Journal of Comparative Physiology B最新文献_第5页

Molecular characterization of myostatin from the skeletal muscle of the African lungfish, Protopterus annectens, and changes in its mRNA and protein expression levels during three phases of aestivation. 非洲肺鱼（Protopterus annectens）骨骼肌肌生长抑制素的分子特征及其mRNA和蛋白表达水平在冬眠三个阶段的变化。

Journal of Comparative Physiology B Pub Date : 2017-05-01 Epub Date: 2017-02-09 DOI: 10.1007/s00360-017-1057-x

Jasmine L Y Ong, You R Chng, Biyun Ching, Xiu L Chen, Kum C Hiong, Wai P Wong, Shit F Chew, Yuen K Ip

{"title":"Molecular characterization of myostatin from the skeletal muscle of the African lungfish, Protopterus annectens, and changes in its mRNA and protein expression levels during three phases of aestivation.","authors":"Jasmine L Y Ong, You R Chng, Biyun Ching, Xiu L Chen, Kum C Hiong, Wai P Wong, Shit F Chew, Yuen K Ip","doi":"10.1007/s00360-017-1057-x","DOIUrl":"10.1007/s00360-017-1057-x","url":null,"abstract":"African lungfishes can aestivate and remain torpid without food and water for years, but disuse muscle atrophy is not prominent during aestivation. This study aimed to clone myostatin (mstn/Mstn), a factor associated with disuse muscle atrophy in mammals, from the skeletal muscle of the African lungfish Protopterus annectens, and to determine its mRNA expression level and protein abundance therein during the induction, maintenance, and arousal phases of aestivation. The complete coding cDNA sequence of mstn comprised 1128 bp, encoding for 376 amino acids with an estimated molecular mass of 42.9 kDa. It was grouped together with Mstn/MSTN of coelacanth and tetrapods in a clade separated from teleost Mstn. After 6 days (the induction phase) of aestivation, the mstn transcript level in the muscle increased significantly, while the protein abundance of Mstn remained comparable to the control. Following that, a significant increase in the expression levels of mstn/Mstn occurred on day 12 (the early maintenance phase) of aestivation. After 6 months of aestivation (the prolonged maintenance phase), the expression levels of mstn/Mstn returned to control levels, indicating the possible impediment of a drastic increase in muscle degradation to prevent muscle atrophy. During 1-3 days of arousal from aestivation, the expression levels of mstn/Mstn in the muscle remained comparable to the control. Hence, tissue reconstruction/regeneration of certain organs might not involve the mobilization of amino acids from the muscle during the early arousal. These results provide insights into how aestivating P. annectens regulates the expression of mstn/Mstn possibly to ameliorate disuse muscle atrophy.","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"182 1","pages":"575-589"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80338880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic plasticity in the common garden snail: big guts and heavier mucus glands compete in snails faced with the dual challenge of poor diet and coarse substrate. 普通园林蜗牛的表型可塑性：面对不良饮食和粗大基质的双重挑战，蜗牛的大肠道和较重的粘液腺相互竞争。

Journal of Comparative Physiology B Pub Date : 2017-05-01 Epub Date: 2016-12-26 DOI: 10.1007/s00360-016-1051-8

