Molecular & Cellular Biomechanics最新文献

{"title":"Navigating the biomechanical landscape: Enhanced methods for drug analysis","authors":"Shanghui Sun","doi":"10.62617/mcb.v21.130","DOIUrl":"https://doi.org/10.62617/mcb.v21.130","url":null,"abstract":"In drug analysis and detection, chemiluminescence is a standard technical method based on the idea that the concentration of observed substances in the chemical detection system and the chemical luminescence intensity establish a linear quantitative relationship under certain conditions. Bio-mechanical landscape (BML), which uses bioengineered systems to simulate essential elements of the human tumour microenvironment, is already enhancing cancer biology and aiding clinical translation. The majority of illicit substances have dangerous cardiovascular side effects that might range from an irregular heartbeat to a heart attack. Cardiovascular disease, including blocked veins and bacterial infections of the blood arteries and heart valves, may also result from injecting illicit narcotics. The challenging characteristics of such a cardiovascular disease using drug analysis are heart failure, low blood pressure and headaches. Hence, in this research, drug analysis has improved wearable body sensor network-enabled artificial neural network (WBSN-ANN) technologies for cardiovascular disease. Medicine and drug development because they provide information on patient status in the clinical context and vital information on pharmacodynamics activity, effectiveness, and safety throughout the research process. The process results in a research-intensive organization finding a novel chemical or biological compound and developing it into a medicine that patients can legally utilize for drug discovery and development.","PeriodicalId":153642,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"32 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141814263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of Mongolian Medicine Chagan Gaoyou-4 Powder on bone mineral density induced by retinoic acid in rats","authors":"Tubuxin, Seesregdorj Suregjid, Dorjbat Sosorburam","doi":"10.62617/mcb.v21.127","DOIUrl":"https://doi.org/10.62617/mcb.v21.127","url":null,"abstract":"Objective: To investigate the effects of Chagan-Gaoyou-4 powder on spine bone mineral density and serum estrogen and estrogen receptor alpha (ERa) and estrogen receptor beta (ERβ) protein expression in bone tissue induced by retinoic acid in rats. Methods: Sixty 3-month-old SPF female SD rats were randomly divided into model group (retinoin group), normal group (SHAM group), Mongolian medicine group (Chagan-Gaoyou 4 group), and control group (Gushukang granule group). After 14 days of administration, spinal Bone Mineral Density (BMD) was measured, and external manifestations were observed. Results: Compared with the SHAM group, the BMD values of all medication groups decreased (P < 0.05, P < 0.001). Compared with the model group, the spinal BMD value of the Chagan Gaoyou-4 group was increased after 21 days of treatment, and the difference was statistically significant (P < 0.01, P < 0.05). After 21 days, the spinal BMD value of all treatment groups was increased (P < 0.05), the spinal BMD value was also increased, especially Chagan Gaoyou-4 group was more obviously increased (P < 0.05). Compared with the normal group, ERa and ERβ levels in the model group were significantly decreased (P < 0.001), and ERa and ERβ levels in all drug groups were significantly increased (P < 0.001) compared with the model group. After 21 days of treatment, there was no significant difference among the three treatment groups (P > 0.05). ERa and ERβ levels in the Chagan Gaoyou-4 group were higher than those in the model group (P < 0.05), but there was no significant difference between the group and the Gushukang granule group (P > 0.05). The E2 content of the Chagan Gaoyou-4 group was higher than that of the model group (P < 0.05). The mRNA expression of estrogen receptor ERa and ERβ in bone tissue of the left proximal femur was detected by RT-PCR. Compared with the model group, the expression of ERa and ERβ mRNA in the Chagan Gaoyou-4 group and Gushukang granule group was increased. Compared with the model group, ERp mRNA expression was increased in the Chagan Gaoyou-4 powder medium-dose group. Compared with the Gushukang granule group, there was no significant difference in ERm RNA expression in the Chagan Gaoyou-4 dose groups. Chagan Gaoyou-4 can up-regulate the expression of ERa and ERβ induced by retinoic acid in rats, indicating that the Chagan Gaoyou-4 powder group may promote bone formation, regulate bone resorption, improve bone mineral density, and achieve the purpose of preventing and treating OP by increasing estrogen level, stimulating