Journal of chemical technology and biotechnology最新文献

{"title":"Economically and environmentally efficient triple-column pressure-swing distillation with heat integration and heat pump techniques for the recovery of tetrahydrofuran and n-butanol from waste effluent","authors":"Pengyuan Ding,&nbsp;Shuangshuang Zhu,&nbsp;Ao Yang","doi":"10.1002/jctb.70191","DOIUrl":"https://doi.org/10.1002/jctb.70191","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>The ternary azeotropic tetrahydrofuran, <i>n</i>-butanol, and water system is always produced from the waste effluent of <i>γ</i>-butyrolactone production units. The separation of this system using economically and environmentally efficient processes is essential for environmental protection and resource recovery.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>Two alternative triple-column pressure-swing distillation (TCPSD) processes were developed to separate the ternary azeotropic tetrahydrofuran, <i>n</i>-butanol, and water mixture based on thermodynamic strategies. Then, the process was optimized to obtain the optimal decision variables, with economic and environmental performance metrics adopted as the optimization objectives. Process intensification strategies, including heat integration (HI) and heat pump (HP), were adopted to decrease energy consumption and CO₂ emissions. The results illustrated that HI-assisted TCPSD processes achieve reductions of approximately 11–20% of cost and 15–20% of CO₂ emissions. Furthermore, HP-assisted TCPSD processes yield more substantial improvements, with cost and CO₂ emissions reduced by about 18–22% and 32–40%, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>Overall, TCPSD processes incorporating HI or HP technologies demonstrate superior economic and environmental performance compared to the conventional process. © 2026 Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"101 8","pages":"1591-1601"},"PeriodicalIF":2.7,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148386191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of ammonium concentration on methane oxidation and CO2 removal in an anaerobic biotrickling filter treating synthetic landfill gas and leachate","authors":"Cecilia Lizeth Alvarez-Guzmán,&nbsp;Karla María Muñoz-Páez,&nbsp;Alejandro Vargas,&nbsp;Idania Valdez-Vazquez","doi":"10.1002/jctb.70195","DOIUrl":"https://doi.org/10.1002/jctb.70195","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>Landfill gas (LFG) and leachate are commonly treated separately through energy-intensive processes. This study evaluated the integration of both streams in an anaerobic biotrickling filter (BTF) to achieve simultaneous methane (CH₄) and carbon dioxide (CO₂) removal coupled with ammonium (NH₄⁺) transformation, and how the microbial community mediates these processes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>The BTF was fed with synthetic landfill gas (CH₄/CO₂/N₂, 50:35:15% v/v) and operated under two NH₄⁺ levels (500 and 3700 mg L⁻¹) in the presence of sulfate (SO₄²⁻, 200 mg L⁻¹). At 500 mg NH₄⁺ L⁻¹ (Stage I), simultaneous CH₄ oxidation (16 615.7 ± 5266.2 nmol CH₄ cm⁻³ d⁻¹) and CO₂ removal (5481.6 ± 5000.2 nmol CO₂ cm⁻³ d⁻¹) were achieved, reaching a mean CH₄ and CO₂ removal efficiencies (RE) of 43.9% and 19.9%, respectively, with 37.6% NH₄⁺ removal. At 3700 mg NH₄⁺ L⁻¹ (Stage II), CH₄ elimination capacity (EC) notably dropped (0.5% RE), while CO₂ EC persisted at lower levels (7.5% RE), achieving a value of 2101.7 ± 2433.1 nmol CO₂ cm⁻³ d⁻¹ and 65% NH₄⁺ removal. Microbial analysis revealed a consortium in which <i>Methanosarcina</i> and <i>Methanobacterium</i> potentially mediated reverse methanogenesis. The coexistence of denitrifiers (<i>Caenispirillum</i>, <i>Aquabacterium</i>) and sulfur-cycling bacteria (Desulfobacterota) suggests that C₁ transformations were supported by syntrophic interactions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>This study demonstrates the feasibility of simultaneously treating LFG and leachate in a BTF reactor. NH₄⁺ concentration acted as key driver of reactor performance and metabolic activity, indicating that NH₄⁺ load can be used as a strategic control variable to drive the system functionality, offering an alternative for LFG mitigation. © 2026 The Author(s). <i>Journal of Chemical Technology and Biotechnology</i> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"101 8","pages":"1632-1642"},"PeriodicalIF":2.7,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jctb.70195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148386728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hierarchical ZSM-5/SBA-15 composites supported non-Noble metal oxides for efficient catalytic oxidation of HMF to FDCA","authors":"Zicheng Li,&nbsp;Lei Yang,&nbsp;Tengxin Ren,&nbsp;Lishu Shao,&nbsp;Na Liu,&nbsp;Zhiping Wu,&nbsp;Caixia Zhu,&nbsp;Minghui Tan,&nbsp;Hao Chen,&nbsp;Linlin Zhao,&nbsp;Noritatsu Tsubaki","doi":"10.1002/jctb.70198","DOIUrl":"https://doi.org/10.1002/jctb.70198","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>The efficient utilization of biomass resources is a key pathway to replace fossil fuels. The oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) played an important role for synthesizing bio-based polyester monomers. In this study, a series of ZSM-5/SBA-15 composite molecular sieves were prepared via the simple mechanical grinding method to construct micro-mesoporous supports with pore size of 0.64 ~ 9.31 nm, hierarchical structure, and dual acidic sites. Then, non-noble metal oxides (Co, Fe, Cr, and Mn) were loaded on composite supports by wet impregnation method, respectively. The composite support ZS-21 optimized the dispersion and stability of the active component, accelerated mass transfer, and thereby universally enhanced the catalytic activity of non-noble metal oxides.