Adam J Munn, Marguerite Treloar

{"title":"Phenotypic plasticity in the common garden snail: big guts and heavier mucus glands compete in snails faced with the dual challenge of poor diet and coarse substrate.","authors":"Adam J Munn, Marguerite Treloar","doi":"10.1007/s00360-016-1051-8","DOIUrl":"10.1007/s00360-016-1051-8","url":null,"abstract":"Phenotypic plasticity allows animals to manage environmental challenges. Studies aimed at quantifying plasticity often focus on one challenge, such as diet, and one organ system, such the gastrointestinal tract, but this approach may not adequately reflect how plasticity could buffer multiple challenges. Thus, we investigated the outcomes of a dual challenge experiment that fed land snails either a high-fibre (low quality) or a low-fibre (high quality) diet, and simultaneously exercised them daily over 1.2 m on either a smooth surface of polyvinyl chloride (PVC) or a rough sandpaper. By the end of 20 days, snails fed the poor quality diet had a longer crop and oesophagus and a heavier intestine and rectum than those offered a low-fibre diet. Additionally, high-fibre fed snails had a smaller spermoviduct and oviduct. When also exercised on sandpaper, high-fibre fed snails had a smaller digestive gland, a main energy store, than those exercised on PVC. All snails exercised on sandpaper had a heavier pedal mucus gland, used a loping gait and used less mucus than those on PVC plastic, but there was no difference in the average speed of snails on either surface, supporting the conclusion that loping is a mucus conserving gait. Notably, snails faced with both a diet and substrate challenge had a smaller kidney, which could directly effect fecundity. This demonstrates that our dual challenge approach has potential for evaluating the costs and limits of the plasticity necessary to fully appreciate the evolutionary significance of plasticity in snails and other species.","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"70 1","pages":"545-561"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77462269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intra-population variability of ocean acidification impacts on the physiology of Baltic blue mussels (Mytilus edulis): integrating tissue and organism response. 海洋酸化对波罗的海蓝贻贝（Mytilus edulis）生理影响的种群内变异：整合组织和生物反应。

Journal of Comparative Physiology B Pub Date : 2017-05-01 Epub Date: 2016-12-05 DOI: 10.1007/s00360-016-1053-6

L S Stapp, J Thomsen, H Schade, C Bock, F Melzner, H O Pörtner, G Lannig

{"title":"Intra-population variability of ocean acidification impacts on the physiology of Baltic blue mussels (Mytilus edulis): integrating tissue and organism response.","authors":"L S Stapp, J Thomsen, H Schade, C Bock, F Melzner, H O Pörtner, G Lannig","doi":"10.1007/s00360-016-1053-6","DOIUrl":"10.1007/s00360-016-1053-6","url":null,"abstract":"Increased maintenance costs at cellular, and consequently organism level, are thought to be involved in shaping the sensitivity of marine calcifiers to ocean acidification (OA). Yet, knowledge of the capacity of marine calcifiers to undergo metabolic adaptation is sparse. In Kiel Fjord, blue mussels thrive despite periodically high seawater PCO2, making this population interesting for studying metabolic adaptation under OA. Consequently, we conducted a multi-generation experiment and compared physiological responses of F1 mussels from 'tolerant' and 'sensitive' families exposed to OA for 1 year. Family classifications were based on larval survival; tolerant families settled at all PCO2 levels (700, 1120, 2400 µatm) while sensitive families did not settle at the highest PCO2 (≥99.8% mortality). We found similar filtration rates between family types at the control and intermediate PCO2 level. However, at 2400 µatm, filtration and metabolic scope of gill tissue decreased in tolerant families, indicating functional limitations at the tissue level. Routine metabolic rates (RMR) and summed tissue respiration (gill and outer mantle tissue) of tolerant families were increased at intermediate PCO2, indicating elevated cellular homeostatic costs in various tissues. By contrast, OA did not affect tissue and routine metabolism of sensitive families. However, tolerant mussels were characterised by lower RMR at control PCO2 than sensitive families, which had variable RMR. This might provide the energetic scope to cover increased energetic demands under OA, highlighting the importance of analysing intra-population variability. The mechanisms shaping such difference in RMR and scope, and thus species' adaptation potential, remain to be identified.","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"647 1","pages":"529-543"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74737982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Swimming metabolic rates vary by sex and development stage, but not by species, in three species of Australian otariid seals. 在三种澳大利亚耳海豹中，游泳代谢率因性别和发育阶段而异，但不因物种而异。