estrogen receptor, and increasing the expression of ERa and ERβ in bone tissue. Conclusion: Chagan-Gaoyu-4 powder may have estrogen-like effects on the bone tissue of rats induced by retinoic acid and may increase the level of serum estrogen, ERa, and ERβ protein expression, thereby improving the spinal BMD of experimental rats. The Chagan-Gaoyou-4 powder group could improve the general condition of osteoporosis induced by r","PeriodicalId":153642,"journal":{"name":"Molecular &amp; Cellular Biomechanics","volume":"9 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141640625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying sequential differences between protein structural classes using network and statistical approaches.","authors":"Xiaogeng Wan, Xinying Tan","doi":"10.62617/mcb.v21.199","DOIUrl":"https://doi.org/10.62617/mcb.v21.199","url":null,"abstract":"Protein sequence information are believed to embed the hint of their structures. In this study, we motivate to use network and statistical approaches to identify the sequential differences between different protein structural classes and between different structural motifs. By examine significant amino acid feature interactions and statistical distributions of feature series, both common and special characteristics are identified for the different protein structural types. Analyses suggest that all top protein structural classes of CATH and SCOP show Leu, Val, and Asn as the sources of strong feature interactions, while Cys, His, Trp, and Met exhibit weak intra-type interactions with other features. There are also significant interactions between amino acids features such as Ala and -helix and bend preference, Ala and side-chain size, Ala and Gly, and Met and Leu. These phenomena are observed in all structural classes, which are assumed to have little influences in distinguishing the different structures. In  structures, Glu, Pro and side-chain size, hydrophobicity exhibit high importance in feature interactions, while in  structures, Gly, Thr and physical properties such as -helix and bend preference, extended structural preference, pK-C value and surrounding hydrophobicity for  structures show high importance in feature interactions. When comparing between the  and  structures, both types of structures show Ser as the common sources of feature interactions. The mixed  and  structures not only present common feature interactions with  and  structures, but exhibit special interactions between Met, Lys and double-bend preference property, and between the sequence arrangements of Cys, His, Met, Tyr and amino acid composition features. The intrinsically disordered proteins (IDPs) tends to present repetition patterns for a same kind of amino acids in high frequency Kmers, while the nine typical types of structural motifs also show different characteristics. Different value ranges are also found for different structural types according to statistical tests. The outcomes of this comparison study not only help to illuminate the mechanism of amino acid feature interactions in different types of structures, but also help us gain deeper understanding on how protein sequence influence structures.","PeriodicalId":153642,"journal":{"name":"Molecular &amp; Cellular Biomechanics","volume":"87 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141835083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of immediate neuromuscular training on ankle biomechanics in individuals with functional ankle instability","authors":"Jiongxiang Zhao, Enze Shao, Julien S. Baker, E. Teo, Yaodong Gu","doi":"10.62617/mcb.v21.162","DOIUrl":"https://doi.org/10.62617/mcb.v21.162","url":null,"abstract":"Functional ankle instability arises from recurrent ankle sprains. Neuromuscular training is employed to enhance ankle stability in individuals who experience functional ankle instability. The study involved 24 male university students with functional ankle instability, undergoing ankle neuromuscular training on three surfaces. The OpenSim musculoskeletal model assessed effects on ankle kinematics, kinetics, and muscle activity. Using one-way repeated measures ANOVA and one-dimensional statistical non-parametric mapping to distinguish differences among training surfaces. The study aimed to compare biomechanical characteristics of ankle motion in individuals with functional ankle instability undergoing immediate neuromuscular training on a foam cushion surface versus training on level-ground and artificial turf. Results showed foam cushion training significantly increased tibialis anterior and gastrocnemius medial activation during walking (p < 0.05), with no differences observed in peak ankle plantarflexion, peroneus longus, and gastrocnemius lateral. Foam cushion training