Japanese Journal of Zoo and Wildlife Medicine最新文献

{"title":"The Passage Time of Prey Through the Digestive Tract of Indo-Pacific Bottlenose Dolphin (Tursiops aduncus)","authors":"R. Takahashi, Nozomi Kobayashi, Suguru Higa, Mai Sakai","doi":"10.5686/jjzwm.26.143","DOIUrl":"https://doi.org/10.5686/jjzwm.26.143","url":null,"abstract":"","PeriodicalId":153831,"journal":{"name":"Japanese Journal of Zoo and Wildlife Medicine","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133309984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respiratory Fungal Infections in Bottlenose Dolphins (Tursiops truncatus)","authors":"Y. Ohno, Y. Inoshima","doi":"10.5686/jjzwm.26.87","DOIUrl":"https://doi.org/10.5686/jjzwm.26.87","url":null,"abstract":"Bottlenose dolphins (Tursiops truncatus) are one of the most popular dolphin species housed in aquaria in Japan. Previous studies have demonstrated that respiratory infections, mainly caused by bacteria and occasionally fungi, are one of the most common diseases in this species. Both Candida spp. and Aspergillus spp. are the most important species as the cause of respiratory fungal infections for this species in Japan. Since these fungi are ubiquitous in the environment surrounding dolphins and aquaria, and the spores may scatter easily in their habitats (poolside), it is very difficult to remove these fungi from their habitats. Therefore, in aquaria in Japan, preventing respiratory fungal infections and treating these infections appropriately in this species including in pregnant and lactating animals and their calves are necessary to maintain the health and number of the current population; this approach must also be applied for respiratory bacterial infections. In this review, to gain a better understanding regarding respiratory fungal infections in this species, the current status of these infections and results of clinical studies in the Port of Nagoya Public Aquarium (PNPA) are described. Moreover, future issues that have been revealed as a result of treating these infections in this species including in pregnant and lactating animals and their calves in the PNPA and that need to be solved are described.","PeriodicalId":153831,"journal":{"name":"Japanese Journal of Zoo and Wildlife Medicine","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127094263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research and Outcome to Solve Issues Facing the Zoo Activity","authors":"K. Takehana","doi":"10.5686/jjzwm.26.75","DOIUrl":"https://doi.org/10.5686/jjzwm.26.75","url":null,"abstract":"","PeriodicalId":153831,"journal":{"name":"Japanese Journal of Zoo and Wildlife Medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125654885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Microsatellite Markers for Paternity Analysis in Captive Loggerhead Turtles (Caretta caretta)","authors":"R. Tadano, M. Mori, Ken Sakaoka, M. Kurita","doi":"10.5686/jjzwm.26.81","DOIUrl":"https://doi.org/10.5686/jjzwm.26.81","url":null,"abstract":"In order to avoid inbreeding, it is important to verify parentage of animals kept in zoos and aquariums. However, determination of parentage is difficult in the case that one female had opportunities of matings by a number of males. In this situation, DNA parentage testing provides reliable results. Microsatellite markers are cost-effective tools for parentage testing and are widely used. In the Port of Nagoya Public Aquarium, Nagoya, Japan, one male (Individual ID: CcW_07) and one female (Individual ID: Cc97_11) of loggerhead turtle (Caretta caretta) had been kept in the same tank since 28 March 2019 in order to breed in captivity. However, mating behavior of CcW_07 could not be observed. Therefore, on 9 May 2020, another male (Individual ID: Cc95_25) instead of CcW_07 was kept together with Cc97_11. However, mating behavior of Cc95_25 could not be confirmed as was the case with CcW_07. After that, Cc97_11 laid eggs on 28 May (Clutch No.1), 14 June (Clutch No.2), and 30 June (Clutch No.3), 2020. Since female turtle has an ability to store sperm within an oviduct over a long period, paternity of these offsprings was unclear in this situation. Therefore, in this study, we applied previously developed microsatellite markers for their paternity examination. Blood samples were collected from two candidate fathers (CcW_07 and Cc95_25), mother (Cc97_11), and 40 offsprings from three clutches. DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). Fourteen microsatellite markers were used for genotyping (Table 1). Based on the degree of polymorphism, these markers were selected from a previous study of loggerhead turtle [1]. The forward primer for each marker was labelled with fluorescent dye. PCR was conducted in 13 μ l reaction volumes containing 12 ng DNA, 1 × GeneAmp PCR Buffer (Applied Biosystems, Foster City, CA, USA), 