Journal of Comparative Physiology B Pub Date : 2017-04-01 Epub Date: 2016-11-01 DOI: 10.1007/s00360-016-1046-5

Monique A Ladds, David J Slip, Robert G Harcourt

{"title":"Swimming metabolic rates vary by sex and development stage, but not by species, in three species of Australian otariid seals.","authors":"Monique A Ladds, David J Slip, Robert G Harcourt","doi":"10.1007/s00360-016-1046-5","DOIUrl":"10.1007/s00360-016-1046-5","url":null,"abstract":"Physiology may limit the ability for marine mammals to adapt to changing environments. Depth and duration of foraging dives are a function of total available oxygen stores, which theoretically increase as animals grow, and metabolic costs. To evaluate how physiology may influence the travelling costs for seals to foraging patches in the wild, we measured metabolic rates of a cross-section of New Zealand fur seals, Australian fur seals and Australian sea lions representing different foraging strategies, development stages, sexes and sizes. We report values for standard metabolic rate, active metabolic rate (obtained from submerged swimming), along with estimates of cost of transport (COT), measured via respirometry. We found a decline in mass-specific metabolic rate with increased duration of submerged swimming. For most seals mass-specific metabolic rate increased with speed and for all seals mass-specific COT decreased with speed. Mass-specific metabolic rate was higher for subadult than adult fur seals and sea lions, corresponding to an overall higher minimum COT. Some sex differences were also apparent, such that female Australian fur seals and Australian sea lions had higher mass-specific metabolic rates than males. There were no species differences in standard or active metabolic rates for adult males or females. The seals in our study appear to operate at their physiological optimum during submerged swimming. However, the higher metabolic rates of young and female fur seals and sea lions may limit their scope for increasing foraging effort during times of resource limitation.","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"1 1","pages":"503-516"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74232831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Di- and tripeptide transport in vertebrates: the contribution of teleost fish models. 脊椎动物的二肽和三肽运输：硬骨鱼模型的贡献。

Journal of Comparative Physiology B Pub Date : 2017-04-01 Epub Date: 2016-11-01 DOI: 10.1007/s00360-016-1044-7

Tiziano Verri, Amilcare Barca, Paola Pisani, Barbara Piccinni, Carlo Storelli, Alessandro Romano

{"title":"Di- and tripeptide transport in vertebrates: the contribution of teleost fish models.","authors":"Tiziano Verri, Amilcare Barca, Paola Pisani, Barbara Piccinni, Carlo Storelli, Alessandro Romano","doi":"10.1007/s00360-016-1044-7","DOIUrl":"10.1007/s00360-016-1044-7","url":null,"abstract":"Solute Carrier 15 (SLC15) family, alias H+-coupled oligopeptide cotransporter family, is a group of membrane transporters known for their role in the cellular uptake of di- and tripeptides (di/tripeptides) and peptide-like molecules. Of its members, SLC15A1 (PEPT1) chiefly mediates intestinal absorption of luminal di/tripeptides from dietary protein digestion, while SLC15A2 (PEPT2) mainly allows renal tubular reabsorption of di/tripeptides from ultrafiltration, SLC15A3 (PHT2) and SLC15A4 (PHT1) possibly interact with di/tripeptides and histidine in certain immune cells, and SLC15A5 has unknown function. Our understanding of this family in vertebrates has steadily increased, also due to the surge of genomic-to-functional information from 'non-conventional' animal models, livestock, poultry, and aquaculture fish species. Here, we review the literature on the SLC15 transporters in teleost fish with emphasis on SLC15A1 (PEPT1), one of the solute carriers better studied amongst teleost fish because of its relevance in animal nutrition. We report on the operativity of the transporter, the molecular diversity, and multiplicity of structural-functional solutions of the teleost fish orthologs with respect to higher vertebrates, its relevance at the intersection of the alimentary and osmoregulative functions of the gut, its response under various physiological states and dietary solicitations, and its possible involvement in examples of total body plasticity, such as growth and compensatory growth. By a comparative approach, we also review the few studies in teleost fish on SLC15A2 (PEPT2), SLC15A4 (PHT1), and SLC15A3 (PHT2). By representing the contribution of teleost fish to the knowledge of the physiology of di/tripeptide transport and transporters, we aim to fill the gap between higher and lower vertebrates.","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"67 1","pages":"395-462"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87580331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of melanocortin-4 receptor agonists and antagonists on expression of genes related to reproduction in spotted scat, Scatophagus argus