further increased activation in four muscles and peak ankle plantarflexion moment during jogging and fast running (p < 0.05). Furthermore, foam cushion training reduced subtalar mobility (p < 0.05) and showed greater dorsiflexion angles during jogging and fast running (p < 0.05). Therefore, immediate ankle neuromuscular training on a foam cushion is more advantageous in enhancing ankle stability among individuals with functional ankle instability, positively impacting functional ankle instability improvement.","PeriodicalId":153642,"journal":{"name":"Molecular &amp; Cellular Biomechanics","volume":" December","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141669709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the role of lycopene in promoting mammalian spermatogenesis","authors":"Yun Li, Guangzhong Ma, Enzhong Li","doi":"10.62617/mcb.v21.76","DOIUrl":"https://doi.org/10.62617/mcb.v21.76","url":null,"abstract":"A carotenoid called lycopene (LYC) is one of the most potent antioxidants. Superior physiological properties of LYC include cancer prevention, cholesterol reduction, antioxidant activity, scavenging of free radicals, immunity enhancement, prostate protection, and increased sperm viability. In recent times, male sperm quality has decreased. Following studies on LYC’s function in spermatogenesis, the therapy of male infertility diseases has made extensive use of it. Here, we give an accurate theoretical foundation for the use of LYC in large animal breeding and the treatment of male infertility in humans by summarising the variables influencing spermatogenesis and the enhancing effect of LYC on mammalian spermatogenesis.","PeriodicalId":153642,"journal":{"name":"Molecular &amp; Cellular Biomechanics","volume":"42 40","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141270388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WGCNA-based identification of anoikis-related subtypes, prognostic significance, and characterisation of the immune microenvironment in Philadelphia-negative acute lymphoblastic leukaemia","authors":"Na Li, Yang Hong, Ling Zhang, Aining Sun","doi":"10.62617/mcb.v21.90","DOIUrl":"https://doi.org/10.62617/mcb.v21.90","url":null,"abstract":"The clinical outcomes and incidence of Philadelphia chromosome-negative B cell acute lymphoblastic leukaemia (ph-neg B-ALL) vary significantly across different age groups, influencing the prognosis. Despite recent advancements in diagnostic and therapeutic techniques, the detailed prognosis for ph-negative B-ALL across age demographics remains to be elucidated. In this study, clinical data were obtained from 80 patients with ph-neg B-ALL who were diagnosed at our centre. Ribonucleic acid sequencing was performed using their initial bone marrow aspirate samples. By employing weighted gene co-expression network analysis (WGCNA) on 408 anoikis-related genes (ARGs), four different modules were identified and subsequently analysed through bioinformatics. The WGCNA revealed distinct co-expression modules among ARGs. Specifically, the ARGs in the turquoise module might assess the risk associated with newly diagnosed ph-neg B-ALL. Additionally, the study revealed significant heterogeneity in the immune microenvironment and genome variance, highlighting the notable heterogeneity within the disease. 408 ARGs were screened out and four different co-expression modules were constructed by WGCNA algorithms from the RNA-sequencing data of 80 ph-neg B-ALL patients; The ARGs in the turquoise module were the most, and it can be used to divide the de novo ph-neg B-ALL patients to different risk groups(high-risk and low-risk); The ph-neg B-ALL patients can be divided into PS-1 and PS-2, there is heterogeneity of genomes between PS-1 and PS-2; Immune infiltration difference exists in between PS-1 and PS-2. In conclusion, our study holds significant value in exploring the molecular pathways and mechanisms associated with anoikis implicated in ph-neg B-ALL, and in facilitating the development of treatments and prognostic tools for this disease","PeriodicalId":153642,"journal":{"name":"Molecular &amp; Cellular Biomechanics","volume":"24 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141120054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induced fatigue impact on plantar pressure in females with mild hallux valgus","authors":"Shunxiang Gao, Dong Sun, Yang Song, Xuanzhen Cen, Hairong Chen, Yining Xu, Shirui Shao","doi":"10.62617/mcb.v21.135","DOIUrl":"https://doi.org/10.62617/mcb.v21.135","url":null,"abstract":"Fatigue has been established to change plantar pressure distribution, yet its impact on hallux valgus (HV) patients, who exhibit morphological and biomechanical changes in the foot, remains insufficiently studied. Twenty-eight female