0.2 mM of each dNTP (Applied Biosystems), 6.25 pmol of each primer, and 0.3 U of AmpliTaq Gold DNA Polymerase (Applied Biosystems). Thermal cycling conditions consisted of: initial denaturation at 95°C for 10 min, followed by 35 cycles of denaturation at 95°C for 1 min, annealing at 55°C for 1 min, and extension at 72°C for 1 min, with a final extension at 72°C for 10 min. Capillary electrophoresis of PCR products was conducted using an ABI PRISM 3500 Genetic Analyzer (Applied Biosystems). Fragment size was determined based on the GeneScan 500 LIZ Size Standard (Applied Biosystems) using GeneMapper software 6 (Applied Biosystems). The number of alleles, observed heterozygosity (HO), unbiased expected * Corresponding author:Ryo TADANO (E-mail: tadano@gifu-u.ac.jp) ABSTRACT This study evaluates the utility of previously developed microsatellite markers in a paternity test of captive loggerhead turtles (Caretta caretta). Two candidate males, one female, and 40 offsprings from three distinct clutches were genotyped for 14 markers. The number of alleles and observed heterozygosity per locus ranged from three to f","PeriodicalId":153831,"journal":{"name":"Japanese Journal of Zoo and Wildlife Medicine","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130856381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Status and Issues, and Challenges toward the Solutions of Aquarium Medicine in Oga Aquarium GAO","authors":"K. Ono","doi":"10.5686/jjzwm.26.63","DOIUrl":"https://doi.org/10.5686/jjzwm.26.63","url":null,"abstract":"","PeriodicalId":153831,"journal":{"name":"Japanese Journal of Zoo and Wildlife Medicine","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115901978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"研究する動物園12：特集論文の趣旨説明","authors":"伸英 木戸, 大 福井","doi":"10.5686/jjzwm.26.61","DOIUrl":"https://doi.org/10.5686/jjzwm.26.61","url":null,"abstract":"","PeriodicalId":153831,"journal":{"name":"Japanese Journal of Zoo and Wildlife Medicine","volume":"39 11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122372492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Status and Issues of Animal Health Management and Medical Care, and Challenges to the Improvement in SENDAI UMINO-MORI AQUARIUM","authors":"Yusuke Tanaka","doi":"10.5686/jjzwm.26.69","DOIUrl":"https://doi.org/10.5686/jjzwm.26.69","url":null,"abstract":"","PeriodicalId":153831,"journal":{"name":"Japanese Journal of Zoo and Wildlife Medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130379248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species Identification of Captive Gibbons in Japan by Using Mitochondrial DNA （cytochrome b） Sequences","authors":"K. Matsudaira, Yoichi Maeda, Naoko Shidehara, T. Ishida","doi":"10.5686/jjzwm.26.35","DOIUrl":"https://doi.org/10.5686/jjzwm.26.35","url":null,"abstract":"","PeriodicalId":153831,"journal":{"name":"Japanese Journal of Zoo and Wildlife Medicine","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121328667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Epidemic Case of Rabbit Hemorrhagic Disease Caused by Rabbit Hemorrhagic Disease Virus 2 （Lagovirus europaeus GI.2） in Tochigi, Japan","authors":"Minato Ueda, Mei Harahuji, Yukiji Shirai, S. Yoneyama, Kaori Tosaki, A. Miyazaki","doi":"10.5686/jjzwm.26.53","DOIUrl":"https://doi.org/10.5686/jjzwm.26.53","url":null,"abstract":"","PeriodicalId":153831,"journal":{"name":"Japanese Journal of Zoo and Wildlife Medicine","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122450224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Determination of Zoonotic Microsporidia from Feral Pigeon (Columba livia) in Japan","authors":"N. Itoh, S. Kameshima, Y. Kimura","doi":"10.5686/jjzwm.26.47","DOIUrl":"https://doi.org/10.5686/jjzwm.26.47","url":null,"abstract":"To determine the prevalence of zoonotic Microsporidia from feral pigeon (Columba livia) in Japan, a total of 108 feces, which were collected in prefectures of Chiba and Nagano, was examined using polymerase chain reaction. Overall, two species of Microsporidia belonging to genus Encephalitozoon were detected from six cases (5.6%: 6/108). In details, E. hellem was determined in one case (0.9%: 1/108) and E. cuniculi was confirmed in three cases (2.8%: 3/108). In addition, coinfection of E. hellem and E. cuniculi was demonstrated in two cases (1.9%: 2/108). According to genotyping analysis, all isolates of E. hellem and E. cuniculi were identified as genotype 2B and genotype Ⅲ , respectively, due to the consistency of DNA base pairs. It is likely to be low risk that the feral pigeon plays a role of reservoir for human Microsporidia infection in Japan","PeriodicalId":153831,"journal":{"name":"Japanese Journal of Zoo and Wildlife Medicine","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123982132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}