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>The most pronounced improvement was observed for Cr-ZS-21, which achieved a 77% increase of FDCA yield compared with that of single supports. Meanwhile, 10Co-ZS-21 catalyst showed the best performance with HMF conversion of 96.6% and FDCA yield of 55.1% under optimized conditions (140 °C, 1.0 MPa O₂, 24 h), and FDCA yield remained at 52.98% after five cycles. Based on the catalyst characterization, we considered that the coexisting Co³⁺ and Co²⁺ established a dynamic redox cycle, providing the sustained driving force for the stepwise oxidation. The stable Co-O-Si chemical bonds and the hierarchical pore structure collectively enhanced the catalytic activity and stability.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>This study provided a series of non-noble metal catalysts by a facile synthesis method for the efficient production of bio-based FDCA, which possessed cost advantages and industrial potential. © 2026 Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"101 8","pages":"1559-1575"},"PeriodicalIF":2.7,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148386153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential valorization of waste sludge in the papermaking industry for enhancing paper tensile strength","authors":"Yuntao Tian,&nbsp;Anna Huang,&nbsp;Shiyue Chen,&nbsp;Huaying Zhang,&nbsp;Hassan Ramadan,&nbsp;Zhaoji Zhang","doi":"10.1002/jctb.70193","DOIUrl":"https://doi.org/10.1002/jctb.70193","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Traditional sludge treatment methods face significant challenges, including threats of secondary pollution, high carbon emissions, economic constraints, and limited scalability. These drawbacks have prompted research into alternative solutions for sludge resource utilization. In this study, sludge cakes produced <i>via</i> catalytic wet air oxidation (CWAO) were applied in the papermaking process to replace conventional inorganic fillers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Waterworks sludge (WS) and civil sludge (CS) were found to have the lowest effect on enhancing the tensile strength of the paper, while kitchen waste sludge (KS), paper sludge (PS), and post-membrane thickened liquid sludge (MS) showed superior improvement in tensile strength thanks to their organic composition. The integration of cationic etherified starch (CES) with the three aforementioned sludge types enhanced the tensile strength, with the utmost strength reaching 0.358 kN/m at 3 wt.% CES and 30 wt.% PS. According to SEM characterization, the inorganic components in the fillers filled the voids among the fibers, while the organic components acted as connection links between the fibers and fillers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This is the first study that provides a comprehensive guide for replacing conventional inorganic fillers with CWAO-treated sludge, supported by CES additive, to facilitate resource valorization and cost reduction in the papermaking industry. Despite multiple benefits related to lowering the consumption of conventional fillers and reduced sludge handling costs, this approach is only appropriate for end-use applications that do not involve direct contact with food or medicine, and the addition of CWAO-treated sludge and CES must be determined according to the required tensile strength. © 2026 Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"101 8","pages":"1643-1653"},"PeriodicalIF":2.7,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148386567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental challenges and emerging strategies for sustainable removal of synthetic dyes from wastewater: A mini review","authors":"Hasan M Agha,&nbsp;Ali H Jawad,&nbsp;Gianluca Viscusi,&nbsp;Hussein A Kazem","doi":"10.1002/jctb.70194","DOIUrl":"https://doi.org/10.1002/jctb.70194","url":null,"abstract":"<p>Synthetic dyes are among the most hazardous water pollutants because of their chemical structures, great stability, and resistance to natural degradation. Their common use in textile, paper, leather, and related industries contributes to profound ecological and human health risks, including toxicity, mutagenicity, and carcinogenicity. Conventional physicochemical and biological treatments often display limited efficiency against such recalcitrant pollutants, requiring the development of sustainable alternatives. This review critically examines the major classes of dyes, such as anthraquinone, cationic, nonionic, anionic, and azo, and evaluates current biological, chemical, physical, and hybrid treatment methods. Biological methods, including microbial and enzymatic degradation, are eco-friendly but restricted by dye selectivity and scalability. Chemical methods, such as advanced oxidation, Fenton reactions, and ozonation, achieve effective decolorization but remain cost intensive and generate secondary by-products that require further treatment. Physical strategies, particularly adsorption and membrane filtration, provide high removal efficiency but face challenges of regeneration, fouling, and sludge generation. Emerging hybrid systems that integrate material innovations with biological or chemical pathways demonstrate synergistic performance, offering enhanced dye removal with reduced environmental burden. The review emphasizes that no single method is universally effective; instead, future wastewater treatment requires integrated, low-cost, and eco-compatible technologies to ensure sustainable effluent management and protection of environmental and public health. © 2026 Society of Chemical Industry (SCI).