participants, comprising 16 with mild HV and 12 healthy controls, were recruited. Plantar pressures were recorded pre- and post-fatigue using the Footscan platform during self-selected-speed walking trials, fatigue protocol was performed on a treadmill. Foot was segmented into 10 anatomical regions for calculating parameters including maximal force, peak pressure, impulse, contact duration, contact area, and force time-series, alongside assessing the distribution of medial and lateral contact forces (Foot balance) across the groups. During post-fatigue, patients with mild HV demonstrated adaptive changes in plantar pressure distinct from healthy controls, with significant reductions in maximal force, peak pressure, and impulse in the M1 and M2 regions and increases in the M3–M5 regions. In contrast, the control group exhibited an opposite pattern, concentrating pressure in the M1 and M2 regions post-fatigue. The force time-series analysis revealed significant disparities between HV patients and controls, particularly in the M4 and M5 regions, where HV patients showed a less pronounced and lower passive peak in forces. Results show that women with mild HV demonstrate adaptive changes in plantar pressure post-fatigue, distinctly different from healthy individuals, aiding in preventive strategies for fatigue-induced foot injuries for HV patients.","PeriodicalId":153642,"journal":{"name":"Molecular &amp; Cellular Biomechanics","volume":"40 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141118856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finite element analysis of the biomechanical effects of anterior and posterior cervical fusion surgery for bilateral cervical dislocation","authors":"Dan Li, Ke Wang, Chao Dong, Bo Zhou, Lin Gu, Haoran Yang","doi":"10.62617/mcb.v21.133","DOIUrl":"https://doi.org/10.62617/mcb.v21.133","url":null,"abstract":"Lower cervical spine injuries often manifest as lower cervical vertebral fractures and dislocations, as well as lower cervical facet joint dislocations. Especially in cases of bilateral facet joint dislocations, it is important to rapidly and effectively relieve spinal cord and nerve root compression to prevent secondary spinal cord injury, while also providing reliable and long-lasting stability to the injured segment after surgery. Combined anterior and posterior approaches have the advantages of both pure anterior or posterior approaches, but the actual situation is complex and variable, making systematic theoretical analysis crucial. This study, with bilateral facet joint dislocation of the C6 segment and cervical spinal cord injury as the research background, established a three-dimensional model of the cervical spine C3-C7 after implementing four types of anterior-posterior combined surgeries. The four surgical approaches consist of four combinations: anterior parallel or inclined screw placement combined with posterior Margel or Anderson method screw insertion. Through finite element method, a systematic comparative analysis of the theoretical effects of the four combined surgeries in treating bilateral facet joint dislocation of the cervical spine was conducted. The conclusion was that the variations in the four combined fixation methods have a certain impact on the biomechanical characteristics of the intervertebral disc nucleus. There is a clear mutual influence relationship among anterior and posterior fixation instruments. Based on the model used in this study, it is recommended to use a torque greater than 2.1 nm to tighten the locking nut of the posterior rod to ensure reliable internal fixation.","PeriodicalId":153642,"journal":{"name":"Molecular &amp; Cellular Biomechanics","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141000077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study on the Importance of Core Strength and Coordination Balance during Basketball Based on Biomechanics","authors":"Yixin Liu","doi":"10.32604/mcb.2022.019342","DOIUrl":"https://doi.org/10.32604/mcb.2022.019342","url":null,"abstract":"","PeriodicalId":153642,"journal":{"name":"Molecular &amp; Cellular Biomechanics","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121617161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a Double Helical Spring Decompression Structure Backpack on the Lumbar Spine Biomechanics of School-Age Children: A Finite Element Study","authors":"Fengping Li, Dong Sun, Qiaolin Zhang, Hairong Chen, Istv醤 B韗� Zhiyi Zheng, Yaodong Gu","doi":"10.32604/mcb.2023.041016","DOIUrl":"https://doi.org/10.32604/mcb.2023.041016","url":null,"abstract":"","PeriodicalId":153642,"journal":{"name":"Molecular &amp; Cellular Biomechanics","volume":"103 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124727954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}