase extraction and binding assay.","authors":"L I Andersson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Molecularly imprinted polymers have been applied as selective sorbents in several analytical techniques, including liquid chromatography, capillary electrophoresis and capillary electrochromatography, solid-phase extraction, and 'immunoassay'. An advantage of this type of sorbent is the possibility to synthesize polymers with selectivity pre-determined for a particular analyte. This review critically discusses the use of imprinted polymers for analysis of drugs and other compounds in biological samples, with emphasis on their use as highly selective solid-phase extraction sorbents for sample pre-concentration and alternative binding entities in immunoassay type protocols.</p>","PeriodicalId":15425,"journal":{"name":"Journal of chromatography. B, Biomedical sciences and applications","volume":"739 1","pages":"163-73"},"PeriodicalIF":0.0,"publicationDate":"2000-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21595107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Routine and sensitive method for determination of nicorandil in human plasma developed for liquid chromatography with ultraviolet and mass spectrometric detection. 建立了人血浆中尼可地尔的常规灵敏液相色谱-紫外-质谱检测方法。
Journal of chromatography. B, Biomedical sciences and applications Pub Date : 1999-11-26
S Andrensek, A Smidovnik, A Pecavar, M Prosek
{"title":"Routine and sensitive method for determination of nicorandil in human plasma developed for liquid chromatography with ultraviolet and mass spectrometric detection.","authors":"S Andrensek,&nbsp;A Smidovnik,&nbsp;A Pecavar,&nbsp;M Prosek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A rapid and sensitive method using HPLC has been developed for the quantification of nicorandil (SG-75) in human plasma samples for routine bioequivalence studies. The sample preparation needs two liquid-liquid extractions, first with CH3Cl and HClO4 as denaturation reagent and second with addition of ethyl acetate and Na2CO3(aq). Detection wavelength was 256 nm. The obtained correlation coefficient for weighted linear curve in the range from 5.0 to 300 ng/ml was higher than 0.9950. The limit of quantitation (LOQ) was established at 5.0 ng/ml. The HPLC separation was accomplished on Nucleosil Phenyl (5 microm) stainless steel column within 7 min. The mixture of 0.01 M ammonium acetate buffer (pH 6.2) and acetonitrile 10:3 (v/v) was used as the mobile phase. The same separation method was examined on HPLC-MS system. Using this system, the LOQ was established at 1.0 ng/ml and the linearity was obtained in the range from 1.0 to 150 ng/ml.</p>","PeriodicalId":15425,"journal":{"name":"Journal of chromatography. B, Biomedical sciences and applications","volume":"735 1","pages":"103-9"},"PeriodicalIF":0.0,"publicationDate":"1999-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21486764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel sensitive high-performance liquid chromatographic method for assay of coumarin 7-hydroxylation. 新型灵敏高效液相色谱法测定香豆素7-羟基化。
Journal of chromatography. B, Biomedical sciences and applications Pub Date : 1999-10-29
P Soucek
{"title":"Novel sensitive high-performance liquid chromatographic method for assay of coumarin 7-hydroxylation.","authors":"P Soucek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this paper, a novel HPLC-based method with fluorometric detection of coumarin 7-hydroxylase is presented. The described method provides a time-effective, more sensitive and specific alternative to the previously used spectrofluorometric assay. Using the developed method, metabolism of coumarin in 11 samples of human liver microsomes was evaluated and 1790+/-690 pmol/min/nmol cytochrome P450 (CYP) activity was found. Kinetic parameters and linearity of coumarin 7-hydroxylation were studied in a reconstituted system consisting of recombinant CYP2A6 expressed in Escherichia coli, rat NADPH-CYP reductase and usual components. It was found that a 3.5 to 30 min time of incubation is suitable for estimation of coumarin 7-hydroxylase activity. Observed Km and Vmax values in the CYP2A6 reconstituted system were 1.48+/-0.37 microM and 3360+/-180 pmol product/min/nmol CYP, respectively.</p>","PeriodicalId":15425,"journal":{"name":"Journal of chromatography. B, Biomedical sciences and applications","volume":"734 1","pages":"23-9"},"PeriodicalIF":0.0,"publicationDate":"1999-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21432679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aptamer affinity chromatography: combinatorial chemistry applied to protein purification. 适体亲和层析:用于蛋白质纯化的组合化学。