</p>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"101 8","pages":"1461-1477"},"PeriodicalIF":2.7,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148386634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green treatment of concentrated sulfuric acid: mercury ion removal using adsorption with activated carbon","authors":"Eduard Lyudmilov Stefanov,&nbsp;Stela Ivanova Georgieva,&nbsp;Temenuzhka Hristova Radoykova","doi":"10.1002/jctb.70188","DOIUrl":"https://doi.org/10.1002/jctb.70188","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>The mercury (Hg) content in technical concentrated sulfuric acid must be strictly controlled for industrial applications, particularly in water treatment, fertilizer production, etc. This study explores the removal of Hg(II) ions directly from concentrated sulfuric acid (93–98%) using granular activated carbon. While adsorption processes are well established in aqueous systems, their application in concentrated sulfuric acid matrices has received limited attention due to the highly aggressive chemical media.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>Adsorption performance was evaluated through equilibrium studies, kinetic modeling and continuous-flow fixed-bed column experiments. Scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray fluorescence spectroscopy with energy-dispersive X-ray analysis confirmed the chemical stability of activated carbon under strongly acidic conditions and the adsorption of Hg(II) on the activated carbon. The Sips isotherm model provided the best description of equilibrium data (<i>R</i>² = 0.996), while Avrami kinetics adequately fitted the adsorption process (<i>R</i>² = 0.968).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>Fixed-bed column results further demonstrated practical feasibility, suggesting that the use of granular activated carbon offers a promising approach for Hg control in sulfuric acid production. © 2026 Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"101 8","pages":"1517-1530"},"PeriodicalIF":2.7,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148386914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using generalized quantitative structure–property relationship (QSPR) models to predict host cell protein retention in ion-exchange chromatography","authors":"Tim Neijenhuis,&nbsp;Olivier Le Bussy,&nbsp;Geoffroy Geldhof,&nbsp;Marieke E Klijn,&nbsp;Marcel Ottens","doi":"10.1002/jctb.70026","DOIUrl":"https://doi.org/10.1002/jctb.70026","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>Selecting an optimal chromatography resin during biopharmaceutical downstream process development is a great challenge. This is especially the case for recombinant subunit vaccines, where product properties vary greatly and recovery often involves cell lysis, which yields a complex mixture of different host cell materials. Host cell protein (HCP) impurities may remain similar for platform processes, but their critical impact on separation efficiency is relative to specific product properties. Therefore, every process needs to be designed per product. Prior knowledge on the elution behavior of HCPs would support the identification of critical compounds. However, determining chromatographic behavior of HCPs experimentally is a time-consuming approach.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>In this work, we leverage quantitative structure–property relationship (QSPR) models calibrated with retention data of 13 commercial proteins, collected at pH 7, 8, 9 and 10 to predict the anion-exchange retention of <i>Escherichia coli</i> HCPs. These models use features calculated from the molecular structure to describe protein behavior, like chromatographic retention. A multilinear regression model containing two features (isoelectric point and sum of negative surface electrostatics) was able to predict the retention times of 288 HCPs accurately (error ≤ 5%). Moreover, we identified the key attributes missing in the training dataset, which is important to increase model performance in the future.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>This work showcases how chromatographic data obtained using commercial proteins can be translated to a clarified <i>E. coli</i> lysate to accelerate chromatography resin selection for new products. © 2025 The Author(s). <i>Journal of Chemical Technology and Biotechnology</i> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"101 7","pages":"1420-1428"},"PeriodicalIF":2.4,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jctb.70026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148208870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing rapeseed protein purification: a continuous chromatographic approach for napin and cruciferin","authors":"Jonas Arnecke,&nbsp;Jessica Gillmann,&nbsp;Tim Börner,&nbsp;Mathias Hafner,&nbsp;Christian Frech","doi":"10.1002/jctb.70032","DOIUrl":"https://doi.org/10.1002/jctb.70032","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>Napin and cruciferin, the predominant storage proteins in <i>Brassica napus</i> (rapeseed), exhibit favorable techno-functional properties such as emulsification and gel formation, positioning them as promising candidates for the encapsulation and delivery of bioactive compounds. Their classification as GRAS (Generally Recognized As Safe), combined with their biodegradability and biocompatibility, highlights their potential as sustainable alternatives to synthetic delivery matrices. However, their broader application is hindered by the absence of scalable purification processes that concurrently ensure high purity and yield.