Journal of Comparative Physiology B Pub Date : 2017-02-14 DOI: 10.1007/s00360-017-1062-0

Dongneng Jiang, Jiantao Li, Y. Tao, Hua-pu Chen, S. Deng, Chun-hua Zhu, Guang-Li Li

引用次数: 1

Functional characterization of a novel disaccharide transporter in lobster hepatopancreas 龙虾肝胰脏中一种新型双糖转运体的功能表征

Journal of Comparative Physiology B Pub Date : 2017-02-08 DOI: 10.1007/s00360-017-1058-9

Olivia Scheffler, G. Ahearn

引用次数: 3

Trade-offs between reproduction and health in free-ranging African striped mice 自由放养的非洲条纹鼠繁殖与健康之间的权衡

Journal of Comparative Physiology B Pub Date : 2017-02-04 DOI: 10.1007/s00360-016-1054-5

I. Schoepf, Neville Pillay, C. Schradin

引用次数: 6

Detoxification and elimination of nicotine by nectar-feeding birds 以花蜜为食的鸟类对尼古丁的解毒和消除作用

Journal of Comparative Physiology B Pub Date : 2017-02-01 DOI: 10.1007/s00360-016-1055-4

S. Lerch-Henning, E. E. du Rand, S. W. Nicolson

引用次数: 2

Determinants of coronary blood flow in sandbar sharks, Carcharhinus plumbeus. 沙洲鲨鱼冠状动脉血流的决定因素。

Journal of Comparative Physiology B Pub Date : 2017-02-01 Epub Date: 2016-09-27 DOI: 10.1007/s00360-016-1033-x

Georgina K Cox, Richard W Brill, Kaitlin A Bonaro, Anthony P Farrell

{"title":"Determinants of coronary blood flow in sandbar sharks, Carcharhinus plumbeus.","authors":"Georgina K Cox, Richard W Brill, Kaitlin A Bonaro, Anthony P Farrell","doi":"10.1007/s00360-016-1033-x","DOIUrl":"10.1007/s00360-016-1033-x","url":null,"abstract":"The coronary circulation first appeared in the chordate lineage in cartilaginous fishes where, as in birds and mammals but unlike most teleost fishes, it supplies arterial blood to the entire myocardium. Despite the pivotal position of elasmobranch fishes in the evolution of the coronary circulation, the determinants of coronary blood flow have never been investigated in this group. Elasmobranch fishes are of special interest because of the morphological arrangement of their cardiomyocytes. Unlike teleosts, the majority of the ventricular myocardium in elasmobranch fishes is distant to the venous blood returning to the heart (i.e., the luminal blood). Also, the majority of the myocardium is in close association with the coronary circulation. To determine the relative contribution of the coronary and luminal blood supplies to cardiovascular function in sandbar sharks, Carcharhinus plumbeus, we measured coronary blood flow while manipulating cardiovascular status using acetylcholine and adrenaline. By exploring inter- and intra-individual variation in cardiovascular variables, we show that coronary blood flow is directly related to heart rate (R 2 = 0.6; P < 0.001), as it is in mammalian hearts. Since coronary blood flow is inversely related to coronary resistance both in vivo and in vitro, we suggest that in elasmobranch fishes, changes in heart rate mediate changes in coronary vascular resistance, which adjust coronary blood flow appropriately.","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"131 1","pages":"315-327"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78043729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0