Journal of chromatography. B, Biomedical sciences and applications Pub Date : 1999-08-20
T S Romig, C Bell, D W Drolet
{"title":"Aptamer affinity chromatography: combinatorial chemistry applied to protein purification.","authors":"T S Romig,&nbsp;C Bell,&nbsp;D W Drolet","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The systematic evolution of ligands by exponential enrichment process is a combinatorial chemistry method that allows the identification of specific oligonucleotide sequences, known as aptamers, that bind to a desired target molecule with high affinity and specificity. Here, a DNA-aptamer specific for human L-selectin was immobilized to a chromatography support to create an affinity column. This column was effectively applied as either the first or second step in the purification of a recombinant human L-selectin-Ig fusion protein from Chinese hamster ovary cell-conditioned medium. The fusion protein was efficiently bound to the column and efficiently eluted by gentle elution schemes. Application of the aptamer column as the initial purification step resulted in a 1500-fold purification with an 83% single step recovery. These results demonstrate that oligonucleotide aptamers can be effective affinity purification reagents.</p>","PeriodicalId":15425,"journal":{"name":"Journal of chromatography. B, Biomedical sciences and applications","volume":"731 2","pages":"275-84"},"PeriodicalIF":0.0,"publicationDate":"1999-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21372602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cumulative author, subject, compound and special issue indexes. Volumes 701-725 (1998-1999). 累积的作者、主题、复合和专刊索引。卷701-725(1998-1999)。
Journal of chromatography. B, Biomedical sciences and applications Pub Date : 1999-07-23
{"title":"Cumulative author, subject, compound and special issue indexes. Volumes 701-725 (1998-1999).","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15425,"journal":{"name":"Journal of chromatography. B, Biomedical sciences and applications","volume":"725 2","pages":"139-291"},"PeriodicalIF":0.0,"publicationDate":"1999-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21348095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification of a highly modified RNA-aptamer. Effect of complete denaturation during chromatography on product recovery and specific activity. 高修饰rna适配体的纯化。色谱过程中完全变性对产物回收率和比活性的影响。
Journal of chromatography. B, Biomedical sciences and applications Pub Date : 1999-04-16
P Bridonneau, S Bunch, R Tengler, K Hill, J Carter, W Pieken, D Tinnermeier, R Lehrman, D W Drolet
{"title":"Purification of a highly modified RNA-aptamer. Effect of complete denaturation during chromatography on product recovery and specific activity.","authors":"P Bridonneau,&nbsp;S Bunch,&nbsp;R Tengler,&nbsp;K Hill,&nbsp;J Carter,&nbsp;W Pieken,&nbsp;D Tinnermeier,&nbsp;R Lehrman,&nbsp;D W Drolet","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To evaluate RNA-aptamers as potential drug candidates, efficient and scaleable purification protocols are needed. Because aptamers are highly structured and rigid molecules, denaturation during the purification process is a critical aspect to obtain a pure and active product. A two-step chromatographic procedure was developed to purify a synthetic anti-VEGF aptamer at the preparative scale. A reversed-phase chromatographic step was optimized with a highly hydrophobic ion pairing reagent, followed by ion-exchange chromatography in which heat and a chaotropic salt were used. Because of the presence of 2'-modified ribose, denaturation conditions had to be optimized in both chromatographic steps to achieve a fully active molecule.</p>","PeriodicalId":15425,"journal":{"name":"Journal of chromatography. B, Biomedical sciences and applications","volume":"726 1-2","pages":"237-47"},"PeriodicalIF":0.0,"publicationDate":"1999-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21217612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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