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>This study presents the development of a fully continuous chromatographic platform for the simultaneous isolation of napin and cruciferin from crude rapeseed extracts. Napin was purified via cation-exchange chromatography, whereas cruciferin was isolated using anion exchange. Key process parameters were identified through systematic batch screening, with emphasis on the initial binding conditions to enable direct coupling of both chromatographic steps. The optimized protocol permitted efficient sequential protein capture with minimal buffer adjustment, leading to significantly reduced process time and resource consumption. The purified proteins exhibited high purity and are of suitable quality for future applications.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>The continuous purification process established in this study constitutes a significant step toward the scalable and sustainable processing of plant-derived proteins. By applying principles of process intensification, the method enhances overall operational efficiency and environmental compatibility. Importantly, the high-purity napin and cruciferin obtained through this platform provide a promising foundation for future development of bioactive compound encapsulation and delivery systems, supporting the broader integration of plant-based biopolymers in pharmaceutical and nutraceutical applications. © 2025 The Author(s). <i>Journal of Chemical Technology and Biotechnology</i> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"101 7","pages":"1370-1383"},"PeriodicalIF":2.4,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jctb.70032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148208935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pioneering the Next Generation of Bioseparations: selected insights from the Biopartitioning and Purification Conference 2024, Luxembourg","authors":"","doi":"10.1002/jctb.70201","DOIUrl":"https://doi.org/10.1002/jctb.70201","url":null,"abstract":"","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"101 7","pages":"1289-1290"},"PeriodicalIF":2.4,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148208963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixed-mode membrane chromatography: mechanistic-modeling-based optimization and scale-up of antibody charge variant separation using a novel membrane adsorber","authors":"Jan Hedrich,&nbsp;Rita Steigmiller,&nbsp;Viktor Simaev,&nbsp;Romas Skudas,&nbsp;Michael Schulte,&nbsp;Mathias Hafner,&nbsp;Christian Frech","doi":"10.1002/jctb.70043","DOIUrl":"https://doi.org/10.1002/jctb.70043","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; BACKGROUND&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Efficient manufacturing of complex biopharmaceuticals increasingly relies on innovative downstream processing strategies. Membrane chromatography offers a promising alternative to resin-based systems by enabling high-throughput separations at elevated flow rates. Typically used in flow-through mode, its industrial applications range from contaminant removal (DNA, host cell proteins) to purification of large biomolecules like viruses, virus-like particles and protein complexes. The Natrix® CH membrane, a novel mixed-mode membrane integrating ion-exchange and hydrophobic interaction functionalities, has shown potential in complex or elevated salt conditions (&lt;i&gt;ca&lt;/i&gt; 0.1 mol L&lt;sup&gt;−1&lt;/sup&gt;). This study evaluates its performance in bind-and-elute mode for separating monoclonal antibody charge variants and establishes a scalable mechanistic model.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; RESULTS&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Initial comparisons with a traditional ion-exchange membrane showed that the Natrix® CH had enhanced selectivity, as demonstrated by better separation of charge variants and an overall increase of retention up to one pH unit. Both might be attributed to its additional hydrophobic ligand. Linear gradient elution experiments enabled calibration of a stoichiometric displacement model under low-loading conditions. High-loading experiments up to 35 mg mL&lt;sub&gt;MV&lt;/sub&gt;&lt;sup&gt;−1&lt;/sup&gt; refined a steric mass action model using a reverse fitting procedure to capture the nonlinear range of the adsorption isotherm. These models were applied &lt;i&gt;in silico&lt;/i&gt; to design optimized separation conditions, including an antiparallel dual pH and salt gradient. This dual-gradient strategy achieved high-resolution variant separation and was validated experimentally. A scale-up from laboratory scale (1.06 mL) to a bench-scale (8.8 mL) membrane device (eightfold increase) demonstrated consistent elution behavior and separation performance, even at elevated flow rates (up to 10 membrane volumes per minute), with only minor reductions in dynamic binding capacity (e.g. DBC&lt;sub&gt;10%&lt;/sub&gt; decreased by 7%).&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; CONCLUSION&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Mechanistic &lt;i&gt;in silico&lt;/i&gt; modeling enabled accurate prediction, optimization and successful scale-up of charge variant separation on Natrix® CH membranes. The approach demonstrated good applicability and scalability within the calibrated range, advancing membrane chromatography as a high-throughput alternative for efficient protein purification. © 2025 The Author(s). &lt;i&gt;Journal of Chemical Technology and Biotechnology&lt;/i&gt; published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry (","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"101 7","pages":"1407-1419"},"PeriodicalIF":2.4,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jctb.70043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148208873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}